[환경] 음용 원수의 염소소독에 의한 소독부산물 생성패턴에 관한 연구 Ⅱ

이강진(Kang Jin Lee1),홍지은(Jee Eun Hong),표희수(Heesoo Pyo),박송자(Song-Ja Park),유제강(Je Kang Yoo),이대운(Dae Woon Lee) 한국분석과학회 2004 분석과학 Vol.17 No.1

Predicting postoperative total calcium requirements after parathyroidectomy in secondary hyperparathyroidism

( Byung Heon Kang ),( Soon Young Hwang ),( Jeong Yeop Kim ),( Yu Ah Hong ),( Mi Yeon Jung ),( Eun Ah Lee ),( Ji Eun Lee3 ),( Jae Bok Lee ),( Gang Jee Ko ),( Heui Jung Pyo ),( Young Joo Kwon ) 대한내과학회 2015 The Korean Journal of Internal Medicine Vol.30 No.6

Background/Aims: To prevent hypocalcemia after parathyroidectomy (PTX), parenteral calcium is required in addition to oral calcitriol and calcium. After switching to oral calcium, patients can be discharged from the hospital. The aim of this study was to analyze the clinical characteristics and outcomes of PTX performed at a single Korean center and to investigate the associated laboratory factors used to analyze the total amount of postoperative calcium required. Methods: We enrolled 91 hemodialysis patients undergoing PTX from November 2003 to December 2011. We collected clinical and laboratory data preoperatively, 12 and 48 hours postoperatively, at discharge, and 3 and 6 months postoperatively. Results: In total, 59 patients underwent PTX with autotransplantation (AT), 6 underwent total PTX without AT, 11 underwent subtotal PTX, and 15 underwent limited PTX. Total PTX without AT showed the lowest recurrence rate. At all postoperative time points, the mean levels of serum calcium, phosphorus, and intact parathyroid hormone (iPTH) decreased significantly, compared with preoperative levels; however, alkaline phosphatase (ALP) increased significantly from 48 hours postoperatively to discharge (p < 0.001). On multiple linear regression analysis, the total amount of injected calcium during hospitalization showed a significant correlation with preoperative ALP (p < 0.001), preoperative iPTH (p = 0.037), and Δphosphorus at 48 hours (p < 0.001). We developed an equation for estimating the total calcium requirement after PTX. Conclusions: Preoperative ALP, preoperative iPTH, and Δphosphorus at 48 hours may be significant factors in estimating the postoperative calcium requirement. The formula for postoperative calcium requirement after PTX may help to predict the duration of postoperative hospitalization.

저장 온도 및 상대습도가 비타민 정제 중 지용성 비타민 함량의 변화에 미치는 영향

김동섭,이재황,김세곤,이동언,박석준,이진희,이강표,최성원,백무열 경희대학교식량자원개발연구소 2007 硏究論文集 Vol.26 No.1

한국 정신장애의 역학 조사 연구[I] : 각 정신장애의 유병률

조맹제,함봉진,김장규,박강규,정은기,서동우,김선욱,조성진,이준영,홍진표,최용성,박종익,이동우,이기철,배재남,신정호,정인원,박종한,배안,이충경 大韓神經精神醫學會 2004 신경정신의학 Vol.43 No.4

Objectives : This study aims to estimate the prevalence of the DSM-IV psychiatric disorders in Korean population using the Korean version of Composite International Diagnostic Interview (K-CIDI). Methods : Subjects were selected by taking multi-stage, cluster samples of 7,867 adult household residents, 18 to 64 years of age, in ten catchment areas. Total 78 trained interviewers administered the K-CIDI to the selected respondents, from June 1 to November30,2001. Results : Total 6,275 respondents completed the interview. Some 33.5% of respondents reported at least one lifetime disorder, 20.6% reported at least one-year disorder, and 16.7% reported at least one-month disorder. The most common lifetime disorders were alcohol abuse/dependence (17.24%), nicotine dependence/withdrawal (11.19%), specific phobia (5.16%), and major de-pressive disorder (4.25%). The lifetime prevalence of substance abuse/dependence (0.25%) and schizophrenia (0.16%) was very low. Nicotine and alcohol use disorder showed very high male/female ratio. Mood disorder and anxiety disorder were more prevalent among female than male. Conclusion : The prevalence of psychiatric disorders was high. In comparison with other studies, remarkable differences in distributions of psychiatric disorders across the areas and times were observed.

라면 유지의 안정성에 대한 당과 아미노산의 영향

차경식,이강표,최형택,양 융,오두환 연세대학교 산업기술연구소 1989 논문집 Vol.21 No.1

The effects of various sugars and amino acids of the stability of the plam oil in Ramyon, was studied. Stability of the palm oil in Ramyon was determined from acid, peroxide, anisidine values and color development. The effects of sugars added on the stability of the palm oil in Ramyon was in order of arabinose, xylose, glucose, fructose, lactose, sucrose, raffinose. In addition of amino acids and glucose, the stability of the palm oil in Ramyon showed a significant increase in order of β-alanine, glycine, valine, lysine, but threonine and glutamic acid showed less stability. The effects of glycine concentration on the stability was increased to 0.5%. After deep-fried, color developments of Ramy on surface increased in darkness to 0.5% glycine concentration and decreased during the storage period.

알카리성 부여제 도포에 의한 중성화된 콘크리트의 알카리성 회복성능에 관한 실험적 연구

김무한,권영진,강석표,유재강,이강우 충남대학교 산업기술연구소 2000 산업기술연구논문집 Vol.15 No.1

Concrete is the most commonly used material in construction field and it is the permanent construction material with respect to civil property. But, if concrete loses its alkalinity by CO2 gas, it loses its structural characteristics and results in total collapse in construction field. Therefore. it is needed to be studied the alkali-recovery performance by alkalization agent on carbonated concrete by accelerated carbonation test. In this paper, we investigate recovery performance by impregnating alkalization agent in carbonated concrete, then we evaluate alkali maintenance performance by pH survey in each kinds of finishing materials. The results of this paper can be summarized as follows. 1)Carbonated concrete under pH 10 was recovered its alkalinity to pH 11 at first stage by spreading alkalization agent on its outer surface. 2)After spreading alkalization agent, there is a difference due to types of moreover effect of anti-rust paint and ordinary cement paste were excellent.

장시간 수술시 Propofol과 Enflurane의 마취 유지 효과 및 술후 각성도에 대한 비교

김태요,윤재승,이강창,정영표,안선연 圓光大學校 1995 論文集 Vol.30 No.2

There has not been particular attention focused on the comparative benefits and risks of propofol anesthesia with inhalation anesthesia in the operations of long duration. This study was assessed the anesthetic efficacy and the speed of recovery from propofol or enflurane anesthesia in patients undergoing the long term operations. The propofol group (n=25) receiver 2.0 - 2.5mg/kg propofol intravenously for the induction of anesthesia and followed by propofol infusion(6 - 12mg/kg/h). The enflurane group (n=25) was induced as the propofol group and followed by enflurane(1-2 MAC) addministration. All patients received nitrous oxide (50%) in oxygen immediately after tracheal intubation. All anesthetic agents were stopped at the time of last stitch. The hemodynamic changes were recorded and the recovery was assessed with the time from discontinuation of all anesthetics to extubation and the indices of consciousness at early recovery(recall name, eye opening on command, raise hand on request and coughing on request). The recovery tests showed no differences between the groups. Systolic pressure after intubation in propofol group was significantly increased (p<0.05), compared with enflurane group. In propofol group, seven patients received fentanyl because of light anesthetic depth. The frequency of nausea and vomiting was similar between the groups. In conclusion, the long propofol anesthesia was not associated with faster recovery than enflurane anesthesia and propofol as an agent of anesthetic maintenance was unfavorable.

Janex-1, a JAK3 inhibitor, ameliorates tumor necrosis factor-α-induced expression of cell adhesion molecules and improves myocardial vascular permeability in endotoxemic mice.

Lee, Jung Eun,Lee, Ae Sin,Kim, Duk Hoon,Jung, Yu Jin,Lee, Sik,Park, Byung-Hyun,Lee, Sun Hwa,Park, Sung Kwang,Kim, Won,Kang, Kyung Pyo D.A. Spandidos 2012 International journal of molecular medicine Vol.29 No.5

<P>Vascular endothelial cells play an important role in leukocyte trafficking during the inflammatory process. Proinflammatory cytokines activate the expression of cell adhesion molecules in endothelial cells. Janus kinase (JAK) and signal transducer and activator of transcription (STAT) are important intracellular cytokine signaling molecules that are involved in immune responses. The purpose of this study was to investigate the effect of JAK3 inhibition on the expression of tumor necrosis factor (TNF)-α-induced cell adhesion molecules in vascular endothelial cells and to evaluate the therapeutic potential of JAK3 for myocardial vascular permeability in endotoxemic mice. A JAK3 inhibitor, JANEX-1, decreased the TNF-α-induced expression of intercellular adhesion molecule (ICAM)-1, VCAM (vascular cell adhesion molecule)-1 and fractalkine in human umbilical vein endothelial cells (HUVECs). The downregulation of the expression of these cell adhesion molecules by JANEX-1 was mediated via suppression of STAT3 phosphorylation and nuclear factor-κB (NF-κB) activation. In endotoxemic mice, pretreatment with JANEX-1 prevented not only an increase in the cardiac ICAM-1 expression by LPS in the arteriolar and capillary endothelial cells, but also myocardial vascular leakage. These results suggest that inhibition of the JAK/STAT pathway by JANEX-1 ameliorates the expression of TNF-α-induced cell adhesion molecules in HUVECs and improves myocardial vascular permeability.</P>

Protective role of <small>L</small>-2-oxothiazolidine-4-carboxylic acid in cisplatin-induced renal injury

Lee, Sik,Moon, Sang-Ok,Kim, Won,Sung, Mi Jeong,Kim, Duk Hoon,Kang, Kyung Pyo,Jang, Yong Bum,Lee, Jung Eun,Jang, Kyu Yun,Lee, Sang Yong,Park, Sung Kwang Oxford University Press 2006 Nephrology, dialysis, transplantation Vol.21 No.8

<P><B>Background.</B> Oxidative stress and inflammation are implicated in the pathogenesis of cisplatin-induced nephrotoxicity. <SMALL>L</SMALL>-2-oxothiazolidine-4-carboxylic acid (OTC) is a cysteine prodrug, and increases cellular glutathione (GSH). OTC is converted to cysteine by the intracellular enzyme, oxoprolinase. To date, the protective role of OTC on cisplatin-induced renal injury has not been investigated. The purpose of the present study was to examine the protective effect of OTC on cisplatin-induced renal injury and to examine the mechanism of its protection.</P><P><B>Methods.</B> Mice were treated with cisplatin with or without administration of OTC. The generation of reactive oxygen species (ROS), expression of intercellular adhesion molecule (ICAM)-1 and monocyte chemoattractant protein (MCP)-1 were determined in the kidney using 2′,7′-dichlorofluorescein diacetate, immunostaining or western blot analysis. Nuclear factor (NF)-κB activity, infiltration of F4/80-positive cells and apoptosis were also investigated in addition to renal function and histology using electrophoretic mobility shift assay, immunostaining, western blot analysis, uridine triphosphate (dUTP) nick-end labelling or periodic acid–Schiff staining. The effect of OTC on superoxide dismutase activity and GSH level in cisplatin-treated normal adult human kidney (HK-2) cells were measured using assay kits.</P><P><B>Results.</B> The administration of OTC resulted in a significant reduction of cisplatin-induced ROS production, the p65 subunit of NF-κB translocation into nucleus, expression of ICAM-1, caspase 3 activity, expression of MCP-1 and the infiltration of macrophages into renal tissue. OTC markedly ameliorated renal damage induced by cisplatin through antioxidant and anti-inflammatory effect.</P><P><B>Conclusions.</B> These results suggest that OTC can be a potential therapeutic agent in cisplatin-induced renal injury through decreasing the ROS levels and activation of NF-κB.</P>

Establishment of a platform of non-small-cell lung cancer patient-derived xenografts with clinical and genomic annotation

Kang, Han Na,Choi, Jae Woo,Shim, Hyo Sup,Kim, Jinna,Kim, Dae Joon,Lee, Chang Young,Hong, Min Hee,Park, Seong Yong,Park, A-Young,Shin, Eun Joo,Lee, Seo Yoon,Pyo, Kyoung-Ho,Yun, Mi Ran,Choi, Hun Mi,Lee, Elsevier 2018 Lung cancer Vol.124 No.-

<P><B>Abstract</B></P> <P><B>Background</B></P> <P>Preclinical models that can better predict therapeutic activity in clinical trials are needed in this era of personalized cancer treatment. Herein, we established genomically and clinically annotated patient-derived xenografts (PDXs) from non-small-cell lung cancer (NSCLC) patients and investigated whether these PDXs would faithfully recapitulate patient responses to targeted therapy.</P> <P><B>Methods</B></P> <P>Patient-derived tumors were implanted in immunodeficient mice and subsequently expanded via re-implantation. Established PDXs were examined by light microscopy, genomic profiling, and in vivo drug testing, and the successful engraft rate was analyzed with the mutation profile, histology, or acquisition method. Finally, the drug responses of PDXs were compared with the clinical responses of the respective patients.</P> <P><B>Results</B></P> <P>Using samples from 122 patients, we established 41 NSCLC PDXs [30 adenocarcinoma (AD), 11 squamous cell carcinoma (SQ)], among which the following driver mutation were observed: 13 EGFR-mutant, 4 ALK-rearrangement, 1 ROS1-rearrangement, 1 PIK3CA-mutant, 1 FGFR1-amplification, and 2 KRAS-mutant. We rigorously characterized the relationship of clinical features to engraftment rate and latency rates. The engraft rates were comparable across histologic type. The AD engraft rate tended to be higher for surgically resected tissues relative to biopsies, whereas similar engraft rates was observed for SQ, irrespective of the acquisition method. Notably, EGFR-mutants demonstrated significantly longer latency time than EGFR-WT (86 vs. 37days, P = 0.007). The clinical responses were recapitulated by PDXs harboring driver gene alteration (EGFR, ALK, ROS1, or FGFR1) which regressed to their target inhibitors, suggesting that established PDXs comprise a clinically relevant platform.</P> <P><B>Conclusion</B></P> <P>The establishment of genetically and clinically annotated NSCLC PDXs can yield a robust preclinical tool for biomarker, therapeutic target, and drug discovery.</P> <P><B>Highlights</B></P> <P> <UL> <LI> We established 41 NSCLC PDXs by directly implanting tumor specimens of patients. </LI> <LI> These established PDXs were genetically and clinically annotated. </LI> <LI> The clinical response was recapitulated by PDXs. </LI> <LI> PDXs is a robust tool for biomarker, therapeutic target, and drug discovery. </LI> </UL> </P>