Comparison of Proliferative Activity in Each Histological Subtypes of Benign and Atypical Intracranial Meningiomas by PCNA and Ki-67 Immunolabeling

최승진,장은덕,권성오,계대곤,박춘근,이상원,강준기,Choi, Seung Jin,Chang, Eun Deok,Kwon, Seung Oh,Kye, Dae Kon,Park, Choon Keun,Lee, Sang Won,Kang, Joon Ki The Korean Neurosurgical Society 2000 Journal of Korean neurosurgical society Vol.29 No.9

목 적 : 양성 뇌수막종에 비하여 이형성 및 악성 뇌수막종이 나쁜 임상적인 예후 및 양상을 보이는 것은 잘 알려져 있으나, 양성 뇌수막종에 있어서 각각의 병리조직학적 아형에 따른 생물학적 양상의 차이에 대해서는 잘 알려지지 않거나 일부 논란이 되고있다. 본 연구에서는 이형성 뇌수막종 및 양성 뇌수막종의 각각의 병리조직학적 아형에 따른 증식능의 차이여부를 알아보고자 PCNA와 Ki-67표지지수를 분석하였다. 방 법 : 본원에서 뇌수막종으로 수술을 시행하여 얻은, 재발을 보여 재수술을 시행한 2례를 포함하여, 파라핀에 포매시킨 27개의 조직을 대상으로 병리학적인 증식능을 분석하기 위해, PCNA에 대한 단일항체 및 MIB-1 단일항체를 이용한 면역조직화학적 염색을 시행하였다. 조직학적 분류상 meningothelial type이 8례, transitional type이 9례, fibroblastic type이 5례였으며, 이형성 수막종이 5례였다. 결 과 : PCNA표지지수의 평균값은 양성 수막종에서 meningothelial type이 $4.82{\pm}5.10%$, transitional type이 $9.01{\pm}4.25%$, fibroblastic type이 $5.66{\pm}5.32%$를 보였으나 이형성 수막종에서는 $27.62{\pm}19.67%$의 높은 지수를 나타냈고, Ki-67 표지지수의 평균값은 양성 수막종의 아형에서 각각 $0.43{\pm}0.85%$, $0.44{\pm}1.08%$, $0.24{\pm}0.18%$를 보이고, 역시 이형성 수막종에서는 $0.84{\pm}0.59%$의 높은 지수를 보였다. 즉, 양성 수막종에서 각각의 아형에 따른 PCNA 및 Ki-67 표지지수는 통계학적으로 의미있는 차이는 없었으나(p>0.05), 이형성 수막종에서는 의미있는 높은 표지지수를 보여(p<0.05) 양성 수막종에서 보다 높은 증식능을 보임을 알 수 있었다. 결 론 : PCNA 및 Ki-67 표지지수를 이용한 증식능의 비교결과, 양성 뇌수막종에서는 각각의 아형에 따른 생물학적 양상이나 예후는 차이가 없을것으로 생각되나, 이형성 수막종에서는 높은 증식능을 보여 이에 대한 예후를 예상할 수 있을것으로 생각되며, 또한 이러한 표지지수가 병리조직학적으로 양성과 이형성의 감별에 많은 도움이 될것으로 사료된다. Objective : The clinical prognosis and biological behavior of atypical and especially malignant meningiomas are well known to be worse than benign meningioma, but the degree of biological aggressiveness in each classical subtypes of benign meningioma is controversy. This study was performed to see whether there is a difference in the proliferative activity between each different histological subtypes of benign meningioma as well as atypical meningioma. Methods : Paraffin-embedded surgical specimens of 27 meningiomas, including two recurrent tumors, were studied to evaluate proliferative activity by immunohistochemical method with monoclonal antibodies to proliferating cell nuclear antigen(PCNA) and MIB-1. The specimens consisted of 8 cases of meningothelial, 9 cases of transitional, 5 cases of fibroblastic subtypes and 5 cases of atypical meningiomas. Results : Mean PCNA labeling indices of meningothelial, transitional and fibroblastic meningiomas were $4.82{\pm}5.10%$, $9.01{\pm}4.25%$ and $5.66{\pm}5.32%$, but that of atypical meningiomas was $27.62{\pm}19.67%$, noting a higher value compared to all three subtypes of benign meningiomas. Mean Ki-67 labeling indices of the above 3 subtypes were $0.43{\pm}0.85%$, $0.44{\pm}1.08%$ and $0.24{\pm}0.18%$, and that of atypical meningiomas was also revealed to be of higher value ($0.84{\pm}0.59%$). PCNA and Ki-67 labeling indices were not statistically different between histological subtypes of benign meningioma(p>0.05), but the differences of both immunolabeling between benign and atypical meningiomas were statistically significant(p<0.05). Conclusion : Immunolabeling of PCNA and Ki-67 in intracranial meningiomas reveals no prognostic difference between meningothelial, transitional and fibroblastic subtypes in classical benign meningiomas by measuring expression of PCNA and Ki-67, but it seems to be helpful in differentiating benign and atypical meningioma, later showing more proliferative activity and biological aggressiveness.

Effects of <i>Ginkgo biloba</i> Extract on Promotion of Neurogenesis in the Hippocampal Dentate Gyrus in C57BL/6 Mice

YOO, Dae Young,NAM, YoonYi,KIM, Woosuk,YOO, Ki-Yeon,PARK, Jaeil,LEE, Choong Hyun,CHOI, Jung Hoon,YOON, Yeo Sung,KIM, Dong-Woo,WON, Moo-Ho,HWANG, In Koo Japanese Society of Veterinary Science 2011 The Journal of veterinary medical science Vol.73 No.1

<P><I>Ginkgo biloba </I>leaf extract (Gb) has been known to improve blood flow and preclude the tissue from free radical damage. Effects of Gb were examined by using Ki67, a specific proliferative marker for cellular proliferation, and doublecortin (DCX), a marker for immature neurons, indicating degree of neuroblast differentiation in the hippocampal dentate gyrus (DG) of adult C57BL/6 mice. The mice were fed with Gb at 40 and 100 mg/kg once daily for 28 days. The increase of Ki67- and DCX-immunoreactive cells in the DG was increased in a dose-dependent manner. Especially, the group having 100 mg/kg Gb showed a significant increase of DCX-immunoreactive neuroblasts with well-developed tertiary dendrites. Expression of DCX protein in the Gb groups was also significantly increased upon compared with the vehicle group. The results suggested that repeated intake of Gb would enhance cell proliferation and neuroblast differentiation in the mouse DG.</P>

비강 및 비인두에 발생한 도립유두종과 편평상피암종의 p53단백 및 세포증식능에 관한 연구

한주호,윤기중,이재규,신대균,박근호,조향정,문형배 圓光大學校 醫科學硏究所 1996 圓光醫科學 Vol.12 No.2

The inverted papilloma and squamous cell carcinoma are common neoplasia in the sinonasal cavity and nasopharynx, but the incidence of these tumors are very low and the study on the oncogenesis or biological activity of the tumor cells are not well known. This study was designed to evaluate the oncogenic roles of the p53 gene and the proliferative activity of the tumor cells in the inflammatory polyp, inverted papilloma and squamous cell carcinoma. The experiment was carried by the immunohistochemical stains about the p53 protein, PCNA and Ki-67, histochemical stain about the AgNORs. and flow cytometric analysis about the DNA ploidy using the formalin fixed paraffin embedded tissues. The frequency of the expression of p53 protein was 0%(0/16 cases) in the inflammatory polyps, 18.8% (3/16 cases) in the inverted papillomas, and 87.5%(14/16 cases) in the squamous cell carcinomas. The labelling index(%) of the PCNA and Ki-67 was 5.3% and 3.3% in the inflammatoy polyps, 29.6% and 25.2% in the inverted papillomas, and 51.9% and 36.8% in the squamous cell carcinoma. The expression of the PCNA and Ki-67 was distributed in the periphery of the tumor islands of the inverted papilloma and was distributed in the both of center and periphery of the tumor islands of the squamous cell carcinoma. The number of nuclear AgNORs was increased in the order of inflammatory polyps (0.96), inverted papillomas(1.34) and squamous cell carcinoma(2.09). The frequency of the DNA aneuploidy was 0%(0/16 cases) in the inflammatory polyps, 18.8%(3/16 cases) in the inverted papillomas. and 12.5%(2/16 cases) in the squamous cell carcinomas. Above results indicates that the changes of the p53 gene and proliferative activity of the tumor cells are involved on the oncogenesis and the biological activity of the inverted papilloma or squamous cell carcinoma in the nasopharynx and sinonasa cavity.

안동호의 수역별 영양상태와 평가방법간 상관성 비교

박재충,박정원,김대현,신재기,이희무 한국육수학회 2005 생태와 환경 Vol.38 No.1

안동호를 5~10km 구간으로 분할한 5개 지점에 대하여 2002년 6월부터 2003년 6월(13개월)가지 총 21회에 걸쳐 지점별 물리 화학적 수질특성과 TSI, TSIm, LTSI로 수체의 영양상태 평가 및 평가방법간의 상관성을 비교 분석하였다. TSI와 TSIm은 평가항목(TP, Chl-α, SD)에 따라 지점별로 빈영양-중영양-부영양상태로 다양한 결과를 보여 개별 항목으로 수역의 영양상태를 평가하는 것은 곤란하였으나 댐 저수지의 상류에서 하류로 진행하면서 영양도가 낮아지는 결과는 세 평가방법이 동일하였다. 평가방법 간의 상관성은 수심이 깊고 연중 안정된 수질상태를 보이는 하류보다 수심이 얕고 수질변동 폭이 큰 상류수역에서 상대적으로 높았으며, 영양지수의 평균값에 의한 상관은 r=0.9117~0.9909 범위로 조사되어 상호 보완적인 적용은 가능한 것으로 나타났다. LTSI는 TSI와 TSIm에 비해 댐 저수지의 구간별 수질특성을 잘 반영하였으며 영양상태 평가의 간편성과 주관적인 평가 오류의 최소화에 보다 효과적이라 사료된다. Using TSI, TSIm and LTSI, we compared physico-chemical water quality and trophic state of 5 stations in Lake Andong from June 2002 to June 2003 and examined the correlation of them. It is difficult to evaluate trophic state of waters by each parameter, TP, Chl-α and SD, because TSI and TSIm in each section show different results, oligo-, meso- and eutrophic state. But three methods, TSI, TSIm and LTSI, show the same result that trophic degree lows as it goes to a lower area of the lake. The correlation among them is closer in the upper shallow waters than in the lower deep waters. The reciprocal application of trophic state indices is possible because the coefficient of mean correlation ranges 0.9117 to 0.9909. In conclusion, it seems that LTSI reflects a characteristic of water quality in each section better than TSI, TSIm and is very effective to simplify trophic state evaluation and minimize researcher's subjectivity.

사비의 교정에 있어서 내시경의 이용

박대환,김태모,이재욱,송철홍,한동길,안기영 大韓成形外科學會 1998 Archives of Plastic Surgery Vol.25 No.1

Owing to the complexity of the deviated structures and the septal deformities accompanying the external nasal deformities, the complete correction of the deviated nose is regarded as a difficult one. The approach to the nasal bone and osteotomy in classic corrective rhinoplasty is almost blind technique, where the results depends on the feeling by surgeon's hand. To overcome these drawback, endoscopic-assisted corrective rhinoplasty and septoplasty performed for 8 cases of deviated nose between January 1996 and May 1997. Average follow-up period was 10 months. All patients were evaluated by symmetrical nasal pyramid, recurrence of the bony deflection and septal deviation. The postoperative courses were satisfactory in most of cases with few complications. It appears that endoscopic control during corrective rhinoplasty and septoplasty is a big step toward obtaining better results in bony and cartilage resection with extreme precision under monitor control and magnification. This technique is not an open approach but permits one to see more of the nasal skeleton and bony septum what is causing deformity, and the immediate effect of the corrective measures used. The use of endoscope in corrective rhinoplasty will provide expanded field of vision, direct manipulation of lesions, and better aesthetic an6 functional results.

좌골부 욕창의 치료

박대환,한동길,안기영 대구효성가톨릭대학교 1996 연구논문집 Vol.53 No.2

Ischial ulcers are the most common of all pressure ulcers, probably because they directly overlie the points of maximum pressure on the sitting surface. Furthermore, they are among the most common secondary ulcers for the same reason. Also, ischial ulcers are frequently complicated by abnormal lesional bursae, by extension, fistula toward the perineum, urethra, or peritoneal cavity. Urethrograms or panendoscopy are necessary prior to surgery and must remove all irreversibly damaged tissue. Consequently any surgical plan for ischial ulcers must take into account the effects of bone removal on weight bearing and positioning, and must provide stable, durable soft tissue cover intelligently designed to be reliable in the event of a recurrence. From April 1990 to April 1993, we had reconstructed 15 cases of ischial ulcers. In 13 cases of primary ulcers, inferior gluteil thigh flip (7 case), transpositin flap(2 case), ischiectomy and direct closure (3 cases), gluteus maximus myocutaneous island flap(1 case) was done. In 2 cases of secondary ulcers, V-Y advancement flap of biceps femoris and semitendinosus and gracillis muscle flap were done each other.

백서 허혈성 국소 뇌경색에서의 소뇌 실정핵 자극 효과

박윤관,김대환,정흥섭,이기찬,주정화,이훈갑 대한신경외과학회 1996 Journal of Korean neurosurgical society Vol.25 No.3

Elecrical stimulation of the cerebellar fastigial nucleus(FN) increases cerebral blood flow(CBF) and reduces brain damage after focal cerebral ischemia. The authors studied whether the neuroprotection elicited from electrical stimulation of the cerebellar FN is attibutable to the elevation in regional CBF(rCBF) or reduction in release of excitatory amino acid. Sprague-Dawley rats were anesthetized with a mixture of halothane(3% for the induction and 1% for maintenance) and oxygen, and artificially ventilated through a tracheal cannula. Arterial pressure, blood gases and body temperature were monitored. The middle cerebral artery(MCA) was occluded distal to the lenticulostriate branches. The FN was then stimulated for 1 hour(100uA : 50Hz : 1sec on/1sec off). The changes in rCBF were measured by hydrogen clearance method for 2 hours, over the regions corresponding to the ischemic core and penumbra. Postischemic release of glutamate and aspartate were measured by microdialysis for 2 hours at the same site of measurement of rCBF. Infarct volume was determined 8 hours later in 2, 3, 5-triphenyl tetrazolium chloride(TTC)-stained sections. FN stimulation(n=12) increased mean arterial pressure by 28±16mmHg. In nonstimulated control rats(n=12), mean AP was not changed significantly during the experimental procedures. Compared with nonstimulated animal, stimulation of FN for 1 hour following MCA occlusion significantly increased rCBF in ischemic core and penumbra by 53.6% and 67.6% respectively. And the volume of infarction decreased by 42% at 8 hours after MCA occlusion. The concentration of glutamate and aspartate in ischemic core after MCA occlusion increased both in the control group(to 12.2±3.3 folds and 10.4±4.1 folds, respectively) and in the stimulation group(10.5±2.8 and 11.2±4.1 folds, respectively). The concentration of glutamate and aspartate in penumbra did change significantly neither in the control group(to 2.5±1.3 folds and 1.8±0.6 folds, respectively) nor in the stimulation group(1.9±0.5 folds and 2.1±0.4 folds, respectively). There was no significant difference between the two groups. These data indicate that neuroprotection elicited from electrical stimulation of the erebellar FN is attributable to the elevation in rCBF but no to the reduction in release of excitatory amino acid.

초저온 냉기를 이용한 한냉치료후 동통유발점압통역치의 변화

홍대종,최기섭,박시복,김영호 大韓再活醫學會 1999 Annals of Rehabilitation Medicine Vol.23 No.1

원위부를 기초로 한 도서형 정맥 피판의 임상적 응용

박대환,황동하,안기영 大韓成形外科學會 1994 Archives of Plastic Surgery Vol.21 No.6

Skin flaps are characterised by arterial inflow and venous outflow. Since Baek et al(1985) demonstrated experimentally the possibility of raising a flap based only on a single vein surviving without arterial inflow, experimental and clinical attempts in using the 'flow through' venous flap, proximally pedicled venous flap, and distally pedicled venous flap have been performed and developed. In our study, five flaps with only a distal venous pedicle were carried out; two saphenous venous flap, one cephalic venous flap, one external jugular venous flap, and one dorsal venous flap of hand. Four of these five survived completely without complication but only the external jugular venous flap showed partial necrosis. These flaps are useful because of almost infinite donor site, quicker and simpler technique than free flap, and little donor site morbidity. Further studies are required for improved survival rate of venous flap.

維新벼에 나타난 生育後期의 萎週

朴鍾聲,金文圭,禹基大,劉勝憲 충남대학교 농업과학연구소 1977 農業技術硏究報告 Vol.4 No.1

1. Chemical properties of soils and inorganic compositions of rice plants were analysed to explain the acute wilting occurred in the late stage of rice variety, Yusin. The results obtained were summarized as follows. 1) The amount of K and S_iO_2 of soils where severe rice wilting occurred was lower than that of soils where rice wilting didn't occurred. 2) The amount of N and FeO of wilted rice plants was higher than that of normal rice plants while the amount of K_2O and SiO_2 and the ratio of K_2O/N of former were lower than that of letter. 2. Severe wilting was occurred only in the rice variety, Yusin, when three varieties… Yusin, Milyang #15, Milyang #23……were interplanted. The amount of organic and inorganic compositions of three varieties and chemical properties of rhizosphere soils were analysed. 1) The rice variety, Yusin, was the lowest and that of the rice variety, Milyang #15, was the highest in the level of total carbohydrate and hemicellulose. 2) Comparatively higher levels of K were observed in rhizosphere soil where Yusin was planted, while the level of the K in the plant of Yusin was the lowest.