Plant regeneration from callus of Iris odaesanensis Y. N. Lee native to Korea via organogenesis

Bae, Kee-Hwa,Yoo, Kyoung-Hwa,Lee, Mi-Hyun,Jeong, Jae-Hun,Choi, Yong-Eui,Yoon, Eui-Soo The Korean Society of Plant Biotechnology 2013 식물생명공학회지 Vol.40 No.3

Iris odaesanensis Y. N. Lee. is an important endangered and native plant belonging to the family Iridaceae in Korea. This study describes a method for rapid micropropagation of this species via from leaf, rhizome and root explants derived calli. Leaf, rhizome and root explants were cultured on Murashige and Skoog (MS) medium supplemented with 2,4-dichlorophenoxy acetic acid (2,4-D) for callus induction. Rhizome explants yielded calli at a frequency of 72% when cultured at 1.0 mg/l 2,4-D. Calli were maintained at 1.0 mg/l 2,4-D. These calli were transferred to MS medium supplemented with 0, 0.5, 1.0, and 2.0 mg/l 2,4-D in combination with 0, 0.5, 1.0, and 3.0 mg/l BA for adventitious shoot induction. The highest number of adventitious shoot (228.9 per petri-dish) were formed at 1.0 mg/l 2,4-D and 1.0 mg/l BA. WPM medium was the best to convert calli into plantlets, where up to 98.2% of calli were regenerated into plantlets. This in vitro propagation protocol should be useful for conservation of this endangered plant.

간호 학생의 임상실습 효율을 위한 기초 조사 연구

이애경,김정애,주미경,정안순,배경진,김정수,이정애,장은정 경복대학 2000 京福論叢 Vol.4 No.-

임상실습 교육은 간호이론을 실무에 적용하는 기회를 제공하는 과정으로서 임상실습이 간호교육의 중요한비중을 차지하고 있다는 것은 주지의 사실이다. 이러한 중요성에도 불구하고, 임상실습 교육에 즉각적인 해결이 어려운 많은 문제점이 있음도 이미 알고 있는 사실이다 이에 임상실습 교육의 기초 자료로 활용되고 있는 실습목록표에 대한 간호 학생들의 실습목표의 명확한 이해와 더불어 이론과 실습의 연계성을 강화시키고자 하였다. 성인간호, 모성간호, 이동간호 3개 영역 모두에서 공통적으로 기본임상간호 항목이나 기본간호술과 관련된 항목에서 직접수행의 빈도가 높았고 숙련된 기술적인 처치나 전문성을 필요로 하는 항목에서는 관찰과 수행경험이 부족한 것으로 나타났다. Clinical performance is not only the process of providing the opportunity for applying nursing theory into practice but also it is a well-known fact that clinical performance is an important part in nursing education. lnspite of this importance, it is also true that many problem with no immediate solution exist in clinical performance of nursing students. thus, through measuring and analyzing the degree of clinical experience of the nursing students, on the nursing checklist that is being used as the basic guide in clinical education, we tried to clearly understand the objectives of clinical performance and to emphasize the connection between theory and clinical performance. The results of the study showed that the frequency of directly performing tasks was high in all areas of adult health nursing, maternity nursing, and child nursing for the items of basics clinical nursing and items related with basic nursing techniques, and that the experiences of observation and execution were lacking in the items needing skilled treatment or speciality.

Dimethylnitrosamine 유발 급성 간 손상 흰쥐에서 ^(99m)Tc-Lactosylated Serum Albumin을 이용한 간 기능의 평가

정신영,이재태,서명랑,유정아,배진호,안병철,황재석,정재민,하정희,이규보 대한핵의학회 2003 핵의학 분자영상 Vol.37 No.6

목적: ^(99m)Tc-lactosylated serum albumin (^(99m)Tc-LSA)은 간세포에 특이적으로 결합하는 간수용체 영상용 방사성의약품으로 새로이 합성되었다. 간섬유화를 유발하는 dimethylnitrosamine (DMN)을 투여한 간 손상 휜쥐 모델에서 ^(99m)Tc-LSa의 역동학적인 간섭취를 조사하고 간효소치의 변화와 조직학적 소견을 비교하여, LSA의 간섭취가 간기능의 변화를 반영하는지를 연구하였다. 대상 및 방법: SD계 흰쥐에 DMN를 27 mg/kg으로 복강 내 주사하여 급성 간손상을 유도하고 대조군과 비교하였다. DMN을 주사한 흰쥐를 3일(DMN-3), 8일(DMN-8), 21일(DMN-21)에 ^(99m)Tc=LSA (1,665 mg/kg) 29 MBq를 정맥 주사하여, 30분 동안 동적 영상을 획득하고 간과 신장부위에 관심영역을 설정하여 간과 심장부위의 시간방사능 곡선을 얻었다. 간기능 평가를 위해 시간방사능 곡선을 이용하여 간섭취지수와 혈중제거지수를 구하였고 곡선 최적화를 시행하였다. DMN 투여군과 대조군의 간효소치의 변화와 간조직의 광학현미경 소견을 비교하였다. 결과: 대조군에서는 ^(99m)Tc-LSA가 빠르게 간에 섭취되고 혈중에서 제거되었으나 DMN을 처리한 군에서는 간섭취가 낮았다. 간섭취지수의 비교에서 대조군에 비해 DMN 처리군에서 유의하게 간섭취지수가 낮았다(DMN-3: 0.842, DMN-8: 0.898, DMN-21: 0.91, 대조군: 0.96, p<0.05). 혈중제거지수의 비교에서도 대조군에 비해 DMN 처리군에서 혈중제거지수가 유의하게 높았다(DMN-3: 0.731, DMN-8: 0.654, DMN-21: 0.604, 대조군: 0.473, p<0.05). 비선형 회귀분석에서 R_(2) 값은 0.9이상으로 좋은 일치를 보였고, 대조군에ㅓ K값이 DMN처리군에 비해 크고(DMN-3: 0.28, DMN-8: 0.41, DMN-21: 0.46, 대조군: 0.97, p<0.05), T_(1/2)값은 작았다(DMN-3: 2.5, DMN-8: 1.7, DMN-21: 1.5, 대조군: 0.7, p<0.05). 간효소치의 변화는 DMN-3군에서는 대조군에 비해 상승하였으나 DMN-8, DMN-21군에서는 간효소치의 상승이 관찰되지 않았다. 간조직 소견의 경우 DMN-3군에서 중심정맥 주위에 괴사가 관찰되었으나 DMN-8군, DMN-21군에서는 미약한 정도의 염증세포 침윤만이 관찰되었다. 결론: ^(99m)Tc-LSA 간신티그래피의 간섭취 정도는 간손상과 반비례하였으며 간섭취의 변화는 조직학적 손상이 심한 정도와 간손상후 회복되는 과정을 반영하여 주었다. ^(99m)Tc-LSA 간신티그래피가 간손상을 평가하고 간손상후 회복되는 과정을 추적하는 간수용체 영상용 방사성 의약품으로 사용될 수 있을 것으로 생각된다. Objects: ^(99m)Tc-lactosylated human serum albumin(LSA) is a newly synthesized radiopharmaceutical that binds to asialoglycoprotein receptors, which are specifically presented on the hepatocyte membrane. Hepatic uptake and blood clearance of LSA were evaluated in rat with acute hepatic injury induced by dimethylnitrosamine(DMN) and results were compared with corresponding findings of liver enzyme profile and these of histologic changes. Materials and Methods: DMN (27 mg/kg) was injected intraperitoneally in Sprague-Dawley rat to induce acute hepatic injury. At 3(DMN-3), 8(DMN-8), and 21(DMN-21) days after injection of DMN, LSA injected intravenously, and dynamic images of the liver and heart were recorded for 30 minutes. Time-activity curves of the heart and liver were generated from regions of interest drawn over liver and heart area. Degree of hepatic uptake and blood clearance of LSA were evaluated with visual interpretation and semiquantitative analysis using parameters (receptor index : LHL3 and index of blood clearance : HH3), analysis of time-activity curve was also performed with curve fitting using Prism program. Results: Visual assessment of LSA images revealed decreased hepatic uptake in DMN treated rat, compared to control group. In semiquantitative analysis, LHL3 was significantly lower in DMN treated rat group than control rat group (DMN-3:0.842, DMN-8: 0.898, DMN-21: 0.91, Control: 0.96, p<0.05), whereas HH3 was significantly higher than control rat group (DMN-3: 0.731, DMN-8: 0.654, DMN-21: 0.604, Control: 0.473, p<0.05). AST and ALT were significantly higher in DMN-3 group than those of control group. Centrilobular necrosis and infiltration of inflammatory cells were most prominent in DMN-3 group, and were decreased over time. Conclusion: The degree of hepatic uptake of LSA was inversely correlated with liver transaminase and degree of histologic liver injury in rat with acute hepatic injury.

보존적 치료로 회복된 망경색에 의한 급성복증 1예

김정념,이숭환,김정식,김진호,배윤오,박성규,윤상정,한현영,이헌영 충남대학교 의과대학 의학연구소 2003 충남의대잡지 Vol.30 No.1

Omental infarction, the end result of impaired perfusion to the greater omentum, is a rare benign self-limiting clinical entity. The main clinical symptom is non-specific localized abdominal pain with a moderately raised white blood cell and erythrocyte sedimentation rate. These findings often mimic an abdominal surgical emergency. This condition is often misdiagnosed as acute appendicitis or cholecystitis. The characteristic feature of CT scan and ultrasonography provide non-invasive diagnosis in most patients with omental infarction. We report a case of patient whose CT scan showed the characteristic finding of omental infarction. The patient was improved spontaneously only with conservative care.

Bortezomib과 Dexamthasone으로 치료한 골수외 형질세포종 4예

백종현,이은영,장리라,손창배,신은경,서정아,이지숙,이호섭,이상민,신성훈,김양수 고신대학교의과대학 2007 고신대학교 의과대학 학술지 Vol.22 No.2

Despite the use of aggressive local and systemic treatment including autologous stem cell transplantation in multiple myeloma, extramedullary recurrences are common and the prognosis of these patients is poor. Many novel drugs such as thalidomide, lenalidomide and bortezomib improve the response of treatment of multiple myeloma, but some reports failed to describe thalidomide has effect in extramedullary plasmacytoma. Recent data report on the successful treatment plasmacytomas with bortezomib in patients with advanced multiple myeloma. We treated 4 relapsed or refractory extramedullary plasmacytomas with bortezomib at our institution. We recognized all these extramedullary plasmacytomas decreased and showed more than partial response. This report lends support to the efficacy of bortezomib in the treatment of plasmacytoma and describes the safe use of bortezomib. Responses may, however, be of short duration. Therefore, despite our limited experience, we propose that bortezomib may be considered a therapeutic option for such patients who have risk of radiation therapy

한국 여성사 정립을 위한 여성인물 유형연구 Ⅳ : 1945년~1948년 The Period of United States Military Government

이배용,김정선,이승희,김점숙,김수자 이화여자대학교 한국여성연구원 1996 여성학논집 Vol.13 No.-

The target period of this study was the "Period of United States Military Government". This was the brief period from the declaration of military government by the U.S. Force stationed in the southern part of the Korean peninsula, with tile unconditional surrender of the Imperial Japanese Army until the establishment of the Republic of Korea as an independent entity on August 15, 1948. Despite its brevity, this period has been recognized as important, during which basic frameworks of capitalist economy and national authority were founded. This study explores women's lives and activities during the period and examines how the period restricted and reflected women's lives and activities. The content of the study is summarized as follows: The major trends that are significant during the period for women are: the expansion of education and acquiring of franchise by women. These were not a result of the women's movement, but of the policy of the US military government that claimed to establish and preserve "democratic order". The crucial problem at the time was, how women should utilize the given opportunities. Under the US military government, which advocated a liberal-democratic system, many changes were generated in consciousness along with changes in life style and other patterns. In particular, "equal lights between men and women" was thought of as a public value. Therefore the realization of "equal rights" was considered as a prerequisite for democratic order. In other words, the period was recognized as one of women's liberation from oppressive bonds, coinciding with liberation from Japanese Imperial Rule. In this period, western liberal culture was rapidly influencing South Korea and women's consciousness was thus also changed. Under these influences, a new image was emerging and making an impact on family structure. This was the image of progressive couples, wherein attempts were made to promote a new relationship of understanding and love between mothers-in-law and daughters-in-law who had traditionally been in conflict in the pre-modern family. During this period women became very active participants in socia1 spheres. In politics, women's activities in creating a nation state wet¡¤e significant. This was evident in the work of organizations in the women's movement. Their activities, however, were restricted by conflict between right and left wing political groups who were in acute confrontation with each other over the issue of deciding on a future direction for a new Korean nation state. This inevitably led to women's organizations focusing their energies on the establishment of the nation state, the hot issue of the period, and consequently, they abandoned the specific goals of the women's movement. In the meantime women who had obtained the right to vote could become the subjects as well as the objects of national politics, for instance, women took an active part in policy-making and there were 18 women candidates in the May 10th election under the military government. However, there were several harriers in women's be coming active agents in politics, for example, traditional concepts of "politics is a man's Job", a distrust of women's abilities and so on. The May 10th election was a memorable moment, when women used their votes for the first time in Korea and realized the aforementioned problems once again. While women failed to advance to the forefront of politics, yet this was a good opportunity for them to grapple with ways and means for reducing the barriers hindering women's political participation, while they Critically reviewed their past activities. In economic sectors, women were active participants. The change in their economic situation during the period was two-fold and can be characterized as follows: First, there was a quantitative increase of women workers, as till then occupations open to women had been limited for a few "new (modern) women" and subsequently became open to the general (ordinary) women. Second, women's employment opportunities were extended to include what were traditionally "men's occupations". In particular, women's participation in professional jobs was enlarged. This was closely linked to the expansion of educational opportunities, which in turn, accelerated their economic participation. Women's greater social participation was, however, only possible on the condition that they do not perform their new roles at the expense of their familial duties. In the circumstances, the traditional notion of "women's place is at home" was not over come ; household chores and child rearing were still women's burden. These were recognized as factors crucial for enhancing women's social activities, and various attempts were made to solve the problem at societal levels, but not in personal or private spheres. Thus these were limited and remained ineffective. In conclusion, the period of US military government in Korea encouraged women to improve their status and change their roles within the new social environment of Liberation and the influx of western culture. This can also to called transitional period, during which traditional patriarchal ideology still dominated over women's life. Although women's enhanced social participation was understood as a "new direction of social progress", it had to be compatible with family life and the ideology of "hyon-mo yang-ch'o" (wise mother and good wife). The latter ideology was maintained as defining the image of the good woman. Thus the period of US military government can be depicted as one of transition, in which traditional values about women altered and new ones emerged.

주의력결핍 과잉행동장애 아동의 연령에 따른 특성비교

정영철,이종범,박형배,정성덕,성형모,사공정규 대한생물치료정신의학회 2001 생물치료정신의학 Vol.7 No.1

Objectives : This study is to verify the developmental changes of ADHD symptoms and functions with aging from 7 to 12 year-old. Method : The subjects of this study were 120 children(108 boys and 12 girls) who were diagnosed as ADHD by DSM - IV at department of neuropsychiatry of Yeungnam University Medical Center. Multidementional tests(K-ABC, ADDES-HV, CAP, CPT, KPl-C, WCST) were carried out to evaluate the change of ADHD symptoms and functions. Results : In K-ABC, children with ADHD showed statistically significant increased "Reading and Understanding" ability(p<.001) with age. In ADDES-HV, They showed decreased inattention (p<.05) and hyperactivity(p<.05) but, not showed significant change of impulsivity symptom with age. In CAP, They showed decreased hyperactivity(p<.001) and inattention (p<.05) and lower total score(p<.05) with age. their result were similar with ADDES-HV. In CPT, They showed decreased hit reaction time(p<.001) and hit reaction time standard errors(p<.01) and variability of standard errors(p<.05) with age. In KPI-C, They showed decreased anxiety(p<.01), depression(p<.001), somatization(p<.05), delinquency(p<.05) and hyperactivity(p<.01) with age. Social relationship(P<.05) was improved, as well as decreased psychoticism(p<.05) and autism (p<.001). In WCST, They showed increased completed categories(p<.001), total corrects(p<.001) and fewer total errors (p<.001) with age. Conclusion : Above findings indicate that symptoms of inattention and hyperactivity in school-aged ADHD children tend to decline with age, but age was less associated with decline in impulsivity symptom . In additions, Their executive functions were improved notably and they appeared stability of emotion, close social relationship and decreased delinquency.

한국 근대사에 있어서 체육에 관한 연구(Ⅱ) : 해방 직후 과도기의 학교체육을 중심으로

이달원,배정행 啓明專門大學 産業開發硏究所 2002 啓明硏究論叢 Vol.20 No.1

본 연구는 해방 직후 과도기 교육과정 속의 체육을 살펴보고자 하였다. 해방 직후 우리나라는 혼란 속에서도 우리 민족선각자들의 민족주의 교육열망과 민주주의에 의한 미군정의 서구적 의식에 의하여 체육이 표면적 발전을 이를 수 있었다. 그러나 당시의 불안정한 시대적 상황과 첨예한 이념의 대치 속에서 그 가치는 혼미를 거듭하였던 것이다. 따라서 현대 체육교육이 바로 서기 위해서는 교육에 대한 완벽한 준비와 교육 본연의 의미를 희석시키는 모든 상황과는 단절할 수 있는 교육의 독립성을 유지하여야 한다는 것이다. This research focuses on physical education of school during the time of transition after liberation. Enthusiasm of old scholar and influence from USA educational pattern, Physical education acquired superficial development. However. Value of physical education under social disorder and ideology conflict were not fully assessed, Preparation and Isolation of education itself are critical to realize true meaning or thought of physical education.

열대지방사람들의 땀샘의 밀도와 기능

이정범,배준상,민영기,양훈모 순천향의학연구소 2004 Journal of Soonchunhyang Medical Science Vol.10 No.1

Tropical inhabitants are able to tolerate heat through permanent residence in the tropics. To clarify the peripheral mechanisms of the suppressed thermal sweating in tropical natives, sweating responses to acetylcholine (ACh), a primary transmitter of the sudomotor innervation, were compared between healthy Korean and African males. ACh was iontophoretically administered on the forearm. Directly activated and axon reflex-mediated sweat responses were evaluated by quantitative sudomotor axon reflex test (QSART). The sweating of axon reflex (nicotinic receptor) onset-time was 0.42 min shorter (P<0.01) and the sweat volumes (muscarinic receptors) were 75% (P<0.01) and the ACh sweat volumes of axon reflex (nicotinic receptor) 79% higher (P<0.01) in the Koreans than the Africans. Iodine-impregnated paper method revealed that sweat gland density was 45.1% higher (P<0.01) and sweat gland output per single gland was 18.4% larger (P<0.001) in the Koreans compared to the Africans. These results indicate that suppressed thermal sweating in Africans is, at least in part, attributed to the suppressed glandular sensitivity to ACh through both recruitment of sweat glands and sweat output per each gland.

기관지 방선균증의 임상 1례

이은영,장태원,정만홍,옥철호,이규원,손창배,이지숙,신은경,서정아,백종현,김영옥 고신대학교의과대학 2007 고신대학교 의과대학 학술지 Vol.22 No.2

Actinomycosis is a chronic suppurative infection, whitch infiltrates mucosa-associated tissues. Thoracic, pelvic and cervicofacial infections of actinomyces are not uncommon, but endobronchial actinomycosis is rarely reported. We report a case of a 29 year old man who presented with a recurrent pneumonia and chronic cough. Fiberoptic bronchoscopy revealed occlusion of the right. lower medio-basal segment bronchus with exophytic endobronchial mass. The diagnosis was confirmed histologically by demonstration of sulfur granules in the bronchoscopic biopsy of the mass. Intravenous administration of amoxacillin and clindamycin followed by oral amoxacillin and clindamycin therapy resulted in complete recovery.