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( Jing Wang ),( Wei Chen ),( Fang Wang ),( Dong Wu ),( Jia Ming Qian ),( Jun Ren Kang ),( Hai Long Li ),( En Ling Ma ) 한국임상영양학회 2015 Clinical Nutrition Research Vol.4 No.2
Mitochondrial neurogastrointestinal encephalopathy (MNGIE) is characterized by significant gastrointestinal dysmotility. Early and long-term nutritional therapy is highly recommended. We report a case of MNGIE in a patient who was undergoing longterm nutrition therapy. The patient was diagnosed with a serious symptom of fatty liver and hyperlipidemia complications, along with homozygous mutation of the thymidine phosphorylase (TYMP) gene (c.217G > A). To our knowledge, this is the first report of such a case. Herein, we describe preventive measures for the aforementioned complications and mitochondrial disease-specific nutritional therapy.
Lingli Long,Jingnan Wang,Ningning Chen,Shuhui Zheng,Lanying Shi,Yuxia Xu,Canqiao Luo,Yubin Deng 한국식품영양과학회 2016 Journal of medicinal food Vol.19 No.6
The objective of our study was to investigate whether curcumin protects against reserpine-induced gastrointestinal mucosal lesions (GMLs) in rats and to explore the mechanism of curcumin’s action. Sprague-Dawley rats were randomly divided into four groups: control group, reserpine-treated group, reserpine treatment group with curcumin at high dose (200 mg/kg), and reserpine treatment group with curcumin at low dose (100 mg/kg). Rats in reserpine-treated group were induced by intraperitoneally administered reserpine (0.5 mg/kg) for 28 days. TUNEL staining and hematoxylin and eosin staining were used to evaluate the apoptotic cells and morphologic changes. In addition, to explore the mechanism of curcumin in protecting GMLs, we used serum of experimental rats to assess the level of vasoactive intestinal peptide (VIP), gastrin, interleukin-6, interleukin-10, tumor necrosis factor-α and interferon-γ by ELISA and radioimmunoassay. The protein levels of NF-κB, p-IκB-α, IκB-α, Bcl-2, Bax, and cleaved-caspase-3 were examined by western blot analysis. Data were analyzed with SPSS 19.0 software package. Curcumin treatment prevented tissue damage and cell death in the reserpinetreated rats and effectively decreased inflammatory response and balanced the expression of VIP and gastrin in the reserpinetreated rats. NF-κB, p-IκB-α, Bax, and cleaved-caspase-3 were increased in the reserpine group, but the curcumin high-dose group inhibited them. Curcumin can target the IκB-α/NF-κB pathway to inhibit inflammatory response and regulate the level of VIP and gastrin in reserpine-induced GML rats.
Lingli Long,Zhang Sheng,Junquan Zhu 한국통합생물학회 2015 Animal cells and systems Vol.19 No.3
Ultrastructural characteristics of spermiogenesis in the peanut worm, Phascolosoma esculenta (Phascolosomatidea), were observed by transmission electron microscopy (TEM). The spermiogenesis principally occurs in the coelom and can be divided into three stages (the early, middle and late stages) based on the chromatin morphology, acrosome development, spermatozoon midpiece and flagellum formation. Spermatids within a given spermatid mass develop synchronously. With the spermiogenesis proceeding, the spermatid masses become loosely structured, and later, adjacent spermatids are interconnected at one extremity of the cells. Gradually, condensation of the chromatin accelerates and is almost completed in late spermiogenesis, leaving the late spermatids with highly condensed homogeneous chromatin. In the spermatid head, the conical acrosome is generally composed of an acrosomal vesicle which is formed by the coalescence of small proacrosomal vesicles within the cytoplasm, a subacrosomal space that situates between the acrosomal vesicle and nucleus, and an acrosomal rod which develops from a bunch of filamentous material within the subacrosomal space. Certain mitochondria move posteriorly towards the nucleus, thus constituting the spermatozoon midpiece. The flagellum, originated from the distal centriole, appears in the early spermiogenesis. Ultimately, mature spermatidium dissociates into numerous spermatozoa, which are subsequently released as a single cell from the coelom into the nephridia. The spermatozoon has a prominent head, containing an acrosome and nucleus, a short midpiece and a slender tail. When compared with other sipunculans or invertebrates with external fertilization, the spermiogenesis of P. esculenta, presumably, is closely associated with its biological adaptations for the reproductive strategy.
Mao-Long Chen,Tian-Hui Lu,Ling-Li Long,Zhou Xu,Li Ding,Yun-Hui Cheng 대한환경공학회 2022 Environmental Engineering Research Vol.27 No.2
This investigation enables amino-functionalized metal–organic frameworks (MOFs) materials for the removal of imidacloprid (IMC). Two Fe-based MOF materials of NH₂-MIL-88B(Fe) and NH₂-MIL-101(Fe) both exhibited high adsorption capacity and Fenton-like degradation ability for IMC which were utilized to remove IMC from aqueous solution. Although the adsorption capacity of NH₂-MIL-101(Fe) was higher than that of NH₂-MIL-88(Fe), the degradation abilities of both MOF materials were similar. The removal efficiencies were evaluated through several basic studies, including concentrations of catalyst (0.12-0.3 g/L) and IMC (20-100 mg/L), pH of solution (3-11), and amounts of 30% H₂O₂ (0-2.0 μL/mL). By optimizing the above factors, the total removal ratio of IMC by NH₂-MIL-88B(Fe) was as high as 93%, whereas the removal ratio of NH₂-MIL-101(Fe) was 97%. Moreover, these MOF materials were proven to be stable and recyclable. The free radical quenching experiment and density functional theory calculation were applied to research the removal mechanism, and the hydroxyl radicals (·OH) was found to be the key active intermediate. The high catalytic efficiency can be attributed to the synergy of the Fe<SUP>3+</SUP>/Fe<SUP>2+</SUP> redox cycle.
Pituitary P62 deficiency leads to female infertility by impairing luteinizing hormone production
Li Xing,Zhou Ling,Peng Guiliang,Liao Mingyu,Zhang Linlin,Hu Hua,Long Ling,Tang Xuefeng,Qu Hua,Shao Jiaqing,Zheng Hongting,Long Min 생화학분자생물학회 2021 Experimental and molecular medicine Vol.53 No.-
P62 is a protein adaptor for various metabolic processes. Mice that lack p62 develop adult-onset obesity. However, investigations on p62 in reproductive dysfunction are rare. In the present study, we explored the effect of p62 on the reproductive system. P62 deficiency-induced reproductive dysfunction occurred at a young age (8 week old). Young systemic p62 knockout (p62 -/- ) and pituitary-specific p62 knockout (p62 flox/flox αGSU cre ) mice both presented a normal metabolic state, whereas they displayed infertility phenotypes (attenuated breeding success rates, impaired folliculogenesis and ovulation, etc.) with decreased luteinizing hormone (LH) expression and production. Consistently, in an infertility model of polycystic ovary syndrome (PCOS), pituitary p62 mRNA was positively correlated with LH levels. Mechanistically, p62 -/- pituitary RNA sequencing showed a significant downregulation of the mitochondrial oxidative phosphorylation (OXPHOS) pathway. In vitro experiments using the pituitary gonadotroph cell line LβT2 and siRNA/shRNA/plasmid confirmed that p62 modulated LH synthesis and secretion via mitochondrial OXPHOS function, especially Ndufa2, a component molecule of mitochondrial complex I, as verified by Seahorse and rescue tests. After screening OXPHOS markers, Ndufa2 was found to positively regulate LH production in LβT2 cells. Furthermore, the gonadotropin-releasing hormone (GnRH)-stimulating test in p62 flox/flox αGSU cre mice and LβT2 cells illustrated that p62 is a modulator of the GnRH-LH axis, which is dependent on intracellular calcium and ATP. These findings demonstrated that p62 deficiency in the pituitary impaired LH production via mitochondrial OXPHOS signaling and led to female infertility, thus providing the GnRH-p62-OXPHOS(Ndufa2)-Ca 2+ /ATP-LH pathway in gonadotropic cells as a new theoretical basis for investigating female reproductive dysfunction.
Ling, Jin,Yu, Yingjia,Long, Jiakun,Li, Yan,Jiang, Jiebing,Wang, Liping,Xu, Changjiang,Duan, Gengli The Korean Society of Ginseng 2019 Journal of Ginseng Research Vol.43 No.4
Background: 20(S)-Protopanaxadiol (PPD), the aglycone part of 20(S)-protopanaxadiol ginsenosides, possesses antidepressant activity among many other pharmacological activities. It is currently undergoing clinical trial in China as an antidepressant. Methods: In this study, an ultra-performance liquid chromatography coupled with triple quadrupole time-of-flight mass tandem mass spectrometry method was established to identify the metabolites of PPD in human plasma and urine following oral administration in phase IIa clinical trial. Results: A total of 40 metabolites in human plasma and urine were identified using this method. Four metabolites identified were isolated from rat feces, and two of them were analyzed by NMR to elucidate the exact structures. The structures of isolated compounds were confirmed as (20S,24S)-epoxydammarane-12,23,25-triol-3-one and (20S,24S)-epoxydammarane-3,12,23,25-tetrol. Both compounds were found as metabolites in human for the first time. Upon comparing our findings with the findings of the in vitro study of PPD metabolism in human liver microsomes and human hepatocytes, metabolites with m/z 475.3783 and phase II metabolites were not found in our study whereas metabolites with m/z 505.3530, 523.3641, and 525.3788 were exclusively detected in our experiments. Conclusion: The metabolites identified using ultra-performance liquid chromatography coupled with triple quadrupole time-of-flight mass spectrometry in our study were mostly hydroxylated metabolites. This indicated that PPD was metabolized in human body mainly through phase I hepatic metabolism. The main metabolites are in 20,24-oxide form with multiple hydroxylation sites. Finally, the metabolic pathways of PPD in vivo (human) were proposed based on structural analysis.
Laser Joining of Ti3Al-Based Alloy to Ni-Based Superalloy using a Titanium Interlayer
Xiao-Long Cai,Da-Qian Sun,Hong-Mei Li,Hong-Ling Guo,Yan Zhang,Ying-ying Che 한국정밀공학회 2018 International Journal of Precision Engineering and Vol.19 No.8
Joining Ti3Al-based alloy to Ni-based superalloy is of great interest for applications in the aerospace fields. Direct welding of these two materials was very difficult and the joint usually fractured during laser welding which cannot form an effective welding joint. In this work, a pure titanium interlayer with a thickness of 0.4 mm was used between the base metals. The results indicated that the addition of titanium interlayer has a great effect on the joint performance. No macrocracks were found through the whole joint. The average room-temperature tensile strength of the joint with titanium was 177 MPa which still much lower than the two base metals. The weak link of the dissimilar joint was the Ti3Al/weld interface. The presence of Ti2Ni, α2-Ti3Al and AlNi2Ti brittle intermetallic compounds in the Ti3Al/weld interface deteriorated the joint properties.