독성물질의 세포사 기전 및 세포사 유발물질의 검색법 개발에 관한 연구(Ⅰ) : 독성물질로 인한 파킨슨병 모델에서의 세포사 기전 연구 Study on the cell-death mechanisms of toxin-induced parkinsonism

강태석,김종민,서경원,김영옥,김준규,오재호,이윤동,김규봉,오정자,송연정,임종준,전범석,문전옥,최광식 식품의약품안전청 2000 식품의약품안전청 연보 Vol.4 No.-

MPTP 독성물질이 도파민성 신경세포에 선택적으로 작용하여 산화성 손상에 의한 신경세포사를 일으키는 것을 이용하여 파킨슨병의 동물모델을 만들고, 이를 통해서 아폼토시스를 비롯한 포사의 기전에 대한 연구 및 너코틴의 신경세포 보호효과 여부를 판정하는 실험을 병행하고자 하였다. 파킨슨꾐의 동물모델을 MPTf 독성 물질을 이용하여 확립하였으며, MPTP(30mgag, i.p.)를 투여한 후 1, 2,3, 4, 5일째 흑질 조직을 채춰하여 tarm로 박걸하여 tyrosine hydroxylase 면역조직화학염색을 수행하여 cell countif우한 결과, control은 57.635ce11s, 1일째 친.OfDells,2일째 57.9±6cells,3일릴 없.3±죠ells, 4일째 49.0츠3cells, 5일째 39.4±Scells료 4, 3일째 뚜렷한 신경세포 수의 감소를 보였다. 신경세포사 기전 규명을 위한 아폼토시스 분걱에서는 벼PTP 투여 후 1, 2, 3, 4, 5일째 조직을 채취하여 Hoechst staining, TUNEL staining을 수곡하였는데 양성 반응을 보인 신경세포는 관찰되지 않아. 아폼토시스로 인한 세포사가 관찰되지 않았다. bIPTP 파킨슨병 동물모델에서 nicotine 보호효과 탐색에 관한 실험은 nicat푸e 0.2mgAg을 5일 퐁안 투여 후 리『fP(30mgag)를 CS7Bt/6 마은스에 복강 내주사로 nicotine과 병용 투여한 후 1, 2, 3, 4, 5일째 뇌를 적출하땄다. 신경세포사가 뚜렷이 관찰되기 시작하는 4, 5일째의 신경세포 수의 감소 정도를 20. 30% 정도 약화시키는 경향을 보였으나, nicotine 보호효과에 대한 추가 실헝이 현재 수행 중에 있다. The cause of Parkinson's disease (PD) is largely unknown. However, free radical toxicit? may plaf a role ip. the degeneration of substantia nigra, which is the Hajorfocus of pathological damages in PD. Recently, a neuroprotective effect of nicotine in PD has been suggested. Therefore, the mechanism of neurodegenerafion and protective potential o( nicotine in PD were investigated in the experimental modeB of Pll using a neurotoxin, C57BL/6mice were administered with 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP, 30 mg/kg,j.p.). The degree of neurodegenerafion was determined by immunohistochemical stainiHB oftyrosine hydroxylase (TH). TH-positive cells on nigral sections were found 56.0 ±4, 57.9 ±6,52.315ce11s, 49.0±3cells, and 39,4±Scells at days 1, 2, 3, 4, 5, respectively (controls : 57.6±Scells). Hoechst and TUNEL staining showed no evidence of apoptosis. The exandnation on themice co-adrunistered with nicotine(0.2mgAg) and MPTP(30mgag) revealed a tendency ofnicotine protective effects. At days 4 and 5, the degree of TH-positive cells was decreased by20-30%, In corclusiffn, the role of apoptosis was not evidenced in this MPTP modeB of PB.The possible proteccon by nicotine should be elucidated with further studies.

Gear Pump의 問題點

남궁석,權敬祐 大田工業高等專門學校 1969 論文集 Vol.4 No.-

Gear pump, having been studied and improved carefully in every respect these years, began to have a rapid high speedness without many defects that existed before. Therefore, today it can be operated with more than 100 ㎏/㎠ of high pressure. In this reseach, the important problems of gear pump are mentioned : 1. During the operation, to control the increase and decrease of the unusual pressure between the gears, and to produce better efficiency. 2. During the operation, to examine the weight and direction of the shaft load and to select the proper bearing to it. 3. To select the ratio between the outer diameter of gear and the width of gear. 4. To select the number of gear teeth. 5. The design of clearance.

오픈프라이스 제도에 대한 소비자인식도 및 만족도

김인석, 서경원, 유현정 충북대학교 생활과학연구소 2013 생활과학연구논총 Vol.17 No.2

The purpose of this study was to analyze consumers' awareness and satisfaction of open price system. Questionnaire survey was conducted from 3 September to 14 September 2011. We have surveyed 200 consumers who were living in Cheongju area. Major findings are as follows. First, consumers were not aware about open price system very well. The mean of the objectives and expected effects of open price system was 2.38. It means that many consumers when it comes to the open price system does not have the proper recognition was considered. Second, there was not big changes for consumers' information search behavior after execution the open price system. Third, consumers' satisfaction mean(m=2.32) of open price system was lower than dissatisfaction mean(m=3.40) of that. The main reason of dissatisfaction due to inconsistent pricing confusion was investigated. Fourth, there were statistic differences in open price system awareness and consumer behavior by consumers' socio-economics characteristics such as age, education, income, occupation, etc. Finally, consumers' satisfaction of open price system was influence by information search cost, product or brand image. On the other hand, consumers' dissatisfaction was only influence by open price system awareness.

한국현대소설의 일본 인식

서경석 우리말글학회 2002 우리말 글 Vol.24 No.-

This study aims at the understanding of japan and japenese effect presented in the Korean Modern Nevels. Chaper 2 makes clear the Meaning of the recognition of foreign countries as the outside. Chapter 3 studies the aspects of recognition about japan in <Sinsoseol> and Chapter 4 & 5 analyze the japanese effect in the literary works of Song Young and Yeom, Sang-seop.(Hanyang University)

김남천의 <발자크 연구노트>론

서경석 대구대학교 인문과학 예술문화연구소 1999 人文科學硏究 Vol.19 No.-

한국경향소설의 근대성 비판

서경석 대구대학교 인문과학연구소 1996 人文科學硏究 Vol.15 No.-

만주국 기행문학 연구

서경석 한국어문학회 2004 語文學 Vol.0 No.86

The main purpose of this thesis is to arrange travel description to Manchuria and explain the meaning of that. There were lots of Korean writers to travel to Manchuria in 1940s' period. And they wrrote the account of trip, wrote the novel arelated to that subject. But this trip was compulsed by Japanese imperialism. Therefore they were putted to the test on the pro-japanese or not. It is the point of this paper what the result of the test was. As the conclusion, three types is represented. Firstly, the type of Jung, In-taek is extracted. Secondly, that of Lee, Gi-yong, thirdly, Han, Sel-ya. And each type has the different recognition about Manchuria and the relation between Korean and chinese.

폴리카보네이트 브라켓 부착 시 결합강도 증진을 위한 표면처리 효과

김석필,김년경,이현정,황현식 대한치과교정학회 2006 대한치과교정학회지 Vol.36 No.5

본 연구는 폴리카보네이트 브라켓 부착 시 표면처리가 결합강도를 증진시키는지 알아보고자 시행되었다. 소의 하악 중절치를 포매하여 만든 100개의 시편에 광중합형 레진 접착제를 이용하여 폴리카보네이트 브라켓(Alice, 광명 데이콤, 대한민국)을 부착하였다. 대조군의 경우, 표면처리 없이 부착한 반면, 실험군의 경우 샌드블라스팅 처리한 경우, plastic conditioner 처리하여 부착한 경우, 그리고 샌드블라스팅과 함께 plastic conditioner 처리한 경우로 구분하여 접착하였다. 만능시험기를 이용하여 전단결합강도를 측정하고 파절양상을 비교 분석한 결과, plastic conditioner나 샌드블라스팅으로 표면처리를 시행한 경우 표면처리를 시행하지 않은 경우에 비해 높은 결합강도를 보였다 (p<0.001). 샌드블라스팅과 Plastic conditioner를 모두 처리한 경우는 샌드블라스팅만이나 plastic conditioner만 처리한 경우보다 높은 결합강도를 보였으며, 특히 샌드블라스팅만 처리한 경우에 비해서는 통계적 유의차를 보였다 (p<0.05). 이상의 연구결과는 폴리카보네이트 브라켓 부착 시 결합강도 증진을 위해서 베이스의 표면처리가 필요하며 샌드블라스팅 후 plastic conditioner 도포가 가장 효과적임을 보여주었다. Objective: The purpose of this study was to evaluate whether the bond strength of polycarbonate brackets can be increased through surface treatment. Methods: One hundred polycarbonate brackets (Alice) were bonded to bovine incisors with light-cured adhesive. The bracket bases were treated with one of three methods; sandblasting, plastic conditioner application, and combined treatment with sandblasting and plastic conditioner. The brackets without any surface treatment served as the control. The shear bond strength was tested with a universal testing machine, and failure pattern was assessed with the adhesive remnant index. Results: The shear bond strength in all experimental groups was higher than that of the control group (p<0.001). The group treated with plastic conditioner after sandblasting showed statistically higher shear bond strength than the sandblasting only group (p<0.05). The group treated with plastic conditioner after sandblasting showed higher shear bond strength than plastic conditioner only group, but the difference was not statistically significant. Conclusion: The above results suggest that the surface treatments of polycarbonate bracket is mandatory to improve bond strength, and the most effective method is an application of plastic conditioner after sandblasting.

응급의료센터 과밀화 해소를 위한 환자 전원

이경룡,구홍두,박인철,김승환,장석준,심호식 대한응급의학회 1995 대한응급의학회지 Vol.6 No.2

To reduce the degree of overcrowding in level Ⅲ emergency care centers, authors have conducted a prospective study of patient who were transferred to level Ⅰ or Ⅲemergency center based on the non-emergent patient guideline currently used at the severance Hospital, level Ⅲ emergency center, during the period of 65 days from Jan. 1st 1995 to Mar. 6th 1995. Followings are guideline for non-emergent patient described by the Korean government low: 1) systolic blood pressure greater than 80/100 of normal. 2) respiration rate between 10 to 24 per minute. 3) pulse rate between 60 to 100 per minute. 4) body temperature between 36 to 37.5℃ by rectal. 5) relatively alert mental status. 6) patient not requiring emergency operation at the time of examination. The results were as follows: 1. Among the total 5,301 patients, 464 patients ranged from age of 1 to 82 years old were transferred to level Ⅰor Ⅱ emergency center after simple treatment. 2. Among the 464 patients, 276 patients were treated in expected hospital with medical problems in 101 (36.6%) patients, and 175 (63.4%) patients had surgical problems. 3. Among the 276 patients, 122 (44.2%) patients were admitted, 145 (52.9%) patients were discharged from the emergency room after adequate treatment. 4. Among the transferred patients, simple laceration (33.0%) were most common, acute gastroenteritis (12.0%), and simple contusion (10.5%) were followed. These results suggest that about 10% of patients visiting level Ⅲ emergency care center, can be safely transferred to nearest level Ⅰor Ⅱ emergency centers on the bases of non-emergent patient guideline described by Emergency Medical Service law. These guideline, if put into proper use, can reduce the degree of overcrowding problems in level Ⅲ emergency care center through out the country.

폴리머 모르터의 난연성 개선에 관한 연구

연규석,大濱嘉彦,김남길,김철영,지경용 강원대학교 부설 석재복합신소재 제품연구센터 2000 석재연 논문집 Vol.5 No.-

불포화 에스트테르 수지의 큰 이용분야의 하나로 건축재료가 있으나 여기서 가장 문제로 되어 있는 것이 바로 난연성의 향상이다 불포화 폴리에스터수지는 탄소를 구성성분으로 하는 유기물이므로 무기재료와 같은 불연성을 기대하기는 어려우나 어느 정도까지는 연소를 억제시킬 수는 있다. 본 연구에서는 UP를 결합재로 하고 탄산칼슘을 충진재로, 규사를 잔골재로 하는 폴리머 모르터를 제조하고 이에 첨가되는 난연제량과 UP량을 각각, 5~12.5%, 12~24%로 변화시켜 난수별 난연특성을 고찰하였던 바, UP사용량에 따른 특정 난연제의 최적 사용량 범위를 얻을 수 있었다. One of the fields using the unsaturated polyester resin is the constructional material, but the most serious problem, in this field, is the incombustibility improvement. it is hard to expect the noncombustibility as in the inorganic materials for the unsaturated polyester resin since whose structural material is carbon, but the combustion can be controlled up to some degree. In this study the polymer mortars were made in which the unsaturated polyester resin as binder, the calcium carbonate as filler and the silica sand as fine aggregate. The incombustible characteristics were then investigated as varying the amount of the flame retarder and the unsaturated polyester resin from 5 to 12.5% and 12 to 24%, respectively. Based on the results, the optimized range of amount of flame retarder used with various amount of unsaturated polyester resin could be obtained.