Interleukin-1B(1L-1B) polymorphisms and gastric mucosal levels of IL-Iβ cytokine in Korean patients with gastric cancer

Chang, Young-Woon,Jang, Jae-Young,Kim, Nam-Hoon,Lee, Jae Won,Lee, Hyo Jung,Jung, Woon Won,Dong, Seok-Ho,Kim, Hyo-Jong,Kim, Byung-Ho,Lee, Joung-Il,Rin Chang KYUNG HEE UNIVERSITY MEDICAL CENTER 2006 고황의학상 수상논문집 Vol.21-22 No.-

Interleukin-1B and IL-1 receptor antagonist gene polymorphisms are associated with an increased risk of gastric cancer (GC) in Caucasian populations. However, recent studies could not find any association between IL-1B-511T polymorphism and the risk of GC in Asians. We tested for an association between IL-1 loci polymorphisms with increased gastric mucosal levels of IL-1β and an increased risk of developing GC in a Korean population. Polymorphisms of IL-1A-889, IL-1B-31, IL-1B-511 and IL-1RN were genotyped in 434 controls and 234 patients with GC. Mucosal IL-1β cytokine was measured using an ELISA. The frequencies of IL-1A, IL-1B-511, IL-1B-31 and IL-1RN were not statistically different between controls and all patients with GC. After subclassification of GC, only patients with intestinal-type GC showed a higher frequency of IL-1B-31T homozygotes (OR = 2.2; 95% CI = 1.1-4.3) compared with controls. Risk was also significantly increased in these patients for IL-1B-31T homozygotes compared with patients with diffuse-type GC (OR = 3.4; 95% CI = 1.5-7.7). As in Caucasian populations, linkage disequilibrium between IL-1B-31 and IL-1B-511 was nearly complete, but the pattern of haplotype related to the risk of GC (IL-1B-31T/IL-1B-511C) was opposite (IL-1B-511T/IL-1B-31C). Mucosal IL-1β levels in H. pylori-infected GC patients were higher in patients homozygous for IL-1B-31T compared with IL-1B-31C/T and IL-1B-31C/C. Thus, the combined effects of H. pylori infection and IL-1B-31T/IL-1B-511C polymorphisms with enhanced mucosal IL-1β production contributed to the development of intestinal-type GC in this Korean population.

특발성 염증성 근육병증 환자에서 IL-17 발현의 증가

백승훈 ( Seung Hoon Baek ),이준희 ( Jun Hee Lee ),김근태 ( Geun Tae Kim ),이정욱 ( Joung Wook Lee ),조미라 ( Mi Ra Cho ),김주인 ( Ju In Kim ),이선희 ( Sun Hee Lee ),김대성 ( Dae Seong Kim ),김성일 ( Sung Il Kim ) 대한류마티스학회 2008 대한류마티스학회지 Vol.15 No.2

Objective: Idiopathic inflammatory myopathies (IIMs) are systemic autoimmune diseases characterized by infiltration of T lymphocytes, monocytes, and macrophages in muscle tissues. Interleukin-17 (IL-17), a Th17 cytokine, has potent pro-inflammatory actions and plays a role in autoimmune diseases. We investigated the expression of IL-17 in muscle tissues of patients with IIMs. Methods: We measured the IL-17 mRNA level of muscle tissues from 14 patients with IIMs (9 patients with dermatomyositis and 5 patients with polymyositis) by real-time RT-PCR and compared with controls. We also performed an immunohistochemical stain to detect IL-17 expression. Results: The expressions of IL-17 were significantly enhanced in IIMs than controls. In immunohistochemistry, IL-17 was expressed in perimysial, endomysial and perivascular infiltrating inflammatory cells. Conclusion: These results suggest that IL-17 plays a role in the immunopathogenesis of IIMs.

Analysis of histone deacetylase inhibitor-induced responses in human periodontal ligament fibroblasts.

Kim, Tae-Il,Han, Ji-Eun,Jung, Hong-Moon,Oh, Joung-Hwan,Woo, Kyung Mi Kluwer Academic Publishers 2013 Biotechnology letters Vol.35 No.1

<P>Periodontal ligament (PDL) fibroblasts play critical roles in the regeneration of periodontal tissues damaged by periodontitis. Histone deacetylase inhibitors (HDIs) have been suggested to be potential tools in tissue engineering. The feasibility of using the HDI, sodium butyrate (NaB) for periodontal regeneration was examined by evaluating its effect on the osteogenic differentiation of human PDL fibroblasts and its modulation of the inflammatory responses to lipopolysaccharide (LPS). NaB did not cause significant cell death at 100?μM but promoted the expression of the osteoblast phenotype (Runx2, osterix, osteocalcin, and bone sialoprotein). NaB significantly inhibited the LPS-induced production of reactive oxygen species and the expression of pro-inflammatory cytokines (IL-1β and TNF-α). These results suggest that HDIs can offer a potential therapeutic agent for periodontal regeneration.</P>

간장 ( 肝腸 ) 및 담도 ( 膽道 ) : B형 활동성 간염 환자에서 단기 Prednisolone과 ARA - AMP 겸용 치료의 경과에 따른 면역 지표의 변화에 관한 연구

민영일(Young Il Min),장린(Rin Chang),김병호(Byung Ho Kim),장영운(Young Woon Chang),이정일(Joung Il Lee),황이숙(Yi Sook Hwang),김정원(Jeong Won Kim),김효종(Hyo Jong Kim) 대한소화기학회 1990 대한소화기학회지 Vol.22 No.3

N/A Withdrawal of corticosteroids in patients with chronic type B hepatitis is frequently associated with enhanced cellular immune response to hepatitis B virus. This immunolgic rebound generally results in a transient reduction in levels of HBV-DNA and DNA polymerase, and seldom results in clearance of HBeAg. There was a pilot study that the combination of a short course of prednisone followed by ARA-AMP was potentially synergistic. The object of this study was to determine the effect of short term prednisolone followed by ARA-AMP on the immune system in patients with chronic active ltepatitis-B. Patients were started 40 mg of prednisolone daily for 4 weeks. After prednisolone therapy was discontinued, patients received no medication for 2 weeks. ARA-AMP was then given for 4 weeks at a dose of 500 mg daily. Immune parameters such as T cell subsets, IL, production, NK cell activeity, and LAK cell activity were tested before and during treatement period. The resullts were as follows. 1) Basal IL2 production in CAH-B (71.3 unit/ml) was lower than that of normal control (p<0.025). Basal NK activity, T4/T8 ratio and LAK activity of CAH-B were similar to those of normal control. 2) After prednisolone treatment, IL2 production, NK cell activity and LAK cell activity were significantly lower than those of basal value. 3) After induction of immune rebound, IL, production increased to 84.8 unit/ml (basal 71.3 unit/ml,

말라리아 환자 32예의 임상적 고찰

정용희,백일현,김태형,김영식,이우인,서환조 대한화학요법학회 2000 대한화학요법학회지 Vol.18 No.1

목적 : 삼일열 말라리아는 국내에서 1986년 이후에는 박멸된 것으로 알려졌으나 1993년 이후 증가하는 양상을 보이고 있다. 이에 말라리아의 임상적 고찰을 통하여 이 질환을 이해함에 있다. 방법 : 1991년 1월부터 1999년 10월까지 반복적 고열과 오한을 주소로 본 병원에 내원하여 말초혈액 도말검사에 의해 말라리아 감염으로 확진된 32예의 환자에 대해서 후향적으로 조사하였다. 결과 : 1991년부터 1999년 10월까지 총 32명의 환자의 연도별 발생빈도는 각각 1991년에 1명, 1996년에 1명, 1997년에 3명, 1998년에 13명, 1999년에 14명이었다. 월별발생빈도는 7월과 8월에 집중적으로 발생하였다.(46.9%) 환자의 거주지는 경기 연천과 파주에 주로 분포되었다(53.1%). 성별발생분포는 32예의 환자중에 여자가 5명, 남자가 27명으로 남자가 더 많았다. 발병연령은 22세에서 87세까지 분포되었고 20-30대에 많이 발생하였으며 20대가 가장 많았다. 군인 및 제대자가 17명(59.4%)에 해당하였으며, 제대자의 평균 제대후의 기간은 10개월 정도였다. 가장 흔한 임상증상은 발열 및 오한이었다.(100%) 비장종대 및 간장종대는 각각 26명(81.3%), 3명(9.4%)에서 관찰되었다. 검사실 소견은 평균적으로 WBC 5.317/㎕, 혈색소 12.5±7.1g/㎗, 혈소판 78,906.3±68391.4㎕, GOT/GPT 43.7±26.7/49.6±57.3 IU/ℓ, Total bilirubin 1.6±0.9㎎/㎗를 보였다. 말초혈액 도말검사상 모두 P. vivax였다. 치료는 hydroxychloroquine과 primaquine의 치료에 잘 반응하였다. 결론 : 말라리아는 7월과 8월에 한국에서 호발하는 중요한 급성 열성 질환으로 조기 진단 및 치료에 보다 관심을 기울여야 할 것이다. Background : In Korea malaria has been rare, but since reemergence of indigenous vivax malaria in 1993, the number of cases of malaria have recently increased. We analyzed the cases of malaria who were treated in Kyung-Hee University College of Medicine with regard to define the clinical characteristics of malaria. Methods : We retrospectively analyzed of 32 cases of malaria admitted to Kyung-Hee University Hospital between 1991 and 1999. Malaria was diagnosed by peripheral blood smear. Results : Among a total of 32 cases, the number of yearly incidence of 1991, 1996. 1997, 1998 and 1999 were 1,1,3,13,14 respectively. It demonstrated the high incidence especially between July and August. The most patients (53.1%) resided in Yeonchon and Pajoo. Among them, 5 cases were female and 27 cases were male. The age distribution ranged from 22 to 76 year old. The high incidence of age was between 20's and 30's. Military solidiers and ex-soldiers are 17 cases(59.4 %). The most frequent symptom was fever and chills (100%). Splenomegaly and hepatomegaly were also observed in 81.3 %(26 of 32) and 9.4 %(3 of 32) respectively. The laboratory findings were WBC 5.317/㎕. Hb 12.5±2.1 g/dl. platelet 78,906±68,391.4/㎕. GOT/GPT 43.7±26.7/49.6±57.3 IU/L. LD 678.3±50.1 IU/L. total bilirubin 1.6±0.9mg/dl- The all cases were treated suscessfully with hydroxychloroquine and primaquine. There were no central nervous system complications and pulmonary edema. Conclusion : Malaria is one of the important acute febrile illness with the high incidence between July and August.

산화전세정에 따른 표면 Microroughness 및 불량분석에 관한 연구

김경민,유일현,정양희 麗水大學校 産業基術硏究所 1999 産業基術硏究所 論文集 Vol.8 No.-

The purity of wafer surface is an essential requisite for the successful fabrication of VLSI silicon circuits. In the cleaning step, the precleaning(NH₄OH/H₂O₂/H₂O) method has been used. The precleaning process for bare wafer can affect the state of wafer surfaces. It can cause the degeneration of gate oxide layer. For the investigation of this problem, we measured the microroughness of silicon wafer surface using the atomic force microscope(AFM). The microroughness is in the range of 1.1∼1.6Å. The result is increased proportionally to the number of cleaning times. This problem by additional precleaning distributed to edge in the wafer. To avoid th yield drop due to the microroughness, we suggest that the content ratio of precleaning solution is to be at 0.2:1:10 and the cool DI water rinsing should be followed after cleaning step. However, additional cleaning process should be applied carefully to avoid failure even in the optimized precleaning process.

신손상 진단에 있어 현미경적 혈뇨의 의의

임정환,오봉렬,나국주,김현수,윤한덕,허 탁,조석주,민용일 大韓應急醫學會 1996 대한응급의학회지 Vol.7 No.1

The evaluation of patients with microscopic hematuria after blunt abdominal trauma has become controversial. We tested the hypothesis that renal contusion can be diagnosed clinically and that these patients do not require radiographic evaluation. To evaluate the association of microscopic hematuria without shock and with renal injury, we reviewed the medical records of 252 patients following blunt abdominal trauma. Microscopic hematuria without shock was noted in 58 of 252 patients. Of the 58 patients 52 had renal contusion, 4 had renal laceration, 1 had a renal rupture and 1 had renal pedicle injury. All of the patients with renal contusions experienced no complications from nonoperative management. But, avoiding a radiopraphic evaluation in patients with blunt renal trauma plus microscopic hematuria and no shock would miss a few cases of severe renal injury.

중부지역 과수원 토양중의 중금속 함량 평가

정구복,김원일,이종식,신중두,김진호,윤순강 한국환경농학회 2004 한국환경농학회지 Vol.23 No.1

우리나라 중부지역에서 1998년 3∼5월에 과수원 토양 164지점(경기 48, 강원 36, 충북 36, 충남 44지점)을 대상으로 표토(0∼20 ㎝)와 심토(20∼40 ㎝)로 나누어 채취하여 토양내 중금 속함량과 분포특성, 총함량에 대한 침축액별 가용성 함량 비율 및 토양 이화학성과의 관계 를 비교 검토한 결과는 다음과 같다. 과수원 토양중 0.1 N-HCl 침출성 평균함량은 Cd 0.080, Cu 4.23. Pb 3.42 ㎎/㎏, 1 N-HCl 침출성 As 평규함량은 0.44 ㎎/㎏, 중금속 총함량 은 Zn 78.9, Ni 16.09 및 Hg 0.052 ㎎/㎏ 이었다. 과수원 토양내 중금속 평균함량은 우리 나 라 토양환경보전법의 토양오염 우려기준(Cd 1.5, Cu 50, Pb 100, Zn 300, Ni 40, Hg 4 ㎎/ ㎏)과 비교하여 1/25∼1/76.9 수준으로 안전하였다. 토양의 중금속 총함량에 대한 침출액별 가용성 함량비율은 Cd 5.4∼9.2, Cu 27.9∼47.8, Pb 12.6∼21.8, Zn 15.8∼20.3, Ni 5.3∼6.3, Cr 0.7∼3.6% 이었고, 특히 0.05 M-EDTA 침출성 CU 및 Pb의 침출비율이 상대적으로 높 게 나타났다. 토양내 Cd, Pb 및 Ni의 총함량은 모래함량과 부의상관, 미사와 점토함량과는 정의 상관을 보였다. 토양의 중금속 총함량에 대한 침출액별 가용성 함량비율은 점토함량과 는 부의 상관을 보였으며, Zn과 Ni의 함량비율은 토양 pH값, 유기물 및 유효인산 함량과 정 의 상관을 보였다. 이상의 결과에서 볼 때 과수원 토양의 중금속 함량은 토양환경보전법의 토양오염기준보다 매우 낮아 안전하였으나 영농활동에 의한 영향으로 볼 수 있는 농도수준 이 검출된 일부 토양에서 조사되었다. 따라서 최근의 친환경농업 측면으로 볼 때 영농형태 별 중금속의 분포 및 농업자재에 의한 농경지내 중금속 부하량에 근거하여 중금속 오염유무 를 평가할 수 있는 판단기준에 대한 연구가 필요하다고 생각된다. Objectives of this study were to monitor the distribution of heavy metals, to compare extractable heavy metal with total content and to investigate the relationships between soil physico-chemical properties and heavy metals in orchard soil. sampling sites were 48 in Gyeonggi, 36 in Gangwon, 36 in Chungbuk, and 44 in Chungnam. Soils were collected form two depths, 0 to 20 and 20 to 40 cm (here after referred to as upper and lower layers) from March to May in 1998. Total contents of heavy metal in soils were analyzed by ICP-OES after acid digestion (HNO₃:HCl:H₂O₂) whereas extractable contents were measured after successive extraction of 0.1 N-HCl, 0.05 M-EDTA, and 0.005 M-DTPA. Mercury was analysed by mercury atomizer. The average contents of Cd, Cu, and Pb in the extractant with 0.1 N-HCl at upper layer were 0.080, 4.23, and 3.42 ㎎/㎏, respectively. As content in the extractant with 1 N-HCl was 0.44 ㎎/㎏, and total contents of 20, Ni, and Hg were 78.9, 16.1, and 0.052 ㎎/㎏, respectively. m e ratios of concentrations of heavy metals to threshold values (Cd 1.5, Cu 50, Pb 100, Zn 300, Ni 40, Hg 4 ㎎/㎏) in soil Environmental Conservation Act in Korea (2001) were low in the range of 1/2.5-1/76.9 in orchard soils. The ratios of extractable heavy metal to total content ranged 5.4-9.21% for Cd, 27.9-47.8% for Cu, 12.6-21.8% for Pb, 15.8-203% for Zn, 5.3 -6.3% for Nii and 0.7 -3.6% for 2n, respectively. Cu and Pb contents in 0.05 M-EDTA extractable solution were higher than those in the other extractable solution. Total contents of Cd, Ni, and Ni in soils were negatively correlated with sand content, but positively correlated with silt and clay contents. Ratios of extractable heavy metal to total content were negatively correlated with clay content, but Zn and Ni contents were positively correlated with soil pH, organic matter, and available phosphorous. Therefore, the orchard soil was safe because the heavy metal contents of orchard soil were very low as compared to its threshold value in the Soil Environmental Conservation. Act However, it need to consider the input of agricultural materials to the agricultural land for farming practices for assessment of heavy metals.

NOD/SCID 마우스 모델을 이용한 인간 제대혈 혼합이식에서의 생착 양상 분석

정양조,김동욱,조빈,강영주,박보배,김혜정,김태규,오일환 대한조혈모세포이식학회 2002 대한조혈모세포이식학회지 Vol.7 No.2

연구배경: 제대혈은 골수에 비해 많은 장점을 가지고 있음에도, 이식 후 성적에 있어 총세포수에 의해 좌우되며, 한 개의 태반으로부터 얻을 수 있는 총세포수의 한계가 있어 주로 소아에 국한되고 있다. 따라서 제대혈 응용의 확대를 위해 생착량을 늘이기 위한 방법으로 다중공여자에 의한 제대혈 혼합이식을 늘일 수 있는지 연구하기 위하여, 본 연구에서는 주조직적합성 형별로 조합된 제대혈을 NOD/ SCID 생쥐에 이식하는 방법으로, 혼합생착의 가능성을 연구하였다. 방법: 조합된 2개 공여자로부터 얻어진 제대혈들을 단일이식 및 혼합이식하여, 혼합이식된 숙주에 생착된 세포를 PCR-SSOP 방법을 사용하여 공여자 별로 추적하였다. 또한 각 공여자세포의 상대적 생착비율을 대조군인 단일제대혈 이식에서 얻어진 생착량과 비교하는 정량적 비교도 함께 하였다. 결과: 총단핵구를 혼합이식한 실험군에서는 주조직적합성의 일치도에 관계없이 한쪽공여자의 세포가 다른쪽에 비해 우세한 편향생착을 보였다. 그러나 임파구를 비롯한 Lineage 양성세포를 제거한 실험군에서는 주조직적합성의 불일치에도 불구하고 뚜렸한 생착의 공존이 관찰되었다. 결론: 본 연구는 제대혈의 생착량을 증가를 목적으로 총단핵구를 혼합이식할 경우 한쪽공여자의 세포에 의해 편중되며, 이것을 이식 후 생착과정에서의 두 종류의 세포의 경쟁에 의해 초래된다는 것을 보이고 있다. 또한 이러한 경쟁은 제대혈에 포함되어 있던 임파구들에 의한 것이며, 생착공존을 위하여는 공여자간 주조직적합성의 일치도 보다, 임파구의 제거가 더 중요하다는 것을 보여주고 있다. Background: Although umbilical cord blood (UCB) has been an attractive alternatives for bone marrow cells, application is limited to pediatric case due to limitation in total number of nucleated cells, that is a single most important factor for outcomes of UCB transplantation. Therefore, increasing overall engraftment by mixed transplantation of UCB derived from multiple donor should comprise one strategy to circumvent the posed limitation of UCB transplantation. In order to investigate the feasibility of establishing co-engraftment by multi-donor UCB cells, we carried out a xenotransaplantation study using NOD/SCID mice for systemic analysis of results on the input-based control of single unit transplantation. Methods: UCB units with various extent of HLA-matchings were co-transplanted into NOD/SCID mice along with single unit transplantation control. The relative contributions of engraftment by cells from each donor-derived were analyzed by HLA polymorphism using PCR-SSOP. Results: In all HLA-based mixed transplantation of total nucleated UCB cells, engraftment of one donor predominated over the other despite that equivalent amount of engraftments were achieved by single donor transplantation. When lineage depleted UCB units were co-transplanted, significant degree of co-engraftments were observed regardless of HLA disparity. Conclusion: Our result show that one donor cells dominate over the other in mixed UCB transplantation and that it is due to competition between donor cells during post-transplantation process. Our results suggest that immune cells contained in UCB unit mediate such competitioin and that 6 locus HLA matching would not be sufficient to prevent the competition.

活性炭을 利用한 高度淨水處理에 관한 硏究(I)

이성기,김종삼,송영일,김현중 조선대학교 환경공해연구소 2004 環境公害硏究 Vol.18 No.-

The raw water quality is being more polluted recently and other minute organic pollutants are inceasing. so that, good quality water supply is getting more difficult. This study shows organic pollutants removal efficiencies of 2 types pilot plants of Cl process and Bio process in Mongtan water tretment plant. Results in this study can be summarized as follows ; The NH_(3)-N removal efficiency of Cl process was very good, 97% but, that of Bio process was very poor, 10% The THM removal efficiency of GAC process was 95%, and The THMFP removal efficiency of BAC process was 80%. Thus, It is found activated carbon was very effective for the removal of the non-polar organic matter, such as Chloroethylene, Benzene, Toluene, THM et al. The highest THM produced by chlorition are 0.17㎎/ℓ in the winter. therefore, It is found that GAC process is necessary for treatment to THM