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혈액 투석 환자에서 중심정맥 협착에 대한 스텐트 삽입술 : Wallstent Placement
임대승,노상필,이유선,정승현,김보영,이정우,강정아,김정희,이민수,정준용,최시완,정진옥,성인환,이강욱,신영태 충남대학교 의과대학 의학연구소 2002 충남의대잡지 Vol.29 No.1
Stenosis of central vein is a common complication arising after percutaneous subclavian vein catheter insertion performed for temporary vascular access in chronic renal failure patients undergoing hemodialysis. There are several treatment methods for the condition like percutaneous angioplasty(PTA), stent insertion, and surgery, but recent trend is toward PTA and stents. Among the patients diagnosed with chronic renal failure from March 1993 to May 2002 and undergoing hemodialysis through AV fistula, the 14 Patients in whom central vein stenosis arose were selected for the study. A total of 28 percutaneous interventions(5 PTA and 23 stent placement) were performed, and restenosis rate and the time taken till the restenosis in de novo lesions and instant lesions were compared. All 28 cases were operated successfully. The 14 cases that received both anigioplasty and stent placement initially. (de novo lesion : 14 cases), Among the 10 cases with de novo lesion that followed up more than 1 year, 3 cases are currently undergoing hemodialysis without restenosis, and the remaining 7 cases have recurred stenosis with the mean time to restenosis of 10.9 months. In the 7 cases in whom stenosis recurred, 11 interventions were done(instent lesion: 11 cases). 4 of these were using only ballon angioplasty with 100% restenosis rate and the mean time of 3 months until restenosis. The remaining 7 cases were using both balloon angioplasty and stent placement, also with 100% restenosis rate but with the mean time of 12 months until restenosis, which was later than the group receiving only balloon angioplasty. In treating the patients with central vein stenosis, stent placement seems to be more advantageous over PTA in terms of restenosis rate and the mean duration of patency. In the case of instent lesion, inserting the stent for the second time after stenosis recurred lengthened the duration of patency compared to performing balloon angioplasty alone.
원거리 플라즈마 화학증착법으로 증착된 이산화규소박막의 물성
박영배,강진규,이시우,Park, Yeong-Bae,Gang, Jin-Gyu,Lee, Si-U 한국재료학회 1995 한국재료학회지 Vol.5 No.6
원거리 플라즈마 화학증착법을 이용하여 저온에서 이산화규소박막을 제조하였다. 본 연구 에서는 공정변수인 기판의 온도, 반응기체의 조성 및 분압과 플라즈마 전력에 따른 산화막의 재료적인 물성을 평가하였다. XPS결과에서 산화막은 양론비(O/Si=2)보다 약간 적어 실리콘이 많이 함유된 막으로 나타났다. 이 경우 굴절율과 ESR분석에 의해 미결합된 실리콘의 양이 증가함을 알 수 있었다. SIMS분석에 의해 미량의 질소성분이 계면에 존재하는 것과 실리콘 미결함을 관찰하였다. FT-IR로부터 막내 수소량을 정량화하였으며 결합각 분포는 20$0^{\circ}C$이상에서 열산화막과 비슷한 값을 얻었다. 하지만 열산화막에 비해 높은 식각율을 보여 계면 스트레스에 의해 막내의 결합력이 약해진 것으로 생각된다. Silicon oxide thin films were deposited by remote plasma chemical vapor deposition (RPCYD). The effect of the operating variables, such as plasma power, deposition temperature and partial pressure of reactant on the material Properties of the silicon oxide film was investigated. By XPS, it was found out that the film was suboxide (O/Si<2) and small amount of nitrogen due to the plasma excitation was accumulated at the Si/SiO$_2$interface. The amount of dangling bonds at the Si/SiO$_2$interfaces were measured by ESR and the concentration of hydrogen bond was obtained by SIMS and FT-IR. The bond angle distribution(d$\theta$/$\theta$) was shown to be similiar to thermal oxide above 20$0^{\circ}C$ but the etch rate was higher than that of the thermal oxides due to the structural difference and the stress between silicon substrate and silicon oxide film.
지방산, 지방 알코올 및 프로필렌글리콜이 클렌부테롤의 경피투과에 미치는 영향
이영대,권기철,정시영,이종달,용철순 영남대학교 약품개발연구소 2000 영남대학교 약품개발연구소 연구업적집 Vol.10 No.-
Clenbuterol, a selective β_(2)-adrenergic receptor stimulant, has been introduced as a potent bronchodilator for patients with bronchial asthma, chronic bronchitis and pulmonary emphysema. For the purpose of developing a transdermal preparation for clenbuterol, we attempted to select an optimal solvent system and permeation enhancer among fatty acids and fatty alcohols which are known to accelerate the penetration of various drugs in permeation experiments using hairless mouse skin and Franz diffusion cell. Apparent partition coefficient of clenbuterol was increased as pH of buffer solution was increased and solubility of clenbuterol was increased as the percent of propylene glycol(PG) in buffer solution(pH10) was increased. Permeability of clenbuterol from different buffer(pH 10)/PG solvent mixtures was decreased as the percent of PG in pH 10 buffer solution was increased and among the various enhancers studied, lauryl alcohol was found to be the most effective enhancer, increasing the permeability of clenbuterol approximately 76-fold compared with control. Lauryl alcobol(0-2%) enhanced the permeability of clenbuterol concentration-dependently. In this study, the optimal solvent system for the penetration of clenbuterol was found to be 50/50 buffer(pH 10)/PG solvent mixture containing 2% lauryl alcohol.
PCR과 SDS-PAGE를 이용한 Bifidobacteria의 동정
박시홍,송희성,이지연,김해영 경희대학교식량자원개발연구소 2005 硏究論文集 Vol.24 No.-
SDS-PAGE를 이용한 단백질 패턴의 분석결과, 같은 종의 Bifidobacterium은 동일한 단백질 패턴을 보이며, 다른 종의 Bifidobacterium은 서로 다른 패턴을 보이고 있는 것을 확인하였다. 따라서 SDS-PAGE를 이용한 whole cell protein 패턴 분석으로 Bifidobacterium을 간편하고 빠르게 동정할 수 있는 가능성을 보였다. DNA homology 98% 이상으로 PCR에 의해 동정에 어려움이 많았던 B. catenulatum, B. Pseudocatenulatum group, B. animalis을 SDS-PAGE로 확인한 결과, B. catenulatum과 B. pseudocatenulatum, B. animalis는 뚜렷한 단백질 패턴의 차이를 보임으로써 구별이 가능했다. PCR 수행 시 primer제작은 기존에 보고된 것과 NCBI blast program을 통하여 제작을 하였으며, 그 결과 B. longum, B. bifidum, B. adolescentis 3종류의 종에서만 특이적으로 band가 나타나는 것을 확인할 수가 있었다. 본 실험에서는 SDS-PAGE와 PCR을 이용한 균주 동정법으로 신속하고 정확하게 Bifidobacterium을 탐색할 수 있었으며, 발효유 제조나 의약품 제조 등 산업적으로 이용 될 수 있을 것이다 (Mitsuoka, 1984). Bifidobacterium is a Gram-positive obligate anaerobic bacterium which is able to change cellular shape by nutritional conditions and dominant species in intestinal microflora of human. In order to identify Bifidobacteria spp. in human intestine, PCR and SDS-PAGE of whole cell proteins were used. Species-specific primer sets based on the 16S rRNA, 16S-23S intergenic spacer and heat shock protein genes of three Bifidobacteria spp. were constructed using NCBI blast program (Bifidobacteria adolescentis, B. longum and B. bifidum). PCR products using each primer sets showed the same sizes of expected target genes. From the SDS-PAGE of whole cell proteins in bifidobacteria, profiles of SDS-PAGE between 13 type strains showed distinct differences. These methods will be useful for monitoring and evaluating Bifidobacteria spp. in human intestine microbial population.
안성환,황동원,최준영,송시준,전용우,박원주,이광식 영남대학교 공업기술연구소 2001 工業技術硏究所論文集 Vol.29 No.1
This paper describes the development of sputtered tungsten electrode for discharge tube by making use of Sputtering method using plasma. The Xe discharge tube was designed and fabricated by using sputtered tungsten electrode, and the ICCD(Intensified Charged Coupled Device) and monochromator were used in order to observe the discharge image distribution and to measure the signal intensity. As a result, the emission light form the electrode was spreaded almost uniformly over a wide wavelength band, and the discharge image distribution and the signal intensity show quite a uniform distribution. The emission light from the Xe discharge tube was peaked at 488[nm] and 648[nm].
악성 담도폐쇄 환자에서 막부착형 금속배액관과 막비부착형 금속배액관의 비교
이상호 ( Lee Sang Ho ),차상우 ( Cha Sang U ),천영국 ( Cheon Yeong Gug ),김영석 ( Kim Yeong Seog ),문종호 ( Mun Jong Ho ),조영덕 ( Jo Yeong Deog ),김연수 ( Kim Yeon Su ),이준성 ( Lee Jun Seong ),이문성 ( Lee Mun Seong ),심찬섭 ( Si 대한소화기학회 2003 대한소화기학회 추계학술대회 Vol.2003 No.-
<목적> 수술적 치료가 불가능한 악성 폐쇄성 황달 환자에서 기존의 플라스틱 배액관의 단점을 보완하기 위해 보다 넓은 내경을 확보할 수 있는 자가팽창형 금속배액관이 개발되어 효용성이 인정되고 있다. 금속 배액관의 종양내 증식을 방지하기 위해 막부착형 배액관이 개발되었으나 막비부착형 금속 배액관과 비교하여 배액관 기능유지의 정도나 합병증 발생정도는 논란의 여지가 많은 실정이다. 이에 연자 등은 막부착형 금속배액관과 막비부착형 금속배액관의 기능유지 및 합병증
Left pneumonectomy using self-cutting linear endoscopic stapler in a dog
이시은(Si Eun Lee),박효진(Hyojin Park),이채영(Chae-Yeong Lee),최현종(Hyeon-Jong Choi),김효연(Hyoyeon Kim),김민연(Minyeon Kim),홍정호(Jeongho Hong),유도현(DoHyeon Yu),황태성(Tae Sung Hwang),이희천(Hee Chun Lee),이재훈(Jae-Hoon Lee),이동 한국예방수의학회 2021 예방수의학회지 Vol.45 No.4
A 13-year-old mixed dog was referred to the animal medical center, Gyeongsang National University. Lung masses were diagnosed at the left cranial and caudal lobes through diagnostic imaging, and consequently, left pneumonectomy was performed using a self-cutting linear endoscopic stapler. The pulmonary arteries, veins, and bronchus of each lung lobe were sealed and resected at once, and any air leakage or bleeding was not observed after the surgery. Compared to the conventional ligation method, the self-cutting linear endoscopic stapler has the advantage of significantly reducing the operation time and enabling simple and reliable sealing.