Influence of Microstructure and Mechanical Properties on Formability in High Strain Rate Rolled AZ31 Magnesium Alloy Sheets

Fubao Liu,Xiao Liu,Biwu Zhu,Hui Yang,Gang Xiao,Mingyue Hu 대한금속·재료학회 2022 METALS AND MATERIALS International Vol.28 No.6

High strain-rate rolling (≥ 10 s−1) has been successfully employed to produce AZ31 magnesium alloy sheets to a high reductionof 82% with a fine grain structure in a single pass. The rolled sheets inevitably undertake various forming processessuch as press and deep forming in the fabrication stage. To investigate the formability, the Erichsen tests were carried out onhigh strain rate rolled AZ31 magnesium alloy sheets at room temperature. The relationship among mechanical properties,microstructure and formability were investigated. The crack behavior during Erichsen tests was also studied. It was foundthat the formability was related to the lowest in-plane tensile strengthen and dislocation density of initial microstructure. The competitive relationship between micro-crack and twinning/shear bands would also influence the formability of highstrain rate rolled magnesium alloy sheets. Deformed shear band with substaintial twins prohibits the crack extension andenhances strengthen of materials in front of crack tip.

"五四"何以失精神

劉慧軍 한국중국문화학회 2000 中國學論叢 Vol.9 No.-

5·4운동은 중국 역사상 위대한 사상변혁운동이다. 5·4운동이 제창한 "立人"사상은 당시뿐만 아니라 현재에 있어서도 중국문화의 발전과 변화에 중요한 의의를 지니고 있다. 그러나 여러 가지 원인 때문에 5·4정신은 잊혀졌다. 본고는 정치와 문화적 측면에서 5·4정신이 잊혀지게 된 원인을 검토한 바, 다음 3가지가 주된 원인으로 작용하였다고 분석하였다. 첫째, 중국전통문화의 뿌리가 확고하여 민주사상이 단시간 내에 확립될 수 없었다. 둘째, 정치의 막강한 흡인력이 신문화운동 발전에 영향을 미쳐 지식인이 문화적 사고를 포기하여, 중국의 사상계몽이 시작되자마자 중단되었다. 셋째, 5·4운동 후 권력자의 남용이 5·4정신을 잊혀지게 하는 중요한 원인이 되었다.

Array-based Nano-amplification Technique Was Applied in Detection of Hepatitis E Virus

( Hui Hui Liu ),( Xuan Cao ),( Yong Yang ),( Ming Gui Liu ),( Ye Fu Wang ) 생화학분자생물학회 2006 BMB Reports Vol.39 No.3

Array-based Nano-amplification Technique Was Applied in Detection of Hepatitis E Virus

Liu, Hui-Hui,Cao, Xuan,Yang, Yong,Liu, Ming-Gui,Wang, Ye-Fu Korean Society for Biochemistry and Molecular Biol 2006 Journal of biochemistry and molecular biology Vol.39 No.3

A rapid method for the detection of Hepatitis E Virus (HEV) was developed by utilizing nano-gold labeled oligonucleotide probes, silver stain enhancement and the microarray technique. The 5'-end -$NH_2$ modified oligonucleotide probes were immobilized on the surface of the chip base as the capture probe. The detection probe was made of the 3'-end -SH modified oligonucleotide probe and nano-gold colloid. The optimal concentrations of these two probes were determined. To test the detection sensitivity and specificity of this technique, a conservative fragment of the virus RNA was amplified by the RT-PCR/PCR one step amplification. The cDNA was hybridized with the capture probes and the detection probes on microarray. The detection signal was amplified by silver stain enhancement and could be identified by naked eyes. 100 fM of amplicon could be detected out on the microarray. As the results, preparation of nano-gold was improved and faster. Development time also was shortened to 2 min. Thus, considering high efficiency, low cost, good specificity and high sensitivity, this technique is alternative for the detection of HEV.

Mitochondrial citrate accumulation drives alveolar epithelial cell necroptosis in lipopolysaccharide-induced acute lung injury

Yang Hui-Hui,Jiang Hui-Ling,Tao Jia-Hao,Zhang Chen-Yu,Xiong Jian-Bing,Yang Jin-Tong,Liu Yu-Biao,Zhong Wen-Jing,Guan Xin-Xin,Duan Jia-Xi,Zhang Yan-Feng,Liu Shao-Kun,Jiang Jian-Xin,Zhou Yong,Guan Cha-Xi 생화학분자생물학회 2022 Experimental and molecular medicine Vol.54 No.-

Necroptosis is the major cause of death in alveolar epithelial cells (AECs) during acute lung injury (ALI). Here, we report a previously unrecognized mechanism for necroptosis. We found an accumulation of mitochondrial citrate (citratemt) in lipopolysaccharide (LPS)-treated AECs because of the downregulation of Idh3α and citrate carrier (CIC, also known as Slc25a1). shRNA- or inhibitor–mediated inhibition of Idh3α and Slc25a1 induced citratemt accumulation and necroptosis in vitro. Mice with AEC-specific Idh3α and Slc25a1 deficiency exhibited exacerbated lung injury and AEC necroptosis. Interestingly, the overexpression of Idh3α and Slc25a1 decreased citratemt levels and rescued AECs from necroptosis. Mechanistically, citratemt accumulation induced mitochondrial fission and excessive mitophagy in AECs. Furthermore, citratemt directly interacted with FUN14 domain-containing protein 1 (FUNDC1) and promoted the interaction of FUNDC1 with dynamin-related protein 1 (DRP1), leading to excessive mitophagy-mediated necroptosis and thereby initiating and promoting ALI. Importantly, necroptosis induced by citratemt accumulation was inhibited in FUNDC1-knockout AECs. We show that citratemt accumulation is a novel target for protection against ALI involving necroptosis.

De novo assembly of the transcriptome for Greenbug (Schizaphis graminum Rondani) and analysis on insecticide resistance-related genes

Jun-Jie LIU,Liu-Yang LU,Hui-Hui LIU,Ya-She LI,Xu SU,Bai-Zhong ZHANG,Xi-Ling CHEN 한국곤충학회 2019 Entomological Research Vol.49 No.8

The greenbug, Schizaphis graminum Rondani is a major pest species of wheat crops. In this study, a transcriptome sequencing, and the expression of the 12 genes related to insecticide resistance were conducted in S. graminum. The sequencing and subsequent bioinformatics analysis outputed 46,593 unigenes, among which 28,289 unigenes were annotated to corresponding functions by blasting with high homologous genes in database, giving annotation rate of 60.72%. To gain insight into the mechanism of insecticide resistance, the expression of the 12 genes related to insecticide resistance for S. graminum was investigated. The expression level of aminopeptidase N (AN), cytochrome P450 (CYP), acetylcholinesterase 1 (AC), catalase (CAT), cytochrome c oxidas (CCC), GABA receptor (GABA), glutathione S-transferase (GST) were highest in the apterous nymphs among different developmental stages; The expression level of AN, CBL, CYP, CA, SD, and GST were relatively more abundant in the abdomen compared to head and throax. The results could give out the key information about the relationship between the expression of these genes in different developmental stages, tissues, treatments and metabolism of insecticides. These genes that were co-up-regulated significantly in third instar nymphs of S. graminum induced by imidacloprid, were consistent with their putative involvement in insecticide resistance. This provides most comprehensive transcriptome data for S. graminum to further study and managenment.

Genome-Wide Analysis of the Thioredoxin Gene Family in Gossypium hirsutum L. and the Role of the Atypical Thioredoxin Gene GhTRXL3-2 in Flowering

Liu Hui,Li Yunfei,Huang Xianzhong 한국식물학회 2021 Journal of Plant Biology Vol.64 No.5

Thioredoxin (TRX) is a highly conserved low-molecular-weight protein and a ubiquitous antioxidant enzyme that plays key role in the regulation of plant growth and development. Here, using the whole-genome sequence, we performed a systematic analysis for the TRX gene family in upland cotton (Gossypium hirsutum L.) and analyzed their structural characteristics, evolution, and expression profiles during growth and development. At least 86 GhTRX members, 40 typical and 46 atypical, were identified in the cotton genome, and they were unevenly distributed on the 26 chromosomes. Conserved domains and phylogenic tree construction classified the typical TRX gene family into seven subfamilies and the atypical TRX into nine subfamilies. An evolutionary analysis revealed that the TRX gene family underwent purification selection during evolution. In addition, an RNA-Seq analysis showed that, during vegetative and reproductive development, the differences in transcript abundance levels and organ-specific expression patterns suggest functional diversity. Biochemical assays demonstrated that the atypical TRX protein GhTRXL3-2 interacted with the cotton FLOWERING LOCUS T protein GhFT. The overexpression of GhTRXL3-2 in Arabidopsis thaliana resulted in early flowering compared with control plants. Additionally, the silencing of GhTRXL3-2 in cotton delayed maturation, suggesting that it has important roles in cotton’s flowering regulation. These results help clarify the evolution of the TRX genes and elucidate their biological functions in cotton flowering regulation.

Influence of Soil Erosion Thickness on Soybean Yield and Coupling Mode of Water and Fertilizers of Black Soil in Northeast China

Hui Liu,Yongxia Wei,Long Wang,Xian Wu 보안공학연구지원센터 2015 International Journal of u- and e- Service, Scienc Vol.8 No.3

Soil erosion is one of the most widespread ecological problems with the most serious damage worldwide. In the present study, the influence of soil erosion thickness on soybean yield of black soil in Northeast China is studied. With the pot experiment as the method, this study investigates the influence by peeling the surface black soil layer at 0, 5, 10, 20, 25, and 30 cm to simulate different soil erosion thicknesses, establishes a quantitative model of soybean yield to explore the response of soybean yield to soil erosion thickness. The 4-D-optimum orthogonal design (416-A) is adopted to conduct the coupling experiment of water and fertilizers under erosion thicknesses of 0 and 15 cm so that the coupling modes of water and fertilizers under the two soil erosion thicknesses are established. The results show that soil erosion thickness has a significant influence on soybean growth, that is, with the increase in the soil erosion thickness, soybean plant height, pods and grains per plant all reduce, and soybean yield decreases in a Z-shaped curve, while yield reduction rate increases in a S-shaped curve, respectively. Water, along with N, P, and K fertilizers, affect soybean yield, but the intensity varies with soil erosion thickness. Under the soil erosion thickness of 0 cm, the order is: W>P>N>K. While under the soil erosion thickness of 15 cm, the order is: N>W>P>K. With the increase in soil erosion thickness and the decrease in soil fertility, the effect of irrigation reduces, whereas that of fertilization increases. The research results can provide the theoretical basis for soil erosion prevention and control in the black soil region.

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LIU HUI,이상근 한국경영정보학회 2023 한국경영정보학회 학술대회논문집 Vol.2023 No.06

Control Strategy for an Electromechanical Transmission Vehicle Based on a Double Markov Process

Liu Hui,Xu Riming,Han Lijin,Xiong Shanshan 한국자동차공학회 2021 International journal of automotive technology Vol.22 No.3

Based on an analysis of the driving demand and system dynamics of heavy-duty vehicles equipped with electromechanical transmission (EMT), a double Markov model is put forward to represent drivers’ power demand for driving and electricity. Transfer probability matrices are calculated by utilizing the maximum likelihood estimation method. A power distribution control strategy based on stochastic dynamic programming (SDP) is proposed. With economy being the optimization goal, the model for power allocation control based on SDP is established while regarding the engine torque, motor speeds, vehicle speed and state of charge (SOC) as state variables’ engine speed and motor torques as control variables’ and power demands as interference variables. The SDP problem is solved by an improved policy iteration algorithm based on value iteration and policy iteration algorithms.