Paricalcitol attenuates lipopolysaccharide-induced inflammation and apoptosis in proximal tubular cells through the prostaglandin E₂ receptor EP4

( Yu Ah Hong ),( Keum Jin Yang ),( So Young Jung ),( Yoon Kyung Chang ),( Cheol Whee Park ),( Chul Woo Yang ),( Suk Young Kim ),( Hyeon Seok Hwang ) 대한신장학회 2017 Kidney Research and Clinical Practice Vol.36 No.2

Background: Vitamin D is considered to exert a protective effect on various renal diseases but its underlying molecular mechanism remains poorly understood. This study aimed to determine whether paricalcitol attenuates inflammation and apoptosis during lipopolysaccharide (LPS)-induced renal proximal tubular cell injury through the prostaglandin E₂ (PGE₂) receptor EP4. Methods: Human renal tubular epithelial (HK-2) cells were pretreated with paricalcitol (2 ng/mL) for 1 hour and exposed to LPS (1 μg/mL). The effects of paricalcitol pretreatment in relation to an EP4 blockade using AH-23848 or EP4 small interfering RNA (siRNA) were investigated. Results: The expression of cyclooxygenase-2, PGE₂, and EP4 were significantly increased in LPS-exposed HK-2 cells treated with paricalcitol compared with cells exposed to LPS only. Paricalcitol prevented cell death induced by LPS exposure, and the cotreatment of AH-23848 or EP4 siRNA offset these cell-protective effects. The phosphorylation and nuclear translocation of p65 nuclear factor-kappaB (NF-κB) were decreased and the phosphorylation of Akt was increased in LPS-exposed cells with paricalcitol treatment. AH-23848 or EP4 siRNA inhibited the suppressive effects of paricalcitol on p65 NF-κB nuclear translocation and the activation of Akt. The production of proinflammatory cytokines and the number of terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling-positive cells were attenuated by paricalcitol in LPS exposed HK-2 cells. The cotreatment with an EP4 antagonist abolished these anti-inflammatory and antiapoptotic effects. Conclusion: EP4 plays a pivotal role in anti-inflammatory and antiapoptotic effects through Akt and NF-κB signaling after paricalcitol pretreatment in LPS-induced renal proximal tubule cell injury.

병원식당 직원급식을 위한 조리방법의 개선 : 직원급식에 종사하는 조리원을 대상으로

나영아,윤은숙,김정환 서울보건대학 1996 서울보건대학 부설 병원경영연구소 논문집 Vol.2 No.1

This study was carried out to evaluate hospital cook`s cooking method and to suggest the more proper food-service guideline for hospital staff. Questionnaire method to hospital cooks were practiced and the data were analyzed by SAS package program for the frequency, percentage, Anova and the Duncan multiple test. The summarized results are as like these ; 1. Hospital cooks' recognition degree for kitchen management was very excellent. 2. Hospital cooks who are in charge of hospital staff were very positive to participate cooking method improvement program. 3. The more careered cook, the more sincerely have done for standard recipe in hospital kitchen. 4. The degree of cooks` desire for cooking method education programs was indicated by significance.

안모의 수직적 양태에 따른 골격 및 치조골의 위치 관계에 관한 연구

오중식,국윤아,김상철 대한치과교정학회 1999 대한치과교정학회지 Vol.29 No.3

교정 증례의 분석 예후 추정은 두개골의 수평 및 수직적인 관계가 동시에 고려되는 입체적 관점에서 평가되어야 한다. 따라서 본 연구에서는 성장이 완료된 성인 남녀 각 100명을 추출하여 두부 방사선 사진을 채득한 후 하안면 고경과 상안면 고경의 비율로 안모의 수직적 수직적 형태를 구분하여 군을 설정하였으며 이들 군간의 골격 및 치조골의 크기와 위치 관계를 비교하고 상호 관련성을 조사하여 다음과 같은 결과를 얻었다. 1. 치조고경, 안면 고경, lower gonial angle, 그리고 FMA 항목에서 하안면 고경이 큰 군의 것이 작은 군에 비하여 유의하게 크게 나타났다. 2. 치조고경, 안면고경, 하악지 고경, 그리고 Jarabak ratio 항목에서 남서이 여성에 비하여 유의하게 크게 나타났다. 3. 치성 및 골격성의 수직계측항목과 UAFH/LAFH의 상관관계 조사에서 치조고경,안면고경, lower gonial angle, FMA 그리고 Bjok's Sum 항목이 UAFH/LAFH와 유의한 상관관계를 보였다. The purpose of this study was to investigate the variations of the skeletal and dentoalveolar dimensions in relation to vertical facial patterns. Lateral cephalogram of 200 cases (100 cases of male and 100 cases of female, average age of which was 23.2 years) were traced and some measurements of skeletal and dentoalveolar dimensions were measured. The ratio of UAFH/LAFH was employed to classify the samples into groups of excess and short lower anterior facial height. And the comparison between two groups were taken statistacally. The following results were obtained. 1. The dentoalveolar height, lower anterior facial height, lower gonial angle, and FMA in the excess-lower-anterior-facial-height group were significantly larger than those in short-lower-anterior-facial-height group. 2. The dentoalveolar height, ramus height, and Jarabak ratio in the male subjects were significantly larger than those in the female subjects. 3. The UAFH/LAFH ratio showed a significant correlation to upper, lower facial height, AUDH, PUDH, ALDH, PLDH, Lower gonial angle, FMA, and Bjork's Sum.

한국인에서 구강상피세포를 이용한 발암물질 대사효소의 유전자 다형성에 관한 연구

최민아,박해준,정원윤,이재원,이정복,박광균 대한암예방학회 2002 Journal of cancer prevention Vol.7 No.3

한국 청소년에서 혈청 Adiponectin 농도와 비만도와의 상관관계

김현아,이형숙,김철식,안철우,정윤석,이관우,허갑범,김대중 대한내분비학회 2003 Endocrinology and metabolism Vol.18 No.5

연구배경: Adiponectin은 지방세포에서 특이적으로 발현되고 분비되는 adipocytokine이다. 아직 adiponectin 과 소아 비만과의 관련성을 연구한 보고는 많지 않다. 본 연구에서는 한국 청소년을 대상으로 혈청 adiponectin농도와 비만도 및 심혈관계 질환의 위험인자들과의 상관관계를 보고자 하였다. 방법: 2003년 4월 서울특별시 서대문구 소재의 한 중학교에서 12∼15세의 무작위 선발된 164명을 연구 대상으로 하여 신장, 체중, 허리둘레, 체지방을 측정하고 체질량지수를 계산하였다. 또한 공복채혈을 통하여 콜레스테롤, HDL-콜레스테롤, 중성지방, 혈당 및 인슐린 농도를 측정하고 혈청 adiponectin 농도는 ELISA 방법을 이용하여 측정하였다. 결과: 혈청 adiponectin 농도에 따라 삼등분하여 저 adiponectin군, 중간 adiponectin군, 고 adiponectin군으로 나누고 각 군간에 신체계측치 및 임상적 특성을 비교 분석하였다. 저 adiponectin군에서 다른 군에 비해 체질량지수와 허리둘레가 의미있게 크게 나타났다. 또한 저 adiponectin군에서 공복혈당 및 공복혈청 인슐린 농도가 높게 나타났으며, HOMA-IR 값도 높게 나타났다. 저 adiponectin군에서 총콜레스테롤과 중성지방치는 높게, 그리고 HDL 콜레스테롤치는 낮게 나타났다. 다변량회귀분석을 시행한 결과 혈청 adiponectin농도와 가장 상관관계가 높은 변수는 허리둘레와 성별로 나타났다. 결론: 소아에서도 성인과 마찬가지로 혈청 adiponectin농도와 복부비만 및 심혈관계 질환의 위험인자들과의 강한 음의 상관관계를 보임을 알 수 있었다. 향후 사춘기 성호르몬의 변화가 adiponectin에 미치는 영향 및 소아비만의 치료에 따른 adiponectin의 변화 등에 대한 연구가 필요하리라 사료된다. Background: Adiponectin is an adipocytokine that is highly specific to adipose tissue. In contrast to other adipocytokine, the adiponectin levels are decreased in obesity and/or type 2 diabetes. There are few studies regarding the correlation between the adiponectin concentration and obesity in children. Thus, whether the serum adiponectin concentrations are associated with adiposity in children was investigated. Methods: One hundred and sixty four subjects were selected from the participants in an ongoing study on the relationship between birth weight and insulin resistance in children. The current weights, heights, body fat percentages, waist circumferences, blood pressures, lipid profiles and insulin resistance, by the HOMA method, were measured in all the subjects. The serum adiponectin concentrations were determined by a validated sandwich ELISA, using a human adiponectin-specific antibody. Results: The serum adiponectin concentration was negatively correlated with the body mass index, waist circumference, systolic blood pressure, insulin resistance by HOMA and serum triglycerides, and positively correlated with the serum HDL cholesterol level. The serum adiponectin concentrations in the boys were significantly lower than in the girls. In a multiple regression analysis, the serum adiponectin concentration was strongly associated with waist circumference and gender. Conclusion: It is concluded that there was an inverse relationship between the serum adiponectin concentration and abdominal adiposity in children. However, further studies on independent gender differences on adiponectin are needed (J Kor Soc Endocrinol 18:473∼480, 2003).

Pesticide Residue Monitoring Program in Korea, 2005-2008

Jung Ah Do,Yoon Jung Kang,Hyoung-Joon Park,Moo Song Lim,Woo Seong Kim,Chunsun Moon,Do Hoon Kim,Chansoon Kang,Sun Hee Lee,Dae Hyun Cho 한국농약과학회 2008 한국농약과학회 학술발표대회 논문집 Vol.- No.-

Production of fermentable sugars using carbohydrolytic enzymes from citrus peel degrading fungi

Ah Reum PARK,Choi Jung YOON,Jeong Hoon PARK,Joseph SONG,Jung-Hoon SOHN,Jeong-Jun YOON 한국생물공학회 2011 한국생물공학회 학술대회 Vol.2011 No.10

Activation of peroxisome proliferator-activated receptor δ (PPARδ) accelerates blastocyst hatching in mice

Jung Ah Yoon,Hee Jung Kang,Soo Jin Hwang,Hyunjung Lim,Haengseok Song 한국발생생물학회 2011 한국발생생물학회 학술발표대회 Vol.30 No.-

In particular, maternal prostacyclin (PGI2) is critical for embryo implantation and the action of PGI2 is not mediated via its G protein-coupled membrane receptor, IP, but its nuclear receptor, peroxisome proliferator-activated receptor δ (PPARδ). Recently, several studies have shown that PGI2 enhances blastocyst development and/or hatching rate in vitro, and subsequently implantation and live birth rates in mice. However, the mechanism by which PGI2 improves preimplantation embryo development in vitro remains unclear. Using molecular, pharmacologic and genetic approaches, we show that PGI2-induced PPARδ activation accelerates blastocyst hatching in mice. mRNAs for PPARδ, RXRs (heterodimeric partners of PPARδ) and PGI2 synthase are temporally induced after zygotic gene activation and their expression reaches maximum levels at the blastocyst stage, suggesting that functional complex of PPARδ can be formed in the blastocyst. Carbaprostacyclin (cPGI, a stable analogue of PGI2) and GW501516 (a PPARδ selective agonist) significantly accelerated blastocyst hatching but did not increase total cell number of cultured blastocysts. Whereas U51605 (a PGIS inhibitor) interfered with blastocyst hatching, GW501516 restored U51605-induced retarded hatching. In contrast to improvement of blastocyst hatching by PPARδ agonists, PPAR antagonists significantly inhibited blastocyst hatching. Furthermore, deletion of PPARδ at early stages of preimplantation mouse embryos caused delay of blastocyst hatching, but did not impair blastocyst development. Taken together, PGI2-induced PPARδ activation accelerates blastocyst hatching in mice.

Low-temperature Cu(I) loading on a mesoporous Metal–Organic framework for adsorptive separation of C₃H₆/C₃H₈ mixtures

Yoon, Jung Woon,Kim, Ah-Reum,Kim, Min June,Yoon, Tae-Ung,Kim, Jeong-Hoon,Bae, Youn-Sang Elsevier 2019 Microporous and mesoporous materials Vol.279 No.-

<P><B>Abstract</B></P> <P>We incorporated Cu(I) ions into a mesoporous metal–organic framework (MOF) through facile method involving CuCl<SUB>2</SUB> loading, followed by reduction using Na<SUB>2</SUB>SO<SUB>3</SUB> as reducing agent. The successful incorporation of Cu(I) ions was supported by various methods such as TEM-EDS, XPS and ICP-AES. Remarkably, the Cu(I) incorporation resulted in 4.2 times higher C<SUB>3</SUB>H<SUB>6</SUB>/C<SUB>3</SUB>H<SUB>8</SUB> selectivity compared with the parent MOF as well as large C<SUB>3</SUB>H<SUB>6</SUB> working capacity (1.3 mmol/g) at 10–100 kPa. Moreover, the Cu(I)-loaded MOF showed efficient separation of C<SUB>3</SUB>H<SUB>6</SUB> from C<SUB>3</SUB>H<SUB>8</SUB> under dynamic mixture flow conditions, easy regeneration under mild conditions, and good stability under atmospheric conditions.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Cu(I) was loaded into a mesoporous MOF at low temperatures without acid or base. </LI> <LI> Cu(I)-loaded MOF has a large C<SUB>3</SUB>H<SUB>6</SUB> working capacity and high C<SUB>3</SUB>H<SUB>6</SUB>/C<SUB>3</SUB>H<SUB>8</SUB> selectivity. </LI> <LI> Cu(I)-loaded MOF shows efficient separation under dynamic mixture flow conditions. </LI> <LI> Cu(I)-loaded MOF is easy to regenerate under mild conditions. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

[Colorectal] Impact of Postoperative Bowel Function on Quality of Life for Patients with Rectal or Rectosigmoid Junction Cancer

Yoon Ah Park,Woo Yong Lee,Seong Hyeon Yun,Hee Cheol Kim,Yong Beom Cho,Jung Wook Huh,Jung Kyong Shin,Su Yeon Song 대한외과학회 2022 대한외과학회 학술대회 초록집 Vol.2022 No.11