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- 저자 : Lee Jue-Hee (검색결과 78 건)
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청열해독용 한약재의 항진균작용에 의한 항감염성 관절염 효과검색
공수현,이주영,박지혜,김송이,이주희,한용문 동덕여자대학교 종합약학연구소 2007 동덕약학연구지 Vol.11 No.-
In present study, we tested antifungal effects of 11 different kinds of compounds isolated from Phellodendri Cortex (PC), Lonicerae Flos (LF), Lonicerae Folium et Caulis (LFC) and Araliae Continental is Radix (ACR). These herbal medicines are generally applied for the treatment of inflammations. Prior to the test, antifungal effect of 70%-ethanol extracts of the medicines was examined. Results showed that the three extracts of PC, LF and LFC, but not ACR extract, inhibited Candida albicans growth, in-vitro. Among the 11 compounds, berberine and palmatine isolated from PC showed the most antifungal activity (P < 0.05). Lonicerin and loganin from LF and chlorogenic acid from LFC also retarded growth of C. albicans at high dose of 250 ㎛/ml, but their activity was much less than berberine or palmatine. However, ent-Pimara-8(14),15-diene-19-oic acid and 16α,17- Dihydroxy-ent-kauran-19-oic acid from ACR had no antifungal activity as expected. All together, these data imply that most of oriental herbal medicines having anti-inflammatory activity could have anticandidal effect, suggesting that PC, LF and LFC be effective for the treatment of septic arthritis by killing of C. albicans.
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Chlorogenic acid Rutin의 Macrophage 및 T-임파구 증식에 대한 효과
박지혜,공수현,이주영,김송이,이주희,한용문 동덕여자대학교 종합약학연구소 2007 동덕약학연구지 Vol.11 No.-
In the present study, we examined immunoregulatory activitres of cholrogenic acid (CA) and rutin that are isolated from Lonicera Flos (A family of Caprifoliaceae). For the examination, NO production of LPS-treated RAW264.7 monocyte/macrophage cell line and effect on the T-cells proliferation were determined. Results showed that CA inhibited NO production at low concentrations below 10 ㎛/ml, but at higher concentration ranged from 10 ㎛/ml to 40 ㎛/ml the inhibitory activity was lost. However, rutin blocked the NO production in a dose-dependancy. In case of T-cell proliferation, both of the compounds suppressed the proliferation (P < 0.05). Combined together, it is assumed that the compounds might be effective to arthritic inflammation, possibly, by removal of CD4+ T-cells, but rutin might be more effective against the inflammation because of the blockage of NO production from macro phages which is responsible for the pain during the process of arthritis.
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Candida albicans Can Utilize Siderophore during Candidastasis Caused by Apotransferrin
Lee Jue-Hee,Han Yong-Moon The Pharmaceutical Society of Korea 2006 Archives of Pharmacal Research Vol.29 No.3
Ability of iron acquisition of pathogenic microorganisms functions as a virulence factor. Candida albicans, a fungal pathogen that requires iron for growth, is susceptible to growth retardation by high-affinity iron binding proteins such as transferrin. Recently, we reported that C. albicans could utilize the heme as a part of heme-containing proteins dissociated by heme oxygenase, CaHMX1. In search of another pathway that C. albicans can use to bypass the growth regulation produced by iron limitation, this present study examined utilization of non-candidal siderophores such as Desferal and rhodotorulic acid (RA) for acquisition of inorganic iron by the fungus. C. albicans secreting no siderophores was cultured in iron-free (pretreated with apotransferrin for 24 h) (culture medium). Once growth of the yeast reached stasis from iron starvation, a siderophore was added to the culture media. Results showed that cultures containing apotransferrin within a dialysis membrane recovered growth to the level of untreated controls, whereas C. albicans yeast cells in direct contact with soluble iron-free (apo) transferrin recovered growth only partially. When static growth from iron limitation was reached, the addition of siderophore-apotransferrin complex to culture medium also permitted the yeast to recover growth from apotransferrin growth regulation. All the data show that C. albicans can utilize the non-candidal siderophores for iron acquisition under transferrin regulation as can pathogenic bacteria.
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Preoperative Diagnosis and Medical Treatment of Pelvic Actinomycosis
Lee, Hong-Jue,Lee, Su-Jin,Kim, Young-Jae,Kim, Sung-Hee,Lee, Jung-Han,Kim, Seung-Ryoung,Cho, Sam-Hyun 대한미생물학회 2008 Journal of Bacteriology and Virology Vol.38 No.2
The diagnosis of the pelvic actinomycosis is seldom made preoperatively because of no reliable or specific clinical manifestation which has tendency to mimic advanced gynecological malignancy and the relative infrequency of the disease. To explore the method for improvement of preoperative diagnosis and possibility of avoiding the surgical management of pelvic actinomycosis, we collected and summarized the data of age, parity, state of menopause, history of intrauterine device (IUD) use, symptoms, laboratory findings, radiologic findings, provisional diagnosis and treatment from 14 cases diagnosed pathologically and treated in Hanyang University Hospital from 2000 to 2007. Eleven (78.6%) of 14 cases were IUD users. Most common complaints were lower abdominal pain (71.4%) and vaginal discharge (57.1%) which were followed by fever (28.6%) and back pain (28.6%). Four cases (28.6%) were identified as pelvic actinomycosis before operation and in 3 cases (21.4%) malignancy was provisional preoperative diagnosis. Pelvic actinomycosis was suspected via abdominal computed tomography (CT) or cervicovaginal cytology and confirmed via endometrial biopsy or fine needle aspiration biopsy. Two cases that were diagnosed before operation and received only antibiotics therapy had no recurrence. It was suggested that pelvic actinomycosis could be suspected via abdominal CT and cervicovaginal cytology in IUD users, and endometrial biopsy and fine needle aspiration biopsy may help establish the diagnosis before the operation. Adequate preoperative antibiotics therapy could make extensive exploratory surgery avoided or conservative surgery feasible.
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Lee, Jue-Yeon,Choo, Jung-Eun,Choi, Young-Sook,Lee, Kuen-Yong,Min, Do-Sik,Pi, Sung-Hee,Seol, Yang-Jo,Lee, Seung-Jin,Jo, In-Ho,Chung, Chong-Pyoung,Park, Yoon-Jeong Wiley Publishers 2007 JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A Vol. No.
<P>Fibroblast growth factor (FGF)-2 regulates a variety of cellular functions, such as proliferation and differentiation, by binding to cell surface FGF receptors (FGFRs) in the presence of heparin proteoglycans. FGF-2 is known as a heparin-binding growth factor, but the localization of the heparin binding site has not been fully investigated until now. We used two potential heparin binding domains of FGF-2, the residues 105–111 (F105, YKRSRYT) and 119–135 (F119, KRTGQYKLGSKTGPGQK). Peptides could be stably immobilized onto the surface of tissue culture plates. Using solid phase binding assays, we demonstrated that both peptides had higher binding affinity toward heparin compared with nonbinding control sequence. The biological significance of these sites was tested by cell attachment and osteoblast differentiation studies. Cell attachment to the peptides F105 and F119 increased in a dose-dependent manner. Heparin and heparinase treatments decreased cell adhesion to both F105 and F119. This demonstrates that both F105 and F119 interact with cell-surface heparan sulfate proteoglycans, suggesting that FGF-2 has two heparin binding sites. In addition, osteoblast differentiation, confirmed by ALPase activity and mineralization, was increased by surface immobilized peptide F105 and F119. Taken together, these heparin binding peptides could be applied as biological agents enhancing osteoblast differentiation as well as surface modification tools in the tissue regeneration area, especially for bone regeneration. © 2007 Wiley Periodicals, Inc. J Biomed Mater Res, 2007</P>
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외래마취시 전처치제로 사용한 Midazolam의 마취회복 및 회복실 퇴실 시간에 미치는 영향
이봉재,이종연,박정현,이병희,양현정,김민구,사해경,길현주,정금희 대한마취과학회 1999 Korean Journal of Anesthesiology Vol.37 No.1
Background: Midazolam is often used as an anxiolytic premedication before surgery. But preoperatively administered midazolam may contribute to postopertive sedation and delayed recovery from general anesthesia. This study was undertaken to evaluate the effect of midazolam premedication on postoperative recovery and discharge-readiness after brief outpatient surgery. Methods: Sixty healthy ASA physical status I women scheduled for outpatient diagnostic laparoscopic surgery were considered for the study. They were randomly allocated to one of two groups. Group one received normal saline (N/S) 5 ml intravenously (IV), while group two received IV midazolam 0.04 mg/kg. The study drug was prepared in 5 ml of saline and administered 10 minutes before the induction of general anesthesia. General anesthesia was induced with fentanyl, propofol and vecuronium and was maintained with N2O and enflurane. Postanesthetic recovery (PAR) scores were recorded after the arrival of the patients in the postanesthetic recovery room. Sedation was quantified before and after premedication and 60, 120 minutes after arriving in the postanesthetic recovery room, using the symbol-digit-modalities test (SDMT) and trail-making test (TMT). Results: There were no significant differences between the two groups with respect to age, weight and anesthesia time. There were no significant differences in PAR scores or PAR-stay time between two groups. SDMT and TMT scores were significantly different 5 minutes after the study's drug administration, and 60 minutes after arrival in the postanesthetic recovery room between the two groups. The incidence of side effects was similar in both groups. Conclusions: Midazolam premedication proved effective in sedation and anxiolysis without prolonging postanesthetic recovery and discharge times for outpatient general anesthesia. (Korean J Anesthesiol 1999; 37: 1∼5)
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Jue-Hee Lee 대한약학회 2011 Archives of Pharmacal Research Vol.34 No.7
Arthritis due to pathogenic fungi is a serious disease causing rapid destruction of the joint. In the pathogenesis of arthritis, T lymphocytes are considered to be one of the major immune cells. In present study, we examined the T cell immunoregulatory effect by ochnaflavone (Och), a biflavonoid, on arthritis caused by Candida albicans that is the most commonly associated with fungal arthritis. To examine the effects of ochnaflavonon Candida albicans-caused septic arthritis, an emulsified mixture of C. albicans cell wall and complete Freund's adjuvant (CACW/CFA) was injected into BALB/c mice via hind footpad route on days -3, -2, and -1. On Day 0, Och at 1 or 2 mg/dose/time was intratraperitoneally given to mice with the swollen footpad every other day for 3 times. The footpad-edema was measured for 20 days. Results revealed that Och reduced the edema at all dose levels and furthermore, there was app. 45% reduction of the edema in animals given 2 mg-dose at the peak of septic arthritis (p < 0.05). This anti-arthritic effect was accompanied by the diminishing of the DTH (delayed type hypersensitivity) activity against the CACW and by the provoking of the dominant T helper 2 (Th2) type cytokines production (IL-4 and Il-10), which appeared to result in a suppression of T helper 1 cytokines (IFN-γ and IL-2). Besides the T cell immunoregulatory activity, Och inhibited T cells activation as evidenced by the IL-2 reduction from PMA/ionomycin-stimulated Jurkat cell line and in addition, the compound killed macrophages in a dose-dependent manner (p < 0.05). However, Och caused no hemolysis (p < 0.05). These data implicate that Och, which has anti-arthritic activity based on the Th2 dominance as well as macrophage removal, can be safely administered into the blood circulation for treatment of the arthritis caused by C. albicans. Thus, it can be concluded that Och would be an ideal immunologically evaluated agent for treating of Candida arthritis.
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이주희(Jue Hee Lee),심진기(Jin Kie Shim),한용문(Young Moon Han) 대한약학회 2005 약학회지 Vol.49 No.5
Our previous data showed the protein isolated from Coptidis Rhizoma(CRP) had antifungal activity. In present study, we examined mode of action of the CRP and its activity against subcutaneous candidiasis 엳 새 C. albicans yeast cells. Results showed that the CRP blocked hyphal production from yeast form of C. albicans. The CRP also activated RAW 264.7 monocyte/macrophage cell line, which resulted in nitiric oxide (NO) production from the cells. This activation seemed to increase macrophage phagocytosis to destroy the invaders. Like other antimicrobial peptides, CRP was influenced by ionic strength, thus resulting in a decrease of antifungal activity. In murine model of a subcutaneous candidiasis, the sizes of infected areas of the nude mice given the CRP after subcutaneous injection of C. albicans yeast cells to the dorsal skin were 90% less than those of the nude mice groups that received DPBS instead of the CRP. All data indicate that the CRP, which appeared to act like an antimicrobial peptide and to inhibit the morphological transition from blastoconidia, was effective against the subcutaneous disease.
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