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중합효소연쇄반응을 이용한 Helicobacter pylori검출
최태열,박경남,강정옥,서일혜 대한감염학회 1997 감염 Vol.29 No.5
배 경 : H.pylori는 위염, 위궤양 재발과 밀접한 관계를 갖고 있기 때문에 검사실적 진단은 정확하여야 한다. H. pylori의 세균배양이 가장 바람직하지만 시설이나 노력면에서 많은 어려움이 있다. 이에 저자는 H. pylori의 세균배양과 더불어 PCR 을 실시하여 PCR의 유용성을 판단코져 다음과 같은 실험을 실시 하였다. 방 법 : 위장 장애를 호소하여 위내시경과 생검을 실시한 247명의 병리조직소견에 따라 정상대조군(57명), 만성위염(131명), 활동성만성위염(19명), 만성궤양(8명), 위암(32명) 으로 분류하였다. 세균배양은 brain heart infusion egg yolk agar선택배지를 사용하였고 PCR은 usease A gene sequence 를 증폭할수 있는 primer 2쌍을 선택하여 nested PCR 을 실시하였다. 결 과 : 세균배양에 의한 H. pylori의 검출율은 100명(40%) 였으며, PCR 에 의한 H.pylori의 검출율은 179명(72%) 으로 PCR 법이 세균배양법보다 검출율이 높았다(P:<0.05, Chi-square test, SPSS, ver7.0, USA). 세균배양과 PCR 모두 음성이 68명, 모두양성이 100명, 세균배양음성 PCR양성이 79명이였으며 PCR의 예민도는 0.1pg DNA(1 bacterial cell)였다. 일반세균을 이용한 특이도 검사에서 양성인 예는 없었다. 결 론 : 상기 결과로 미루어 보아 위생검조직에서 H.pylori의 검출은 연구자들이 사용한 PCR 법이 세균배양법보다 신속 정확하였다. Background : Helicobacter pyloir has been implicated in the pathogenesis of active chronic gastritis and peptic ulcer disease in man. Thus, diagnosis and treatment of H. pylori infection are now of growing importance in ucle management. A variety of noninvasive and invasive methods have been described for the detection of H. pylori, but all of these techniques have disadvantages such as time consuming or insensitivity. So we describe the polymerase chain reaction(PCR) assay for the sensitive and specific detection of H. pylori. Methods : Gastric biopsy specimens were obtained from 147 patients undergoing endoscopic examinations at Hayang University Hospital. One half of the specimen was processed for routine culture and the other half for PCR. Bacterial genomic DNA from gastric biopsies are extracted by Instagene. Two sets of primer pairs derived from the nucleotide sequence of the urease A gene of H. pylori were used. Result: H. pylori was cultured in 100(40%) cases and PCR assay deteted 179(72%) cases (P<0.05, Chi-square test, SPSS ver. 7.0, USA). Culture and PCR-positive cases totalled 100, and there were 68 cases negative by both mothods. There were 79 culture-negative and PCR-positive cases, but non that were culture-positive and PCR-negative. The assay was sensitive for as little as 0.1 pg of DNA (1 bacterial cell). The specificity of detection was confirmed by ensuring that the primers did not amplify DNA extract from other bacteria. Conclusion: The PCR is rapid, accurate, and sensitive method for the detection of H. pylori.
톨루엔을 포함한 유기용제의 직업적 폭로로 인한 신경내분비계 영향
이채언,이종태,정의화,손혜숙,문덕환,전진호,강정학,이창희,김휘동,김종한,정귀옥 大韓産業醫學會 1995 대한직업환경의학회지 Vol.7 No.2
Long term occupational exposure to solvent mixtures may cause adverse effects to the central nervous system with neurobehavioral symptoms. And some organic slovents have been suggested to cause impairment of tuberoinfundibular dopaminergic activity and neurochemical mechanisms controlling pituitary secretion. For the purpose of assessing neuroendocrine effects in occupational solvents exposure, hormonal study settings (shoes-manufacturing industry & fishing products industry) and compared with nonexposed controls(33 men, 85 women). Male workers exposed to solvent mixtures had significantly lower plasma level of follicle-stimulating hormone(FSH) than nonexposed male controls. While female workers exposed to solvent mixtures had significantly lower plasma levels of growth hormone(GH), and thyroid-stimulating hormone(TSH) than nonexposed female controls. The results of significant decrease in plasma concentrations of pituitary hormones(FSH, GH, and TSH) in workers exposed solvent mixtures indicate that occupational exposure to solvent mixtures may induce neuroendocrine effects through an effect on hypothalamic pituitary axis.
이정한 ( Jung Han Lee ),김승룡 ( Seung Ryong Kim ),문영진 ( Young Jin Moon ),황정혜 ( Jung Hye Hwang ),성영모 ( Young Mo Sung ),강정옥 ( Jung Oak Kang ),호정규 ( Jeong Kyu Hoh ) 대한산부인과학회 2001 Obstetrics & Gynecology Science Vol.44 No.7
In Korea, malaria is known to have been eradicated for several years due to our persistent national health programs. But recently imported malarias through increased traveling to abroad are becoming a problem and occasional malarial infections are being reported. No malarial infection of pregnant woman has been reported until now, but recently, we have experienced one case of malaria infected pregnant woman who has no history of traveling and blood transfusion. This patient has been treated with hydroxychlorquine. We present this case with a brief review of literatures.
헬리코박터 관련 소화성궤양 환자에서 위산펌프길항제 사용이 요소호기검사 결과에 미치는 영향
옥주현 ( Ju Hyun Oak ),정우철 ( Woo Chul Chung ),정성훈 ( Sung Hoon Jung ),최강현 ( Kang Hyun Choi ),김은정 ( Eun Jung Kim ),강봉구 ( Bong Koo Kang ),강보라미 ( Bo Ra Mi Kang ),공시은 ( Si Eun Kong ),백창렬 ( Chang Nyol Paik ),이 대한소화기학회 2011 대한소화기학회지 Vol.57 No.1
Background/Aims: Revaprazan (Revanex(R)) is a novel proton pump inhibitor (PPI) that has a somewhat different effect on proton pump compared with the other PPI`s, also (called as `acid pump antagonist`). We aimed to examine the false negative rate of (13)C-urea breath test (UBT) in the patients with Helicobacter pylori (H. pylori) associated peptic ulcer disease who were treated with revaprazan and evaluate the anti-urease activity of revaprazan. Methods: Total 55 patients were enrolled in this study. They received EGD examination between January 2009 and December 2009 and diagnosed histologically as H. pylori associated peptic ulcer disease. All patients took revaprazan only. Three patients were excluded because of underlying chronic disease and inappropriate breath sampling. The remaining 52 patients had UBT at 0, 2 and 4 weeks of revaprazan use. After 2 weeks of the cessation of revaprazan, they had the fourth UBT. Results: At 2 and 4 weeks, the false negative rates of UBT were 5.8% and 23.1%, respectively (p=0.05). After 2 weeks of the cessation, the cases of the false negative result were five. Four out of five patients had prolonged negative results on two or three successive tests, and baseline (13)C difference value did not predict the false negative results. Conclusions: False negative results of UBT were common and increased with prolonged use of acid pump antagonist. As PPI, it had also anti-urease activity and most patients (47/52, 90.4%) reverted to positive results by 2 weeks after the cessation of taking the medication. (Korean J Gastroenterol 2011;57:8-13)