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RAW264.7 세포에서 interferon-r 및 LPS에 의해 유도되는 NO생성에 미치는 TALT-35의 영향
박종일,박경석,김종석,박지훈,윤은진,송경섭,서강식,김훈,윤완희,박승길,임규,황병두 충남대학교 생물공학연구소 2006 생물공학연구지 Vol.12 No.-
TALP-35 purified from human term placenta is known to increase microtubule polymerization and stabilize the polymerized microtubule. To examine the effect of TALP-35 on immune system this study was performed. MTT assay was performed to investigate the effect of TALP-35 on the proliferation of RAW264.7 cells. TALP-35 dose dependently suppress the proliferation of RAW264.7 cells at high concentration (above 1 μM) in unstimulated cells, in case of 10 μM TALP-35 treated cells the suppression was 25% but in stimulated cells it was only 15%. Cosedimentation assay was carried out to investigate whether TALP-35 can bind to tubulin of RAW264.7, monocyte/macrophage lineage of mouse, and polymerize it. TALP-35 polymerize the tubulin of RAW264.7 cells and sedimented in dose-dependent manner. To investigate the effect of TALP-35 on the expression of iNOS protein western blotting was performed. The expression level of iNOS was decreased dose dependently in high concentration of TALP-35 treatment. To examine the activity of iNOS, secreted NO was determined by method based on Griess reaction. Interferon-γ and LPS-stimulated production of NO from RAW264.7 cells was decreased dose dependently above 0.1 μM concentration of TALP-35 and 50% is decreased at 10μM of it. This study shows TALP-35 can control cytokine induced-iNOS expression therefore it might control inflammatory diseases.
Antiadhesive effect and safety of oxidized regenerated cellulose after thyroidectomy
Kyoung Sik Park,Kyu Eun Lee,Do Hoon Ku,Su-Jin Kim,Won Seo Park2,Hoon Yub Kim3,,Mi Ra Kwon1,Yeo-Kyu Youn1 대한외과학회 2013 Annals of Surgical Treatment and Research(ASRT) Vol.84 No.6
Purpose: To evaluate the antiadhesive effects and safety of an oxidized regenerated cellulose (Interceed) after thyroidectomy. Methods: Seventy-six thyroidectomized patients were prospectively randomized into two groups with regard to the use of Interceed. We evaluated each group for their adhesive symptoms using four subjective and four objective items at the 2nd week, 3rd and 6th month after thyroidectomy. All patients were examined for vocal cord motility by indirect laryngoscope at each period. Results: Total adhesion scores at each postoperative follow-up period decreased with time, but were not significantly different in each group. The median score for swallowing discomfort for liquid was significantly lower in the Interceed group than in the control group 2 weeks after surgery. In addition, the severity of skin adhesion to the trachea was reduced in the Interceed group compared with the control group 6 months after surgery. During the study, there were no adverse effects or significant differences in postoperative complications between the groups. Conclusion: Interceed appeared to be safe and effective in improving neck discomfort at early postoperative periods and preventing skin adhesion to the trachea 6 months after thyroidectomy.
순간기화기를 채용한 다단 압축 냉동 사이틀에서 최적의 중간 압력
박영선,김경훈 國立金烏工科大學校附設生産技術硏究所 1997 産業技術開發硏究 Vol.13 No.-
This paper presents the analytic prediction of the refrigeration cycle with flash tank and intercooler. The removal of flash gas developed in the throttling process between the condenser and the evaporator improves the Coefficient of Performance of the refrigeration cycle. Intercooling also improves the Coefficient of Performance of the refrigeration cycle as saving in the compressive work. For this analysis, each of eleven refrigerants was used. The effects of evaporating and condensing temperature on the optimal intermediate pressures were investigated, and compared with the theoretical pressure of intercooler.
박금수,김영식,이진응,임종천,이강현,임경수,황성오,최경훈 대한응급의학회 1995 대한응급의학회지 Vol.6 No.2
To estimate the quality of the emergency medical services system of Wonju City, we studied the diurnal variations of 179 non-traumatic cardiac arrest victims who received cardiopulmonary reuscitation at the emergency center of Wonju Christian Hospital. Diurnal variations of non-traumatic cardiac arrest patients were as follows ; The occurence of cardiac arrest at day-time was higher than night-time; 18 cases (11%) from midnight to AM 4, 25 cases (14%) from AM 4 to AM 8, 42 cases (24%) from AM 8 to AM 12, 46 cases (25%) from AM 12 to PM 4, 35 cases (19%) from PM 4 to PM 8, 13 cases (7%) from PM 8 to midnight. Witness cardiac arrest was increased more during the day than night ; 40% from midnight to AM 4, 48% from AM 4 to AM 8, 57% from AM 8 to AM 12.52% from AM 12 to PM 4, 60% from PM 4 to PM 8, 38% from PM 8 to midnight. The transportation time at night-time cardiac arrest was more longer than day-time cardiac arrest ; 30±12mins from midnight to AM 4, 26±9mins from AM 4 to AM 8, 27±12mins AM 8 to Am 12, 25±11mins from AM 12 to PM 4, 25±9mins from PM 4 to PM 8, 35±15mins from PM 8 to midnight. The rate of restoration of spontaneous circulation(ROSC) in day-time cardiac arrest was higher than the night-time cardiac arrest ; 30% from midnight to AM 4, 36% from AM 4 to AM 8, 32% AM 8 to AM 12, 44% from AM 12 to PM 4, 41% from PM 4 to PM 8, 15% from PM 8 to midnight. The survival rate of cardiac arrest has been correlated with collapse time, early bystander CPR, early advanced care. To improve outcome for prehospital cardiac arrest, we concluded that early bystander CPR, and early advanced life support should be performed at the scene and during the transportation especially at night.
박경수,최훈,이정렬,엄진섭 강원대학교 정보통신연구소 2005 정보통신논문지 Vol.9 No.-
In this paper, we propose a new WDM wavelength locking system which uses the Michelson interferometer with a movable arm. The proposed system can cope easily with an external temperature variation to give a good stability and shows the wavelength locking capability of a resolution below 0.0005㎚.
박혜연,김종훈,박경일,황철웅,김태년,남궁준,도준형,이원로,이성윤 白中央醫療院 2005 仁濟醫學 Vol.26 No.1
Brugada syndrome is characterized by right bundle branch block morphology and ST segment elevation in the right precordial leads and a propensity to develop ventricular arrhythmias. Mutations in a cardiac sodium channel gene have been linked to this syndrome, and the ionic mechanisms responsible for the electrocardiographic phenotype are temperature-dependent. This case report describes a patient in whom a typical Brugada ECG pattern developed during fever and returned normal ECG after fever was subsided.
최훈,박경수,이정렬,엄진섭 江原大學校 産業技術硏究所 2004 産業技術硏究 Vol.24 No.A
In this paper, we proposed an optical balance using a Michelson Interferometer structure. The frequency of the interference signal is changed nonlinearly related with a weight to be measured, and this frequency change is processed by a processor to give a measured weight. Several measurements were performed and the good results were obtained with the fluctuation of about 1g.
高等學校 排球選手에 있어서 스포츠傷害의 心理的 要因硏究
민경훈,송채훈,박종남 서강정보대학 1993 論文集 Vol.12 No.-
This study examines the relationship between psychological factors and sport injuries in high school volleyball player and offeris data to improve concentration of team's power. The subjects have collected from 126 high school volleyball players(male:72,female:54) participating The Third Mooduying Cup High School Champianship Volleyball Contest. The factors of this study are A type behavior, locus of control,life event,history of injury, and social support. The results are as follows; 1. Most of all experience three sport injuries, are state of "moderate crisis, and male have more than female in history of injuries. 2. A type behavior, history of injury, and life event scale have an effect to take place sport injury in all players. But other factors are not related to sport injury.
아파트 프로젝트의 시공성 실무적용방안 분석에 관한 연구
한경훈,김문규,박찬식 대한건축학회 2004 대한건축학회 학술발표대회 논문집 - 계획계/구조계 Vol.24 No.1
Process of construction project is made of plan, design, procurement, construction, and maintenance, which are closely related each other. for the success of project, each step of process should maintain the interface. The concept of constructability appeared in the practical business of an advanced country to maximize efficient integration. However, the concept of constructability in our country is not used in overall process to optimize each phase. To proceed the study, first, impact factors of constructability were identified, and had an interview with professionals. As a result, complexity of design in design phase, and responsibility of the contractors in construction phase are analyzed as the most important thing. Based on this result, I made secondary survey to identify the facts needs to be considered to applicate the analysis on the practical business. As the result of secondary survey, the ranking of considerations for application of practical business was identified. Therefore, this study suggested the basic direction to the applications of constructability.