Systemic administration of low dosage of tetanus toxin decreases cell proliferation and neuroblast differentiation in the mouse hippocampal dentate gyrus

Bing Chun Yan,In Hye Kim,Joon Ha Park,Ji Hyeon Ahn,Jeong-Hwi Cho,Bai Hui Chen,Jae-Chul Lee,Jung Hoon Choi,Ki-Yeon Yoo,Choong Hyun Lee,Jun Hwi Cho,Jong-Dai Kim,Moo-Ho Won 한국실험동물학회 2013 Laboratory Animal Research Vol.29 No.3

In the present study, we investigated the effect of Tetaus toxin (TeT) on cell proliferation and neuroblast differentiation using specific markers: 5-bromo-2-deoxyuridine (BrdU) as an exogenous marker for cell proliferation, Ki-67 as an endogenous marker for cell proliferation and doublecortin (DCX) as a marker for neuroblasts in the mouse hippocampal dentate gyrus (DG) after TeT treatment. Mice were intraperitoneally administered 2.5 and 10 ng/kg TeT and sacrificed 15 days after the treatment. In both the TeT-treated groups, no neuronal death occurred in any layers of the DG using neuronal nuclei (NeuN, a neuron nuclei maker) and Fluoro-Jade B (F-J B, a high-affinity fluorescent marker for the localization of neuronal degeneration). In addition, no significant change in glial activation in both the 2.5 and 10 ng/kg TeT-treated-groups was found by GFAP (a marker for astrocytes) and Iba-1 (a marker for microglia) immunohistochemistry. However, in the 2.5 ng/kg TeT-treated-group, the mean number of BrdU, Ki-67 and DCX immunoreactive cells, respectively, were apparently decreased compared to the control group, and the mean number of each in the 10 ng/kg TeT-treated-group was much more decreased. In addition, processes of DCX-immunoreactive cells, which projected into the molecular layer, were short compared to those in the control group. In brief, our present results show that low dosage (10 ng/kg) TeT treatment apparently decreased cell proliferation and neuroblast differentiation in the mouse hippocampal DG without distinct gliosis as well as any loss of adult neurons.

흰쥐에서 할로페리돌에 의해서 유발된 강경증에 미치는 후구 적출의 영향

전승호,김선장,배기환,이순철 충남대학교 약학대학 의약품개발연구소 1992 藥學論文集 Vol.8 No.-

We attempted to clarify the effect of bilateral olfactory bulbectomy on catalepsy induced by haloperidol in rats. The incidence of catalepsy induced by haloperidol remarkably increased after lesion of olfactory bulb, which was significantly inhibited by L-5-hydroxytryptophan, L-DOPA, and ginseng's total saponin but reserpine and α-methyl-p-tyrosine were ineffective. The dopamine content of brain was significantly decreased by olfactory bulbectomy, but this result was reversed by ginseng's total saponin.

모형식품의 리올로지 특성

천기철,박영덕,장규섭 충남대학교 농업과학연구소 1995 농업과학연구 Vol.22 No.1

The model foods were prepared by simulating mositure, protein and starch, and they were heated for 30 mins, at 80℃ and then cooled at 25℃ in water bath. Their rheological properties were investigated by the use of Brookfield wide-gap rotational viscometer at 30~60℃, and the rotation speed ranged from 0.6 to 6 rpm and solid content ranged from 8% to 11%, the results obtained were as follows. 1. All the model foods (P_1S_3, P_2S_2, P_1S_1, P_2S_1, P_3S_1, P_4S_0) exhibited pseudoplastic behaviors with yeild stress and were thixotropic foods which showed time - dependent structural decays, but the starch food of 8~11 % solid content did not show the flow behavior. 2. The correlation between the rheological parameters and the protein content of model foods in various moisture content did not appeared a constant relationship. 3. The change of shear stress against shear rate in high starch foods was larger than that in high protein foods and the structure at initial shear time was decayed with a quatic equation according to the Tiu's Model and structural decay was in parallel with the increase of shear rate. 4. The temperature dependency of the apparent viscosity of P_1S_2, and P_2S_1 was fully expressed by Arrhenius equation and activation energies of their food were 2.35 and 1.34 Kcal/g·mol, respectively.

『職方外紀』의 저술 의도와 조선 지식인들의 반응

천기철 부산경남사학회 2003 역사와 경계 Vol.47 No.-

Jikbangoegi(職方外紀), an encyclopedic writing including geography, cosmology, manners and so on, of the world, was written by a Jesuit father, Julio Aleni(艾儒略, 1582-1649). The climate and people's conditions of nations which were not within the jurisdiction of Chinese Jikbangsa(職方司) are recorded in this book. It provided a detailed information on the outside world beyond China to Choseon. It had a great influence on intellectuals which took an interest in scientific knowledge at that time and helped to change their world view. It was important in studies of scientific history for it had a great effect on astronomy and geography. Nevertheless, Jikbangoegi have not been much studied by Koreanl scholars. Because it was written in a very difficult Chinese writing, it is hard to understand this book for students. I have translated it for two years and a half. This paper is to finish my translation. I examined the tendency of study in Jikbangoegi, Aleni's growth circumstances, his intention to write it, its contents and its effect on the Confucian scholars in Choseon. In Chapter 2, I tried to understand Aleni's hardship by investigating his journey from Europe to China. I also pursued the process of writing of Jikbangoegi. In Chapter 3, I analyzed its contents and intentions. In Chapter 4, I considered intellectuals' responses in Choseon to Jikbangoe culture(職方外 文化). My conclusion is that Jikgnaoegi had a great effect on the change of intellectual's world view in the later Choseon period.

작은 피질하 병변에 대한 정위적 개두술 및 경-구 접근법

손병철,김문찬,이관성,박춘근,홍용길,강준기 대한신경외과학회 1999 Journal of Korean neurosurgical society Vol.28 No.12

급성충소돌기염의 임상 : 병리학적 고찰

양기화,조준필,하영천,한진우,정학재,손수창,이성희,황재훈,신철호 최신의학사 1989 最新醫學 Vol.32 No.4

Benz(a)anthracene이 마우스태자 간조직 세포의 소핵출현에 미치는 경태반효과

成耆天,張聖薰,車喆煥 고려대학교 의과대학 1989 고려대 의대 잡지 Vol.26 No.1

In order to adduce the cytogenic effect of Benz(a)anthracene(B(a)A) in pregnant mice through placental barrier to fetus, 125㎎/㎏, 250㎎/㎏, 500㎎/㎏, of B(a)A were administrated to groups of mice 16th pregnancy day About 20 hrs later, freauency of micronuclei in polychromatophilic normoblasts of liver tissues extracted from fetus was measured At the same time, 200 ㎎/㎏ and 400 ㎎/㎏ of Benzo(a) pyrene(B(a)P) were administered with same procedure to compare the frequencies between in cases of B(a)A and B(a)P administration. Especially incase of B(a)A administration, frequencies of their appearances in bone marrows of mother mice and livers of fetuses were compared. On the other hand, pathological changes of fetus liver caused by B(a)A and B(a)P in mothers were observed through microscopic examination in order to reconfirm and compare their transplacental effects. As the results, cytogenic toxicity for feture caused of B(a)A was confirmed. It means that some amount of B(a)A which was administered to pregnant mice could be transmitted to fetus through placental barriers Mutagenicity caused of B(a)A was higher in fetus than mother mouse. The cytogenic toxicity caused of B(a)A both for mother mouse and fetus was lower than that of B(a)p.

소아 동종 조혈모세포이식 200예의 분석 : Single Center Study

김학기,조빈,정낙균,정대철,장필상,김선영,김춘추 대한조혈모세포이식학회 2002 대한조혈모세포이식학회지 Vol.7 No.1

연구배경: 1983년 11월부터 2000년 9월까지 시행한 동종 조혈모세포이식의 성적을 분석하여 소아 골수이식의 발전 방향을 모색하고자 하였다. 방법: 1983년 11월부터 2000년 9월까지 가톨릭의대 조혈모세포이식센터, 성모병원 소아과에서 동종 조혈모세포이식을 시행한 200예를 대상으로 조혈모세포이식의 유형 및 대상질환, 이식 당시의 상태 및 전처치에 따른 치료 성적을 후향적으로 분석하였다. 결과: 200예 중 HLA-일치 형제간 골수이식이 146예(73%), HLA-일치 형제간 골수이식 이외의 이식이 54예(27%)였다. 1) HLA-일치 형제간 골수이식: 146예 중 남아와 여아가 각각 78명과 68명이었으며 SAA 50예의 5년 무병 생존율은 95.8±2.9%였고 AML 45예의 5년 무병 생존율은 71.7±7.0%로 일차 및 이차 관해기에 이식 받은 40예와 5예의 5년 무병 생존율은 각각 73.6±7.2%와 60.0±21.9%였다. AML에서 전처치로 Bu/Cy를 사용한 28예와 TBI/Cy를 사용하였던 9예의 5년 무병 생존율은 각각 81.9±7.4%와 33.3±15.7%였으며 Bu/Cu/TBI를 시행한 8예의 무병 생존율은 80.0±17.9%였다. ALL 33예의 5년 이상 무병 생존율은 75.5±7.5%였고 일차 및 이차 관해기에 이식을 시행하였던 18예와 14예의 무병 생존율은 각각 83.0±9.0%와 71.4±12.1%였다. CML 및 MDS 11예의 5년 무병 생존율은 72.7±13.4%였다. 기타 질환으로는 Fanconi 빈혈 2예, 혈구탐식성 조직구증식증 2예, 순적혈구 빈혈 2예, Kostmann 증후군이 1예로 이들의 전체 무병 생존율은 85.7±13.2%였다. 2) HLA-일치 형제간 골수이식 이외의 이식을 시행하였던 54예의 3년 이상 무병 생존율은 45.3±11.6%였으며 HLA-일치 UBMT 26예, CBSCT 15예, 형제 이외의 혈연에 의한 골수이식이 13예로 이들의 무병 생존율은 각각 56.2±10.8%, 58.2±13.1%, 35.9±17.3%였다. 결론: 소아 난치성 혈액질환의 완치요법으로 동종 골수이식의 치료효과를 확인할 수 있었으며, 동종 조혈모세포의 공여원으로 비혈연간 골수나 제대혈의 이용이 점차 증가하고 있음을 알 수 있었다. Background: Bone marrow transplantation was first introduced to Korean children in 1983. Since then the number of children receiving transplants has increased steadily. Methods: We analyzed two hundred pediatric cases of allogeneic hematopoietic stem cell transplantation between Nov. 1983 and Sep. 2000 in Catholic Hematopoietic Stem Cell Transplantation Center of Korea. Results: HLA-matched sibling transplantations were performed in 146 cases (78 males, 68 females, median age; 10 years) with median follow-up of 47 months. The 3-year estimated event-free survival (EFS) of ALL and AML was 76% and 72% respectively. The 5-year estimated EFS of severe aplastic anemia and CML/MDS was 96% and 73% respectively. The 2-year estimated EFS of nonmalignant rare disease was 86%. Twenty-six children underwent unrelated bone marrow transplantation (UBMT), 15 cord blood stem cell transplanta-tion (CBSCT) and 13 familial haploidentical transplantation (FHT). There were 35 males and 19 females with a median age of 6.5 years and median follow-up of 18.5 months. The estimated 2-year EFS of UBMT, CBSCT and FHT were 56%, 58% and 34% respectively. Conclusion: HLA-matched sibling allogeneic BMT showed better survival in children with hematopoietic stem cell disorder compared to UBMT and CBSCT. Recently, transplants using alternative stem cell source are increasing due to lack of suitable matched sibling donor and continuous efforts for reducing transplant-related complications are warranted for improving survival.

내측회장동맥에 의해 형성된 척수경막동정맥루로 인한 척수병증 1예

이일형,배재천,이상무,김진혁,권기한,이병철 대한신경과학회 1999 대한신경과학회지 Vol.17 No.3

농촌지역 노인들의 우울증상에 관련된 요인들의 특성

송미숙,전기홍,송현종,김정은,박승구,신상건,이동훈,정성현,최연주,최완철,최민규,황재철,홍지만 아주대학교 1997 아주의학 Vol.2 No.2

This study was performed to identify risk factors for depression among the elderly in a rural area. A survey was done for the elderly living in Gosam-myenn, Ansung-kun during the period of July 28, 1997 and July 30, 1997. Demographic and sodoeconornic characteristics, and functional and health Status were assessed using a structured questionnaire, and the level of depression was measured by GDS score. The data obtained were analyzed by frequencies, percentage, t-test, ANOVA, and multiple regression, and the results were as follows: 1. The total sample consisted of 133 elderly. Of them, 47 elderly (35.4%) showed significant depressive Symptoms. 2. The GDS score was the highest for the group over 85 years of age (p < 0.05). Those living alone or widowed showed lower GDS score than those living with someone or married (p < 0.05). 3. The GDS score was the highest in the absolutely dependent group, followed by the partially dependent group, and the independent group (p < 0.01). The elderly who perceived their health Status as excellent showed the lowest GDS score. In contrast, those who had more than five chronic diseases showed the highest GDS score. 4. The elderly receiving financial and emotional support from their family or friends showed lower GDS score compared to those without any support. 5. The economically dependent group showed higher GDS score than that of independent group. 6. The elderly who involved in leisure activities showed lower GDS score than those without any involvement. 7. In terms of risk factors for depression, we found that perceived health Status, subjective economic Status, leisure activities, living arrangement and financial support were inversely associated with GDS score, whereas labor support was positively associated with GDS score. Based upon the above findings, this study suggests that these risk factors for depression in the elderly should be taken into consideration for a comprehensive mental health program for the elderly living in the rural area.