Intracellular Localization and Sustained Prodrug Cell Killing Activity of TAT-HSVTK Fusion Protein in Hepatocelullar Carcinoma Cells

Limin Cao,Jin Si,Weiyu Wang,Xiaorong Zhao,Xiaomei Yuan,Huifen Zhu,Xiaolong Wu,Jianzhong Zhu,Guanxin Shen 한국분자세포생물학회 2006 Molecules and cells Vol.21 No.1

Gene therapy with nonviral vectors using the suicide gene/prodrug activating system of herpes simplex virus type-1 thymidine kinase (HSV1-TK)/ganciclovir (GCV) is inefficient in killing malignant tumor cells due to two major factors: (a) an unsatisfactory bystander effect; (b) short-lived expression of the protein. To study the capacity of the protein transduction domain (PTD) of HIV-1 TAT protein to enhance HSV1-TK/GCV cancer gene therapy, we constructed three fusion proteins TAT-TK, TK-TAT and TK. TATTK retained as much enzyme activity as TK, whereas that of TK-TAT was much lower. TAT-TK can enter HepG2 cells and much of it is translocated to the nucleus. The transduced HepG2 cells are killed by exogenously added GCV and have bystander effects on untransduced HepG2 cells. Most importantly, the introduced recombinant protein is stable and remains functional for several days at least, probably because nuclear localization protects it from the cytoplasmic degradation machinery and provides access to the nuclear transcription machinery. Our results indicate that TAT fusion proteins traffic intercellularly and have enhanced stability and prodrug cell killing activity. We conclude that TAT has potential for enhancing enzyme prodrug treatment of liver cancers.

A design of transmission-type multi-target X-ray tube based on electric field modulation

Zhao, Lei,Jia, Wenbao,Jin, Limin,Shan, Qing,Cheng, Can,Zhu, Hongkui,Hei, Daqian Korean Nuclear Society 2021 Nuclear Engineering and Technology Vol.53 No.9

Multi-target X-ray tube is a new type X-ray source, and can be applied in many fields such as sensitive X-ray fluorescence analysis and medical imaging. In this work, we report an electric field modulation multi-target X-ray tube, which contains four targets (Cr, Ni, Au, Mo) coated on a Beryllium (Be) window. A four-valve electric field deflector was developed to deflect the electron beam to bombard the corresponding targets. Particle dynamics analysis software was employed to simulate the particle tracking of electron beam. The results show that the 30 keV electron beam could get a 6.7 mm displacement on the target plane by 10⁵ V/m electric field. The focus areas are about 2 mm × 5 mm and 4 mm × 2.5 mm after deflection in two directions. Thermal behavior calculated by ANSYS shows that the designed target assembly could withstand a 10 W continuous power. The optimum target thicknesses and emission spectra were obtained by Geant4 when the thickness of Be window was 300 mm and the electron beam incident angle was 0.141 rad. The results indicate that this multi-target X-ray tube could provide different X-ray sources effectively.

PREPARATION AND PHOTOCHROMISM OF SINGLE AG-TCNQ MICROSTRUCTURE

Guanying Cao,Limin Ma,Xiuyan Zhao,Xin Zhao,Xueheng Tao 한국멀티미디어학회 2006 한국멀티미디어학회 국제학술대회 Vol.2006 No.-

The micro/nanostructures of metal-organic complex Ag-TCNQ were successfully synthesized via the reaction between Ag film and TCNQ dissolved in acetonitrile solution. X-ray diffraction and scanning electron microscopy confirmed that the as-obtained Ag-TCNQ was crystalline one-dimensional micro/nanowires or tubes. The reversible photochromic property of single Ag-TCNQ microstructure was investigated both by home-made optical testing system and Raman

A SIMPLELAGRANGIAL SINGLE-DOPPLER VELOCITY RETRIEVAL TECHNIQUE

Alan Shapiro,Limin Zhao,Yidi Liu 한국기상학회 1997 한국기상학회 학술대회 논문집 Vol.1997 No.-

Enhancement of Kidney Invigorating Function in Mouse Model by Cistanches Herba Dried Rapidly at a Medium High Temperature

Yehong Guo,Limin Wang,Qian Li,Cheng Zhao,Ping He,Xudong Ma 한국식품영양과학회 2019 Journal of medicinal food Vol.22 No.12

Cistanches Herba is a popular Traditional Chinese Medicine and functional food that is used to treat kidney yang deficiency (KYD) in China. In this study, we investigated the effects of different drying methods for Cistanches Herba on kidney invigoration and yang strengthening. We established a mouse model of KYD by intraperitoneal injection of hydrocortisone for 8 days. We dried slices of Cistanches Herba in the sun, in the shade, in a microwave, or in an oven at 40°C, 60°C, 80°C or 100°C, then prepared and administered extracts to the mice by gastric gavage. We measured and evaluated the echinacoside (ECH) and acteoside (ACT) contents of the extracts, as well as the mice's body weight; testicular, epididymal, hepatic, and renal coefficients; and semen quality. All the Cistanches Herba extracts, obtained using different drying techniques, improved symptoms of KYD diagnosis in mice. Among them, treatments with Cistanches Herba dried in a microwave and dried in an oven at 100°C had the best therapeutic effects. Our results suggested that the higher the total content of ECH and ACT in Cistanches Herba extracts, the better the effects of kidney invigoration and yang strengthening. In addition, shorter drying times at higher temperatures lead to the highest recoveries of active components, and Cistanche dried in the sun at a medium-high temperature can improve sperm quality in mice.

Effects of Acute Sacral Neuromodulation at Different Pulse Widths on Bladder Overactivity in Pigs

Huiling Cong,Limin Liao,Yiming Wang,Lingna Zhao,Zhaoxia Wang,Guang Fu,Guoqing Chen 대한배뇨장애요실금학회 2019 International Neurourology Journal Vol.23 No.2

Purpose: Sacral nerve stimulation has been used to treat overactive bladder. This study evaluated the effects of stimulation using different pulse widths on the inhibition of bladder overactivity by sacral nerve stimulation (SNM) in pigs. Methods: Implant-driven stimulators were used to stimulate the S3 spinal nerve in 7 pigs. Cystometry was performed by infusing normal saline (NS) or acetic acid (AA). SNM at pulse widths of 64 μsec to 624 μsec was conducted at the intensity threshold at which observable perianal and/or tail movement was induced. Multiple cystometrograms were performed to determine the effects of different pulse widths on the micturition reflex. Results: AA-induced bladder overactivity reduced the bladder capacity to 46.9%±7.1% of the NS control level (P<0.05). During AA infusion, SNM at 64 μsec, 204 μsec, and 624 μsec increased the bladder capacity to 126.1%±6.9%, 129.5%±7.3%, and 140.1%±7.6% of the AA control level (P<0.05). No significant differences were found among the results obtained using pulse widths of 64 μsec, 204 μsec, and 624 μsec (P>0.05). The actual intensity threshold varied from 0.7 to 8 V. The mean intensity threshold (T visual) for pulse widths of 64 μs, 204 μs, and 624 μs were 5.64±0.76 V, 3.11±0.48 V, and 2.52±0.49 V. T visual for pulse widths of 64 μsec was larger than the other two T visual for pulse widths of 204 μsec and 624 μsec (P<0.05). No significant differences were found among the T visual for pulse widths of 204 μsec and 624 μsec (P>0.05). Conclusions: This study indicated that different pulse widths could play a role in inhibiting bladder overactivity. It is not yet certain which pulse widths increased bladder capacity compared with AA levels, to minimize energy consumption and maintain patient comfort during stimulation, 204 μsec may be an appropriate pulse width for SNM.

Framework Design of Emergency Management Information System for Cloud Computing in Chemical Park

Jinfeng Zhang,Xin Zhao,Xin Liu,Limin Hou 보안공학연구지원센터 2014 International Journal of Future Generation Communi Vol.7 No.5

Oxygen removal from nitrogen using YBaCo_2O_(5+δ) adsorbent

Haoshan Hao,Bojun Chen,Limin Zhao,Xing Hu 한국화학공학회 2011 Korean Journal of Chemical Engineering Vol.28 No.2

The oxygen intake/release behavior of YBaCo_2O_(5+δ) and its application as a medium-temperature (100-600℃) adsorbent to oxygen removal in nitrogen purification was investigated. The oxygen content in YBaCo_2O_(5+δ)was varied between 5.46 and 5.0 with the change of the temperature and oxygen partial pressure. This oxygen nonstoichiometry showed good reversibility and the crystal structure of YBaCo_2O_(5+δ) remained stable in the oxygen intake/release process. Results of nitrogen purification experiment show that YBaCo_2O_(5+δ) material is a promising candidate to remove trace oxygen from nitrogen. After being deoxidized at 600 oC in nitrogen, 1 kg YBaCo_2O_(5+δ) adsorbent can purify 310 L nitrogen of 98.1% to high-purity nitrogen of over 99.9999% in one cycle.

The Expression and Relationship of CD68-Tumor-Associated Macrophages and Microvascular Density With the Prognosis of Patients With Laryngeal Squamous Cell Carcinoma

Shujun Sun,Xinliang Pan,Limin Zhao,Jianming Zhou,Hongzeng Wang,Yonghong Sun 대한이비인후과학회 2016 Clinical and Experimental Otorhinolaryngology Vol.9 No.3

Objectives. We sought to identify the expression of CD68-tumor-associated macrophages (TAMs) and CD34-microvascular density (MVD) in laryngeal squamous cell carcinoma (LSCC), to study the relationship with clinical pathological parameters and to determine whether their expression is predictive of disease. Methods. Pathologically confirmed 45 LSCC tissue and 20 peritumoral non-tumor tissue were examined. Immunohistochemical studies were used to detect the expression of CD68-TAMs and CD34-MVD. Results. The positive expression rate of CD68 in LSCC tissue was 82% (37/45), which was higher than the 10% (2/20) expression rate of the peritumoral tissue (P<0.05). The CD34-MVD positive expression rate in the LSCC tissue was 26.5±6.4, which obviously higher than 12.2±4.0 expression rate of the peritumoral tissue (P<0.05). The positive expression rates of both CD68 and CD34-MVD were higher in the lymph node metastasis (LNM) positive group than in the LNM negative group. The expression of CD68 had positive correlation with CD34-MVD. The 5-year disease-free survival rate in the group with the low CD68 expression was significantly higher than that in the group with high CD68 expression (76% vs. 42%, respectively). Conclusion. The high expression of CD68-TAMs in LSCC and its positive correlation with CD34-MVD illustrates that both play an important role in promoting the metastasis and angiogenesis of this cancer. Their expression was also positively correlated with the prognoses of these patients, suggesting that they could be used as important prognostic markers for LSCC.

Structure and magnetoelectric property of low-temperature sintering (Ni_0.8Zn_0.1Cu_0.1)Fe_2O_4/[0.58PNN-0.02PZN-0.05PNW-0.35PT] composites

Renbing Sun,Bijun Fang,Limin Zhou,Qinhui Zhang,Xiangyong Zhao,Haosu Luo 한국물리학회 2011 Current Applied Physics Vol.11 No.1

(1-x)(Ni_0.8Zn_0.1Cu_0.1)Fe_2O_4/x0.58Pb(Ni_1/3Nb_2/3)O_3-0.02Pb(Zn_1/3Nb_2/3)O_3-0.05Pb(Ni_1/2W_1/2)O_3-0.35PbTiO_3((1-x)NZCF/xPNZNWT) magnetoelectric composites were prepared by conventional solidestate reaction method via low-temperature sintering technique. The low-temperature sintering technique can prepare dense composites, where the ferrite and piezoelectric phases coexist in the synthesized particulate composites, and no apparent mutual solubility takes place between the two phases. The (1-x)NZCF/xPNZNWT ceramic composites exhibit excellent integrated electrical properties, where diffused dielectric response peaks, typical P-E hysteresis loops and excellent piezoelectric property can be obtained. The (1-x)NZCF/xPNZNWT composites prepared by the low-temperature sintering process can be electrically and magnetically poled to exhibit apparent magnetoelectric effect, indicating that such technique is convenient to develop excellent particulate magnetoelectric materials in the future.