단백질-다당질 복합겔의 용해도 및 전기영동 특성에 미치는 몇가지 첨가제의 영향

정경임,이동선,하정옥 경남대학교 신소재연구소 1996 論文集 Vol.6 No.-

본 연구에서는 닭고기에서 조제한 근원섬유 단백질(MP)과 MP와 유청 단백질 분리물(WPI)의 혼합물, MP와 대두 단백질 분리물(SPI)의 혼합물 등의 3가지 종류의 단백질과 다당질로서 methyl cellulose, sodium alginate, xanthan gum을 택하여 단백질-다당질의 복합겔을 만들고 NaCl, NaSCN, propylene glycol, 2-mercaptoethanol들의 처리에 의해 단백질-다당질간의 상호작용에 기인하는 겔의 용해도 및 전기영동에 의한 겔 용해에 따른 상징액의 변화 등을 조사함으로써 단백질 식품의 기능적 특성에 미치는 영향을 고찰해 보았다. 겔 용해도는 대체로 모든 다당질에 대해 낮은 용해도를 나타냈으나 세 가지 단백질 시료에 대해 2-mercaptoethanol 처리시 가장 많은 단백질이 용해되어 나온 것으로 나타났다. 2-mercaptoethanol은 단백질간 상호작용, 즉 겔 망상구조를 이루는 sdisulfide 결합을 크게 방해함으로써 가장 높은 용해도를 나타낸 것으로 생각된다. 모든 시료에 대해 NaCl과 NaSCN 용액에 대해서는 아주 낮은 용해도를 보였는데, 이것은 NaCl과 NaSCN은 저 농도일 경우, 단백질 구조를 안정화함으로써 단백질간의 상호작용이 크게 증가되어 용해되는 단백질의 양이 극히 낮아진 것으로 보인다. Xanthan gum은 가장 겔 용해도가 낮았고, 특히 MP와 xanthan gum으로 이루어진 겔에서는 아주 극소량의 단백질이 용해되었다. Methyl cellulose 겔의 경우, 모든 용액에서 단백질이 거의 용해되어 나오지 않았다. 그러나, 1% WPI를 혼합한 경우 역시 겔 용해도가 낮았으며 여전히 MP의 단백질 성분은 용해되어 나오지 않았고 WPI의 주요성분인 α-lactalbumin과 β-latcoblobulin이 많이 용해되어 나왔다. 1% SPI을 혼합한 겔은 셀 용해도도 여전히 낮았으며 전기영동 결과에서도 SPI 성분의 단백질 band가 아주 미약하게 나타났다. 이것으로 보아 WPI는 다소 불안정한 겔을 형성하여 용해도를 증가 시키고 반대로 SPI는 겔을 안정화하여 단백질 용해도를 감소시켰으리라 짐작된다. Sodium alginate 겔의 경우, 단백질 용해도는 아주 낮았으나 3 가지 단백질 시료 모두 용해되어 나왔다. 이것으로 sodium alginate는 단백질과 다소 불안정한 겔을 형성한다는 것을 알 수 있다. X무소무 gum 겔에서도 역시 MP와 MP+SPI 혼합겔은 아주 낮은 겔 용해도를 뒷받침하듯 단백질 band를 거의 확인할 수 없었으나 WPI를 혼합한 겔만이 WPI의 주요성분인 α-lactalbumin과 β-latcoblobulin 성분이 용해되어 나왔다. 즉, methyl cellulose와 xsanthan gum 겔의 경우, 근원섬유 단백질은 거의 용해되어 나오지 않았고 유청 단백질 분리물의 성분이 많이 용해되어 나왔으며 대두 단백질 분리물은 단백질과의 결합력이 커 용해된 단백질 성분이 아주 미약했다. Sodium alginate 겔의 경우 모든 단백질 성분이 용해되어 나온 것으로 보아 sodium alginate는 단백질과 결합하여 불안정한 겔을 형성하는 것으로 나타났다. Interactions between polysaccharides and proteins occur widely in biological system. Their interaction in food system often plays a role in determining the functional properties of these systems. The effects of various reagents such as NaCl. NaSCN. propylene glycol and 2-mercaptoethanol on the gel solubility of heat-induced gels of protein and polysaccharide were studied. Protein samples were myofibrillar proteins(MP) extracted from chicken breast muscle and the combinations of MP plus whey protein isolate(WP1) and MP plus soybean protein isolate(SP1). As polysaccharides in preparation of gels, methyl cellulose, sodium alginate and xanthan gum were used. Protein- polysaccharide composite gels were formed by heating at 80 C for 30 min. In the effects of reagents on gel solubility, 2-mercaptoethanol had higher solubility than other reagents. indicating that 2-mercaptoethanol cleaved disulfide bonds in the protein-polysaccharide composite gels. In order to indicate the composition of protein solubilized from gels by the treatment of various reagents such as 0.1 M NaC1. 0.1 M NaSCN. 20% propylene glycol, 0.04 M 2-mercaptoethanol, the solubilized proteins were analyzed by SDS-PAGE. In methyl cellulose and xanthan gum gels. less protein was solubilized by reagents, but MP gels with WPI was solubilized more abundantly than SPI.

MANET 라우팅 프로토콜 QoS 지원서비스의 성능평가에 관한 연구

鄭燦赫,金鉉郁,李光培,姜景仁,朴京培,劉忠烈,吳世德,裵振勝,趙榮泰 明知大學校 産業技術硏究所 2004 産業技術硏究所論文集 Vol.23 No.-

In this paper, we evaluated two main QoS supporting routing protocol on wireless ad hoc network. At presence, wireless mobile communication focuses on how to efficiently support mobility of users more than QoS guarantee. However, in order to satisfy requirement of various applications which have been or will be served, QoS support service between source and destination becoming a very important issue. Of all routing protocols, DSR and AODV are very important routing protocol in MANET. So we simulated DSR and AODV QoS Routing Protocol Through simulation evaluation tool NS(Network Simulation) based on various environments.

수관 보일러의 동특성 해석(Ⅰ) : Modeling of Water Tube Boiler 수관 보일러의 모델링

양경욱,양정규 여수대학교 산업기술지역개발연구소 2001 産業基術硏究所 論文集 Vol.10 No.-

As recent energy-generating devices tend to grow larger and more efficient responding to the demands from users, the mechanical dynamic motion and device design capability. which are critical in making a safe energy-generation device, are highly emphasized. Adequate understanding of dynamic characteristics of an energy-generation device is important in designing the device. Furthermore, it is essential to analyze quantitatively the behaviors of basic designing parameters for managing an energy-generation device efficiently. We have performed a mathematical modeling for each device based on the principle of the conservation of energy to define the thermal and mechanical dynamic characteristics of the combustion chamber, drum, superheater, reheater and economizer, which are components of a boiler.

위험평가(Risk Assessment)에 의한 업종별 위험(Risk)의 구분

박동욱,박덕묵,정광수,윤충식,김태형,노영만,이경남,이송권,김현욱 한국산업위생학회 2003 한국산업보건학회지 Vol.13 No.2

A total of 514 workplaces in 17 industries were inbestigated through walk-through survey. These industries were all small companies with less than five workers and didn't have work environmental measurement data. These industries were ranked by risk that was evaluated by combination of toxicity and possibility of exposure to chemical hazardous agents produced at operations of industry. Risk Index(RI) was qualitatively combined by the combination between hazard and potential of exposure to chemical hazardous agents. Industries that were regarded as having the highest risk were wood and products of wood, chemicals and chemical products, basic metals, other vehicles, trailers and semi-trailers, and fumiture manufacturing. These industries were found to have operations with higher risk than other industries. This study found that more attentions should be paid to these industries with that more attentions should be paid to these industries with high risk.

기종성 신우신염 1례

이재욱,구정태,서정일,양창헌,이정호,이동철,이영현,이활,이경섭 동국대학교 경주대학 1997 東國論集 Vol.16 No.1

기종성 신우신염은 주로 당뇨병환자나 폐쇄성 요로질환을 가진 환자에 발생하며 특징적으로 신실질 및 그 주위조직에 가스를 형성하면서 심한 조직괴사를 일으키는 매우 드문 급성 화농성 신감염으로 사망률이 높은 질환이다. 이 질환은 1989년 Kelly와 MacCallum에 의해 처음 보고된 이래 외국문헌에 약 90례 정도가 보고되어 있으며 국내에는 28례가 보고되어 있다. 수액 및 전해질 보충, 인슐린 투여를 통한 적절한 혈당조절 및 항생제 투여등의 내과적 치료와 절개배농 및 신적출술등의 수술적 치료 방법이 있다. 내과적 치료로 가스의 감소가 없는 경우에는 즉각적인 수술적 치료를 시행하여야하므로 이 가스변화에 대한 추적관찰이 중요하다 하겠다. 본 저자들은 당뇨병환자에서 발생한 기종성 신우신염 1례를 내과적 요법을 치험하였기에 문헌고찰과 함께 보고하고자 한다. Emphysematous pyelonephritis is rare, life-threatening infection of the renal parenchyma and perirenal tisseue. This disease is characterized by the production of intrarenal and perirenal gas and is frequently encountered in patients with diabetes mellitus or urinary obstruction. We experienced a case of emphysematouse pyelonephritis in a 62 years old women with poorly controlled diabetes mellitus who had been managed with medical theraphy. So we report this case with a review of the referenced literatures.

40세 이하 관상동맥 질환자에서 장기적 임상 관찰

이승욱,정명호,박종철,박형욱,조상희,김원,김계훈,이상현,강경태,조장현,김남호,김건형,안영근,조정관,박종춘,강정채 全南大醫大 2001 전남의대학술지 Vol.37 No.2

뚜렷한 입체 선택성을 갖는 알릴 디올의 고리 카보네이트와 아세토니드의 Wacker 산화 반응 연구

강석구,정경윤,정재욱,남궁은영,김태현 성균관대학교 기초과학연구소 1995 論文集 Vol.46 No.1

Palldium(Ⅱ) 촉매 하에서의 말단 알릴 디올의 고리 카보네이트와 아세토니드의 산화반응은 anti-Markovnikov hydation에 의해 알데히드 또는 α,β-불포화 알데히드 만이 생성되었다. 치환된 알릴 디올의 분자내 이중 결합의 반응으로는 (E)-알릴 디올의 경우에 β-케토 생성물을, 반면에 (Z)-알릴디올의 경우엔 α-케토 생성물을 얻을 수 있었다. 치환된 알릴 디올의 고리 카보네이트와 아세토니드에서는 각각 β-케토 화합물과 α,β-케토 화합물 들이 생성되었다. Palladium(Ⅱ)-Catalyzed oxidation of acetonides or cyclic carbonates of terminal allylic diols afforded aldehydes or α,β-unsaturated aldehydes as the sole products, resulting of anti-Markovnikov hydation. For the internal olefins of the substituted allylic diols, (E)-allylic diol provied β-keto-product, whereas (Z)-allylic diol affored α-keto-product. The acetonides and cyclic carbonates of the substituted allylic diols yielded β-keto-products and α,β-unsaturated ketones, respectively.

이동 애드 혹 네트워크에서 QoS를 지원하는 AODV 라우팅 프로토콜

吳世德,趙榮泰,丁燦赫,金玄郁,李光培,姜景仁,朴京培,文泰洙,郭承郁,河載承 明知大學校 産業技術硏究所 2003 産業技術硏究所論文集 Vol.22 No.-

Abstract - In this paper, we propose a bidirectional mobile Ad Hoc routing protocol based on AODV(ad-hoc on-demand distance victor routing) with QoS(quality of service) support. At presence, wireless mobile communication focuses on how to efficiently support mobility of users more than QoS guarantee. However, in order to satisfy requirement of various applications which have been or will be served, QoS guarantee between end point is a very important issue. The existing AODV routing protocol is implemented to use only BE (Best Effort) service. However, actual wireless mobile environment requires to reduce a waste of communication resources and meet with the real-time change of data paths by setting up routes with QoS support, considering available communication resources at each node in advance. Therefore, in this thesis, we propose an adaptive QoS support method using hello message in order to rapidly meet with the change of available communication resources and to smoothly perform route maintenance. For performance evaluation, we analyzed the average date reception rate. At that time, we used evaluation parameters such as node's mobility -stop time, hello message period, and packet priority, considering the node's mobility and the number of best effort/QoS data flows. As results, we found that with our proposed method the average data reception rate was increased 16.3% for 0 mobility-stop second case and 4.7% for 300 mobility-stop second case.

팔체질침을 이용한 반월판 관절경부분절제술 환자의 치험례

강영화,김성욱,손인석,서정철,윤현민,장경전 동의대학교 한의학연구소 2001 동의한의연구 Vol.5 No.-

슬관절은 지속적인 압력을 받거나 충격때문에 동작범위의 한계에 이를 때 손상받기 쉽다. 반월판 파열 환자는 일상생활에 지장을 주는 동통, 정복되지 않는 잠김, locking이나 giving way가 자주 일어나는 경우, 지속적인 신전제한이 있을 때 수술의 적응증이 된다. 반월판 파열 환자의 재활에 있어서 관건은 대퇴사두근의 근력과 ROM의 회복이다. 팔체질침 시술후 반월판 부분절제술을 시행한 환자가 동통의 강도와 동통 재발의 감소, ROM의 개선, 전반적 상태면에서 체침이나 MPS 시술 때보다 더 나은 경과를 얻었다. Knee joints are apt to be injured when they are over the limit of ROM by continuous pressure or impact. The patient of meniscus tear has to be operated in case that he has the symptom of knee pain obstructive to daily life, unreducable locking, frequent locking or giving way, continuous extention limit. In the rehabilitation of the patient of meniscus tear, the key is the progress of musculi quadratus femori's power and ROM. After 8 constitutional acupucture therapies, partial menisectomy patient has the better process about decrease of pain intensity and relapsing pain frequence, development of ROM, general condition more than general acupucture or MPS medical treatment.

그람양성구균에 대한 Teicoplanin과 Vancomycin의 시험관내 항균력

최태열,김경숙,전용관,서일혜,김정욱,이웅수,안정열,김홍석,정재용,최효선,김덕언,유진우 대한감염학회 1994 감염 Vol.26 No.1

An increasing frequency of methicillin resistant S. aureus(MRSA), methicillin resistant coagulase negative staphylococci(MRCNS) and Enterococcal infection have been observed in recent years. Teicoplanin is a new glycopeptide antibiotic obstained from the Actinoplanes teicomycetius. The molecular structure and spectrum of antimicrobial activity of teicoplanin is simillar to those of vancomycin, and has been reported to have an excellent in vitro and in vivo effect against various gram-positive infections. Therefore, we evaluated the in vitor susceptibility of gram positive cocci, such as, S. aureus, coagulase negative Staphylococci(CNS), and Enterococci to teicoplanin and vancomycin. The total 253 strains consisted of MSSA(40), MRSA(53), MSCNS(47), MRCNS(48), and Enterococci(65). They were assayed by disc diffusion and agar dilution. During the study, 57% of S. aureus and 49% of CNS showed resistance to methicillin. The inhibitory diameter of teicoplanin was 15-20mm in MSSA, 12-19mm in MRSA, 13-24mm in MSCNS, 11-23mm in MRCNS, and 15-22mm in Enterococci respectively, and showed sensitivity in all but 8 strains(3.2%). The range of the minimum inhibitory concentration (MIC) of teicoplanin to MSSA, MRSA, MSCNS, MRCNS and Enterococci were 9.12-2.0㎍/ml, 0.25-2.0㎍/ml, & 0.25-32㎍/ml, 0.12-1.0㎍/ml respectively. One case of S. haemolyticus was resistant to teicoplanin (32㎍/ml) by the agar dilution method. Eight minor (3.2%) and one major(0.4%) error was observed when the MIC and disk diffusion data were correlated with teicoplanin. As for vancomycin the inhibitory diameter was 17-21mm in MSSA, 15-21mm in MRSA, 18-26mm in MSCNS, 18-25mm in MRCNS, and 16-22mm in Enterococci respectively. The range of the MIC of vancomycin to MSSA, MRSA, MSCNS, MRCNS, and Enterococci were 0.25-1.0㎍/ml, 0.25-4.0㎍/ml, 0.5-2.0㎍/ml and 0.5-2.0㎍/ml respectively. One minor error (0.4%) was seen with the vancomycin disk. The MIC90 of MSSA and MRSA exhibited the same results in teicoplanin (1.0㎍/ml, 1.0㎍/ml), and vancomycin(2.0㎍/ml, 2.0㎍/ml). MSCNS and MRCNS exhibited greater MIC90 with teicoplanin(4.0㎍/ml, 8.0㎍/ml) than vancomycin(2.0㎍/ml, 2.0㎍/ml). Incontrase Enterococci were more susceptible to teicoplanin(0.5㎍/ml) than to vancomucin (2.0㎍/ml). Results from this analysis indicated that both teicoplanin and vancomycin were very excellent for gram positive infections, especially those resistant to methicillin.