HLA-DPB1 유전자 형별법 개발

김태규,이혜정,정서영,정태준,한훈 대한조혈모세포이식학회 1997 대한조혈모세포이식학회지 Vol.2 No.1

연구배경: HLA-DP 유전자는 DR 및 DQ와 같이 항원제공 세포(APCs), B 세포 및 활성화된 T 세포등의 표면에 발현되는 class Ⅱ 유전자로서 외인성 항원제공시 MHC 제한(restriction)과정을 통해 면역 반응에 관여한다. DP 항원 분자는 PLT(primed lymphocyte test)와 같은 세포학적 방법에 의해 처음으로 규명되었고, 이어서 혈청학적 형별이 시도되었으나, DR, DQ 분자에 비해 발현 정도가 낮아 형별에 어려움이 있었다. 최근 DP 유전자의 염기서열이 밝혀짐에 따라 DNA 수준에서 DP 형별이 가능하게 되었으며, 지금까지 DPA1 10개, DPBI 77개의 대립유전자가 밝혀졌다. 본 연구는 이식의 결과 및 질병 발생과 연관이 있다고 추정되는 HLA-DPB1 대립유전자를 DNA 수준에서 형별하고 정상 한국인의 DP 대립유전자 분포를 확인하기 위해 시도되었다. 방법: HLA-DPB1 유전자 중 초가변부위(hypervari-able region)가 있는 exon 2 부위를 일차적으로 증폭시킨후, 17종의 probe를 사용하여 PCR-SSOP를 실시하였고, PCR-SSP에는 5종의 primer를 사용하였다. 이들 방법은 DP형이 알려진 미국 UCLA 대학의 international cell exchange sample 88개를 표준 DNA 시료로 이용하여 확립하였다. 결과: 36종류의 HLA-DPBI 대립유전자를 형별할수 있음을 확인하였다. 그러나 DPBI*0402와 0601 대립유전자를 동시에 갖는 heterozygote인 경우에는 DPBI*0201와 2001을 동시에 갖는 heterozygous와 구분이 어려웠다. 100명의 정상 한국인에서 DPBI 대립 유전자형의 분포를 조사한 결과 한국인에서는 11종류의 HLA-DP 대립유전자가 존재함을 확인하였고, DPBI*0501(36.5%), 0201(27%), 0402(10.5%) 대립유전자의 순으로 분포되어 있었으며 검출율은 100%였다. 결론: 이상의결과로 골수 이식 등 이식시 공여자와 수혜자간의 조직적합성은 HLA-DP, DQ뿐만 아니라 DP도 확인 하게되었으며, 앞으로 질병과의 연관성 연구에서 이용될수 있을것으로 사료된다. Background: The HLA-DP genes are highly polymorphic, which encode heterodimeric cell surface glycoproteins that play a role in the immune response as restriction elements in antigen presentation. HLA-DP antigens were initially defined through the primed lymphocyte test (PLT), and the serological typing has been performed which is practically difficult because the expression of DP molecules is very low, comparing with that of DR and DQ. Recently, DNA sequencing and PCR have allowed the various and extensive study on the HLA-DP genes. We developed the molecular typing method for HLA-DPBI alleles, and studied the distribution in normal Korean population. Methods: After PCR amplification of hypervariable exon 2 regions in HLA-DPBI gene, both PCR-SSOP (sequence specific oligonucleotide probes) with 17 probes and PCR-SSP (sequence specific primers) by 5 Primers were used. And this method was tested using a international pannel DNA as standard. Results: Total 36 alleles of HLA-DPBI were defined in the standard DNA pannel. However, DPBI*0.402, and 0601 heterozygote could not be distinguished from DPBI*0201 and 2001 heterozygote. And 11 alleles were defined in 100 normal Koreans and the common alleles were DPBI*0501 (36.5%). 0201 (27%) and 0402 (10.5%). The detection rate was 100% in this study. Conclusion: As the results, the molecular typing of HLA-DPBI alleles is possible for the accurate matching between donor and recipient in born marrow transplantation and for the study of disease association.

전부도재관용 레진시멘트의 생체적합성에 관한 연구 : Ceramic Crown

김광준,진태호 원광대학교 치의학연구소 2003 圓光齒醫學 Vol.12 No.1

This study was investigated to evaluate the biocompatibility of resin cements for all- ceramic crowns. The resin cements used in this study were Panavia F (Kuraray Co., Ltd. Japan), Variolink Ⅱ(Vivadent Ets., Schann / Liechtenstein), and Bistite Ⅱ(Bistite dual cure resin cement-clear, Tokuyama Soda Co., Japan). The viability of normal human oral keratocytes, gingival fibroblast, and gingival fibroblast immortalized by Human Papilloma virus 16 was measured in vitro for evaluation of cytotoxicity on resin cements, and the response of pulp tissue was analyzed and evaluated with light microscope after application of cements at cutting edge of incisors. The followings were drawn from this study : 1. The normal human oral keratocytes was the most sensitive to toxicity of resin cement, and toxicity of cements was higher in Bistite Ⅱ than in Variolink Ⅱ. 2. The cell viability of immortalized gingival fibroblast did not affected by type of cement and cultivation period, but there was a tendency that cytotoxicity in Bistite Ⅱ was higher than in Variolink Ⅱ. 3. The cell viability of gingival fibroblast was similar to that of immortalized gingival fibroblast regardless of cement type, but Bistite Ⅱ showed more toxic than others after 5 days cultivation 4. The responses of pulp tissue according to cement type were similar after 2 days cultivation, but revealed high toxicity in Bistite Ⅱ after 10 days cultivation.

턴디슈용 SiC 코팅材의 특성에 관한 硏究

김행식,최태현,이용구,전병세 경남대학교 신소재연구소 1995 論文集 Vol.5 No.-

턴디쉬 내장용으로 사용할 내화재료의 여러 가지 특성들을 시험하였다. 납석 70%, 점토 10% 그리고 탄화규소 20%을 혼합하여 18 가지의 조성을 가지는 시험편을 제조하였다. 탄화규소는 각각 순도 85%와 65%인 유럽산, 일본산, 중국산을 택하여 최종 제품에 미치는 영향을 알아보았다. 여러 가지 시험의 결과 10% 탄화규소를 포함한 시험편의 경우 점토질 내화벽돌 보다 수명이 2배 정도 향상되었으며 차지당 용손 두께도 1.5∼1.7㎜/ch였다. Various properties of refractory materials for the tundish lining were examined. The refractory samples with 18 different composition were prepared by mixing powders of 70 wt.% pyrophyllite. 10 wt.% clay and 20 wt.% Sic. SiC was selected from three sources, European. Japanese and Chinese-made, having 85% and 65% purities, to examine their effects on the final properties. As a results of several testings. the sample with 10% SiC(European1 was best suitable for tundish lining as a refractory coating materials. The wear depth was 1.2-1.5 mm per charge at the wall and 1.7 mm per charge a t the slag line, having shown that this service life was about 2 times longer than the ordinary lining made of clay firebricks(SiO₂-Al₂O₃).

침전성인자를 이용한 생물학적 고형물의 침전속도식과 고형물 플럭스 분석

김진우,최영균,이종훈,김희준,정태학 대한상하수도학회 2002 상하수도학회지 Vol.16 No.3

Equations on settling velocity of the activated sludge were developed by including sludge settleability parameters (SSP) and solids flux at a secondary settling tank was analyzed using the empirical equations, and an optimal solids loading rate was evaluated in this study. SVI, SSVI, DSVI and SSVI_3.5 were adopted as the SSP and a best fitting between observed and calculated zone settling velocity was obtained when SSVI_3.5 was used. Measured solids flux at the secondary settling tank was lower than that recommended by the national design criteria. Optimal solids loading rate and subsequent limiting solids flux can be derived if SSVI_3.5 is used instead of other SSP. Limiting solids flux calculated using SVI showed higher value than that calculated using SSVI_3.5 and the limiting solids flux could be better estimated when SSVI_3.5 was used because the calculated zone settling velocity using SSVI_3.5 was closer to the measured value.

Cadmium Chloride가 Mouse 의 췌장도 β-세포에 미치는 영향

전영희,백태경,정호삼,이규식,김영호 한양대학교 의과대학 1993 한양의대 학술지 Vol.13 No.1

As the industrial use of cadmium has become more common, the question of its toxicity has become more important. The present was undertaken to study effects of cadmium on the structure of β-cells in the Langerhans islet of the mouse pancreas. Healthy mice of ICR strain weighing around 39gm were used in this experiment. The each animal of the experimental group was interperitoneally administrated cadmium chloride 5.0mg/kg diluted in the water for injection. The experimental animals were sacrified at 3, 6, 12, 24, 48 and 72 hours after administration of cadmium chloride and pancreatic tissues was excised. Some specimens were stained with Halmi stain to observe the morphological changes in the endocrine portion of the pancreas, the islet of Langerhan, and the others were prefixed in the 2.5% glutaraldehyde-2% paraformaldehyde phosphate buffer solution (pH7.0) and postfixed in the 1% osmic acid. Ultrathin sections were made and double strained with uranyl acetate and lead citrate. These preparations were observed with light microscope and electron microscope as well. The results obtained were as follow; 1. The number of β-cells throughout the pancreatic islets were markedly reduced in the 12 hours group and the β-cells were increased slowly in the 24 hours group. 2. The cristae of mitochondria disappeared and electron-density of matrix in mitochondria is decreased in the 3 hours after treatment of animals with cadmium chloride. The mitochomdria appeared almost like normal group, in the 12 hours group. 3. The cisternae of rough endoplasmic reticulum were dilatated in the 3hours group and change in rough endoplasmic reticulum were completely restored to normal in the 24 hours group. 4. The Golgi complexes were very hypertophied in the 3 hours. The Golgi complexes were observed like normal group, in the 12 hours group. 5. The sectetory granules were hypertrophied a halo and loss a dense core and the electron dense core and the electron dense of core in secretory granules decreased in the 3 hours, 12 hours group. But the secretory granules were slightly restored in the 48 hours group and appeared almost like normal group in the 72 hours group. Consequently, it is concluded that cadmium chloride exerts several toxic effects to β-cells of Langerhan's islet, especially, the damage to secretory granule in mouse.

李光洙의 첫번째 유학 시대와 그 저작들

김태준 동국대학교 한국문학연구소 1992 한국문학연구 Vol.0 No.15

무기고분자응집제를 이용한 조류의 응집제거

김규동,최영균,김희준,곽종운,정태학 대한상하수도학회 2002 상하수도학회지 Vol.16 No.5

An Experimental study was conducted for the removal of algae using various inorganic coagulants. Cultivation of algae, investigation of turbidity and chlorophyll-a removal efficiency according to the dosage of coagulants were conducted in series using a jar-tester in this study. Alum, PAC (Polyaluminum chloride), PAC-2 (Concentrated Polyaluminum chloride), PAC-Ca (PAC with Ca), PFC (Polyferricchloride) and PACS (Polyaluminum chlorinate silicate) with various SiO_2 content were used as coagulants. Algae grew up to about 1,500㎍ chlorophyll-a/l in 30 days. Cultivated algae was diluted to 150㎍ chlorophyll-a/l for the laboratory experiment. Decrease of pH was the lowest when PACS-5 was used as a coagulant, while it was the highest when PAC was used. Host of the coagulants showed high turbidity removal rate when the dosage was 1-3 mg Al/l. PFC showed stable turbidity removal efficiency and 80% of removal efficiency could be obtained when the dosage was 1.34 mg Fe/l. Among the coagulants, PACSs showed relatively higher removal efficiency of chlorophyll-a and it was the highest when PACS-5 was used. It means that inorganic coagulant including silicate is more advantageous in the removal of algae, and appropriate content of silicate on the basis of molar ratio of Al to Si is an important factor affecting the stability and settleability of the alum floc.

摘出家兎肺의 理學的 性質에 關한 硏究

兪昌濬,金大洙 慶北大學校 1962 論文集 Vol.6 No.-

The physical natures, especially volume-pressure curves of the excised rabbit lung have been studied in number of experiments to see the mode of changes produced by the temperature and the velocity of air injected into the lung. Also an analytical observation was made on the specificity of the curves, and the results obtained are summarized as follows. (1) Pressure elevation following the injected of certain amount of air into the lung is proportionate to the speed of the air injection. The intratracheal pressure after the ceasation of the air injection decreased exponentially with time and the pressure difference between initial and equilibrium pressure was proportionate to the speed injection and the total amount of air injected. (2) The equilibrium pressure was observed unchanged with the speed throughout the entire experiments, bearing no particular relation with speed of the air injection. (3) The volume-pressure curve at the time of the air injection showed a parabola, its compliance being in proportion to the volume. (4) The volume-pressure curve in hypothermia state did not differ much from that of room temperature. However, its compliance was generally sell than nomal. (5) When the lung was kept under the condition of cooling and expansion for prolonged period of time, say over 12 hours, the volume-pressure curve of the equilibrium pressure showed nearly straight line, indicating increase of compliance and decrease of hysteresis. (6) The volume-pressure curve when the lung volume was diminished following the expansion, showed a parabola, its compliance being decreased at the beginning and increased at the end of the shrinking, A similar pattern was obtanined under different experimental conditions. (7) The volume-pressure curve, when the air was alternately injected into and ejected from the exciced lung showed a closed loop curve. Such a phenomenon of hysteresis could be explainde by the hypothetical basis that the lung is composed of the groups of alveoli having the different critical opening pressure.

DNA 수준에서의 형별법 확립이 시급한 HLA-Cw

안수진,전태연,김태규,한훈 대한조혈모세포이식학회 1999 대한조혈모세포이식학회지 Vol.4 No.1

조혈모세포 이식시 HLA 대립유전자 형벌이 갖는 중요성은 주지의 사실이고, 최근에는 타인간 조혈모세포이식이 활발히 진행되고 있어 더욱 정확한 HLA 대립유전자 형벌이 요구되고 있는 실정이다. 특히 HLA-Cw 항원은 자연살해세포와 상호 작용할 뿐만 아니라, HLA -A,B 항원과 마찬가지로 항원성 펩타이드를 세포용해성 T 림프구에 제시하여 면역 반응에 관여하고, 한가지 항원만이 검출되는 비율이 약50%나 되므로, HLA-Cw 대립유전자 형별은 필수적이라 하겠다. 그러나 HLA-Cw 항원은 HLA-A,B 항원과 비교하여 10%정도가 세포 표면에 표현되므로, 동종항체 형성률이 낮아 35종류 이상의 대립유전자가 존재하여도, 8종류 (Cw1-8) 의 표현형에 대한 항체만이 사용되고 있는 실정이다. 본 연구에 의하면 한국인에 존재하나 혈청학적인 방법으로 검출할 수 없었던 대립유전자가 약 20%에 달하고, 50%이상의 출현빈도를 보이는 Cw3 대립 유전자는 서로 다른 아형을 갖고 있다. 다시 말해, 현재의 HLA-Cw 대립유전자 형별법은 위음성률이 높고 부정확하므로 면역세포인 조혈모세포의 이식성공률을 놓이기 위해서는 DNA 수준에서의 형별법이 시급히 요청된다. In hemopoietic stem cell transplantation(HPSCT), the identification of HLA allele is very important. Recently, the unrelated hemopoietic stem cell transplantation (UHPSCT) is briskly progressed, and more accurate identification of HLA allele is required. By means of conventional serology, HLA-Cw cannot be phenotyped precisely. And in approximately 50% of the case, only one HLA-Cw molecule can be determined, so the accurate identification is very indispensible in HLA-Cw alleles. Especially, HLA-Cw molecules are interacted with class I receptors expressed on natural kill cells. Like HLA-A and -B, HLA-Cw molecules can present the fragmented antigens to cytotoxic T lymphocytes, which can be participated in immune response Therefore, the typing of HLA-Cw allele is indispensable in immune response. But HLA-Cw molecules are expressed to a low molecules (10%) compared to HLA-A, -B antigens. Although over 35 types of HLA-Cw allele are present, only 8 types (Cw1-Cw8) of HLA-Cw antibody have been used because evaluated rates of alloantibody are low. In this study, with the ARMs-PCR method, we identify 18 HLA-Cw alleles and over 20% of HLA-Cw alleles have not been identified by serology in normal Koreans (n=241). The most frequent HLA-Cw allele is HLA-Cw*03 which consists of different subtypes. And among them two subtypes, Cw*0303 and Cw*0304, comprise more than 50%. In conclusion, the conventional serology method may not detect an additional allele or may determine the allele incorrectly. It is suggested that DNA-based method is an urgently required step to increase the success rate of hemopoietic stem cell transplantation.

경식도 심초음파도의 Direct Planimetry를 이용하여 측정한 대동맥판 면적의 심박출량 및 좌심실 구출율의 변화에 따른 정확도

진승원,김종진,김희열,박지원,전두수,윤호중,박준철,노태호,채장성,김재형,홍순조,최규보 대한순환기학회 2000 Korean Circulation Journal Vol.30 No.8