Development of a noninvasive KIM-1-based live-imaging technique in the context of a drug-induced kidney-injury mouse model

Tae-Jun Kwon,Da-Sol Lee,Md. Enamul Haque,Rang-Woon Park,Byungheon Lee,Dongkyu Kim,Yong-Hyun Jeon,Kil-Soo Kim,Sang Kyoon Kim 한국실험동물학회 2021 한국실험동물학회 학술발표대회 논문집 Vol.2021 No.7

The development of reliable methods to diagnose acute kidney injury is essential to allow the adoption of early therapeutic interventions and evaluate their effectiveness. Based on the fact that kidney injury molecule-1 (KIM-1) expression levels in kidneys are markedly upregulated early after a damage event, here we developed a noninvasive KIM- 1-based molecular imaging technique to detect kidney injury. First, we took advantage of a phage-display platform to select small peptides demonstrating a specific high binding affinity to KIM-1. The promising candidate was conjugated with fluorescent probes, and its imaging potential was validated in vitro and in vivo. This peptide, with the sequence CNRRRA, not only showed a high imaging potential in vitro, allowing a strong detection of KIM-1 expressing cells by microscopy and flow cytometry but also generated a strong kidney-specific signal in live-imaging in vivo experiments in the context of a drug-induced kidney-injury mouse model. Our data overall suggest that the CNRRRA peptide is a promising probe to use in the context of in vivo imaging for the detection of KIM-1 overexpression in damaged kidneys.

Associations of serotonergic genes with poststroke emotional incontinence

Kim, Jae‐,Min,Stewart, Robert,Kang, Hee‐,Ju,Bae, Kyung‐,Yeol,Kim, Sung‐,Wan,Shin, Il‐,Seon,Kim, Joon‐,Tae,Park, Man‐,Seok,Cho, Ki‐,Hyun,Yoon, Jin‐ John Wiley Sons, Ltd 2012 INTERNATIONAL JOURNAL OF GERIATRIC PSYCHIATRY Vol.27 No.8

<P><B>Objectives</B></P><P>Poststroke emotional incontinence (PSEI) has been associated with serotonergic dysfunction. Polymorphisms of serotonin transporter (5‐HTT) and serotonin 2a receptor (5‐HTR2a) genes may regulate serotonergic signaling at brain synapses, and this study was to investigate associations with PSEI in an East Asian population.</P><P><B>Methods</B></P><P>In 276 stroke cases, PSEI was diagnosed by Kim's criteria. Covariates included age, gender, education, history of depression or stroke, current depression, and stroke severity and location. Genotypes were ascertained for 5‐HTT gene‐linked promoter region (5‐HTTLPR), serotonin transporter intron 2 variable number tandem repeat, 5‐HTR2a 1438A/G, and 5‐HTR2a 102 T/C. Associations with PSEI were estimated by using logistic regression models, and gene–gene interactions were investigated by using the generalized multifactor dimensionality reduction method.</P><P><B>Results</B></P><P>PSEI was present in 37 (13.4%) patients. The 5‐HTT gene‐linked promoter region <I>s</I>/<I>s</I> genotype was independently associated with PSEI. No associations with STin2 VNTR and 5‐HTR2a genes were found, and no significant gene–gene interactions were identified.</P><P><B>Conclusions</B></P><P>Stroke patients with 5‐HTTLPR <I>s</I> allele had higher susceptibility to PSEI, which underlines the potential role of serotonergic pathways in its etiology. Copyright © 2011 John Wiley & Sons, Ltd.</P>

Breeding of Tetraploid in Codonopsis lanceolata (Sieb. et Zucc.) Trautvetter by Colchicine Treatment

Kim,Ik-Hwan,Kim,Hag-Hyun,Hong,Eui-Yon,Yun,Jong-Sun,Yun,Tae,Hwang,Ju-Kwang,Lee,Cheol-Hee 한국자원식물학회 2003 Plant Resources Vol.6 No.3

Present studies were carried out to produce tetraploid plants by colchicine treatment using seeds, seedlings and shoot tips of Codonopsis lanceolata. Three tetraploid plants of C. lanceolata were produced from seeds which absorbed 0.1 % colchicine solution for 12 hours, and 0.5% colchicine solution for 1 and 6 hours from seedlings, respectively. But tetraploid was not produced from shoot tips treated by colchicine solution. Compared to diploid, tetraploid plants had larger stomata, but less number of stomata. Fresh weight of tetraploid plants was 1.4∼3.6 times heavier than diploid plants.

유제놀에 의한 즉시형 알레르기 반응의 억제

김상현,신태용,김형룡,이영미,이은희,신보경,김윤철,안년형,김형민 우석대학교 의약품개발연구소 1996 藥學硏究誌 Vol.1 No.-

The current study evaluates the capacity of eugenol to regulate immediate allergic reaction by control of histamine release. Administrations of eugenol (1 M/kg. i.p.) at 60 min before and 5.10 min after the compound 48/80 treatment (8 mg/kg.i.p.) were shown the mortality rates as 0.44.4. and 77.8%. respectively. A 60 min before administered group revealed a singificant inhibition of serum his-tamine release compared with those of 5 and 10 min after the compound 48/80 injection. Eugenol (6-48mM) was also showed a dose-dependent activity on the compound 48/80-induced histamine release from the highly purified population of Alcian Blue-positive peritoneal mast cells. These results indicate that in vitro treatment with exogenous eugenol inhibited the active response of mast cell populations and modulated its characteristics.

일본 '백화파'에 대한 한일 비교문학적 연구 : 이광수와 김동인을 중심으로 Mainly Lee Kwang-Soo and Kim Dong-In

김종태,강현구 한국현대문예비평학회 2003 한국문예비평연구 Vol.- No.13

After this treatise grasped the point that a character of the Baekhwa school was idealism, egoism, illuminism, and art for art’s sake, it investigated what appearances that these thoughts had reflected on the Korean modern literature to make Lee Kwang-Soo and Kim Dong-In two pivot objects of study. They, Lee Kwang-Soo and Kim Dong-In met with works and thoughts of the Baekhwa school writers to go to Japan about the time when they played an important role in the Japanese literary world. But they who had quite different characters and thoughts accepted the Baekhwa school through a different logical connection with each other. When He, Lee Kwang-Soo had met with works, he formed an enlightenment consciousness of idealism, and Kim Dong-In egoism and art for art’s sake. The Baekhwa school is the representative writers in the Japanese idealism. They are an important writers from the viewpoint of on the comparative literature of Korea & Japan novel because the Baekhwa school had an effect on two writers who formed the basis of the Korean modern novel. We find out that the Baekhwa school had an enormous effect on the Korean literature if we will consider a relative importance Lee Kwang-Soo and Kim Dong-In have come into possession of the history of Korean novel.

스마트폰 블루투스 통신을 사용한 로봇 제어

김영진;김광우;김가덕;윤호성;김태공;서재현 인제대학교 2012 仁濟論叢 Vol.27 No.1

SLIS(Software License Integrated Solution) is composed of the 6 parts, Server Agent, Web Server, Client Agent, Client Manager, Service, and Install Shield. For the most part of the project is developed based on MFC platform except for the one, Client Manager, which is built on DDK(Driver Development Kit) and ASP. NET platform. Client Agent program installed on a customer's computer is implemented in 14 different classes. Its main function is able to limit and uninstall the purchased software by using teminateProcess. The purchased softwares expiration dates on customer's computer is being monitored by both Server agent and Client software itself. Client Manager hides the process of Client Agent program which shouldn't be killed by manipulating Process List in Kernel through DDK. To do this, Client Manager is internally implanted and registered in Windows Service to be ran as a part of windows system.

Thiamine결핍 유도후 쥐 전뇌의 성상교세포 Glial Fibrillary Acidic Protein(GFAP) 면역화학 활성도에 대한 변화

김승현,김희태,김주한,김명호,황세진,김명권,백태경 대한신경과학회 1999 대한신경과학회지 Vol.17 No.1

피질골 절제술을 응용한 견치 및 대구치의 후방 견인

김상철,김선영,김현숙,정혜승,김현태,조진우 대한치과교정학회 2005 대한치과교정학회지 Vol.35 No.2

빠르며 정확하고 안전한 치아이동을 목표로 삼고 있는 교정치료에서 최근 새로이 도입된 피질골 절제술과 견인 골형성술을 응용한 치아이동에 대하여 알아보았다. 특히 견치나 대구치의 후방이동은 기존의 치아 이동 양식으로는 조절이 어렵고, 장기적인 기간에 불가피한 치아이동이다. 피질골 절제술과 견인 골형성술을 동반하여 상당히 효과적인 원심이동을 기할 수 있었던 증례를 통하여 적용 술식, 견인 장치 등을 논하고 그효과를 파악하였다. 이런 술식을 통해 빠른 치아 이동과 이에 따른 전반적인 치료기간의 감소가 가능하였으며, 무리한 치아이동에서 발생할 수 있는 고정원 소실이나 치근흡수, 치주조직의 파괴 같은 부작용도 줄일 수 있었다. Tooth movement facilitated by corticotomy and distraction osteogenesis was discussed. In this study, a portion of cortical bone which can provide resistance to tooth movement in alveolar bone was removed. Active bone deposition was then possible in the tension side. Teeth moved at such a speedy rate as we could not imagine from conventional orthodontic treatment, which lead to the reduction of the total treatment period. Posterior movement of the canine or molar teeth was possible without any side effects such as anchorage loss, root resorption or periodontal breakdown.

비혈연간 동종골수이식에 의해 치료된 중증 재생불량성빈혈 1예

김현중,이홍기,이방훈,이창근,이종태,정현식,김원석,윤성수,강원기,박근칠,김대식,고영혜,박찬형 대한조혈모세포이식학회 1997 대한조혈모세포이식학회지 Vol.2 No.1

비혈연간 동종골수이식은 혈연간 동종골수이식이 불가능한 환자에서 기존의 면역역제요법으로 효과가 없었던 경우나 재발한 경우에 적응증이 될수 있다. 이식 거부와 이식편대숙주병의 발생이 골수 이식의 성공률을 감소시키므로, 보다 강력한 conditioning regimen의 사용과 T cell이 제거된 골수를 사용하는 것이 필요하다. 기존의 high dose corticosteroid나 cyclosporine에 대하여 불응성인 이식편대숙주병의 치료를 위해서 ATG와 Mycophenolate mofetil (Cellcept®) 의 사용이나 Tacrolimus, Methotrexate와 같은 다른 면역억제제치료를 고려해 볼 수 있다. It is known that allogenic bone marrow transplantation is the most effective treatment for aplastic anemia. However, this treatment is only applicable to a minority of patients because the proportion of patients who have HLA-matched siblings about 25% to 30% of all the aplastic anemia patients. In the case of the absence of HLA-matched donor, unrelated allogenic bone marrow transplantation is considered. However, (because of severe acute and chronic GVHD and graft failure) the success rate of this approach is only 20~30%. Recently, it was reported that increased immunosuppressive condition combined with T cell depletion of the marrow graft would result in greater success. There was no case of successfully treated aplastic anemia patient by unrelated allogenic bone marrow transplantation in Korea. Here, we report a case of severe aplastic anemia successfully treated with unrelated allogenic bone marrow transplantation.

쇄석분진을 이용한 발포 건축재의 제조

김태훈,김기현,김현태 영남대학교 공업기술연구소 2000 工業技術硏究所論文集 Vol.28 No.1

A rock dust as industrial waste during rock crushing was recycled to make a porous ceramics. Raw materials were mixed as a portion of rock dust(70vo1%), saw dust(30vo1%), bloating agents(10wt% of rock dust). The plate and tube shaped specimens were formed under the pressure of 1ton/㎠. The specimens were bloated at 1150℃ for 1 hour in the atmospheric ambient. The bloated porous ceramics have the characteristics-bulk density 0.6∼0.8g/㎤, compressive strength 77∼200kg/㎠ and thermal conductivity 0.58W/mK. Above the characteristics show that the bloated porous ceramics can be used in the field of refractory materials and light-weight constructional materials.