Case Report : Nutrition Therapy for Mitochondrial Neurogastrointestinal Encephalopathy with Homozygous Mutation of the TYMP Gene

( Jing Wang ),( Wei Chen ),( Fang Wang ),( Dong Wu ),( Jia Ming Qian ),( Jun Ren Kang ),( Hai Long Li ),( En Ling Ma ) 한국임상영양학회 2015 Clinical Nutrition Research Vol.4 No.2

Mitochondrial neurogastrointestinal encephalopathy (MNGIE) is characterized by significant gastrointestinal dysmotility. Early and long-term nutritional therapy is highly recommended. We report a case of MNGIE in a patient who was undergoing longterm nutrition therapy. The patient was diagnosed with a serious symptom of fatty liver and hyperlipidemia complications, along with homozygous mutation of the thymidine phosphorylase (TYMP) gene (c.217G > A). To our knowledge, this is the first report of such a case. Herein, we describe preventive measures for the aforementioned complications and mitochondrial disease-specific nutritional therapy.

Robust Control Guaranteeing Uniform Ultimate Boundedness for a Class of Mismatched Uncertain Parabolic Distributed Parameter Systems

Hai-Long Xing,Dong-Hai Li,Wen-Shan Cui 제어·로봇·시스템학회 2013 International Journal of Control, Automation, and Vol.11 No.4

The robust control problem for a class of mismatched uncertain parabolic distributed parameter systems is studied under conditions of the uncertain terms being norm-bounded. The proposed robust controller can guarantee the system states to be the uniform ultimate boundedness, and their bound can be arbitrarily small. And, the proposed control method is successfully applied to a typical diffusion-reaction process with uncertain terms.

The Effect of Amino Density on the Attachment, Migration, and Differentiation of Rat Neural Stem Cells In Vitro

Hai-Long Li,Yi-Hua An,Han Zhang,Hua Huang,Zhen-Qiang Liu,Yan-Bing Li,Hao Yu 한국분자세포생물학회 2013 Molecules and cells Vol.35 No.5

Artificial extracellular matrices play important roles in the regulation of stem cell behavior. To generate materials for tissue engineering, active functional groups, such as amino, carboxyl, and hydroxyl, are often introduced to change the properties of the biomaterial surface. In this study, we chemically modified coverslips to create sur-faces with different amino densities and investigated the adhesion, migration, and differentiation of neural stem cells (NSCs) under serum-free culture conditions. We observed that a higher amino density significantly promoted NSCs attach-ment, enhanced neuronal differentiation and promoted excitatory synapse formation in vitro. These results indicate that the amino density significantly affected the biological behavior of NSCs. Thus, the density and impact of functional groups in extracellular matrices should be considered in the research and development of materials for tissue engineering.

Clinical Significance of Upregulation of mir-196a-5p in Gastric Cancer and Enriched KEGG Pathway Analysis of Target Genes

Li, Hai-Long,Xie, Shou-Pin,Yang, Ya-Li,Cheng, Ying-Xia,Zhang, Ying,Wang, Jing,Wang, Yong,Liu, Da-Long,Chen, Zhao-Feng,Zhou, Yong-Ning,Wu, Hong-Yan Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.5

Background: miRNAs are relatively recently discovered cancer biomarkers which have important implications for cancer early diagnosis, treatment and estimation of prognosis. Here we focussed on expression of mir-196a-5p in gastric cancer tissues and cell lines so as to analyse its significance for clinicopathologic characteristics and generate enriched KEGG pathways clustered by target genes for exploring its potential roles as a biomarker in gastric cancer. Materials and Methods: The expression of mir-196a-5p in poorly, moderate and well differentiated gastric cancer cell lines compared with GES-1 was detected by RT-qPCR, and the expression of mir-196a-5p in gastric cancer tissues comparing with adjacent non cancer tissues of 58 cases were also assessed by RT-qPCR. Subsequently, an analysis of clinical significance of mir-196a-5p in gastric cancer and enriched KEGG pathways was executed based on the miRWalk prediction database combined with bioinformatics tools DAVID 6.7 and Mirfocus 3.0. Results: RT-qPCR showed that mir-196a-5p was up-regulated in 6 poorly and moderate differentiated gastric cancer cell lines SGC-7901, MKN-45, MKN-28, MGC-803, BGC-823, HGC-27 compared with GES-1, but down-regulated in the highly differentiated gastric cancer cell line AGS. Clinical data indicated mir-196a-5p to beup-regulated in gastric cancer tissues (47/58). Overexpression of mir-196a-5p was associated with more extensive degree of lymph node metastasis and clinical stage (P < 0.05; x2 test). Enriched KEGG pathway analyses of predicted and validated targets in miRWalk combined with DAVID 6.7 and Mirfocus 3.0 showed that the targeted genes regulated by mir-196a-5p were involved in malignancy associated biology. Conclusions: Overexpression of mir-196a-5p is associated with lymph node metastasis and clinical stage, and enriched KEGG pathway analyses showed that targeted genes regulated by mir-196a-5p may contribute to tumorgenesis, suggesting roles as an oncogenic miRNA biomarker in gastric cancer.

Linear Extended State Observer Based Sliding Mode Disturbance Decoupling Control for Nonlinear Multivariable Systems with Uncertainty

Hai-Long Xing,Dong-Hai Li,Juan Li,Cai-Hong Zhang 제어·로봇·시스템학회 2016 International Journal of Control, Automation, and Vol.14 No.4

In this paper, the sliding mode dynamic disturbance decoupling tracking control method based on thelinear extended state observer (LESO) is proposed for a class of square multivariable nonlinear uncertain system. The model plant contains the known linear dynamics, the unknown nonlinear dynamics and the internal and externaldisturbances, and the various input-output pairs are interacted. The system states are not available for measurement. An improved LESO is developed. The leading feature which is different from the typical ESO lies in that itsextended state does not contain the known linear dynamics. The improved LESO can guarantee the error variablesto be uniformly ultimately bounded with respect to a ball whose radius is a function of design parameters. So thisball radius can be arbitrarily as small as desired by tuning design parameters. And we give a simple method by whichthe gain parameters of LESO can be computed easily. This estimation to the total disturbance of the original systemis introduced into the sliding mode control design to complete disturbance rejection and decoupling. Rigorousstability analysis shows that the system output can track the desired signal closely. Finally, a class of mass–spring–damper system is taken to make the numerical simulation analysis to illustrate the effectiveness of the proposedcontrol method.

Development and Characterization of a Novel Anti-idiotypic Monoclonal Antibody to Growth Hormone, Which Can Mimic Physiological Functions of Growth Hormone in Primary Porcine Hepatocytes

Hai-Nan Lan,Hai-Long Jiang,Wei Li,Tian-Cheng Wu,Pan Hong,Yu Meng Li,Hui Zhang,Huan-Zhong Cui,Xin Zheng 아세아·태평양축산학회 2015 Animal Bioscience Vol.28 No.4

B-32 is one of a panel of monoclonal anti-idiotypic antibodies to growth hormone (GH) that we developed. To characterize and identify its potential role as a novel growth hormone receptor (GHR) agonist, we determined that B-32 behaved as a typical Ab2β based on a series of enzyme-linked immunosorbent assay assays. The results of fluorescence-activated cell sorting, indirect immunofluorescence and competitive receptor binding assays demonstrated that B-32 specifically binds to the GHR expressed on target cells. Next, we examined the resulting signal transduction pathways triggered by this antibody in primary porcine hepatocytes. We found that B-32 can activate the GHR and Janus kinase (2)/signal transducers and activators of transcription (JAK2/STAT5) signalling pathways. The phosphorylation kinetics of JAK2/STAT5 induced by either GH or B-32 were analysed in dose-response and time course experiments. In addition, B32 could also stimulate porcine hepatocytes to secrete insulin-like growth factors-1. Our work indicates that a monoclonal anti-idiotypic antibody to GH (B-32) can serve as a GHR agonist or GH mimic and has application potential in domestic animal (pig) production.

The Analysis of the Film Cooperation Mode of “One text, two productions” in China and South Korea - Take Extreme Job and Lobster cop as Examples -

Hai-Long Li(리하이롱) 한국엔터테인먼트산업학회 2019 한국엔터테인먼트산업학회논문지 Vol.13 No.8

영화분야에서 중국과 한국의 지속적인 협력에 따라, 협력의 방법도 다양한 양상을 보이고 있다.‘하나의 시나리오로 두개를 찍는’라는 말처럼 두 나라 영화제작자들이 영화시나리오를 공유하고 같은 시나리오를 활용하여 영화를 제작하는 과정이라고 이해할 수 있다. 한국의 〈극한직업〉과 중국의 〈랍스타 캅〉이라는 영화가 이러한 맥락에서 제작된 영화다. 이 극본은 2015년“중한시나리오공통개발프로젝트”에서 나왔으며,“일원다용 문화 공모전”에서 수상하였다. 영화는 한국과 중국의 영화제작자들이 촬영하였고, 양국의 제작자들의 서로 다른 문화적 배경과 미학적 관점에서 시나리오에 대하여 다양한 측면으로 수정보완 하였다. 현재, 두 영화는 한국과 중국에서 개봉하였고 높은 흥행을 찍었다.‘하나의 시나리오로 두 개를 찍는’같은 협력방식은 영화의 세계화발전에 긍정적인 영향을 가져다주었고 새로운 협력모델을 제시하였다. 본 연구는 두 영화를 연구 대상으로‘하나의 시나리오로 두개를 찍는’의 특징과 우세에 대하여 분석하고 설명하고자 한다. With the deepening cooperation between China and South Korea in the field of film, the ways of cooperation between China and South Korea are becoming more and more diversified. "One text, two productions" film cooperation mode refers to the filmmakers of two countries sharing script resources, using the same script to create films separately. South Korean Extreme Job(극한직업)and Chinese Lobster cop(龙虾刑警) are the products of this cooperation model. The scripts of the two films originated from the China and South Korea story joint development plan(중한시나리오공통개발프로젝트)in 2015. After winning the award, the script were filmed by Korean and Chinese filmmakers. Filmmakers in two countries adapted the script to different degrees according to their respective cultural backgrounds and aesthetic characteristics. The film cooperation mode of "One text, two productions" promotes the development of transnational cooperation and opens up a new way of film cooperation.

Heparanase mRNA and Protein Expression Correlates with Clinicopathologic Features of Gastric Cancer Patients: a Meta-analysis

Li, Hai-Long,Gu, Jing,Wu, Jian-Jun,Ma, Chun-Lin,Yang, Ya-Li,Wang, Hu-Ping,Wang, Jing,Wang, Yong,Chen, Che,Wu, Hong-Yan Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.18

Background: Heparanase is believed to be involved in gastric carcinogenesis. However, the clinicopathologic features of gastric cancer with high heparanase expression remain unclear. Aim : The purpose of this study was to comprehensively and quantitatively summarize available evidence for the use of heparanase mRNA and protein expression to evaluate the clinicopathological associations in gastric cancer in Asian patients by meta-analysis. Materials and Methods: Relevant articles listed in MEDLINE, CNKI and the Cochrane Library databases up to MARCH 2015 were searched by use of several keywords in electronic databases. A meta-analysis was performed to clarify the impact of heparanase mRNA and protein on clinicopathological parameters in gastric cancer. Combined ORs with 95%CIs were calculated by Revman 5.0, and publication bias testing was performed by stata12.0. Results: A total of 27 studies which included 3,891 gastric cancer patients were combined in the final analysis. When stratifying the studies by the pathological variables of heparanase mRNA expression, the depth of invasion (633 patients) (OR=4.96; 95% CI=2.38-1.37; P<0.0001), lymph node metastasis (639 patients) (OR=6.22; 95%CI=2.70-14.34, P<0.0001), and lymph node metastasis (383 patients) (OR=6.85; 95% CI=2.04-23.04; P=0.002) were all significant. When stratifying the studies by the pathological variables of heparanase protein expression, this was the case for depth of invasion (1250 patients) (OR=2.76; 95% CI=1.52-5.03; P=0.0009), lymph node metastasis (1178 patients) (OR=4.79 ; 95% CI=3.37-6.80, P<0.00001), tumor size (727 patients) (OR=2.06 ; 95% CI=1.31-3.23; P=0.002) (OR=2.61; 95% CI=2.09-3.27; P=0.000), and TNM stage (1233 patients) (OR=6.85; 95% CI=2.04-23.04; P=0.002). Egger's tests suggested publication bias for depth of invasion, lymph node metastasis, lymph node metastasis and tumor size of heparanase mRNA and protein expression. Conclusions: This meta-analysis suggests that higher heparanase expression in gastric cancer is associated with clinicopathologic features of depth of invasion, lymph node metastasis and TNM stage at mRNA and protein levels, and of tumor size only at the protein level. Egger's tests suggested publication bias for these clinicopathologic features of heparanase mRNA and protein expression, and which may be caused by shortage of relevant studies. As a result, although abundant reports showed heparanase may be associated with clinicopathologic features in gastric cancer, this meta-analysis indicates that more strict studies were needed to evaluate its clinicopathologic significance.

Removal of NO with the hexamminecobalt(II) solution catalyzed by the activated carbon treated with acetic acid

Xiang-li Long,Bei-bei Duan,Hai-xia Cao,Ming-lei Jia,Long-an Wu 한국공업화학회 2018 Journal of Industrial and Engineering Chemistry Vol.62 No.-

The simultaneous scrubbing of NO and SO2 can be finished with the Co(NH3)62+ ammonia solution. Activated carbon aids the regeneration of Co(NH3)62+ to retain the ability of absorbing NO. Acetic acid is tried to improve the catalytic capability of activated carbon. The best treatment condition is the carbon samples impregnated in 2.0 mol l−1 HAc solution for 20 h followed by being calcined at 600 °C for 4 h. The HAc modification increases surface area and acidic groups on the carbon surface. The experiments prove that the modified carbon can obtain a higher NO removal efficiency than the original carbon.

플래시 변환 계층에서 시간적 지역성을 이용하여 쓰기 요청을 처리하는 효율적인 페이지 레벨 매핑 알고리듬

이해룡(Hai-Long Li),황선영(Sun-Young Hwang) 한국통신학회 2016 韓國通信學會論文誌 Vol.41 No.10