Ginsenoside Rk1 ameliorates paracetamol-induced hepatotoxicity in mice through inhibition of inflammation, oxidative stress, nitrative stress and apoptosis

Jun-Nan Hu,Xing-Yue Xu,Wei Li,Yi-Ming Wang,Ying Liu,Zi Wang,Ying-Ping Wang 고려인삼학회 2019 Journal of Ginseng Research Vol.43 No.1

Background: Frequent overdose of paracetamol (APAP) has become the major cause of acute liver injury. The present study was designed to evaluate the potential protective effects of ginsenoside Rk1 on APAPinduced hepatotoxicity and investigate the underlying mechanisms for the first time. Methods: Micewere treated with Rk1 (10 mg/kg or 20 mg/kg) by oral gavage once per d for 7 d. On the 7th d, all mice treatedwith250mg/kgAPAPexhibitedsevere liver injuryafter24h, andhepatotoxicitywas assessed. Results: Our results showed that pretreatment with Rk1 significantly decreased the levels of serum alanine aminotransferase, aspartate aminotransferase, tumor necrosis factor, and interleukin-1b compared with the APAPgroup.Meanwhile, hepatic antioxidants, including superoxide dismutase andglutathione,were elevated compared with the APAP group. In contrast, a significant decrease in levels of the lipid peroxidation product malondialdehyde was observed in the ginsenoside Rk1-treated group compared with the APAP group. These effectswere associatedwith a significant increase of cytochromeP450 E1 and 4-hydroxynonenal levels in liver tissues. Moreover, ginsenoside Rk1 supplementation suppressed activation of apoptotic pathways by increasing Bcl-2 and decreasing Bax protein expression levels, which was shown using western blotting analysis.Histopathological observation also revealed that ginsenoside Rk1 pretreatment significantly reversed APAP-induced necrosis and inflammatory infiltration in liver tissues. Biological indicators of nitrative stress, such as 3-nitrotyrosine, were also inhibited after pretreatment with Rk1 compared with the APAP group. Conclusion: The results clearly suggest that the underlying molecular mechanisms in the hepatoprotection of ginsenoside Rk1 in APAP-induced hepatotoxicity may be due to its antioxidation, antiapoptosis, anti-inflammation, and antinitrative effects.

Ginsenoside Rk1 ameliorates paracetamol-induced hepatotoxicity in mice through inhibition of inflammation, oxidative stress, nitrative stress and apoptosis

Hu, Jun-Nan,Xu, Xing-Yue,Li, Wei,Wang, Yi-Ming,Liu, Ying,Wang, Zi,Wang, Ying-Ping The Korean Society of Ginseng 2019 Journal of Ginseng Research Vol.43 No.1

Background: Frequent overdose of paracetamol (APAP) has become the major cause of acute liver injury. The present study was designed to evaluate the potential protective effects of ginsenoside Rk1 on APAP-induced hepatotoxicity and investigate the underlying mechanisms for the first time. Methods: Mice were treated with Rk1 (10 mg/kg or 20 mg/kg) by oral gavage once per d for 7 d. On the 7th d, allmice treated with 250mg/kg APAP exhibited severeliverinjury after 24 h, and hepatotoxicitywas assessed. Results: Our results showed that pretreatment with Rk1 significantly decreased the levels of serum alanine aminotransferase, aspartate aminotransferase, tumor necrosis factor, and interleukin-$1{\beta}$ compared with the APAP group. Meanwhile, hepatic antioxidants, including superoxide dismutase and glutathione, were elevated compared with the APAP group. In contrast, a significant decrease in levels of the lipid peroxidation product malondialdehyde was observed in the ginsenoside Rk1-treated group compared with the APAP group. These effects were associated with a significant increase of cytochrome P450 E1 and 4-hydroxynonenal levels in liver tissues. Moreover, ginsenoside Rk1 supplementation suppressed activation of apoptotic pathways by increasing Bcl-2 and decreasing Bax protein expression levels, which was shown using western blotting analysis. Histopathological observation also revealed that ginsenoside Rk1 pretreatment significantly reversed APAP-induced necrosis and inflammatory infiltration in liver tissues. Biological indicators of nitrative stress, such as 3-nitrotyrosine, were also inhibited after pretreatment with Rk1 compared with the APAP group. Conclusion: The results clearly suggest that the underlying molecular mechanisms in the hepatoprotection of ginsenoside Rk1 in APAP-induced hepatotoxicity may be due to its antioxidation, antiapoptosis, anti-inflammation, and antinitrative effects.

The intrinsic relationship between color variation and performances of the deteriorated aviation lubrication oil

Jun Ma,Shaojun Ruan,Jianqiang Hu,Yuanbao Sun,Yiwei Fei,Xufeng Jiang,Shibao Dong,Teng Chen,Nan Wu 한국공업화학회 2020 Journal of Industrial and Engineering Chemistry Vol.92 No.-

Dose the blackened lubrication oil needs to be replaced forflying safety? Since there is very limited roomfor inaccurate assessment of oil quality due to strictflying safety standards, much aviation lubrication oilis disposed in advance long before their service life. More important, aircrafts still face the fatalchallenges from unpredictable lubrication oil deterioration. Color change is surely a significant signal to diagnose the quality of lubrication oils. Based on thecolorimeter, the color numbers of the testing lubrication oils are measured in this paper to identify thecolor variation degree of the oxidized lubrication oils. Then the molecular structures of the oxidizedlubrication oils are investigated tofind the intrinsic relationship between the oxidation products andcolor numbers. Based on key performances of the oxidized oil samples, the oil quality is undoubtfullydeteriorated which is coincident with the variation of color number. The color numbers of the usedaviation lubrication oils could provide an alternative way to readily and quickly measure the declinedegree and residual lifetime.

Phenolic composition and nutritional attributes of diaphragma juglandis fructus and shell of walnut (Juglans regia L.)

Qiang Hu,Jia Liu,Jun Li,Hui Liu,Nan Dong,Yang-yang Geng,Yang Lu,Yan Wang 한국식품과학회 2020 Food Science and Biotechnology Vol.29 No.2

Phenolic composition and nutritional attributes of diaphragma juglandis fructus (Djf) and walnut shells (Ws) were investigated. Phenolic acids, hydroxybenzoic acid, isoflavone, and flavone were identified in the free phenolic fractions (FPFs) of both Djf and Ws. Bound phenolic fractions were less than FPFs both in content and diversity. The soluble dietary fiber contents of Djf and Ws were 25.56 g/100 g and 9.5 g/100 g, respectively. The contents of unsaturated fatty acids (1912.28 mg/kg and 9137.56 mg/kg, respectively) were significantly higher than that of saturated fatty acid both in Djf and Ws. The content of essential amino acids in Djf (9.67 mg/g) was significantly higher than that in Ws. More than eight types of monosaccharides were detected in Djf and Ws. The monosaccharide content of Djf (314.16 mg/g) was significantly higher than that of Ws (60.97 mg/g). Trehalose was the predominant component both in Djf (71.2%) and Ws (78.6%).

Roles of Fibroblast Growth Factor-inducible 14 in Hepatocellular Carcinoma

Li, Nan,Hu, Wen-Jun,Shi, Jie,Xue, Jie,Guo, Wei-Xing,Zhang, Yang,Guan, Dong-Xian,Liu, Shu-Peng,Cheng, Yu-Qiang,Wu, Meng-Chao,Xie, Dong,Liu, Shan-Rong,Cheng, Shu-Qun Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.6

The prognostic value of the fibroblast growth factor-inducible 14 (Fn14) expression in hepatocellular carcinoma (HCC) is unknown. Real-time PCR (RT-PCR), western blot assays and immunohistochemistry analysis were here performed in order to compare Fn14 expressios in paired liver samples of HCC and normal liver tissue. Most of the tumor tissues expressed significantly higher levels of Fn14 compared to adjacent non-tumor tissues, with Fn14High accounting for 54.6% (142/260) of all patients. The Pearson ${\chi}^2$ test indicated that Fn14 expression was closely associated with serum alpha fetal protein (AFP) (P=0.002) and tumor number (p=0.019). Univariate and multivariate analyses revealed that along with tumor diameter and portal vein tumor thrombosis (PVTT ) type, Fn14 was an independent prognostic factor for both overall survival (OS) (HR=1.398, p=0.008) and recurrence (HR=1.541, p=0.001) rates. Fn14 overexpression HCC correlated with poor surgical outcome, and this molecule may be a candidate biomarker for prognosis as well as a target for therapy.

라포가 고객 행동에 미치는 영향 : 친숙성, 관여도의 조절효과를 중심으로

전성준(Quan, Cheng Jun),강기두(Kang, Gi Du),장은서(Jang, Eun Seo),박호남(Piao, Hu Nan) 글로벌경영학회 2021 글로벌경영학회지 Vol.18 No.1

본 연구는 중국에 있는 하이디라오(海底捞) 훠궈 레스토랑을 방문경험이 있는 사람들을 대상으로 종업원과 고객 간의 라포가 고객행동에 미치는 영향 및 라포에 대한 동일시와 신뢰를 통해 고객들의 행동이 형성되어짐을 실증분석을 통해 검정하고자 하였다. 그리고 라포와 신뢰 및 동일시 간에 어떤 대상에 대해 인지도나 관심도에 따라 차이가 있을 것이라고 예상하며 친숙성, 관여도라는 조절변수를 사용하였다. 총 455부의 설문지를 배포하여 최종분석에 431명의 설문 자료를 SPSS 23.0과 AMOS를 이용하여 분석하였다. 실증분석 결과는 다음과 같다. 첫째, 라포는 동일시와 신뢰에 긍정적인 영향을 미치는 것으로 나타났고, 이어 동일시와 신뢰는 고객참여행동과 고객시민행동에 긍정적인 영향을 미치는 것으로 나타났다. 반면 라포와 고객행동간의 직접적인 관계를 살펴본 결과, 라포는 고객시민행동에는 유의한 영향을 미치지만, 고객참여행동에 미치는 영향은 유의하지 않은 것으로 나타났다. 둘째, 라포와 고객행동간의 관계에서 동일시 및 신뢰의 매개효과를 살펴본 결과, 매개효과를 보여주는 간접 효과가 모두 통계적으로 유의하였다. 마지막으로 친숙성과 관여도의 정도에 따라 라포가 동일시와 신뢰에 미치는 영향이 어떻게 달라지는지를 살펴보았는데 라포와 동일시, 라포와 신뢰의 관계 에서 친숙성 및 관여도에 따른 조절효과가 있는 것으로 나타났다. 본 연구의 검증결과를 토대로 볼 때, 고객이 레스토랑 서비스나 상품을 사용하는 과정에서 종사 원과 즐거운 관계를 유지할 수 있도록 고객의 동일시, 고객신뢰를 파악함을 통해 고객의 자발적 행동을 적극적으로 유도할 수 있다. Rapport is a pleasant interaction between employees and customers; when it is well formed, it can have a positive effect on the customer s behavior and attitude. To this end, companies work hard to improve the satisfactory relationship between employees and customers since it could increase the bond between them. The current study attempted to address the role of rapport in relation with other constructs upon to the literature reviews. Empirical study investing on the customers of restaurant in China and the results are summarized as follows: firstly, rapport exerts a positive correlation on the customer behavior; secondly, identification and trust play a mediating role on the relationship between rapport and customer behavior. Thirdly, familiarity and involvement has the moderating effect on the rapport with identification and trust. Based on such research results, several academic and managerial implications were proposed and at the same time, the limitations for the study were also raised.

Genetic Variation and Evolution of the Pi9 Blast Resistance Locus in the AA Genome Oryza Species

Jinling Liu,Yajun Hu,Yuese Ning,Nan Jiang,Jun Wu,전종성,Yinghui Xiao,Xionglun Liu,Liangying Dai,Guo-Liang Wang 한국식물학회 2011 Journal of Plant Biology Vol.54 No.5

The rice nucleotide-binding site–leucine-rich repeat (NBS-LRR)-encoding resistance (R) gene Pi9 confers broad-spectrum resistance to the fungal pathogen Magnaporthe oryzae. The Pi9 locus comprises many NBS-LRR-like genes and is an ancient locus that is highly conserved in cultivated and wild rice species. To understand the genetic variation and molecular evolutionary mechanism of the Pi9 alleles in different rice species, we studied five AA genome Oryza species including two cultivated rice species (Oryza sativa and Oryza glaberrima) and three wild rice species (Oryza nivara, Oryza rufipogon, and Oryza barthii). A 2.9-kb fragment spanning the NBS-LRR core region of the Pi9 gene was amplified and sequenced from 40 accessions. Sequence comparison revealed that the Pi9 alleles had an intermediate-diversified nucleotide polymorphism among the AA genome Oryza species. Sequence variations were more abundant in the LRR region than in the NBS region, indicating that the LRR region has played a more important role for the evolution of the Pi9alleles. Furthermore, positive selection was found to be the main force promoting the divergence of the Pi9 alleles,especially in the LRR region. Our results reveal the characteristics and evolutionary dynamics of the Pi9 alleles among the two cultivated and three wild rice species.

High-Level Expression of an Aspergillus niger Endo-β-1,4-Glucanase in Pichia Pastoris Through Gene Codon Optimization and Synthesis

( Shu Miao Zhao ),( Jun Huang ),( Chang Yi Zhang ),( Ling Deng ),( Nan Hu ),( Yun Xiang Liang ) 한국미생물 · 생명공학회 2010 Journal of microbiology and biotechnology Vol.20 No.3

Tumor-Derived Transforming Growth Factor-β is Critical for Tumor Progression and Evasion from Immune Surveillance

Li, Zheng,Zhang, Li-Juan,Zhang, Hong-Ru,Tian, Gao-Fei,Tian, Jun,Mao, Xiao-Li,Jia, Zheng-Hu,Meng, Zi-Yu,Zhao, Li-Qing,Yin, Zhi-Nan,Wu, Zhen-Zhou Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.13

Tumors have evolved numerous mechanisms by which they can escape from immune surveillance. One of these is to produce immunosuppressive cytokines. Transforming growth factor-${\beta}$(TGF-${\beta}$) is a pleiotropic cytokine with a crucial function in mediating immune suppression, especially in the tumor microenvironment. TGF-${\beta}$ produced by T cells has been demonstrated as an important factor for suppressing antitumor immune responses, but the role of tumor-derived TGF-${\beta}$ in this process is poorly understood. In this study, we demonstrated that knockdown of tumor-derived TGF-${\beta}$ using shRNA resulted in dramatically reduced tumor size, slowing tumor formation, prolonging survival rate of tumor-bearing mice and inhibiting metastasis. We revealed possible underlying mechanisms as reducing the number of myeloid-derived suppressor cells (MDSC) and $CD4^+Foxp3^+$ Treg cells, and consequently enhanced IFN-${\gamma}$ production by CTLs. Knockdown of tumor-derived TGF-${\beta}$ also significantly reduced the conversion of na$\ddot{i}$ve $CD4^+$ T cells into Treg cells in vitro. Finally, we found that knockdown of TGF-${\beta}$ suppressed cell migration, but did not change the proliferation and apoptosis of tumor cells in vitro. In summary, our study provided evidence that tumor-derived TGF-${\beta}$ is a critical factor for tumor progression and evasion of immune surveillance, and blocking tumor-derived TGF-${\beta}$ may serve as a potential therapeutic approach for cancer.

Ginsenoside Rh2 attenuates microglial activation against toxoplasmic encephalitis via TLR4/NF-kB signaling pathway

Xiang Xu,Lan Jin,Tong Jiang,Ying Lu,Fumie Aosai,Hu-Nan Piao,Guang-Hua Xu,Cheng-Hua Jin,Xue-Jun Jin,Juan Ma,Lian-Xun Piao 고려인삼학회 2020 Journal of Ginseng Research Vol.44 No.5

Background: Ginsenoside Rh2 (GRh2) is a characterized component in red ginseng widely used in Korea and China. GRh2 exhibits a wide range of pharmacological activities, such as anti-inflammatory, antioxidant, and anticancer properties. However, its effects on Toxoplasma gondii (T. gondii) infection have not been clarified yet. Methods: The effect of GRh2 against T. gondii was assessed under in vitro and in vivo experiments. The BV2 cells were infected with tachyzoites of T. gondii RH strain, and the effects of GRh2 were evaluated by MTT assay, morphological observations, immunofluorescence staining, a trypan blue exclusion assay, reverse transcription PCR, and Western blot analyses. The in vivo experiment was conducted with BALB/c mice inoculated with lethal amounts of tachyzoites with or without GRh2 treatment. Results and conclusion: The GRh2 treatment significantly inhibited the proliferation of T. gondii under in vitro and in vivo studies. Furthermore, GRh2 blocked the activation of microglia and specifically decreased the release of inflammatory mediators in response to T. gondii infection through TLR4/NF-kB signaling pathway. In mice, GRh2 conferred modest protection from a lethal dose of T. gondii. After the treatment, the proliferation of tachyzoites in the peritoneal cavity of infected mice markedly decreased. Moreover, GRh2 also significantly decreased the T. gondii burden in mouse brain tissues. These findings indicate that GRh2 exhibits an antieT. gondii effect and inhibits the microglial activation through TLR4/NF-kB signaling pathway, providing the basic pharmacological basis for the development of new drugs to treat toxoplasmic encephalitis.