Identification of a sensitive urinary biomarker, selenium-binding protein 1, for early detection of acute kidney injury

Kim, Kyeong Seok,Yang, Hun Yong,Song, Hosup,Kang, Ye Rim,Kwon, JiHoon,An, JiHye,Son, Ji Yeon,Kwack, Seung Jun,Kim, Young-Mi,Bae, Ok-Nam,Ahn, Mee-Young,Lee, Jaewon,Yoon, Sungpil,Lee, Byung μ,Kim, Hyung TAYLOR & FRANCIS 2017 Journal of Toxicology and Environmental Health Vol.80 No.9

<P>Acute kidney injury (AKI) is associated with increased mortality rate in patients but clinically available biomarkers for disease detection are currently not available. Recently, a new biomarker, selenium-binding protein 1 (SBP1), was identified for detection of nephrotoxicity using proteomic analysis. The aim of this study was to assess the sensitivity of urinary SBP1 levels as an early detection of AKI using animal models such as cisplatin or ischemia/reperfusion (I/R). Sprague-Dawley rats were injected with cisplatin (6 mg/kg, once i.p.) and sacrificed at 1, 3, or 5 days after treatment. Ischemia was achieved by bilaterally occluding both kidneys with a microvascular clamp for 45 min and verified visually by a change in tissue color. After post-reperfusion, urine samples were collected at 9, 24, and 48 hr intervals. Urinary excretion of protein-based biomarkers was measured by Western blot analysis. In cisplatin-treated rats, mild histopathologic alterations were noted at day 1 which became severe at day 3. Blood urea nitrogen (BUN) and serum creatinine (SCr) levels were significantly increased at day 3. Levels of urinary excretion of SBP1, neutrophil gelatinase-associated lipocalin (NGAL), and a tissue inhibitor of metalloproteinase-1 (TIMP-1) were markedly elevated at day 3 and 5 following drug treatment. In the vehicle-treated I/R group, serum levels of BUN and SCr and AST activity were significantly increased compared to sham. Urinary excretion of SBP1 and NGAL rose markedly following I/R. The urinary levels of SBP1, NGAL, TIMP-1, and KIM-1 proteins excreted by AKI patients and normal subjects were compared. Among these proteins, a marked rise in SBP1 was observed in urine of patients with AKI compared to normal subjects. Based upon receiver-operator curves (ROC), SBP1 displayed a higher area under the curve (AUC) scores than levels of SCr, BUN, total protein, and glucose. In particular, SBP1 protein was readily detected in small amounts of urine without purification. Data thus indicate that urinary excretion of SBP1 may be useful as a reliable biomarker for early diagnosis of AKI in patients.</P>

비장애 아동과 전반적 발달장애 아동에서 회전 후 안구진탕 기간의 비교

김진미,송지원,홍은경,김성희,김경미 대한감각통합치료학회 2005 대한감각통합치료학회지 Vol.3 No.1

Objective : The purpose of this study was to get the mean of duration of post-rotatory nystagmus test in normal children and to differentiate the duration of post-rotatory nystagmus test between normal children and children with pervasive developmental disorder. Method : 84 subjects were between 3 and 5 years of age and consisted of 64 normal children and 20 children with the pervasive developmental disorder. Analysis of the data was done by using t-test and ANOVA. Results : The results were as follows: 1. Range of duration of post-rotatory nyatagmus test in normal children was 5-22second on left and 7-21 second on right and the mean was 12.63 second on left and 12.59 second on right. 2. Range of duration of post-rotatory nystagmus test in children with the pervasive developmental disorder was 3-11 second on both and the mean was 5.65 second on left and 5.60 second on right. 3. There was no significant difference between males and females with normal children in duration of post-rotatory nystagmus test. However, there was a significant difference of the mean duration between 3 and 5 years old normal group. 4. Children with pervasive developmental disorder significantly have relatively lower duration than the duration of post-rotatory nystagmus test of normal children. Conclusions : The results of the study showed significant difference between normal children and children with pervasive developmental disorder in duration of post-rotatory nystagmus test and suggest that they could be applied to the baseline of clinical therapy.

위절제술 환자의 표준진료지침 개발 및 적용 효과

김은희,김철규,이순교,김순덕,이혜옥,권정순,이경미,이민미,심순미,유용만,신종식,강은희,이상일,김병식,오성태,육정환,박수길 한국의료QA학회 2003 한국의료질향상학회지 Vol.10 No.2

Background : Gastric cancer is the most common malignant tumor in Korea. surgical operation is one of the major treatment modalities for gastric cancer patients. Therefore, gastrectomy is one of the most common procedures in General Surgery. There were variation in length of hospital stay and medical treatment for gastrectomy between three surgeons at Asan Medical Center. Clinical pathways have received considerable attention as a tool for recucing the medical practice variation, increasing the efficiency of care process, and improving the quality of care. The aim of this study was to evaluate the effect of a clinical pathway for gastrectomy in gastric cancer patients. Methods : The clinical pathway for gastrectomy was developed and implemented by a multidisciplinary group in Asan Medical Center. A computerized clinical pathway program was developed and revised after a pilot test. A total of 145 patients underwent gastrectomy by three surgeons at Asan Medical Center. We compared the length of hospital stay, patient satisfaction, and unplanned readmission rate between the pre-pathway group(n=67) and the post-pathway group(n=78). We also investigated the degree of satisfaction among the physicians and nurses who were main end-users of the clinical pathway. Results : The clinical pathway was applied to all target patients. The average length of hospital stay was shortened from 12.7days to 10.6days(p<0.01). The degree of patient satisfaction with the care process changed from 90.3% to 89.2% after the implementation of the clinical pathway, but the difference was of satistically significant(p=0.761). Unplanned readmission rate was 2.9% in the pre-pathway group. More than 90% of physicians and nurses answered that the clinical pathway had been a useful tool in their medical practice. Conclusions : The findings of the study demonstrated that implementation of the clinical pathway for gastrectomy produced substantial reduction in the length of hospital stay while improving the quality of patient outcomes. The computerized clinical pathway program can be used as one of the powerful patient management tools for reducing the practice variations and increasing the efficiency of care process in Korea hospital settings.

관상동맥 스텐트 삽입술을 시행한 환자에서 안지오텐신 전환효소 억제제와 안지오텐신 수용체 차단제가 재협착에 미치는 영향

김연경,정진원,윤경호,박은미,송미진,최준호,이은미,유남진,김남호,오석규 圓光大學校 醫科學硏究所 2006 圓光醫科學 Vol.21 No.1

민들레 잎과 뿌리 분말을 첨가한 설기떡의 품질 특성

유경미,김세희,장정화,황인경,김경임,김성수,김영찬 한국조리과학회 2005 한국식품조리과학회지 Vol.21 No.1

In order to develop a dandelion (Taraxacum officinale) with natural food applications, the effects of dandelion leaves and roots powder content on the physical, textural and sensory properties of sulggidduk were examined. Dandelion powders of leaves and roots at 0, 1, 2 and 3% were added to the dandelion sulgidduk. In the dandelion roots powder in sulgidduk, the color intensity of L values and the values and moisture content decreased however, the sensory color intensity, dandelion flavor and overall acceptability increased with increasing the dandelion powder content. The sensory overall acceptability indicated the dandelion roots powder content at 3% had the highest overall and flavor scores. As the dandelion leaves content was increased, the moisture contents, lightness, adhesiveness and cohesiveness of sulggidduk decreased, while the sensory color intensity, sensory hardness, hardness of texture properties and dandelion flavor increased. The addition at 1% of the dandelion leaves powder content with the dandelion sulggidduk and the addition at 3% of the dandelion roots powder content with the dandelion sulggidduk showed the highest overall acceptability.

급성 관동맥증후군 환자의 신기능 이상과 주요 심장사건과의 관계

이상재,윤경호,최준호,송미진,김연경,박은미,김현정,이은미,유남진,김남호,오석규,정진원 圓光大學校 醫科學硏究所 2006 圓光醫科學 Vol.21 No.1

效率的인 都市成長管理를 위한 開發制限區域 接境地域의 管理方案

金瑛,趙宰英,文美景 경상대학교 생산기술연구소 2000 生産技術硏究所論文集 Vol.16 No.-

For classification of the border areas, this study attempts a discriminant analysis using two kinds of main characteristics: land-use variables and land-price variables; consequen1ty the management strategy of the border areas is subdivided into 4 functional types according to hit ratio. The results of this study can be summarized as follows. (1) A-type area requires management strategy connected to town's function because it is similar to high-density-town. (2) B-type area resulted from not only a restrictive land-use control but also in need of environmental preservation under development pressure. Accordingly the management of this type of area requires especially residential participation. (3) Since C-type area is in close connection to the suburbs of towns, the management of this area demands both conservation and development through environmentally affection and sustainable management strategy. (4) The management of D-type area is closely related to that of Green Belt area because it is given more developmental pressure with town expanding in consideration of land-use and land-price. And since this area is used for woodland or farmland, it is necessary to prevent from sprawling development and conserve environment.

미혼 남녀의 배우자 선택 조건 및 관련 요인 분석

김경신,김오남,이선미 全南大學校家政科學硏究所 1995 生活科學硏究 Vol.5 No.-

The purposes of this article are to find the general trends of unmarried men and women's mate selection conditions, to estimate the differences according to related variables and to analyze the effects of significant variables influencing on mate selection. The samples were selected from the unmarried 164 men and 235 women living in Kwang-ju. The major findings are as follows; 1. All scores of mate selection conditions were higher than medium. Especially in desirable spouse conditions, women's were higher than men's. Move valued spouse conditions were women's apperance, health, family background in men and all conditions in women. Women desired more similar and interactional conditions than men. 2. External and internal conditions were significantly different from age, work in men and age, father's and mother's age in women. Homogeneous and interactional conditions were significantly different from sex role attitude, premarital sexual experience in men and age, education, work, father's age, love necessity of marriage, sex role attitude, premarital sexual experience in women. 3. External and internal conditions were influenced by work in men and mother's and father's age in women. Homogeneous conditions were influenced by premarital sexual experience, necessity of marriage in men and age, premarital sexual experi-ence, necessity of marriage in women. Interactional conditions were influenced by sex role attitude, father's age in men and sex role attitude, education, work in women.

지적장애 아동의 균형과 호흡 기능 향상을 위한 감각통합치료: 개별 실험 연구

김경미,홍은경 대한감각통합치료학회 2011 대한감각통합치료학회지 Vol.9 No.1

Objective : Sensory integration is the organization for sensation for use. The purpose of this study is to carry out the sensory integrative intervention for the mental retardation with child and to examine change of child's balance and breath abilities. Methods : The study performed from 25 May to 13 Autumn, 2009. The design is AB design(baseline 3sessions, intervention 21sessions) of single -subject research design. We carried out the sensory integrative intervention(3 sessions per week) for baseline period and the sensory integrative intervention(3 sessions per week) with sensory diet every day for intervention period. Results : The results of the sensory integrative intervention with sensory diet had positive effects for child's breathe and balance. Conclusion : The occupational therapists recognize the effects of the sensory integrative intervention with sensory diet and need to recommend for the family of child with disability. 목적 : 감각통합치료는 개별아동에 맞는 감각의 기회를 제공하고, 적절한 도전의 과제를 제공하여 적응반응을 이끌 어 내는 아동 중심의 접근법이다. 본 연구는 지적장애 아동에게 적합한 감각통합치료를 적용하여 아동의 균형과 호흡능력의 변화를 살펴보고자 하였다. 연구방법 : 연구 대상은 만 5세 3개월의 남아로 지적장애 진단을 받았다. 실험기간은 2009년 5월 25일부터 2009 년 8월 13일까지이었다. 실험설계는 개별실험연구(single-subject research design) 중 AB 설계를 사용하였고, 기초선 A(감각통합치료) 3회기와 중재 B(감각통합치료, 감각식이) 21회기이었다. 실험기간 동안 아동의 문제점 인 균형과 호흡의 변화를 측정하였다. 결과 : 첫째, 감각통합치료는 아동의 호흡능력에 긍정적인 영향을 미쳤다. 둘째, 감각통합치료는 아동의 균형에 긍 정적인 영향을 주었다. 결론 : 작업치료사는 지적장애아동의 균형과 호흡능력을 향상시키기 위해 감각통합치료를 지속적으로 적용, 확대 하는 것이 필요하다.

석청(Mad-honey) 복용 후 유발된 부정맥 1예

김용철,김남호,김승환,최준호,박은미,이상재,이은미,유남진,윤경호,오석규,정진원 圓光大學校 醫科學硏究所 2008 圓光醫科學 Vol.23 No.2

석청에 포함된 grayanotoxin에 의해 독성 작용이 나올 수 있으며, 이러한 독성 작용은 일반적으로 24시간 이내 저절로 회복된다. 최근에 본 저자들은 석청 복용 후 발생한 심실빈맥을 경험하였기에 보고하는 바이다. 49세 남자가 호흡곤란으로 내원하였으며, 내원당시 분당 40회 정도의 동성 서맥이 관찰되었고, 수액을 투여하던 중 심실 빈맥이 발생하였다. 항부정맥제를 투여 후 정상 동율동으로 전환되었으며, 특별한 이상 없이 4일 후 퇴원하였다. Mad-honey intoxication caused by the consumption of honey producted from the nectar of rhododendrons. The grayanotoxins cause the intoxication. The toxic effects of mad-honey poisoning are rarely fatal and generally last for no more than 24 hours. We experienced one case, a 49 years-old man who presented with dyspnea after ingestion of mad-honey. He showed marked sinus bradycardia with < 40 beats per minute on admission. The cardiac rhythm was changed to ventricular tachycardia immediately. These features resolved completely in 24 hours with continuous infusion of amiodarone(600 mg per day) and fluids. We report the case of intoxication of mad-honey as a presentation of fatal cardiac arrhythmia.