Site-Directed Mutagenesis법에 의한 RecA 단백질의 ATPase 활성 부위 검정 : Cysteinyl 129의 관련성 Involvement of Cysteinyl 129

배준성,한태룡 경희대학교 유전공학연구소 1992 遺傳工學論文集 Vol.4 No.-

ATPase activity of RecA protein is believed to be essential for the strand exchange and DNA repair reactions in E. coli. Chemical modification(Kuramitsu et al., Biochemistry. 23. 2363. 1984) and ATP photolabeling(Banks and Sedgwick. Biochemistry, 25, 5882, 1986) experiments suggest that cysteinyl-129 is one of the most probable residues for the binding and hydrolysis of ATP in RecA protein. Site-directed mutagenesis is employed to change cysteinyl-129 to serine and alanine to minimize any structural alterations. Wild type and mutant RecA proteins were purified and examined for ATPase activity. All the purified RecA proteins showed sigmoidal activity shapes as a function of substrate concentration, indicating that RecA catalyzed ATP hydrolysis is cooperative. Both mutant RecAs displayed almost same V_(max) with wild type RecA but much larger S_(1/2) values(l80 μM for Ser. 120 μM for ,A1a) than wild type(78 μM). These results suggest that cysteinyl-129 may involve in the ATP binding rather than ATP hydrolysis. Further experiments on direct ATP binding and strand exchange reactions are in progress.

규조토로부터 β-Sialon의 합성

배원태,전병세,최태현,문종수 慶南大學校 附設 工業技術硏究所 1989 硏究論文集 Vol.7 No.-

카올린에 비하여 Al₂O₃의 함량이 낮은 규조토로부터 β-Sialon을 합성하였다. 불순물의 함량을 저하시키고, 반응을 증대시키기 위하여 규조토를 산처리하였다. 산처리하므로써 알카리 함량이 저하되고, 비표면적은 증가하며, 또한 Al₂O₃의 함량이 낮은 규조토를 사용한 β-Sialon에서는 미반응 Al₂O₃가 존재하지 않았다. β-Sialon was synthesized from diatomaceous earth which had the lower content of Al₂O₃ than kaolin. Diatomaceous earth was treated by acid in order to reduce impurity level and to increase reactivity. Contents of alkali were reduced and specific surface area was increased in acid treated samples. Free Al₂O₃ did not exist in the final samples which were synthesized from the low Al₂O₃ contented diatomaceous earth.

고속도강공구의 절삭온도 예측과 플랭크마모에 관한 연구

전태옥,전언찬,박흥식,배춘익 동아대학교 공과대학 부설 한국자원개발연구소 1995 硏究報告 Vol.19 No.2

Temperature distribution on the flank face in orthogonal turning with cutting tool of high speed steel is studied by using a finite element method and experiments. Experiments are carried out to verify the validity of the temperature measurement by using a thermoelectric couple junction imbedded in a cutting tool. Good agreement is obtained between the analytical results and the experimental ones for the temperature distributions on flank face of cutting tool Flank wear were observed by the tool microscope. The analytical results show that the temperature on the top flank face of a tool is higher because of the difference of the friction velocity on each face of the tool.

결핵균항원에 대한 폐결핵환자의 임파구 증식반응에 관한 연구

백태현,김준배,박정규,김화중,조은경,최대경 충남대학교부설 생명공학연구소 1992 생물공학연구지 Vol.2 No.-

T lymphocytes are thought to play a central role in cell mediated immune response. To study the T lymphocyte proliferative response to purified 30-kDa antigen from Mycobacterium tuberculosis H37Rv, peripheral blood lymphocytes(PBL) isolated from healthy controls and tuberculosis patients were stimulated with the 30-kDa antigen, crude antigen and PHA. Healthy controls and tuberculosis patients were divided into PPD(+), PPD(-) groups and AFB(-), AFB(+) groups, respectively. PBL proliferation were determined by the ^3H-thymidine incorporation assay and MTT colorimetric assay. PBL proliferation with 30-kDa and crude protein antigens measured by both methods were almost identical(r=0.74, p<0.001). The lymphocyte proliferation to 30-kDa antigen and crude antigen were singnificantly increased in PPD(+) healthy controls and tuberculosis patients when compared to those in PPD(-) controls, but response to PHA was no significant difference. Analysis to T cell subsets of proliferated lymphocytes performed by the indirect immunoalkaline phosphatase techniques were no difference between not only healthy controls and patients groups, but also stimulated antigen or PHA, and especially, at all groups, the percentage of T8 cell was higher than that of T4 cell.

응집에 의한 생물학적 처리수의 색도 및 난분해성 유기물 제거

서태경, 박상민, 박노백, 임지영, 전항배 충북대학교 건설기술연구소 2008 충북대학교 건설기술연구소 논문집 Vol.27 No.1

This study was to evaluate the optimum conditions for chemical coagulation to remove color and non-biodegradable organic matters from the biologically treated water of piggery wastewater. Raw water was fractionated by characteristics of dissolved organic matter. The fractions of hydrophilic substance and molecular weight cut-off(MWCO) below 500 in raw water were 51% and 74%, respectively. SC(streaming current) was measured to find out the optimum pH values and dosages for aluminium sulfate[Al₂(SO₄)₃·14H₂O] and ferric chloride[FeCl₃·6H₂O] as a coagulant, which was compared with color and turbidity removal efficiencies followed by coagulation, flocculation and sedimentation. From several jar tests, optimum pH values and dosages of coagulants were well matched with the results of SCD analyses. The optimum dosages(pH values) of aluminium and ferric were 5.84 mM(pH 4.0) and 7.03 mM(pH 5.6), respectively. At optimum conditions of coagulation, color removal efficiencies for aluminium sulfate and ferric chloride were as high as 89.3 and 97.9%, respectively. Chemical coagulation showed good performance to remove color and non-biodegradable organic matters from biologically treated water.

포괄수가지불제도 적용에 따른 산부인과 진료수입의 변화

황태연,유병철,정귀언,정수진,김성준,배기택,손혜숙,이종태,전진호,엄상화 인제대학교 2001 仁濟醫學 Vol.22 No.1

Objectives: Under the fee-for-service system, Korean hospitals have been suffering from the worsening profitability caused by law medical care fees. To overcome the difficulties, they have maximized the quantity of medical services and made relatively large investment in developing new medical services using high-priced medical equipment, medicines and medical supplies which were not covered by medical insurance system rather than reduced running costs. Materials and Methods: This study was made to analyze and forecast the revenue change of the department of Obstetrics and gynecology of an university hospital in Seoul, expecting the implementation of DRG system in 2000. Results: The results were as follows : 1.During the first two days of hospitalization. 41% of total medical service fee was occurred, which was thought that most of the check-ups and operations were made during this period. After two days of hospitalization, the new occurrence of medical service fee tended to reduce. 2.Out of total medical service fee, 67% of admission fee was occurred after 5 days of hospitalization. This was because medical service fees in obstetrics and gynecology department occurred during the first 5 days of hospitalization. 3.Out of fees for operation, treatment, medical supplies and blood transfusion. 71% was occurred during the first two days. 4. In case of fees for examination, 50% was occurred during the first two days. 5.A total of 53% of fees for medication and injection was occurred during the first 5 days. Conclusions: By the implementation of the DRG system, the income is forecast to increase by 800 won to 310,000 won by the disease group of obstetrics and gynecology department. To increase hospital income with the implementation of DRG system, the results of this study suggest that examinations should be done at out-patient departments before the hospitalization of patient, the discharge of patient should be noticed in advance to reduce the period of hospitalization, and admission appointment system should be implemented for the immediate operation and delivery.

급성 말초 동맥 폐색증의 치료

박형준,박연고,고영환,서태석,이운기,박홍규,백정흠,이정남,정민,이영돈,민승기 대한혈관외과학회 2003 Vascular Specialist International Vol.19 No.1

레이저 경화면의 형상특징 해석을 위한 프랙탈 차원의 적용

옥철호,조연상,배춘익,박흥식,전태옥 동아대학교 생산기술연구소 2000 生産技術硏究所硏究論文集 Vol.5 No.2

The determination of surface topography is believed to be extremely important in the areas of contact mechanics, adhesion and friction. In order to describe topography of various frictional surface, the harding test was carried out under different experimental conditions in dry friction. And fractal descriptors was applied to frictional surface of laser modified steel with image processing system. These descriptors to analyze surface structure are fractal dimension. Surface fractal dimension can be determined by sum of intensity difference of surface pixel. Topography of frictional surface can be effectively obtained by fractal dimensions.

충청지역 사육 돼지의 호흡기 및 소화기 병변으로부터 분리된 병원세균의 약제 감수성

김지영,김태용,장경수,김석,허지연,박배근,전무형 충남대학교 수의과대학 동물의과학연구소 2000 動物醫科學硏究誌 Vol.8 No.-

During the past two years(1999-2000), the antimicrobial susceptibility tests for the major pathogenic bacteria isolated from the respiratory or digestive organs of the diseased pigs in Chungchong area have been carried out. Total of 88 strains in seven species were isolated from the respiratory lesions of the diseased pigs during the periods, and the major isolates were in order of P. multocida(27.3%), Staphylococcus spp.(20.4%), Streptococcus spp.(15.9%), E. coli(12.5%), B. bronchiseptica(11.4%), Haemophilus spp.(10.2%) and A. pleuroneumoniae(2.3%). From the fecal specimens of the pigs with diarrhoea, total of 146 strains in five species were isolated in order of E. coli(59.6%), Salmonella spp.(17.8%), Campylobacter spp.(14.4%), Streptococcus spp.(5.5%) and Clostridium spp.(2.7%). The isolates were tested the susceptibility for 30 kinds of antibiotics. Among the isolates from the swine repiratory lesions, P. multocida revealed over 71% of susceptibility to ciprofloxacin(CIP), enrofloxacin(EF), tylosine(TL) and norfloxacin(NOR). B. bronchiseptica showed over 70% susceptibility to CIP, EF, linco-spectin(LS) and NOR, and Haemophilus spp. over 78% of susceptibility to AN, CIP, EF and NOR. The isolates of Streptococcus spp. revealed over 80% susceptibility to CIP and EF, and Staphylococcus spp. over 78% of high susceptibility to amikacin(AN), cephalosporin(CP) and CIP. .In general, most of isolates were highly resistant to ampicillin(AM), bacitracin(B), ceftiofer(CT), chlorampenicol(C), colistin(CL), erythromycin(E), nalidixic acid(NA), novobiocin(NB), oxacillin(OX), penicillin G(P), polymyxin B(PB) and vancomycin(VA). Of the isolates from the pigs with diarrhoea, over 76% of E. coli isolates showed the high susceptibility to AN, LS and N. Salmonella spp. revealed over 71% of susceptibility to CT, CIP, EF, LS and NOR, and Campylobacter spp., over 76% of susceptibility to CT ,CIP, EF, GM, LS and TL. Higher susceptibility were observed CIP, LS, NOR and TL with Streptococcus spp. and AN, CIP, EF and LS with Clostridium spp.. Most of the isolates showed the higher resistance to B, CL, L, N, NB, OX, P, PB Te and VA. The MIC range of the major isolates for AN, CIP, NOR, LS FM and GM showing the relatively high sensitivity were measured. Compared with other drugs, CIP and LS showed the lower MIC of ≤0.06∼128 ㎍/㎖ and ≤0.06∼≥512 ㎍/㎖, respectively.

일부 도시지역 노인들의 우울과 일상생활능력에 관련된 요인

박원규,이태용,정용준,오장균,이동배,조영채 충남대학교 의과대학 의학연구소 2002 충남의대잡지 Vol.29 No.1

This study was performed to provide the basic data and related factors available for the assessment of the degree of ADL and depression among older persons, manifesting their physical and psychological status. The subjects included the old people aged more than 65 years old who reside in urban areas. The interviews were delivered to 693 individuals in Taejon metropolitan city during the two-month period from June to July, 2000, and Zung Self-Rating Depression Scale and IADL developed by Older American Resources and Services of Duke medical college, were employed for the assessment of depression and IADL, respectively. The collected data were statistically processed by SPSSWIN(ver 10.0) and led to the following results. The degree of depression among 635 subjects showed that 26.6% had mild depression, 8.2% moderate and 0.6% severe while 64.6% were normal. In terms of IADL, 6.9% had impossible IADL when not aided by others, 37.0% needed some help, 56.0% possible IADL, and the higher depression scores, the significantly higher IADL scores. Based on general characteristics, depression scores were significantly higher in the female group, the older, the lower educated, the ones without cohabitants, without a spouse, without making expenses of living on their own, and IADL scores were lower, meaning the lower capability for performing daily activities, in the male group, the older, the higher educated, the group without a spouse, without making expenses of living on their own. Based on daily activities and psychological characteristics, depression scores were higher in the group who don't go out, who don't have recreational or friendly gatherings, who aren't satisfied with the lives of the past or present, who have a sense of isolation, and who don't have the will to live, and IADL scores were lower in the group who don't go out, who don't have recreational or friendly gatherings, who aren't satisfied with the lives of the past or present, who have a sense of isolation. and who don't have the will to live. Multiple regression analysis revealed that the explanatory variable having effects on depression symptoms included, with the explanatory power of 57.5%, the confidence in life, the degree of education, the subjective index of health, the sense of isolation, the exercise, the degree of IADL, the gender, the level of satisfaction with life, whether they eat breakfast or not, the concern about life, the smoking, the going out, the presence of spouse or not. The explanatory variables having effects, with the explanatory power of 28.9%, on IADL scores included the degree of depression symptoms, the age, the presence of friends or not, the frequency of outgoing, the level of education, the degree of obesity, the confidence in life, the level of satisfaction with life, the drinking or not. The study results showed that the higher depression scores had a tendency to be associated with the decreased capabilities of performing daily activities, and that the factors related to depression and/or IADL, included the confidence in life, the level of education, and the level of satisfaction with life. However, the more detailed and decisive results on the factors related to depression and IADL of the older persons are considered to require continuing efforts and comparisons among a variety of the older population.