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Pilot-scale Subcritical Water Extraction of Decursin and Nodakenin from Angelica gigas Nakai
Mi-Ri Kwon,Hwa-Hyun Nam,Myong-Soo Chung 한국산업식품공학회 2017 학술대회 및 심포지엄 Vol.2017 No.04
Angelica gigas Nakai has been used for traditional Korean medicine which acts anti-inflammatories, protects the liver, and enhances the immune system. Subcritical water extraction (SWE) is an eco-friendly extraction method in which the pressure is applied between the boiling point and critical temperature of water that maintains the liquid state. The aim of study was to determine the optimal extraction conditions for subcritical water extraction of the decursin and nodakenin from Angelica gigas Nakai, comparing two scales, laboratory-scale (22 mL) and pilot-scale (8 L) devices to assess the feasibility of industrial applications. Extraction efficiencies were evaluated by measuring total polyphenol contents and DPPH radical scavenging activity with a spectrophotometer, extraction yields of decursin and nodakenin with a high-performance liquid chromatography. The subcritical water extraction conditions of pilot-scale were as follows: extraction temperature of 150, 170, 190 and 210°C; extraction times of 5, 10, 15, and 20 min. The highest yields of decursin and nodakenin were at 190°C/15 min (17.65 mg/g Angelica gigas) and 170°C/20 min (4.35 mg/g Angelica gigas), respectively. These were not significantly different from the maximum contents of decursin (13.49 mg/g Angelica gigas), and nodakenin (4.66 mg/g Angelica gigas) for lab-scale subcritical water extraction. The maximum values for total polyphenol content and DPPH scavenging activity of subcritical water extraction were 64.32 mg/g Angelica gigas and 74.4%, respectively. On the other hand, those of conventional extraction method using methanol (60°C/120 min) were obtained as 14.31 mg/g Angelica gigas and 41.9%. Therefore, the subcritical water extraction is a rapid and efficient method for extracting polyphenols and anti-oxidative compounds.
Kwon, Mi-Ri,Kim, Chan Kyo,Kim, Jae-Hun British Institute of Radiology 2017 The British journal of radiology Vol.90 No.1079
<P>Advance in knowledge: DWI using b = 1000 s mm(-2) instead of b = 1500 s mm(-2) reduces examination time or image distortion, with improved the signal-to-noise ratio.</P>
Kwon Mi-ri,Ko Eun Sook,Park Min Su,Jeong Woo Kyoung,Hwang Na Young,Kim Jae-Hun,Lee Jeong Eon,Kim Seok Won,Yu Jong Han,Han Boo-Kyung,Ko Eun Young,Choi Ji Soo,Park Ko Woon 대한영상의학회 2022 Korean Journal of Radiology Vol.23 No.2
Objective: This study aimed to investigate the impact of baseline values and temporal changes in body composition parameters, including skeletal muscle index (SMI) and visceral adipose tissue area (VAT), measured using serial computed tomography (CT) imaging on the prognosis of operable breast cancers in Asian patients. Materials and Methods: This study retrospectively included 627 Asian female (mean age ± standard deviation [SD], 53.6 ± 8.3 years) who underwent surgery for stage I–III breast cancer between January 2011 and September 2012. Body composition parameters, including SMI and VAT, were semi-automatically calculated on baseline abdominal CT at the time of diagnosis and follow-up CT for post-treatment surveillance. Serial changes in SMI and VAT were calculated as the delta values. Multivariable Cox regression analysis was used to evaluate the association of baseline and delta SMI and VAT values with disease-free survival. Results: Among 627 patients, 56 patients (9.2%) had breast cancer recurrence after a median of 40.5 months. The mean value ± SD of the baseline SMI and baseline VAT were 43.7 ± 5.8 cm2/m2 and 72.0 ± 46.0 cm2, respectively. The mean value of the delta SMI was -0.9 cm2/m2 and the delta VAT was 0.5 cm2. The baseline SMI and VAT were not significantly associated with disease-free survival (adjusted hazard ratio [HR], 0.983; 95% confidence interval [CI], 0.937–1.031; p = 0.475 and adjusted HR, 1.001; 95% CI, 0.995–1.006; p = 0.751, respectively). The delta SMI and VAT were also not significantly associated with disease-free survival (adjusted HR, 0.894; 95% CI, 0.766–1.043; p = 0.155 and adjusted HR, 1.001; 95% CI, 0.989–1.014; p = 0.848, respectively). Conclusion: Our study revealed that baseline and early temporal changes in SMI and VAT were not independent prognostic factors regarding disease-free survival in Asian patients undergoing surgery for breast cancer.
Kwon Jae-Woo,Kim Mi-Ae,Sim Da Woon,Lee Hwa Young,Rhee Chin Kook,Yang Min-Suk,심지수,김민혜,Kim So Ri,Park Chan Sun,Kim Byung-Keun,Kang Sung-Yoon,Choi Gil-Soon,Lee Hyun,Jang An-Soo,김상헌 대한천식알레르기학회 2022 Allergy, Asthma & Immunology Research Vol.14 No.3
Purpose: Oral corticosteroids (OCSs) are frequently prescribed for asthma management despite their adverse effects. An understanding of the pattern of OCS treatment is required to optimize asthma treatment and reduce OCS usage. This study evaluated the prescription patterns of OCSs in patients with asthma. Methods: This is a retrospective multicenter observational study. We enrolled adult (≥18 years) patients with asthma who had been followed up by asthma specialists in 13 university hospitals for ≥3 years. Lung function tests, the number of asthma exacerbations, and prescription data, including the days of supply and OCS dosage, were collected. The clinical characteristics of OCS-dependent and exacerbation-prone asthmatic patients were evaluated. Results: Of the 2,386 enrolled patients with asthma, 27.7% (n = 660) were OCS users (the median daily dose of OCS was 20 mg/day prednisolone equivalent to a median of 14 days/year). OCS users were more likely to be female, to be treated at higher asthma treatment steps, and to show poorer lung function and more frequent exacerbations in the previous year than non-OCS users. A total of 88.0% of OCS users were treated with OCS burst with a mean dose of 21.6 ± 10.2 mg per day prednisolone equivalent to 7.8 ± 3.2 days per event and 2.4 times per year. There were 2.1% (51/2,386) of patients with OCS-dependent asthma and 9.5% (227/2,386) with exacerbation-prone asthma. These asthma phenotypes were consistent over the 3 consecutive years in 47.1% of OCS-dependent asthmatic patients and 34.4% of exacerbation-prone asthmatic patients when assessed annually over the 3-year study period. Conclusions: We used real-world data from university hospitals in Korea to describe the OCS prescription patterns and relievers in asthma. Novel strategies are required to reduce the burden of OCS use in patients with asthma.
Kwon, Sun-Jung,Park, Mi-Ri,Kim, Ki-Woo,Plante, Carol A.,Hemenway, Cynthia L.,Kim, Kook-Hyung Elsevier 2005 Virology Vol.334 No.1
<P><B>Abstract</B></P><P>The 5′ region of <I>Potato virus X</I> (PVX) RNA containing an AC-rich single-stranded region and stem–loop 1 (SL1) has been shown to be important for PVX replication (Miller, E.D., Plante, C.A., Kim, K.-H., Brown, J.W., Hemenway, C., 1998. Stem–loop structure in the 5′ region of potato virus X genome required for plus-strand RNA accumulation. J. Mol. Biol. 284, 591–608.). Here, we describe the involvement of SL1 for binding to the PVX coat protein (CP) using an in vitro assembly system and various deletion mutants of the 5′ region of PVX RNA. Internal and 5′ terminal deletions of the 5′-nontranslated region of PVX RNA were assessed for their effects on formation of assembled virus-like particles (VLPs). Mutant RNAs that contain the top region of SL1 or sequences therein bound to CP to form VLPs. In contrast, transcripts of mutants that disrupt SL1 RNA structure were unable to form VLPs. SELEX was used to further confirm the specific RNA recognition of PVX CP using RNA transcripts containing randomized sequences of the upper portion of SL1. Wild-type (wt) sequences along with many other sequences that resemble SL1 structure were selected after fourth and fifth rounds of SELEX (27.0% and 44.4%, respectively). RNA transcripts from several SELEX winners that are predicted to form stable stem–loop structures very closely resembling wt PVX SL1 VLPs. RNA transcripts not predicted to form secondary structures similar to SL1 did not form VLPs in vitro. Taken together, our results suggest that RNA secondary structural elements within SL1 and/or sequences therein are crucial for formation of VLPs and are required for the specific recognition by the CP subunit.</P>
Kwon Hye Ri,Kyu Sik Yoon,Min Ah Kang,Min Woo Park,Shin Hyuk Jo,Shin Hyo Seob,Sae Hee Kim,Eun Jin Kang,Mi Ja Seo,Yong Man Yu,Young Nam Youn 한국응용곤충학회 2010 한국응용곤충학회 학술대회논문집 Vol.2010 No.10
Several species of the genus Aphidius are used in biological control programs against aphid pests throughout the world and their behavior and physiology are well studied. But despite knowing the importance of sensory organs in their behavior, their antennal structure is largely unknown. In this study, the external morphology and distribution of the antennal sensilla on the antennal of both female and male adults of A. colemani were described using scanning electron microscopy (SEM). Generally, the filaform antennae of males (1,515.20±116.48 ㎛) are longer than females (1,275.06±116.42㎛). Antennae of this species is made up of scape, pedicel and flagellomeres. Male and female antennae differed in the total number of flagellomeres as 15 in males and 13 in females. Female and male antennae of A. colemani has samely seven types of sensilla. We classified sensilla placodea, Bohm bristles, 2 types of sensilla coeloconica, , 2 types of sensilla basiconica as with a tip pore and with wall pores, sensilla trichodea. In addition, the possible functions of the above sensilla types are discussed in light of previously published literature; mechanoreception(Bohm bristles, sensilla coeloconicaⅡ and sensilla trichodea) and chemoreception(sensilla coeloconicaⅠ, sensilla basiconicaⅠ,Ⅱ and sensilla placodea). Future studies on the functional morphology of the antennal sensilla of A. colemani using transmission electron microscopy (TEM) coupled with electrophysiological recordings will likely confirm the functions of the different sensilla identified in this study.
Kwon Yong Shik,Park Sun Hyo,Kim Hyun Jung,Lee Ji Yeon,Hyun Mi-ri,Kim Hyun ah,박재석 대한의학회 2020 Journal of Korean medical science Vol.35 No.26
There is still a paucity of studies on real-world outcome of screening clinic for hospital protection from coronavirus disease 2019 (COVID-19). As the number of COVID-19 cases was growing rapidly in Daegu, Korea, we started operating an active screening clinic outside the hospital premises. Over two weeks, 2,087 patients were screened using real- time reverse transcriptase polymerase chain reaction testing for severe acute respiratory syndrome coronavirus 2, with 42 confirmed cases. Before the screening clinic period, an average of 36 beds (maximum 67 beds) per day were closed due to unrecognized COVID-19 patients entering the hospital. In contrast, after the screening clinic operated well, only one event of closing emergency room (25 beds) occurred due to a confirmed COVID-19 case of asymptomatic patient. We report the operational process of screening clinic for COVID-19 and its effectiveness in maintaining the function of tertiary hospitals.
Anti-inflammatory and antioxidant effects of umbelliferone in chronic alcohol-fed rats
Mi-Ok Sim,Hae-In Lee,Ju Ri Ham,Kwon-Il Seo,Myung-Joo Kim,Mi-Kyung Lee 한국영양학회 2015 Nutrition Research and Practice Vol.9 No.4
BACKGROUND/OBJECTIVES: Inflammation is associated with various types of acute and chronic alcohol liver diseases. In this study, we examined whether umbelliferone (7-hydroxycoumarin, UF) ameliorates chronic alcohol-induced liver damage by modulating inflammatory response and the antioxidant system. METHODS: Rats were fed a Liber-Decarli liquid diet containing 5% alcohol with or without UF (0.05 g/L) for 8 weeks, while normal rats received an isocaloric carbohydrate liquid diet. RESULTS: Chronic alcohol intake significantly increased serum tumor necrosis factor-α (TNF-α) and interleukin 6 levels and decreased interleukin 10 level; however, UF supplementation reversed the cytokines related to liver damage. UF significantly suppressed hepatic lipopolysaccharide binding protein, toll-like receptor 4 (TLR4), nuclear factor kappa B, and TNF-α gene expression increases in response to chronic alcohol intake. Masson"s trichrome staining revealed that UF improved mild hepatic fibrosis caused by alcohol, and UF also significantly increased the mRNA expressions and activities of superoxide dismutase and catalase in liver, and thus, decreased lipid peroxide and mitochondrial hydrogen peroxide levels. CONCLUSIONS: The findings of this study indicate that UF protects against alcohol-induced liver damage by inhibiting the TLR4 signaling pathway and activating the antioxidant system.