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Lee, Joo H.,Lee, Myung G. WILEY PUBLISHERS 2007 BIOPHARMACEUTICS AND DRUG DISPOSITION Vol.28 No.4
<P>It was reported that the expression of CYP3A1 increased in rats with acute renal failure induced by uranyl nitrate (rat model of U-ARF) compared with controls. It was shown that telithromycin was mainly metabolized via CYP3A1/2 in rats in this study. Hence, the pharmacokinetic parameters of telithromycin were compared after both intravenous and oral administration at a dose of 50 mg/kg to control rats and a rat model of U-ARF. After intravenous administration of telithromycin to rats with U-ARF, the AUC and renal clearance (Cl<SUB>r</SUB>) were significantly greater (35.0% increase) and slower (99.1% decrease), respectively, than the controls. Unexpectedly, the nonrenal clearance (Cl<SUB>nr</SUB>) of telithromycin was comparable between the two groups of rats, suggesting that CYP3A isozyme responsible for the metabolism of telithromycin seemed not to be expressed considerably in the rat model of U-ARF. After oral administration of telithromycin to rats with U-ARF, the AUC was also significantly greater (127% increase) than the controls and the value, 127%, was considerably greater than 35.0% after intravenous administration of telithromycin. This may be due mainly to the decrease in the intestinal first-pass effect of telithromycin compared with controls in addition to significantly slower Cl<SUB>r</SUB> than controls. Copyright © 2007 John Wiley & Sons, Ltd.</P>
Lee, Dongwoo,Park, Kwang‐,Sook,Yoon, Gook Jin,Lee, Hyun Jung,Lee, Jue‐,Yeon,Park, Yoon Shin,Park, Joo‐,Cheol,Lee, Gene,Chung, Chong Pyoung,Park, Yoon Jeong Wiley Publishers 2019 JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A Vol.107 No.11
<P><B>Abstract</B></P><P>Peptide and proteins are recognized as highly selective and therapeutically active biomaterials, as well as relatively safe in clinical application. A calcium phospholipid‐binding protein, copine 7 (CPNE7), has been recently identified to induce hard tissue regeneration, including bone and dentin by internalizing into the cells. However, the clinical application of the full length of CPNE7 has limited due to its large size with short half‐life. Herein, as an alternative to CPNE7, six bioactive synthetic peptides are designed from CPNE7 (CPNE7‐derived peptides, CDP1–CDP6) and investigated their osteogenic potential. Among the CDPs, CDP4 have the highest level of cell‐penetrating activity as well as osteogenic efficiency in dental pulp stem cells (DPSCs). CDP4 increased the expression of osteogenesis‐related genes and proteins, which was comparable to that by BMP‐2. The cell penetration capacity of CDP4 may synergistically induce the osteogenic potential of DPSCs. Moreover, the implantation of the mixture of CDP4 with injectable collagen gel increased bone formation with recovery in the mouse calvarial defect model, comparable to full‐length CPNE7 and even BMP‐2. In conclusion, these results suggest that our synthetic peptide, CDP4, can be applied in combination with biomaterial to provide high osteogenic efficacy in the field of bone tissue engineering.</P>
Moon, Hyun-Ju,Kim, Kyoung-Nam,Kim, Kwang-Mahn,Choi, Seong-Ho,Kim, Chong-Kwan,Kim, Kee-Deog,LeGeros, Racquel Z.,Lee, Yong-Keun Wiley Publishers 2005 Journal of Biomedical Materials Research Part A Vol. No.
<P>The purpose of this study was to investigate the bone-regenerative effect of calcium phosphate glass in vivo. We prepared amorphous calcium phosphate glass powder having a mean particle size of 400 μm in the system CaO-CaF<SUB>2</SUB>-P<SUB>2</SUB>O<SUB>5</SUB>-MgO-ZnO. Calvarial critical-sized defects (8 mm) were created in 60 male Sprague-Dawley rats. The animals were divided into an experimental group and control group of 30 animals each. Each defect was filled with a constant weight of 0.5 g calcium phosphate glass powder mixed with saline. As a control, the defect was left empty. The rats were sacrificed 2, 4, or 8 weeks postsurgery, and the results evaluated using radiodensitometric and histological studies; they were also examined histomorphometrically. When the calcium phosphate glass powders with 400-μm particles were grafted, the defects were nearly completely filled with new-formed bone in a clean healing condition after 8 weeks. It was observed that the prepared calcium phosphate glass enhanced new bone formation in the calvarial defect of Sprague-Dawley rats and could be expected to have potential for use as a hard tissue regeneration material. © 2005 Wiley Periodicals, Inc. J Biomed Mater Res, 2005</P>
Kim, Mi-Sook,Choi, Yoon-Jeong,Noh, Insup,Tae, Giyoong Wiley Publishers 2007 Journal of Biomedical Materials Research Part A Vol. No.
<P>We developed and characterized a novel in situ chitosan-poly(ethylene oxide) (PEO) hydrogel via two steps: 2-carboxyethyl acrylate molecules were grafted to the primary amine functional groups in chitosan in the first step and then Michael type addition reaction was processed between the grafted acrylate end groups and the thiol end groups in the PEO. Grafting of acrylate molecules to the amine groups in the deacetylated water soluble chitosan was confirmed by observing the new acrylate peaks by the FTIR and NMR spectra of the acrylated chitosan samples, as well as changes in relative viscosities of chitosan and acrylated chitosan. Formation of the chitosan-PEO hydrogel was visually observed with digital images after both gelation and hydration. Rheological analyses of the hydrogel formation were performed to detect its gelation time, phase angle changes, and visco-elastic properties over frequency and strain percentage. Their results indicated that the gelation process was completed within 10 min after mixing the precursor liquid solutions. An extent of water swelling, mechanical strength against compression and the morphologies of the hydrogel surface and cross sections after dehydration process were analyzed by microbalance measurement, texture analyzer, and scanning electron microscopy observation, respectively. Biological activities of the hydrogels were evaluated by observing smooth muscle cell behaviors such as cell adhesion and viability as well as by measuring the number of adhered cells on their surfaces. © 2007 Wiley Periodicals, Inc. J Biomed Mater Res 2007</P>
Microrough titanium surface affects biologic response in MG63 osteoblast-like cells
Kim, Myung-Joo,Kim, Chang-Whe,Lim, Young-Jun,Heo, Seong-Joo Wiley Publishers 2006 Journal of Biomedical Materials Research Part A Vol. No.
<P>The purpose of this study was to define the surface properties of prepared titanium (Ti) disks, which served as a model system, and to contrast the biologic response of MG63 cells exposed to Ti disks with different levels of surface roughness. The surface properties interact with each other, resulting in a change of other surface qualities in addition to roughness due to the surface roughening procedure. The machined Ti disks were roughened by sandblasting and electric glow discharging. The surface properties of the Ti specimens were inspected through a comprehensive surface analysis. MG63 cell behaviors were compared along with cell number, alkaline phosphatase (ALP) activity, Runx2 gene expression, and type I collagen production. Statistics were evaluated, using analysis of variance (ANOVA). The sandblasted Ti disks demonstrated well-controlled surface roughness features and meaningful average roughness ranges, including the surface roughness of the “modern” microrough implant, used clinically. With increasing Ti surface roughness, the cell number decreased, while the ALP activity, type I collagen production, and Runx2 gene expression increased significantly. The rougher the Ti surface was, the sooner the Runx2 gene was expressed. Based on these results, we suggest that the microrough Ti surfaces of the 1–3 μm range may contribute effectively to osteogenic differentiation and proliferation in MG63 cells. © 2006 Wiley Periodicals, Inc. J Biomed Mater Res, 2006</P>
Cho, Seung-Woo,Lim, Joung Eun,Chu, Hun Su,Hyun, Hye-Jin,Choi, Cha Yong,Hwang, Ki-Chul,Yoo, Kyung Jong,Kim, Dong-Ik,Kim, Byung-Soo Wiley Publishers 2006 Journal of Biomedical Materials Research Part A Vol. No.
<P>Successful reconstruction of large-diameter blood vessel in humans has been demonstrated using the tissue engineering technique, but improvement in patency of small-diameter bioartificial vascular graft remains a great challenge. This study reports that granulocyte colony-stimulating factor (G-CSF) can enhance in vivo endothelialization of tissue-engineered vascular grafts, which could be used to improve patency of small-diameter vascular graft. Vascular grafts were tissue engineered with decellularized canine abdominal aortas and canine autologous bone marrow–derived cells. Prior to cell seeding onto decellularized graft matrices, bone marrow–derived cells were induced to differentiate into endothelial cells and smooth muscle cells. The cell-seeded vascular grafts were implanted into the abdominal aortas of bone marrow donor dogs. Before and after graft implantation, G-CSF was administered subcutaneously to the dogs (n = 3). The grafts implanted into the dogs not receiving G-CSF were used as controls (n = 3). Eight weeks after implantation, grafts in both groups showed regeneration of vascular tissues including endothelium and smooth muscle. Importantly, endothelium formation was more extensive in the G-CSF–treated grafts than in the control grafts, as assessed with reverse transcription polymerase chain reaction, western blot, and immunohistochemistry. In addition, intimal hyperplasia was significantly reduced in the G-CSF–treated grafts compared to the control grafts. This study suggests that G-CSF administration could be applied to improve patency of small-diameter tissue-engineered vascular grafts. © 2005 Wiley Periodicals, Inc. J Biomed Mater Res, 2006</P>
Yang, Jaemoon,Cho, Eun-Jin,Seo, Sungbaek,Lee, Jae-Won,Yoon, Ho-Geun,Suh, Jin-Suck,Huh, Yong-Min,Haam, Seungjoo Wiley Publishers 2008 Journal of Biomedical Materials Research Part A Vol. No.
<P>Folate (FA) conjugated tri-block copolymers were prepared by bioconjugation of poly ϵ-caprolactonediol and various molecular weights of diamine polyethylene glycol. The synthetic tri-block copolymers were characterized by <SUP>1</SUP>H-NMR. Three types of nanoparticles were prepared by nanoprecipitation. Their size and morphology were verified by laser scattering and transmission electron microscopy, respectively. The colloidal stability of the nanoparticles was evaluated by turbidity test. The anticancer drug doxorubicin (DOX) was encapsulated in the nanoparticles during preparation. Drug loading amounts and release behavior from prepared nanoparticles were investigated. Fluorescent-activated cell sorting analysis and epi-fluorescencic microscopic imaging of prepared nanoparticles exhibited good cellular uptake against target cells. FA receptor expressed OVCAR3 cells that showed higher mean fluorescence intensity than FA receptor defect A549 cells at specific polyethylene glycol chain lengths. The cell cytotoxicity of prepared nanoparticles was evaluated for receptor mediated drug delivery. © 2007 Wiley Periodicals, Inc. J Biomed Mater Res, 2008</P>