Phytoecdysteroid가 조골세포와 파골세포의 성장과 활성에 미치는 영향

고선일,Ko, Seon-Yle 대한안면통증구강내과학회 2007 Journal of Oral Medicine and Pain Vol.32 No.2

Ecdysteroids are known as insect molting hormone. At the same time, ecdysteroids and plant ecdysteroids (phytoecdysteorids) reveal beneficial effects on mammal. The present study was undertaken to determine the possible cellular mechanism of action of phytoecdysteroids in bone metabolism. The effects on the osteoblasts were determined by measuring cell proliferation, alkaline phosphatase (ALP) activity, and gelatinase activity. The effects on the osteoclasts were investigated by measuring tartrate-resistant acid phosphatase (TRAP)(+) multinucleated cells (MNCs) formation after culturing osteoclast precursors. Phytoecdysteroid treatment showed a increase in ALP activity of osteoblasts. Phytoecdysteroid increased the activity of gelatinase. In addition, phytoecdysteroid decreased the osteoclast generation induced by macrophage-colony stimulating factor (M-CSF) and receptor activator of NF-kB ligand (RANKL) in (M-CSF)-dependent bone marrow macrophage (MDBM) cell cultures. Taken these results, phytoecdysteroid may be a regulatory protein within the bone marrow microenvironment.

뼈흡수유도호르몬이 ROS17/2.8세포로부터 Nitric Oxide 형성에 미치는 영향

고선일,김민성,한원정,김세원,김정근 대한구강악안면방사선학회 2005 Imaging Science in Dentistry Vol.35 No.3

Purpose : We performed the present study to investigate whether osteotropic hormomes play roles on the nitric oxide (NO) production in culture of ROS17/2.8 osteoblastic cells. Materials and Methods : The osteoblastic cell line ROS17/2.8 cells were cultured in F12 medium supplemented with 5% fetal bovine serum (FBS) at 37?C in a humidified atmosphere of 5% CO₂ in air. ROS17/2.8 cells were plated in 96-well plates at a density of 2-3×10³ cells/well and grown to confluence. Then the cells were pretreated with osteotropic hormones (parathyroid hormone (PTH) 20-500 ng/mL, 1,25 dihydroxycholecalciferol (1,25[OH]₂D₃) 1-100 nM; prostaglandin E₂ (PGE₂) 20-500 ng/mL) in the medium supplemented with 0.4% FBS for 72 hours and the cells were treated with cytokines (TNFα and IFNγ) in phenol red-free F12 medium for an additional 48 hours. NO synthesis was assessed by measuring the nitrite anion concentration, the reaction product of NO, in the cell culture medium using Griess reagent. Results : PTH and 1, 25[OH]₂D₃ pretreatment induced a significant increase in NO production in the presence of TNFα and IFNγ. PGE₂ slightly induced NO production compared to the control group. But, PGE2 pretreatment did not affect in NO production in the presence of TNFα and IFNγ. Conclusions : These results suggest that the actions of osteotropic hormones in bone metabolism may be partiallymediated by NO in the presence of cytokines.

Effect of Pyrroloquinoline Quinone on Osteoclast Generation and Activity

고선일,한동호,김정근,Ko, Seon-Yle,Han, Dong-Ho,Kim, Jung-Keun Korean Academy of Orofacial Pain and Oral Medicine 2005 Journal of Oral Medicine and Pain Vol.30 No.3

We examined the effect of PQQ, as a scavenger of superoxide, on osteoclast-like cell formation and on mature osteoclast function. To determine whether PQQ scavenges the superoxide, nitroblue tetrazolium (NBT) staining, which is a method to detect superoxide, was performed on HD-11 cells which are a chick myelomonocytic cell line having tartrate-resistant acid phosphatase (TRAP) activity in response to 1,25-dihydroxyvitamin $D_3\;[1,25(OH)_2D_3]$. Histochemical study of TRAP was also performed on HD-11 cells. PQQ inhibited the TRAP-positive multinucleated cell formation of chicken bone marrow cells was also examined. The addition of 20 ${\mu}M$ PQQ inhibited the formation of TRAP-positive multinucleated cell. When chicken osteoclasts were cultured on dentin slices, treatment of 20 ${\mu}M$ PQQ resulted in a significant decrease in dentin resorption by osteoclasts in terms of total resorption area and number of resorption pits. The present data suggest that PQQ, possibly as a scavenger of superoxide ion, inhibits the osteoclastic differentiation and bone resorption.

Angiopoietin-2가 조골세포와 파골세포의 성장과 활성에 미치는 영향

고선일,Ko, Seon-Yle 대한안면통증구강내과학회 2006 Journal of Oral Medicine and Pain Vol.31 No.1

The present study was undertaken to determine the possible cellular mechanism of action of angiopoietin-2 in bone metabolism. The effects on the osteoblasts were determined by measuring 1) cell viability, 2) alkaline phosphatase (ALP) activity, 3) gelatinase activity, and 4) nitric oxide production. The effects on the osteoclasts were investigated by measuring 1) tartrate-resistant acid phosphatase (TRAP)(+) multinucleated cells (MNCs) formation, and 2) resorption areas after culturing osteoclast precursors. Angiopoietin-2 treatment showed a significant increase in both the viability and ALP activity of osteoblasts. Angiopoietin-2 increased the activity of gelatinase and nitric oxide production. In addition, angiopoietin-2 decreased the osteoclast generation induced by macrophage-colony stimulating factor (M-CSF) and receptor activator of NF-kB ligand (RANKL), and inhibited osteoclastic activity in (M-CSF)-dependent bone marrow macrophage (MDBM) cell cultures. Taken these results, angiopoietin-2 may be a regulatory protein within the bone marrow microenvironment.

Baicalin이 조골세포의 생성 및 활성에 미치는 영향

고선일,Ko, Seon-Yle 대한안면통증구강내과학회 2008 Journal of Oral Medicine and Pain Vol.33 No.2

Baicalin is a flavonoid compound isolated from the medicinal plant Scutellaria baicalensis. It is known to affect multiple biological functions, including of antibacterial, anti-viral, anti-inflammatory and analgesic effects. Baicalin can inhibit nuclear factor-kappaB activation. It has been reported that some flavonoids possess the effects of bone metabolism. The present study was undertaken to determine the possible cellular mechanism of action of baicalin in osteoblasts. The effects on the osteoblast were determined by measuring cell proliferation, cell viability, alkaline phosphatase activity, and osteoprotegerin secretion. Baicalin has no effect on the osteoblastic cell proliferation and cell viability. Baicalin treatment showed increase in alkaline phosphatase activity and osteoprotegerin secretion of osteoblasts. Thus, baicalin may be a regulatory protein within the bone.

하지불안증후군을 경험하는 혈액투석 환자를 위한 하지복합운동 프로그램 적용 효과

고선일 ( Ko Seon Il ),강경자 ( Kang Kyung Ja ) 병원간호사회 2020 임상간호연구 Vol.26 No.3

Purpose: This experimental study with nonequivalent control group and pre/post-tests aims to investigate the effects of a complex leg exercise program on severity of restless legs syndrome, sleep quality, depression, and physical performance in patients with maintenance hemodialysis. Methods: Patients in the experimental group were provided with the leg exercise program three times a week for eight weeks, a total of 24times, through watching videos during hemodialysis from August 1, 2017 to October 25. The experimental group and control group consisted of 22 people each. Data were analyzed using descriptive statistics, x² test, Fisher's exact test, and independent t-test utilizing SPSS/WIN 22.0. Results: After providing with the leg exercise program, these were level of restless legs syndrome and depression were significantly decreased in the experimental group (t=2.79, p=.032; t=0.53, p=.036, respectively). However, sleep quality and physical performance did not have significant difference between the two groups (t=0.02, p=.947; t=1.74, p=.957, respectively). Conclusion: A complex leg exercise program appears to be beneficial in improving restless legs syndrome and depression in maintenance hemodialysis patients with restless legs syndrome.

골조직 세포에서 Estrogen의 효과에 대한 Nitric Oxided의 역할

고선일(Seon-Yle Ko) 대한치과의사협회 2001 대한치과의사협회지 Vol.39 No.11

Interleukin-1β와 tumor necrosis factor-α가 마우스 조골세포의 nitric oxide 생성에 미치는 영향

채종성,고선일,김정근 단국대학교 치의학연구소 1996 논문집 Vol.8 No.1

Bone remodeling is characterized by the coupling of osteoclast-mediated bone resorption and osteoblast-mediated bone formation. The process is tightly regulated at the local level by an incompletely known complex network of humoral factors produced in the bone microenvironment, including peptide as well as non-peptide molecules. Nitric oxide(NO) is recently identified messenger molecule regulating a wide range of functions throughout the body but little is known about its possible role in skeletal metabolism. Many other cells, including macrophages, hepatocytes, chondrocytes and bone marrow cells, also produce NO. Recently, NO was reported to inhibit osteoclastic bone resorption in vitro, and there are indications of an arginine-dependent NO pathway in osteoblasts and osteoclasts. In this study we present evidence that mouse osteoblasts can be induced to produce NO by cytokines (interleukin-1β (IL-1β), tumor necrosis factor-α (TNT-α) known to modulate bone cell activity. The observed results were as follows. 1. In case of 24 hr incubation with IL-1β, NO production of MC3T3/E1 cells was not stimulated. But significant amounts of NO were measured after 48 hr incubation. In case of TNT-α, significant amounts of NO were measured after 24 hr, and increased further during the next 48 hr followed by a much slower increase up to 96 hrs. 2. IL-1β stimulated NO production into cultured medium in cultures of MC3T3/E1 cells dose dependently (0.4, 0.2, 1 ng/ml). 3. TNT-α stimulated NO production into cultured medium in cultures of MC3T3/E1 cells dose dependently (0.2, 1, 5 ng/ml). 4. Cytokine combination increased NO production dose dependently. And synergistic effects of the two cytokines were observed at all dose combinations.

Endothelin-1이 HOS 세포의 증식과 활성에 미치는 영향

배문서,고선일,김정근,김세원,Bae, Moon-Seo,Ko, Seon-Yle,Kim, Jung-Keun,Kim, Se-Won 대한안면통증구강내과학회 2001 Journal of Oral Medicine and Pain Vol.26 No.4

Endothelin-1 (ET-1) is a recently discovered potent vasoconstrictive peptide. It was first identified in vascular endothelial cells. ET-1 is a 21-amino acid peptide and elicits systemic effects such as stimulation of the production of atrial natriuretic peptide and release of aldosterone and corticosterone. In this study, to examine the role of ET-1 in the bone metabolism, effect of ET-1 on the proliferation and activity of osteoblastic cells was studied using HOS cells as osteoblast model. ET-1 dose-dependently increased the cell proliferation as determined by cell counting and MTT reduction assay after 48hr treatment. Alkaline phosphatase activity was inhibited by ET-1 and showed significant inhibition by 50 and 100 nM ET-1. ET-1 increased NBT reduction by HOS cells dose-dependently showing that ET-1 may increase the superoxide production by osteoblasts. Nitrite concentration in the media of HOS cell culture without cytokine stimulation was negligible and unaffected by ET-1 after 48hr treatment. Finally, after collection and concentration of conditioned media, gelatinase activity produced by HOS cells was determined by zymography. HOS cells can produce and secrete the gelatinase (gelatinase A type as determined by molecular weight of about 65,000) into culture media, however, ET-1 had no effect on the gelatinase activity. These findings suggest that ET-1 may have diverse effects on the proliferation and differentiation of osteoblasts, therefore, it may play an important role in bone metabolism.

Effects of Cytokines on the Osteoclast Formation from Mouse Bone Marrow Cells in Culture

Woo, Myung-Soo,Ko, Seon-Yle,Kim, Jung-Keun 단국대학교 치의학연구소 1996 논문집 Vol.8 No.1

최근 cytokine의 생물학적 활성이 골대사와 중요한 관련이 있다는 연구가 이루어지고 있으며, interleukin-1β (IL - 1β)와 tumor necrosis factor α (TNFα)는 골흡수를 촉진시키며 골형성을 억제하는 물질로 알려져 있다. 또한 최근 TNFα에 의해 유도된 골흡수가 interferon γ(INFγ)와 dexamethasone에 의해 억제되며, 부갑상선호르몬과 transforming growth factor β에 의해 증가된다는 보고가 있다. 따라서 본 연구에서는 IL-1β, TNFα와 INFγ등 수종의 cytokine이 마우스 골수세포로부터 파골세포의 형성에 미치는 영향을 관찰하고자 하였다. Cytokine이 파골세포 형성에 관여하는지 알아보기 위하여 마우스 골수세포를 분리하여 10 nM 1,25-dihydroxyvitamin D_3 존재하에 IL_1β, TNFα와 IFNγ를 처치하여 배양하였다. 파골세포의 표지효소인 tartrate resistant acid phosphatase (TRAP) 조직 화학 검사를 시행하여 TRAP 양성 다핵세포를 파골세포로 판정하였다. 1 ng/ml의 IL-1β와 20 ng/ml의 TNFα를 처치한 경우 TRAP 양성 다핵세포의 수가 증가된 반면 500 U/ml의 IFNγ를 처치한 경우 억제되었다. 그러나 골수세포 배양시 TNFα와 IFNγ를 복합 처치한 경우 파골세포의 형성이 상승적인 증가를 나타내었다. 위의 결과 cytokine의 단독 처치는 IL-1β와 TNFα에 의해 파골세포 형성을 촉진시키거나 IFNγ에 의해 억제되는 반면, TNFα에 의한 파골세포 유사세포의 형성은 IFNγ에 의해 상승적으로 증가되었다.