Improving the subcritical water extraction of flavonoids narirutin and hesperidin from Citrus unshiu peel by pulsed electric fields

Hui-Ju Kim,Mi-Ri Kwon,Hye-Ji Kang,Na-Yeon Kim,Hee-Jeong Hwang,Min-Jung Ko,Myong-Soo Chung 한국산업식품공학회 2017 학술대회 및 심포지엄 Vol.2017 No.11

Citrus fruit is important source of flavonoids, mainly flavanones which are narirutin and hesperidin. Those citrus flavonoids have been found to have health-related properties including antioxidant, anticancer, and anti-inflammatory. The main purpose of this study was to verify that the extraction of narirutin and hesperidin from Citrus peel can be more effective by combining pulsed electric field (PEF) pre-treatment and subcritical water extraction (SWE). Citrus unshiu peels were treated with PEF under conditions of electric field strength (3 kV/cm) and times (1 and 2 min). Subsequent SWE was conducted by using a Dionex Accelerated Solvent Extractor (ASE, Model 350) at extraction temperature 170°C for 10 min. The total flavonoids content was measured by using the aluminum chloride colorimetric method and the antioxidant capacity was analyzed by the Ferric reducing antioxidant power (FRAP) assay using spectrophotometer. The concentrations of narirutin and hesperidin were increased as PEF pre-treatment time increased. The highest concentrations of narirutin and hesperidin were 13.41 mg narirutin/g dry citrus peel and 141.16 mg hesperidin/g dry citrus peel at PEF pre-treatment condition of 3 kV/cm and 2 min. The total flavonoids contents of the extracts increased 105.2% and 123.1% for citrus peel PEF treated at 1 and 2 min, respectively. In addition, compared to the untreated sample, PEF pre-treatments of 1 and 2 min increased the antioxidant capacity of the extracts 109.2% and 160.8%, respectively. Therefore, the results demonstrate the potential of PEF pre-treatment to improve the SWE of flavonoids from citrus unshiu peel.

사회인구학적 변인에 따른 대학생들의 노인에 대한 태도와 노인부양의식에 관한 연구

고정자,이은민 동아대학교 생활과학연구소 2000 생활과학연구 논문집 Vol.8 No.-

The Purpose of this study is to offer a solution to support the aged by investigation Attitudes and Support consciousness toward the old according to socio-demographic variables of college students. First, how different are the attitudes toward old persons by socio-demographic variables of college students? Second. how different is the support consciousness for the aged by sucio-demographic variables of college students? Third, are there any relations between Support Consciousness and Attitudes to the old? For the subjects of this study 817 college students were selected among 5 Universities in Pusan. Questionnare was composed of socio-demographic variables, Attitudes toward old persons Scale and Support Consciousness for the aged Scale. The data were analyzed with the SPSS statistical pacakage using paired t-test, t-test, one way ANOVA, Scheffe's Multiple range test, Pearson correlation test. The major findings of this study can be summarized as follow: First, in general, college students tend to show positive attitudes toward old persons. They showed more positive attitudes on physical aspects, retirement and economical ablities and family relationship of old people than personality, adjustment and inter-relations of old people. The socio-demographic variables to have significant incluence on the total attitudes toward old people showed as religion, father's birth rank, the experience of cohibitation their grandparents, and home education. The socio-demographic variables to have significant influence on the physical aspects of old people showed as sex, religion, father's birth rank, the experience of living together with their grandparents, the living types of desiring with their parents after marrig and home education. The socio-demographic variables to have significant influence on the personality characteristics of old people showed as grade, religion, birth rank, the types of home education. The socio-demographic varlables t? have significant influence on the adjustment and inter-relations of old people showed as sex, grade, religion, father's birth rank, having two parents or not. The socio-demoraphic variables to have significant influence on the retirement and economical ablities of old people showed as sex, grade, religion, SES, birth rank, father's birth rank, the experience of living together with their grandparents, types of home education. The socio-demographic variables to have significant influence on the family relationships of old people showed as sex, grade, birth rank, father's birth rank, the living types of desiring with their parents after marrying. Second, in general, college students thens to show high level on support consiousness for the aged. They showed more high levels on emotional awareness than economical and physical-service awareness, The socio-demographic variables to have significant influence on the total support consciousness for the aged showed as grade, religion, father's birth rank, having two parents or not. The soclo-demoraphic variables to have significant influence on the economical support consciousness for the aged showed as grade, religion, SES, blrth rank, father's birth rank, the living types of desiring with their parents after marrying, the types of home education. The socio-demographic variables to have significant influence on the emotional support consciousness for the aged showed as having two parents or not, the types of home education. The socio-demograthic variables to have significant influence on the physical-service support Consciousness for the aged showed as religion, having two parents or not. Third, the mere they have positive Attitudes to the old, the higher Suppport consciouseness for the acted goes up.

뼈흡수유도호르몬이 ROS17/2.8세포로부터 Nitric Oxide 형성에 미치는 영향

고선일,김민성,한원정,김세원,김정근 대한구강악안면방사선학회 2005 Imaging Science in Dentistry Vol.35 No.3

Purpose : We performed the present study to investigate whether osteotropic hormomes play roles on the nitric oxide (NO) production in culture of ROS17/2.8 osteoblastic cells. Materials and Methods : The osteoblastic cell line ROS17/2.8 cells were cultured in F12 medium supplemented with 5% fetal bovine serum (FBS) at 37?C in a humidified atmosphere of 5% CO₂ in air. ROS17/2.8 cells were plated in 96-well plates at a density of 2-3×10³ cells/well and grown to confluence. Then the cells were pretreated with osteotropic hormones (parathyroid hormone (PTH) 20-500 ng/mL, 1,25 dihydroxycholecalciferol (1,25[OH]₂D₃) 1-100 nM; prostaglandin E₂ (PGE₂) 20-500 ng/mL) in the medium supplemented with 0.4% FBS for 72 hours and the cells were treated with cytokines (TNFα and IFNγ) in phenol red-free F12 medium for an additional 48 hours. NO synthesis was assessed by measuring the nitrite anion concentration, the reaction product of NO, in the cell culture medium using Griess reagent. Results : PTH and 1, 25[OH]₂D₃ pretreatment induced a significant increase in NO production in the presence of TNFα and IFNγ. PGE₂ slightly induced NO production compared to the control group. But, PGE2 pretreatment did not affect in NO production in the presence of TNFα and IFNγ. Conclusions : These results suggest that the actions of osteotropic hormones in bone metabolism may be partiallymediated by NO in the presence of cytokines.

김천대학 사회교육 활성화 방안에 관한 연구

고정환 ; 윤옥현 ; 박홍기 ; 전기환 ; 김상민 ; 김학민 ; 박희룡 김천대학교 1999 김천대학교 논문집 Vol.20 No.-

The social education that is provided for the people will be an important part of the national growth in the 21 century. This study was taken to present the desirable direction that Kimcheon College Continuing Education has to make progress as a central organization with perceiving the importance of continuing education for the Kimcheon citizen. To achieve the objective of the study, examined a sample of 592 citizen and students from Kimcheon City and Kimcheon College. The survey revealed the following results : 1. Offer the effective operation of education policies and curriculums. 2. Perform the role of foreign language education center. 3. Introduce the saving credit system. 4. Operate the special lectures for the students of the high school graduating class after the national scholastic achievement test for university and college entrance. 5. Suppert the special skills education for high school students after-school hours. 6. Administer the new employment program. These results showed the desirable direction that the Kimcheon College Continuing Education has to take some actions for the Kimcheon citizen.

사회인구학적 변인에 따른 대학생들의 노인에 대한 태도와 노인부양의식에 관한 연구

高貞子,李銀敏 東亞大學校 附設 生活科學硏究所 1998 생활과학연구 논문집 Vol.6 No.-

The Purpose of this study is to offer a solution to support the aged by investigating Attitudes and Supoort consciousness toward the old according to socio-demographic variables of college students. The data were analyzed with the SPSS statistical package using paired t-test, t-test, one way ANOVA, Scheffe's Multiple range test, Pearson Correlation test. The major findings of this study can be summarized as follow: First, in general, college students tend to show positive attitudes toward old persons. They showed more positive attitudes on physical aspects, retirement and economical ablities and family relationship of old people than personality, adjustment and inter-relations of old people. The socio-demographic variables to have significant influence on the total attitudes toward old people showed as religion, father's birth rank, the experience of cohibitation their grandparents and home education. The socio-demographic variables to have significant ingluence on the physical aspects of old people showed as sex, religion, father's birth rank, the experience of living together with their grandparents, the living types of desiring with their parents after marriage and home education. The socio-demographic variables to have significant influence on the personality characteristics of old people showed as grade, religion, birth rank, the types of home education. The socio-demographic variables to have significant influence on the adjustment and inter-relations of old people showed as sex, grade, religion, father's birth rank, having two parents or not. The socio-demographic variables to have significant influence on the retirement and economical ablities of old people showed as sex, grade, religion, SES, birth rank, father's birth rank, the experience of living together with their grandparents, the types of home education. The socio-demographic variables to thave significant influence on the family relationships of old people showed as sex, grade, birth rank, father's birth, the living types of desiring with their parents after marrying. Second, in general, college students tend to show high level on support consciousness for the aged. They showed more high levels on emotional awareness than economical and physical-service swareness. The socio-demographic variables to have significant influence on the total support consciousness for the aged showed as grade, religion, father's birth rank, haveing tow parents or not. The socio-demographic variables to have signigicant influence on the economical support consciousness for the aged showed as grade, religion, SES, birth rand, father's birth rank, the living types of desiring with their parents after marrying, the types of home education. The socio-demographic variables to have significant influence on the emotional suport consciousness for the aged showed as having two parents or not, the types of home education. The socio-demographic variables to have significant influence on the physical-service support Consciousness for the aged showed as religion, having two parents or not. Third, the more they have positive Attitudes to the old, the higher Support consciouseness for the aged goes up.

동종 조혈모세포이식 후 발생한 크립토콕쿠스 척추염 1예

고윤호,임동준,이성수,조유경,이동건,최정현,김유진,민창기,김동욱,박정미,김춘추,신완식 대한감염학회 2001 감염 Vol.33 No.4

Skeletal cryptococcosis is an uncommon infection. Cryptococcus is a common cause of meningitis and infects 7∼10% of patients with AIDS. As well as AIDS, the infection may be seen in association with leukemia, lymphoma, Hodgkin's disease, sarcoidosis, tuberculosis and diabetes, also in patients on steroid medication. But there is no case report of skeletal cryptococcosis following allogeneic hematopoietic stem cell transplantation. A 40-year-old woman was admitted to the hospital because of low back pain. She had chronic myelogenous leukemia for 2 years and underwent allogeneic hematopoietic stem cell transplantation 8 months ago. She have been treated with steroid and cyclosporine orally because of chronic graft versus host disease. On examination she was afebrile and had posterior lower lumbar tenderness. But, she had no reduced strength of low extremities. Open biopsy was underwent. Histology demonstrated budding, round-to-oval, refractile yeast-like organisms within debris. The results of a lumbar puncture were unremarkable and cerebrospinal fluid culture failed to grow bacteria and yeast. The patient was treated with amphotericin B (1 gram) and AmBisome□ (2.8 gram) over 6 weeks. Three months after cessation of therapy, the patient was doing well.(Korean J Infect Dis 33:298∼301, 2001)

정신분열증 환자와 우울증 환자의 MMPI-2 프로파일

고윤화,손용훈,윤정흠,오상우,이귀행,박민철 圓光大學校 醫科學硏究所 2008 圓光醫科學 Vol.23 No.2

Background: Minnesota multiphasic personality inventory-2(MMPI-2) is superior to its predecessor in differential diagnosis. this study was to investigate MMPI-2 profiles of the patients with schizophrenia and depression and to discriminate among two groups. Methods: The sample consisted of 35 patients with schizophrenia(male 24, female 11), 35 patients with depression(male 18, female 17) diagnosed by the diagnostic and statistical manual of mental disorders(DSM-IV) for schizophrenia & depression at department of neuropsychiatry in WonKwang University from January 2005 to December 2007. The data from the results were analyzed statistically by t-test and discriminant analysis. Results: The results were as follows. First, according to t-test results the comparison of schizophrenia patients and depression patients in MMPI-2 among two groups showed significant differences; the validity scales: F, F(P); The clinical scales: D, Pa; The restructured clinical scales: Rcd(dem), RC4, RC6, RC8; The content scales: ANX, DEP, BIZ; The supplementary scales: AGGR, PSYC, Mt, GF, O-H. Second, as a results discriminant analysis, BIZ, D were significant predictors in distinguishing patients with schizophrenia from patients with depression. Third, probability to be discriminated schizophrenia in schizophrenia was 91.4%, probability to be discriminated depression in depression was 85.7%. Conclusion: Finally, we remind readers that although the MMPI-2 may prove to be useful in the task of differential diagnosis, it should be used only in combination with other patient information. 배경: MMPI-2는 감별진단에서 기존의 MMPI 보다 더 우수하다. 본 연구는 정신분열증 환자와 우울증 환자의 MMPI-2 프로파일과 두 집단을 판별하는 연구를 하였다. 방법: 2005년 1월부터 2007년 12월까지 원광대학교 병원 신경정신과에서 정신분열증과 우울증으로 진단된 정신분열증 환자 35명(남성 24명, 여성 11명), 우울증 환자 35명(남성 18명, 여성 17명)이 본 연구에 참여하였다. 자료는 t-검증과 판별분석으로 분석하였다. 결과: 결과는 다음과 같다. 1. t-검증 결과, 두 집단간 유의미한 차이를 보인 척도들로는 타당도 척도에서 F, F(P) 척도, 임상척도에서 D, Pa, 척도, 재구성 임상척도에서 Rcd(dem), RC4, RC6, RC8척도 였다. 내용척도에서는 ANX, DEP, BIZ척도 였으며 보충척도에서는 AGGR, PSYC, MT, GF O-H척도 였다. 2. 판별 분석 결과로서, 정신분열증과 우울증을 구별하는데 가장 유의한 예언요소는 BIZ, D척도로 나타났다. 3. 판별함수를 통한 진단율은 정신분열증을 정신분열증으로 판별해주는 정확도는 91.4%였고, 우울증을 우울증으로 판별해주는 정확도는 85.7%였다. 결론: 본 연구 결과를 통해서 MMPI-2가 두 집단을 감별하는데 유용하다고 증명되었을지 모르지만 더욱 정확하게 감별하기 위해서 환자의 기타 정보를 사용해야 할 것이다.

천연한약재(목통, 삼릉, 치자) 추출물의 항산화효과 및 항균활성효과

김정균,강영미,엄광식,고영민,김태영 경상대학교 농업생명과학연구원 2003 농업생명과학연구 Vol.37 No.4

한약재로 많이 이용되고 있는 목통, 삼릉 및 치자의 항산화활성과 항균활성 정도를 검토하기 위해 한약재를 물, 70% methanol(MeOH) 및 100% MeOH로 추출한 후 각 추출물의 페놀화합물함량, 전자공여능, hydroxy radical 소거효과, reducing power 및 항균활성시험을 하였다. 그 결과 실험에 사용한 3가지 한약재 모두 항산화효과와 항균활성이 뛰어났으며, 특히 목통 70% MeOH 추출물이 그 효과가 가장 컸으며 다음이 100% MeOH, 물추출물 순이었다. Antioxidant activity and antimicrobial activity of medicinal plants extracts from 3 edible or medicinal plant were examined. The extracts from Akebia quinate Decaisne, Scirusfluviatilis A. Gray, Gardenia jasminoides for. grandiflora Makino showed comparatively strong antioxidative activity on test. Electron donatating abilities and hydroxy radical scavenging ablities of 70% methanol, 100% methanol extracts were higher than thoes of water extracts. Some extracts of medicinal plants showed antimicrobial effect, especially, the extracts showed antimicrobial effect in the 70% methanol higher than other extracts.

기분장애 환자의 스트레스와 정서반응 : 우울, 불안, 분노 반응

박민철,오상우,류미경,피상순,고정인 대한생물치료정신의학회 1999 생물치료정신의학 Vol.5 No.2

Objectives : This study was investigated to demonstrate the relationship between stress and emotional responses of depression, anxiety, and anger in patients with mood disorder.Methods : Fifty patients with major depressive disorder diagnosed by DSM-IV were compared to those with bipolar manic disorder. All subjects were evaluated for perceived stress, Beck depression inventory, dysfunctional attitude, state-trait anxiety, state-trait anger and anger expression. The data were analyzed by t-test, correlation, and multiple regression analysis.Results : The results were as follows.1. Depressive group had significantly higher score of perceived stressful life events, depression, state anxiety and trait anxiety than manic group.2. Perceived stress in depressive group was positively correlated with depression, anxiety, state and trait anger, anger expression and sex, however that in manic group was positively correlated with state anger and anger expression.3. In depressive group, trait anxiety, trait anger, age, and dysfunctional attitude predicted 70.6% of variance on depression, perceived stress predicted only 1.2%. Trait anxiety predicted 67.6% for state anxiety, and trait anger and trait anxiety predicted 41.5% for state anger, trait anger and sex predicted 34.0% for anger expression.4. In manic group, education and trait anger predicted 28.7% of variance of depression, and trait anger predicted 38.3% for state anxiety. Trait anger and education predicted 29.4% for state anger, and state anger and sex, state anxiety predicted 36.3% for anger expression.Conclusion : The results suggest that emotional responses to perceived stress in depressive group are related to depression, anxiety, and anger but those in manic group are related to anger.

Platelet-Derived Growth Factor가 백서두개관 세포군의 증식 및 교원합성에 미치는 영향

김기수,고성희,백정화,민병무,김관식,정동균 대한구강생물학회 1991 International Journal of Oral Biology Vol.15 No.2

To study the effect of platelet-derived growth factor(PDGF) on the replication and collagen synthesis of rat calvarial cells, five bone cell populations(I-V) were prepared from fetal rat calvaria by sequential enzyme digestion. After primary culture for 6-7 days, each bone cell population was collected and then population Ⅰ and Ⅱ, Ⅳ and Ⅴ were pooled together. And the cells were resuspended at 6-8×10^4 cells/㎝^2 and cultured for 2-3 days. The medium was changed to serum-free medium prior to addition of growth factor. The effect of PDGF on the cell proliferation was measured by the incorporation of [^3H]thymidine into DNA. Protein synthesis was determined by measurement of [^3H]proline incorporation into collagenase-digestible protein(CDP) and noncollagenous protein(NCP) according to the method of Peterkofsky and Die-gelmann(1971). The observed results were as follows. 1. PDGF at 10 ng/㎖ significantly increased the [^3H]thymidine incorporation into DNA in all bone cell populations. 2. PDGF at 30 ng/㎖ significantly increased the synthesis of NCP in population Ⅰ, Ⅱ and Ⅳ, Ⅴ. 3. PDGF had no effect on the synthesis of CDP but percent collagen synthesis was decreased significantly in population Ⅳ, Ⅴ. Taken together, the increase of protein synthesis by PDGF in rat calvarial cells was due to the incraese of NCP synthesis.