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      • KCI등재

        Trichoderma viride QM 9414가 生産하는 Cellulase 特性에 관한 硏究

        윤은숙,이혜정 한국식품영양학회 1990 韓國食品營養學會誌 Vol.3 No.1

        In order to obtain the fundamental informations on cellulase of Trichoderma viride QM 9414 for its production and utilization, some physico-chemical properties of the enzyme were reviewed. When T. viride QM 9414 was cultured on wheat bran medium, filter paper-disintegrating and carboxymethyl cellulose(CMC)-saccharifying activity were incresed with the cell growth, and thereafter CMC-saccharifying activity was kept on almost the same leved while filter-paper disintegrating activity was decreased sharply. And β-glucosidase was formed maximally on the late stationary phase of growth. The crude cellulase of cell-free extracts was purified by (NH_4)_2SO, fractionation, Sephadex-G 200 column chromatography and DEAE Sephadex A-50 column chromatography. Filter paper-disintegrating, CMC-saccharifying and β-glucosidase activity were purified 10-fold, 47-fold and 38-fold, respectively. The crude enzyme was proved to be a complex of three different enzyme proteins which were showing filter paper-disintegrating, CMC-saccharifying and β-glucosidase activity. The optimal pH of the three enzyme components was alike pH 4.0, and the optimal temperature for CMC-saccharifying, filter paper-disintegrating and β-glucosidase activity were 40℃, 45℃ and 50℃ respectively. The Km and Vmax values of CMC saccharifying activity for CMC were 0.485% and 3.10(μmole/min·㎖), and the Km and Vmax values of β-glucosidase for ONPG(o-nitrophenol glucopyranoside) were 0.944×10 exp (-3)M and 0.097(μmole/min·㎖), respectively. The Km and Vmax values of filter paper-disintegrating activity for Avicel were determined to be 0.09% and 0.178(μmole/min·㎖), respectively. β-Glucosidase activity was competitively inhibited by glucose, and the Ki value for this enzyme was 3.54×10 exp (-3)M, CMC saccharifying activity was found to be greatly inhibited by cellobiose.

      • 看護學生의 價値觀에 關한 調査

        尹銀淑 순천청암대학 1978 論文集-順川看護專門大學 Vol.4 No.-

        This investigation is carried out on the assumption that students will be derived through careful analysis of their attitudes toward various problems in their life space. Results of this investigation are as follows; about two thirds of them showed the positive and affirmative attitude for the human life, and the aims of life of most of them to be achieved certainly were the "cultivation of personality." and the "happy home life." More than half of the students surveyed answered that they would feel the happiest when their aims are achieved and the main ingredient of their current concern and uneasiness was their future problem (Job probem) the majority of the students surveyed believed that the indispensable condition for success was personal efforts. Most of the students believed that the most important in life was happiness and love and health is the next. More than half of the students through that a wise mother and a good wife are the most desirable female.

      • 一部 看護學校 學生들의 特性과 問題點 改善을 위한 調査硏究

        尹銀淑 순천청암대학 1978 論文集-順川看護專門大學 Vol.4 No.-

        A study for inprovement of Nusing Students Traits and problem points by Eun sook Yoon Soon chun nursing Junior college. 1. Their interest on our soeiet is neutral but Their keen observation is reflected when they pointed out some aspects of society as "Sexual Life". Mainly their attention is centered around "Corruption" next is "economic aspect", and "edueational aspect" come in order. 2. The majority of the students survered had religion, and Christianity was prominent. The motives for having a religion varied; to cultivate personality, by other's persuation; to follow others, by influenee under the family background. 3. Parents'expection toward their chilbren is reported "suitable." This makes us feel that there is no severe psychological tension between parents & children. There seems to be, however, some problematic families when approximately 13.9 percent of students report their parents "heavy expectation Toward them." Generally, students tend to have one-hour Conversation with parents each day. but less than 30 minutes. 4. It makes us surprise that remarkably high ratio of students report of having no experience of date with oppositie sex Main reasons; ① Unable to find a suitable partner and ② no opportunity. wheras students having an experience of date point out main motives; ① naturally at some meeting and ② by chance.

      • KCI등재

        14∼15세기 우량카이 3衛와 몽골․明 관계

        윤은숙 명청사학회 2015 명청사연구 Vol.0 No.43

        Üriyangqai was closely connected with Mongolia to the north. And, Üriyangqai had relations with the Ming's governing areas to the south to connect Koryo and Jurchen force so that Üriyangqai 3-Wi's direction had important role at confrontation between the Ming and Mongolia. A study on the roles between Mongolia and the Ming could be base of the analysis upon political situation in East Asia in the 15th century. In Mongolia, Üriyangqai was called ‘Ölu Tuman’ that ‘Ölu’ indicated southern slope of the Xinganling Mountains where the Dongbangwangga expanded feudal estate site since being given feudal estate from Chinggis Khan, and that meant Manho Group to the south of Xinganling. Ming provided Üriyangqai with enormous amount of economic advantages by prizes and foreign trade to investigate political situation and military trends of Mongolia by using their eyes and ears. Üriyangqai's information played an important role at Ming's policy against Mongolia. Üriyangqai 3-Wi gave Ming schedule of Mongolia upon Ming's needs to be given economic advantages from Even if Ming. Ming opened Üriyangqai at the surrender of Dongbangwangga, Üriyangqai 3-Wi followed political system and society of Mongolia. Üriyangqai that was belonged to Mongolia was not governed completely by Mongolia. Üriyangqai that was Ölu with decentralization was rather independent and decentralized when the central government did not control enough: So, Üriyangqai accepted changes of political situation of Mongolia sensitively to unite and have hostility when necessary to have their own areas.

      • 個人衛生의 知識 및 實踐에 關한 調査硏究 : 一部 國民學校 六學年學生을 對象으로

        尹銀淑 순천청암대학 1979 論文集-順川看護專門大學 Vol.5 No.-

        The purpose of this survey is to know the dfference between the degree of knowledge and practice of pesonal hygiene of primary school's sixth grade students. This data(necessary for purpose)were obtained kowledge and practice of personal hygiene of sixth grade students of primary schools witch Iocated in urban and rural from september 15.to 30.1979 The ToTal students witch responded the fuestionair are 500 Pupils. As the result, followlng Conclusions were obtained. 1. Occupation of student's house holder consisted most frefuently of agriculture 38.6% 2. Financial or Living standard in the opinion of students were "less than ordinary" or "Poor" with 15% of the students. 3. Number of tooth cleaning per day:The highest propotion of tooth clcaning was Three times per day,44.8% in the knowledge while in the practice once per day, 63.0 % 4.Hour of tooth cleaning per day: In the knowledge of the highest propotion cleaniny before meal was 36.8% 5.Handwashing after stooh : In the knowledge, wasting regulary was the hyhest regulary was also first,56.8% and never was 7.0% 6.Hand wasting before meal: In the knowledge, regulary before meal was the highest propotion 91.0 % but in the practice, washing regulary was 5.2% 7.Freguan cies of an THelm intics administration: In the knowledge, Twice per year was the most case, 52.4% and in the practice Twice per year was also first, 42.0% 8.Number of bath: In the knowledge, once a week was the highest propotion, 74.0% and in the practice,onee a week was the highest 52.8% 9.Freguency of changing under-wear: In the knowledge, once per 3∼4 days was the First,61.0% and in the practice, once a week was also first 57.8% 10. Utilization of medical facilitles. The 73.4% of the students was utilized the hospital in the knowledge, while in the practice,61.4% of the students were utilized the pharmacy 11. Medical examination: In the knowledge, fo take regulary medical examination was the respond, 79.6% while in the practice:the highest propotion diln't take regulary medical examination 65.6% 12. The reason why They do practice though They have a good knowledge about personal hygiene:The hyhest propotion they were unwillingly 41.8%. The second reason was that they have not health facility and economic foundation. 13.Knowledge of the public nuisance: The percentage of they dont know the public nuisanee was 9.6% 14. Source of information about public nuisance: They know it from Teacher 45.4% the scond from T.V 28.6%

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