청열해독용 한약재의 항진균작용에 의한 항감염성 관절염 효과검색

공수현,이주영,박지혜,김송이,이주희,한용문 동덕여자대학교 종합약학연구소 2007 동덕약학연구지 Vol.11 No.-

In present study, we tested antifungal effects of 11 different kinds of compounds isolated from Phellodendri Cortex (PC), Lonicerae Flos (LF), Lonicerae Folium et Caulis (LFC) and Araliae Continental is Radix (ACR). These herbal medicines are generally applied for the treatment of inflammations. Prior to the test, antifungal effect of 70%-ethanol extracts of the medicines was examined. Results showed that the three extracts of PC, LF and LFC, but not ACR extract, inhibited Candida albicans growth, in-vitro. Among the 11 compounds, berberine and palmatine isolated from PC showed the most antifungal activity (P < 0.05). Lonicerin and loganin from LF and chlorogenic acid from LFC also retarded growth of C. albicans at high dose of 250 ㎛/ml, but their activity was much less than berberine or palmatine. However, ent-Pimara-8(14),15-diene-19-oic acid and 16α,17- Dihydroxy-ent-kauran-19-oic acid from ACR had no antifungal activity as expected. All together, these data imply that most of oriental herbal medicines having anti-inflammatory activity could have anticandidal effect, suggesting that PC, LF and LFC be effective for the treatment of septic arthritis by killing of C. albicans.

흑미의 도정분획 메탄올 추출물의 항산화 성분 및 항산화 효과

공수현(Suhyun Kong),최용민(Youngmin Choi),이선미(Seon-Mi Lee),이준수(Junsoo Lee) 한국식품영양과학회 2008 한국식품영양과학회지 Vol.37 No.7

본 연구에서는 흑미 두 품종(흑진주벼와 흑광벼)의 도정 분획별 메탄올 추출물의 항산화 성분과 항산화 활성을 비교ㆍ분석하고자 하였다. 흑미의 항산화 성분으로 polyphenolic, flavonoid, anthocyanin과 γ-oryzanol, tocopherol 및 tocotrienol 함량을 측정하였으며 항산화 활성은 DPPH와 ABTS radical 제거능을 측정하였다. 각 도정 분획별 메탄올 추출물에서 rice bran 층에서 약 15.3~15.5%의 가장 높은 수율을 보였으며 endosperm 메탄올 추출물에서 약 0.9~1.1%의 가장 낮은 수율을 보였다. 실험결과 rice bran의 추출물에서 endosperm, whole grain의 추출물보다 높은 함량의 항산화 성분이 분포함을 확인할 수 있었다. 또한 이러한 항산화 성분이 다량 분포하는 rice bran 추출물의 우수한 항산화력을 측정할 수 있었다. 품종 간에는 흑진주벼의 rice bran 층의 항산화 성분이 흑광벼보다 높은 함량을 나타내었다. 본 연구결과 흑미의 rice bran은 기능성식품의 성분으로 활용가능성이 높을 것으로 예상된다. The colored rice, particularly black rice, has been well characterized to possess antioxidant properties. Rice bran, a by-product of the rice milling process, contains high levels of several phytochemicals which have antioxidant activities as well as health-beneficial properties. The purposes of this study were to evaluate the antioxidant compounds and antioxidant activities of the methanolic extracts from milling fractions and whole grain of two black rice cultivars. Whole black rice of the two cultivars were milled into rice bran and endosperm using the gradual milling system. These were evaluated for antioxidative activities by DPPH and ABTS radical scavenging activities. To determine the antioxidant compounds in the methanolic extract from the milling fractions, the content of polyphenolics, flavonoids, anthocycanins and γ-oryzanol were measured by spectrophotometric methods and vitamin E analysis was carried out by HPLC. The yield of whole black rice, rice bran, and endosperm were 3.1, 15.3, and 0.9% for Heugjinjubyeo and 2.7, 15.5, and 1.1% for Heugkwangbyeo, respectively. The methanolic extracts from rice bran showed generally higher antioxidant activities than the extracts from whole grain and endosperm. In addition, antioxidant compounds distributed much higher contents in rice bran extract than in the extracts from whole grain and endosperm. A significant correlation was also noted between free radical scavenging activity and polyphenolic compounds. The results of this study show that notable antioxidant activity in black rice bran are considered to have significant health benefits.

발아현미 첨가에 따른 국수의 제조 특성과 GABA 함량 및 항산화 활성 변화

공수현(Suhyun Kong),이준수(Junsoo Lee) 한국식품영양과학회 2010 한국식품영양과학회지 Vol.39 No.2

발아 전과 후의 현미와 미강을 첨가한 밀가루 복합분으로 만들어진 국수의 품질 특성과 GABA 함량 및 항산화 활성의 변화를 조사하였다. 발아 전후의 현미와 미강을 각각 5, 10, 15% 첨가하여 국수를 제조하였다. 이렇게 제조된 국수는 조리 및 제면특성(cooking loss, swelling index, water holding capacity), 조리에 따른 GABA 함량과 항산화 성분(polyphenol) 및 활성(ABTS와 DPPH의 radical scavenging activities) 변화를 통해 기능성 국수로의 이용가능성을 알아보고자 하였다. 실험결과, 발아 후 rice bran의 첨가에 따른 cooking loss가 증가하는 것으로 관찰되었으며 반면에 swelling index에서는 차이를 나타내지 않았다. 발아 후 현미와 미강을 첨가한 조리 전후 국수에서 발아 전 현미와 미강을 첨가한 국수보다 높은 GABA 함량을 확인하였으며 발아에 따른 polyphenol 함량과 항산화 활성에서는 큰 변화가 없음을 확인할 수 있었다. 따라서 발아현미가 첨가된 국수는 밀가루 국수의 영양학적 가치와 항산화 기능을 향상시킬 수 있는 소재로써 이용 가능할 것으로 생각된다. This study was to investigate the quality characteristics of noodles prepared with germinated brown rice and rice bran, and to determine the changes in GABA content and antioxidant activity of the noodles. These noodles were evaluated for cooking properties (cooking loss, swelling index, water holding capacity), total polyphenols, antioxidant activities (radical scavenging of ABTS and DPPH), and GABA contents. The addition of germinated rice bran in the range of 5, 10, and 15% showed a significant influence on cooking loss, although no effect on swelling index was observed. The raw and cooked noodles containing germinated brown rice and rice bran contained more GABA contents (2751.6~4176.7 and 5522.0~9617.8 nmol/20 g of fresh noodle, respectively) than those of non-germinated brown rice and rice bran. Noodles made with germinated brown rice and rice bran had similar total polyphenolics contents and antioxidant activities compared to noodles made with non-germinated brown rice and rice bran. Overall, the germinated brown rice could be used as a good ingredient to increase the nutritional value and antioxidant properties of wheat flour noodle without affecting the cooking properties.

금종쌀 Methanol 추출물의 항산화 성분 및 항산화 효과

공수현(Suhyun Kong),최용민(Youngmin Choi),김영화(Youngwha Kim),김대중(Dae-Jung Kim),이준수(Junsoo Lee) 한국식품영양과학회 2009 한국식품영양과학회지 Vol.38 No.6

본 연구에서는 도정되지 않은 현미상태의 금종쌀(Geumjong brown rice) methanol 추출물의 항산화 성분과 항산화활성을 측정하여 새로운 품종의 기능적 가치를 평가하고자 하였다. 이와 함께 백미(Chuchung white rice) 및 현미(Chuchung brown rice)와의 항산화 성분과 항산화력을 비교ㆍ분석하고자 하였다. 항산화 성분으로는 polyphenol, flavonoid, vitamin E와 γ-oryzanol을 측정하였으며 항산화력은 ABTS와 DPPH 라디칼 제거능 및 환원력을 측정하였다. 백미 및 현미, 금종쌀은 각각 1.5, 2.3, 그리고 3.5%의 추출수율을 나타냈다. 실험결과, 금종쌀은 높은 항산화 활성(69.77 ㎎ TEAC/100 g)과 polyphenol함량(69.1 ㎎ gallic acid equivalents/100 g)을 나타냈다. 반면에 환원력에 있어서는 현미(Chuchung brown rice)보다 낮게 측정되었다. In this study, the antioxidant compounds and antioxidant activities of Geumjong brown rice were measured to evaluate functional properties and to compare with white rice and Chuchung brown rice. The content of polyphenolics, flavonoids and γ-oryzanol were measured by spectrophometric methods and tocopherol and tocotrienol analyses were carried out by HPLC. ABTS and DPPH radical scavenging activity and reducing power were used to measure antioxidant activity. The extraction yield of Chuchung white rice, Chuchung brown rice, and Geumjong brown rice were 1.5, 2.3, and 3.5%, respectively. The high level of antioxidant activity (69.77 ㎎ TEAC/100 g) and total polyphenolic content (69.1 ㎎ gallic acid equivalents/100 g) were found in Geumjong brown rice, whereas the reducing power of Geumjong brown rice was lower than that of Chuchung brown rice.

발아에 따른 일부 특수미의 GABA 함량 변화

최용민,전건욱,공수현,이준수 한국산업식품공학회 2009 산업 식품공학 Vol.13 No.2

일반적으로 현미를 발아시킬 경우 다양한 효소의 활성화로 인하여 GABA, ferulic acid, arabinoxylan, inositol 등의 기능성 성분들이 증가될 뿐만 아니라 조직을 연화시켜 현미의 식미를 개선시키는 것으로 알려져 있다. GABA는 발아현미에 존재하는 기능성 물질 중의 하나로 비단백질 아미노산이다. 본 연구의 목적은 발아 후 품종별 GABA 함량의 변화를 측정하고자 하였으며 이와 함께 vitamin E의 변화와 항산화력의 변화를 측정하고자 하였다. GABA 함량은 비색법을 이용하였고 vitamin E는 HPLC 방법을 항산화력은 ABTS와 DPPH 라디칼 소거능을 이용하여 측정하였다. 본 연구 결과 품종별 현미는 발아 과정을 통하여 GABA 함량은 약 10배 이상 그리고 vitamin E의 경우 tocotrienol이 상당히 증가하였으며 항산화 활성에는 전반적으로 변화가 없는 것으로 나타났다. 특히 큰눈 발아 현미의 경우 GABA 함량이 다른 품종에 비해 월등히 높았고 vitamin E 함량과 항산화 활성은 신명흑찰 발아현미가 높은 값을 나타내었다. 본 연구결과 현미를 발아시키면 GABA 및 vitamin E 등과 같은 생리활성 물질이 증가되고 항산화 활성이 그대로 유지된 기능성 쌀 가공품의 원료를 얻을 수 있을 것으로 생각한다. The purposes of this work were to investigate the changes in GABA content of six different rice cultivars along with viatmin E content and antioxidant activity after germination. Brown rice was soaked for 24 hr at 25oC and then germinated at 37oC for 48 hr. The content of GABA and vitamin E in the rice samples was measured by using spectrophotometeric and HPLC methods, respectively. Antioxidant activity was measured by ABTS (2,2’-azino-bis-(3-ethylbenzothiazoline-6-sulphonic acid)) and DPPH (1,1-diphenyl-2-picrylhydrazyl) radical scavenging methods. GABA and vitamin E contents were significantly increased after germination while no significant change in the antioxidant activity was observed. Among the samples tested, Geunnun cultivar contained the highest GABA content before and after germination. On the other hand, Sinmyungheugchal cultivar showed the highest content of vitamin E and antioxidant activity compared to other rice cultivars. In conclusion, the germinated rice with high GABA content can be used for a functional ingredient in rice processing industry.

Chlorogenic acid Rutin의 Macrophage 및 T-임파구 증식에 대한 효과

박지혜,공수현,이주영,김송이,이주희,한용문 동덕여자대학교 종합약학연구소 2007 동덕약학연구지 Vol.11 No.-

In the present study, we examined immunoregulatory activitres of cholrogenic acid (CA) and rutin that are isolated from Lonicera Flos (A family of Caprifoliaceae). For the examination, NO production of LPS-treated RAW264.7 monocyte/macrophage cell line and effect on the T-cells proliferation were determined. Results showed that CA inhibited NO production at low concentrations below 10 ㎛/ml, but at higher concentration ranged from 10 ㎛/ml to 40 ㎛/ml the inhibitory activity was lost. However, rutin blocked the NO production in a dose-dependancy. In case of T-cell proliferation, both of the compounds suppressed the proliferation (P < 0.05). Combined together, it is assumed that the compounds might be effective to arthritic inflammation, possibly, by removal of CD4+ T-cells, but rutin might be more effective against the inflammation because of the blockage of NO production from macro phages which is responsible for the pain during the process of arthritis.

Photonic diode behavior in energy-graded GaN/InGaN core-shell quantum well rod grown by metal-organic chemical vapor deposition

고석민,공수현,조용훈 한국진공학회 2014 한국진공학회 학술발표회초록집 Vol.2014 No.8

비공진 연속파 광펌핑을 이용한 엑시톤-폴라리톤 양자유체 형성

권민식,오병용,공수현,김제형,강항규,강수석,송진동,최형순,조용훈 한국진공학회 2017 한국진공학회 학술발표회초록집 Vol.2017 No.8

화학적 기상 에칭법을 이용한 고품질 질화물 반도체 나노구조 형성 연구

김제형,고영호,공수현,고석민,오충석,박기연,정명호,이정용,조용훈 한국진공학회 2012 한국진공학회 학술발표회초록집 Vol.2012 No.8

Efficient Red-Color Emission of InGaN/GaN Double Hetero-Structure Formed on Nano-Pyramid Structure

고영호,김제형,공수현,김주성,김택,조용훈 한국진공학회 2012 한국진공학회 학술발표회초록집 Vol.2012 No.8