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이주희(Jue Hee Lee),심진기(Jin Kie Shim),한용문(Young Moon Han) 대한약학회 2005 약학회지 Vol.49 No.5
Our previous data showed the protein isolated from Coptidis Rhizoma(CRP) had antifungal activity. In present study, we examined mode of action of the CRP and its activity against subcutaneous candidiasis 엳 새 C. albicans yeast cells. Results showed that the CRP blocked hyphal production from yeast form of C. albicans. The CRP also activated RAW 264.7 monocyte/macrophage cell line, which resulted in nitiric oxide (NO) production from the cells. This activation seemed to increase macrophage phagocytosis to destroy the invaders. Like other antimicrobial peptides, CRP was influenced by ionic strength, thus resulting in a decrease of antifungal activity. In murine model of a subcutaneous candidiasis, the sizes of infected areas of the nude mice given the CRP after subcutaneous injection of C. albicans yeast cells to the dorsal skin were 90% less than those of the nude mice groups that received DPBS instead of the CRP. All data indicate that the CRP, which appeared to act like an antimicrobial peptide and to inhibit the morphological transition from blastoconidia, was effective against the subcutaneous disease.
가역적 온도감응성을 가지는 가교된 나노운반체의 제조와 그 특성
이상봉 ( Sang Bong Lee ),심진기 ( Jin Kie Shim ),정석진 ( Suk Jin Chung ) 한국키틴키토산학회 2010 한국키틴키토산학회지 Vol.15 No.4
하한임계온도를 가지는 PNIPAAm과 Chitosan을 포함하는 공중합체로 이루어진 약물전달체는, 온도변화에 따라 가역적으로 입자의 형성 및 방출 거동을 보이므로, LCST 이하에서 약물전달체 입자를 형성 할 수 있을 뿐만 아니라 PNIPAAm의 분자길이 조절을 통해 약물 전달체 크기 또한 결정 할 수 있어 약물의 포집 및 방출이 용이한 효율적인 약물전달체로 이용될 수 있다. 개발된 약물전달체는 공중합 반응의 반응비를 제한함으로써 나노수준의 입자 크기를 가지므로, 생물의 원형질막을 통과할 수 있는 효과가 기대 된다. 또한 약물전달 체의 표면 가교를 통해 온도변화에 무관하게 입자상태를 유지할 수 있었으므로 약물 포집상태의 안정성을 효과적으로 높일 수 있었다. This study is about drug delivery system (DDS) using copolymer of hydrophilic and temperature sensitive polymer. The copolymer was maintained the particle form by crosslinking of the surface at temperature lower than lower critical solution temperature (LCST) and it has thermo-sensitivity on changing the size of the particle above LCST. The developed copolymer is obtained or loss the amphiphilic by changing temperature then it is possible to form of the reversible particle size. Thus it can be used as a nano-carrier for drug delivery system by collected drugs. In this study, the nano-carrier can do drug delivery even under LCST.
김현경(Hyunkyung Kim),이주희(Jue Hee Lee),심진기(Jin Kie Shim),한용문(Young Moon Han) 대한약학회 2005 약학회지 Vol.49 No.4
Antimicrobial peptides are evolutionary ancient weapons for animal and plant species to depend themselves against infectious microbes. In the present study, we investigated if an antimicrobial peptide was produced from Coptidis Rhizoma. For the determination, protein substance from the medicinal plant was isolated by various preparations. Among the preparations, the protein portion dissolved in phosphate-buffered saline solution (CRP-DS) that contained the most amount of protein (90%) resulted in maximal inhibition of Candida albicans which causes local and systemic infections. Analyses by gel-electrophoresis and gel-permeation chromatography showed the CRP-DS formed a single band of approximately 11.8 KDa as molecular size. Antifungal activity of the CRP-DS was almost equivalent to antifungal activity by fluconazole, resulting in MIC (minimal inhibitory concentration) of approximately 50 ㎍/㎖. The antifungal activity was a dose-dependent. The antifungal activity appeared to be inactivated by heat-treatment and ionic strength, respectively. In a murine model, the CRP-DS enhanced resistance of mice against disseminated candidiasis. The HPLC analysis demonstrated maximum 4% of berberine as residual content in the CRP-DS preparation resulted in no influence on the antifungal activity. In addition, protein portion isolated from Phellodendri Cortex producing the alkaloid component like Coptidis Rhizoma had no such anticandidal effect. These results indicate that the protein substance from Coptidis Rhizoma was responsible for the antifungal activity.
이동열(Dong Yeol Lee),차기철(Gi Cheol Cha),심진기(Jin Kie Shim),이영무(Yong Moo Lee),유익근(Ik Keun Yoo),안승호(Seung Ho Ann) 한국물환경학회 2000 한국물환경학회지 Vol.16 No.2
Membrane Bioreactor (MBR) process for food wastewater was studied to find the treatment chracteristics and the operating parameters at the hydraulic retention time (HRT) of 0.5 and 0.25day. Plate and frame membrane module was submerged in laboratory-scale MBR of 12L volume. Membrane suction-idle operation of l0minutes-on and 5minutes-off cycle showed the stable permeate flux and suction pressure. The BOD and COD removal efficiencies were obtained over 96% and 91%, respectively. During the operation, the increase of E260/DOC ratio (scale of non-degradable organic matter) was observed in the MBR. The resistance value of cake layer was measured 1.24 X 10^(11) (1/m) and this value showed more significant affect on flux drop than that of among other resistance layers.