폐렴구균 DNA 백신의 면역병리학적 측면에서의 안전성 평가

이주희(Jue Hee Lee),한용문(Young Moon Han) 대한약학회 2006 약학회지 Vol.50 No.1

We have previously reported the minimum criteria that can be applied to evaluate efficacy and safety of a DNA vaccine with use of Streptococcus pneumoniae DNA vaccne (SPDNA). The SPDNA was formulated by inserting the DAN sequences that are codons specific for the carbohydrate epitope in the capsule of S. penumoniae by phage display peptide library. Administration of the SPDNA into mice induced both humoral and cell-mediated immunities. The induction was protective even in the absence of CD4+ T lymphocyte in mice. Profiles of cytokine and isotyping of antibody displayed tendency of the Th1. In continuation of these studies, we examined if the efficacy of the SPNDA was provoked by the peptide recognized by codons specific for the capsule. Results showed that the peptide vaccine formulae (SPP) induced protective antibody in mice as did the SPDNA. Involvement of the cell-mediated immunity was also determined. Possible side effects of autoimmune diseases such as myositis and C3a production and tumor-formation were undetectable in mice given 7 times of SPDAN vaccination during entire of 92 days. Even after the frequent immunization, immunogenicity of the SPDNA was observed as determined for antibody production, suggesting that there was no immunotolerance provoked. All together, these examining factors would be applied to measurement of DNA vaccine safety regarding the immunopathological aspect.

도시 대기중 수은 농도 경인지역을 중심으로

손동헌(Dong Hun Sohn),조관영(Kuan Young Cho),한용문(Yong Moon Han) 대한약학회 1986 약학회지 Vol.30 No.6

Atmospheric mercury was collected by gold amalgamation method and analyzed by cold flameless atomic absorption spectrometry. The distribution of atmospheric mercury was investigated at seven location (rural I, II, industrial I, II, University and the center of urban). The amount of atmospheric mercury collected at the rural area I (Mt. Seolack) ranged from 0.7 to 1.8ng/m3, and mean value was 1.2ng/m3 (n=4), at the rural area II (Buyeo) ranged from 0.8 to 5.1ng/m3, and mean value was 2.Ong/m3 (n=8), at the area of the center of urban (Jong-ro 3 Ga, Seoul) ranged from 22.7 to 43.1ng/m3, and mean value was 35.1ng/m3 (n=12), at the residential area I (Galak Dong, Seoul) ranged from 2.2 to 5.1ng/m3, and mean value was 3.5ng/m3 (n=6) at the Chung-Ang University (Heukseok Dong, Seoul) ranged from 2.2 to 176.2ng/m3, mean value was 36.8ng/m3 and median value was 16.0ng/m3 (n=188), at the residential area II (Goowal Dong, Inchon) ranged from 6.4 to 20.7ng/m3, and mean value was 13.1ng/m3 (n=10), and at the industrial area (Songhyun Dong, Inchon) ranged from 13.9 to 88.3ng/m3, and mean value was 38.9ng/m3 (n=12). The behavior of atmospheric mercury concentration at Chung-Ang University showed high in day time and summer, but low in night-time and winter.

황련에서 분리된 단백질성분의 항진균효과

김현경(Hyunkyung Kim),이주희(Jue Hee Lee),심진기(Jin Kie Shim),한용문(Young Moon Han) 대한약학회 2005 약학회지 Vol.49 No.4

Antimicrobial peptides are evolutionary ancient weapons for animal and plant species to depend themselves against infectious microbes. In the present study, we investigated if an antimicrobial peptide was produced from Coptidis Rhizoma. For the determination, protein substance from the medicinal plant was isolated by various preparations. Among the preparations, the protein portion dissolved in phosphate-buffered saline solution (CRP-DS) that contained the most amount of protein (90%) resulted in maximal inhibition of Candida albicans which causes local and systemic infections. Analyses by gel-electrophoresis and gel-permeation chromatography showed the CRP-DS formed a single band of approximately 11.8 KDa as molecular size. Antifungal activity of the CRP-DS was almost equivalent to antifungal activity by fluconazole, resulting in MIC (minimal inhibitory concentration) of approximately 50 ㎍/㎖. The antifungal activity was a dose-dependent. The antifungal activity appeared to be inactivated by heat-treatment and ionic strength, respectively. In a murine model, the CRP-DS enhanced resistance of mice against disseminated candidiasis. The HPLC analysis demonstrated maximum 4% of berberine as residual content in the CRP-DS preparation resulted in no influence on the antifungal activity. In addition, protein portion isolated from Phellodendri Cortex producing the alkaloid component like Coptidis Rhizoma had no such anticandidal effect. These results indicate that the protein substance from Coptidis Rhizoma was responsible for the antifungal activity.

황련단백질의 황캔디다 작용기전 및 항피부캔디다증 효과

이주희(Jue Hee Lee),심진기(Jin Kie Shim),한용문(Young Moon Han) 대한약학회 2005 약학회지 Vol.49 No.5

Our previous data showed the protein isolated from Coptidis Rhizoma(CRP) had antifungal activity. In present study, we examined mode of action of the CRP and its activity against subcutaneous candidiasis 엳 새 C. albicans yeast cells. Results showed that the CRP blocked hyphal production from yeast form of C. albicans. The CRP also activated RAW 264.7 monocyte/macrophage cell line, which resulted in nitiric oxide (NO) production from the cells. This activation seemed to increase macrophage phagocytosis to destroy the invaders. Like other antimicrobial peptides, CRP was influenced by ionic strength, thus resulting in a decrease of antifungal activity. In murine model of a subcutaneous candidiasis, the sizes of infected areas of the nude mice given the CRP after subcutaneous injection of C. albicans yeast cells to the dorsal skin were 90% less than those of the nude mice groups that received DPBS instead of the CRP. All data indicate that the CRP, which appeared to act like an antimicrobial peptide and to inhibit the morphological transition from blastoconidia, was effective against the subcutaneous disease.

대퇴골두 골괴사를 가진 환자에서의 조립식 대퇴주대를 이용한 무시멘트형 고관절 전치환술 -금속 금속 관절면과 세라믹 세라믹 관절면의 비교-

강번중 ( Bun Jung Kang ),이주희 ( Jue Hee Lee ),정영복 ( Young Bok Jung ),김진홍 ( Jin Hong Kim ),한용문 ( Yong Moon Han ),장의찬 ( Eui Chan Jang ),김경운 ( Kyoung Woon Kim ) 대한고관절학회 2011 Hip and Pelvis Vol.23 No.4

목적: 진행된 대퇴골두 골괴사증 환자에서 무시멘트형 조립식 대퇴주대를 이용한 고관절 치환술의 임상적 및 방사선학적 결과를 평가하였으며, 금속-금속 관절면을 사용한 군과 세라믹-세라믹 관절면을 사용한 군 간, 결과에 차이가 있는지를 확인하고자 하였다. 대상 및 방법: 2001년 1월부터 2004년 12월 까지 대퇴골두 골괴사증으로 조립식 대퇴주대를 이용하여 무시멘트형 고관절 치환술을 시행 받았으며, 5년 이상 추시관찰이 가능하였던 66명(78예)을 대상으로 하였고, 술 후 평균 추시 기간은 평균 77개월(60~122개월) 이었다. 결과: 임상적 평가로 술 전 평균 Harris 고관절 점수는 53점에서 최종 추시 시 88.5점으로 향상되었다. 방사선학적 검사 결과에서 대퇴주대의 안정도는 68예에서 골성고정을 얻었으며, 9예에서 섬유성 안정고정, 1예에서 불안정고정을 나타내었다. 재치환술은 3예에서 시행하였으며, 122개월 간 대퇴 스템의 생존율은 95.7%인 것으로 조사되었다. 결론: 진행된 대퇴골두 골괴사증에서 시행한 조립식 대퇴주대를 이용한 무시멘트형 고관절 전치환술은 5년 이상 추시 결과 금속-금속 관절면을 사용한 군과 세라믹-세라믹 관절면을 사용한군, 모두 만족한 만한 임상적 및 방사선학적 결과를 얻었다. Purpose: The purpose of this study was to evaluate the clinical and radiological outcomes after total hip arthroplasty using the S-ROM modular system for osteonecrosis of the femoral head, and to compare the results between the groups using metal-on-metal articulation and ceramic-on-ceramic articulation. Materials and Methods: Sixty-six patients (78 hips) with osteonecrosis of the femoral head were evaluated after primary total hip arthroplasty between January 2001 and December 2004, using an S-ROM proximal modular femoral stem. The average follow-up was 77 months (range, 60 to 122 months) and all patients were followed for more than five years. Results: The average Harris hip score improved from 53 points to 88.5 points at the final follow-up. At the latest radiologic evaluation, sixty-seven stems had bony ingrowth stability, and 10 stems had stable fibrous ingrowth. However, one stem had diffuse extensive osteolysis and loosening, which was revised at 9 years. Postoperative complications included 4 cases of heterotrophic ossificiation, 1 case of linear fracture after insertion of the femoral stem, 1 case of dislocation, 2 cases of infection, and 1 case of extensive osteolysis and loosening. There were 3 cases of revision and Kaplan-Meier survivorship analysis with revision estimated at a 95.7% chance of survival for the femoral component during 122 months. Conclusion: Our study showed that total hip arthroplasty using the S-ROM modular system with metal-on-metal articulation or ceramic-on-ceramic articulation had favorable clinical and radiological mid- to long-term results.