Anti-allergic effects of a nonameric peptide isolated from the intestine gastrointestinal digests of abalone (Haliotis discus hannai) in activated HMC-1 human mast cells

KO, SEOK-CHUN,LEE, DAE-SUNG,PARK, WON SUN,YOO, JONG SU,YIM, MI-JIN,QIAN, ZHONG-JI,LEE, CHANG-MIN,OH, JUNGHWAN,JUNG, WON-KYO,CHOI, IL-WHAN Spandidos Publications 2016 INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE Vol.37 No.1

<P>The aim of the present study was to examine whether the intestine gastrointestinal (GI) digests of abalone [Halioti s discus hannai (H. discus hannai)] modulate inflammatory responses and to elucidate the mechanisms involved. The GI digests of the abalone intestines were fractionated into fractions I (>10 kDa), 11 (5-10 kDa) and III (<5 kDa). Of the abalone intestine GI digests (AIGIDs), fraction III inhibited the passive cutaneous anaphylaxis (PCA) reaction in mice. Subsequently, a bioactive peptide [abalone intestine GI digest peptide (AIGIDP)] isolated from fraction III was determined to be 1175.2 Da, and the amino acid sequence was found to be PFNQGTFAS. We noted that the purified nonameric peptide (AIGIDP) attenuated the phorbol-12-myristate 13-acetate plus calcium ionophore A23187 (PMACI)-induced histamine release and the production of pro-inflammatory cytokines, such as tumor necrosis factor-alpha (TNF-alpha), interleukin (IL)-1 beta and IL-6 in human mast cells (HMC-1 cells). In addition, we also noted that AIGIDP inhibited the PMACI-induced activation of nuclear factor-kappa B (NF-kappa B) by suppressing I kappa B alpha phosphorylation and that it suppressed the production of cytokines by decreasing the phosphorylation of JNK. The findings of our study indicate that AIGIDP exerts a modulatory, anti-allergic effect on mast cell-mediated inflammatory diseases.</P>

Pulmonary inflammation caused by silica dioxide nanoparticles in mice via TXNIP/NLRP3 signaling pathway

Je‑Oh Lim,Je‑Won Ko,Tae‑Yang Jung,Woong‑Il Kim,So‑Won Pak,In‑Sik Shin,Won‑Kee Yun,Hyoung‑Chin Kim,Jeong‑Doo Heo,Jong‑Choon Kim 대한독성 유전단백체 학회 2020 Molecular & cellular toxicology Vol.16 No.3

Background Silica dioxide nanoparticles (SiONPs) have been used for various medical applications, including therapeutics and imaging, and the use of SiONPs has increased gradually over the years. However, despite an increase in the use of SiONPs, not much is known about mechanism of action of SiONPs and their pulmonary toxicity. Objective The present study investigated the pulmonary toxicity of SiONPs and explored the underlying mechanism of action, primarily focusing on thioredoxin-interacting protein (TXNIP)/NOD-like receptor pyrin domain-containing 3 (NLRP3) in SiONPs-treated mice. We investigated the toxic effects of SiONPs in the lung of BALB/c mice administered 5, 10, and 20 mg/kg SiONPs for 3 days. Results Exposure to SiONPs markedly increased inflammatory cell counts, including those of neutrophils and macrophages, and levels of inflammatory mediators, such as interleukin (IL)-1β, IL-6, and tumor necrosis factor-α in a dose-dependent manner in the bronchoalveolar lavage fluid. Moreover, the inflammation was verified upon histopathological analysis. In addition, exposure to SiONPs increased the expression of TXNIP in a dose-dependent manner and, in turn, upregulated NLRP3 inflammasome proteins, which subsequently induced IL-1β production. Conclusion Collectively, exposure to SiONPs induced inflammation in the lungs of mice, which resulted in the activation of IL-1β production via the TXNIP-NLRP3 axis. Our results provide useful information on the pulmonary toxicity induced by SiONPs and provide insights into the underlying mechanism of action.

고려홍삼의 수지상세포 활성화 효과

김도순(Do-Soon Kim),박정은(Jueng-Eun Park),서권일(Kwon-Il Seo),고성룡(Sung-Ryong Ko),이종원(Jong-Won Lee),도재호(Jae-Ho Do),이성태(Sung-Tae Yee) 고려인삼학회 2006 Journal of Ginseng Research Vol.30 No.3

본 연구에서는 정관장 홍삼의 물(water) extract, 식용발효 주정 extract 및 홍삼 추출물로부터 분리 제조한 crude saponin을 이용하여 면역반응을 매개하는 수지상세포의 활성효과에 대하여 알아보았다. 그 결과 홍삼시료 중, crude saponin 100 ㎍/㎖을 처리하였을 때 수지상세포의 세포표면분자인 MHC class II, CD40, CD80, CD86의 발현이 증가하였으며, phagocytosis는 감소하였다. 또한 홍삼시료를 처리한 수지상세포와 allogeneic T세포를 함께 배양하였을 때, 홍삼시료의 물 extract, 식용발효주정 extract, crude saponin 모두 allogeneic T세포의 증식반응을 유도하였고, IL-2와 IFN-γ의 생산량을 증가시키는 것을 확인하였다. 또한 CD4? syngeneic T세포와 CD8? syngeneic T세포의 반응에서도 T세포의 증식반응을 높게 유도하였으며, CD4? syngeneic T세포에서 IL-2와 IFN-γ의 생산량을 증가시키고, CD8? syngeneic T세포에서는 IFN-γ 생산량을 증가시키는 것을 확인하였다. 이상의 결과로 crude saponin의 경우 수지상세포의 세포표면 공동자극분자의 발현을 유도하고 성숙을 유도함으로써 T세포의 활성을 증진시키는 것으로 생각되며, 물 extract와 식용발효주정 extract는 crude saponin과는 다른 기작으로 T세포 활성화를 유도하는 것을 알 수 있었다. 따라서 실험에 사용한 홍삼시료, 즉 물 extract, 식용발효주정 extract, crude saponin 모두 수지상세포의 활성을 유도하는 물질로써 암항원 특이적 T세포 활성화를 이용한 항암치료에 이용할 수 있는 가능성이 있다고 사료된다. Ginseng is a medicinal herb widely used in Asian countries. Dendritic cells(DCs) play a pivotal role in the initiation of T cell-mediated immune responses, making them an attractive cellular adjuvant for use in cancer vaccines. In this study, we examined the effects of Red-ginseng(water extract, edible and fermented ethyl alcohol extract, crude saponin) on the DCs phenotypic and functional maturation. Immature DCs were cultured in the presence of GM-CSF and IL-4, and the generated immature DCs were stimulated by water extract, edible and fermented ethyl alcohol extract, crude saponin and LPS, respectively, for 24hours. The expression of surface co-stimulatory molecules, including MHC(major histocompatibility complex) class II, CD40, CD80 and CD86, was increased on DCs that were stimulated with crude saponin, but antigen-uptake capacity was decreased. The antigen-presenting capacity of Red-ginseng extracts-treated DCs as analyzed by allogeneic T cells proliferation and IL-2, IFN-γ production was increased. Furthermore, CD4? and CD8? syngeneic T cell(OVA-specific) proliferation and IFN-γ production was significantly increased. However, CD4? syngeneic T cell secreted higher levels of IL-2 in responding but not CD8? syngeneic T cell. These results indicate the immunomodulatory properties of Red-ginseng extracts, which might be therapeutically useful in the control of cancers and immunodeficient diseases through the up-regulation of DCs maturation.

열환경인공기후실의 설계

고경태,정성일,황병렬,박종일,김경훈 경희대학교 자연과학종합연구원 1997 자연과학논문집 Vol.3 No.-

The purpose of this study is the design of the climate chamber. Experiments was performed in a climate chamber that was running Under Floor Air-Conditioning System (UFAC). The chamber has a diffuser in the middle of the floor, and exhausts on the ceiling, simulating underfloor air conditioning system.

Identification of AFLP Marker Linked to a SCN Resistant Gene in Soybean

Ko,Mi-Suk,Kim,Myung-Sik,Han,Soung-Jin,Chung,Jong-Il,Kang,Jin-Ho 한국자원식물학회 2002 Plant Resources Vol.5 No.3

The soybean cyst nematode (Heterodera glycines Inchinoe; SCN) is a devastating pest of soybean and is responsible for significant losses in yield. The use of resistant cultivars is the effective method to reduce or eliminate SCN damage. The objective of this research is to identify AFLP markers linked to the SCN resistant genes. Bulked genomic DNA was made from resistant and susceptible genotypes to SCN and a total of 19 primer combinations were used. About 31 fragments were detected per primer combination. The banding patterns were readily distinguished in resistant and susceptible bulked genotypes. Polymorphic fragments were detected between resistant and susceptible bulked genotypes in the primer combination of CGT/GGC, CAG/GTG and CTC/GAG. In primer combinations of CGT/GGC and CAG/GTG, bulked resistant genotype produced a polymorphic bands. However, in primer of CTC/GAG, bulked susceptible genotype produced a polymorphic fragments. Three AFLP markers identified as a polymorphic fragments between bulked genomic DNA were mapped in 85 F2 population. Among them, only two markers, CGT/GGC and CTC/GAG, was linked and was mapped. Broad application of AFLP marker would be possible for improving resistant cultivars to SCN.

평야지재배 조생종 벼의 이화학적 및 식미특성 분석

정진일,김기영,최윤희,최원영,고종철,오명규,홍하철,이승엽,이명철 한국국제농업개발학회 2004 韓國國際農業開發學會誌 Vol.16 No.4

평야지재배 적응 조생종 벼 품종의 선발 및 품종 육성의 기초 자료로 활용코자 극조생종(1품종)과 조생종(10품종) 및 준조생(3품종)등 총 14품종을 산간지(운봉)와 평야지(익산)에 지역별 표준재배법으로 공시한 결과, 1. 극조생종 및 조생종들은 감온성이 커 산간지에서 출수가 빠르나, 준조생종에 속하는 상미벼, 영덕34호, 히또메보레 등은 감광성이 감온성보다 커, 평야지재배에서 4일정도 빨리 출수하였다. 2. 수량성은 대부분의 품종들이 산간지재배에 비해 평야지 재배에서 높았고 대체로 출수가 빠른 품종일수록 지역간의 수량차가 크게 나타났으며, 출수가 다소 늦은 준조생종들은 산간지와 평야지의 재배에서 수량차가 크지 않아 산간지 뿐만 아니라 평야지에서도 적응성이 높았다. 3. 단백질함량은 숙기가 다소 늦은 품종일수록 낮은 경향을 보였고 산간지보다는 평야지에서 낮았으며, 품종간에는 오대벼, 운봉30호, 영덕34호, 신석조생, 상미벼 등이 함유량이 낮았다. 4. 식미치는 산간지재배가 평야지에 비해 보다 높게 나타났고, 출수가 늦을수록 높았으며, 지역간 차가 크게 나타난 품종은 극조생종인 키라라397이었고, 출수가 늦은 상미벼와 히또메보레는 차가 적었다. 5. 산온도와 수량과의 상관은 산간지에서만 영향이 있을 뿐 평야에서는 영향이 적고 일사량과 일조시수가 수량에 보다 상관성이 높았으며, 식미치는 적산온도와는 부의상관, 생육 일수와는 정상관을 보였다. This experiment was carried out to clarify the difference of rice yield and the quality of rice in southern plan area and in alpine area. Yield and quality of fourteen early varieties were investigated under the different transplanting dates and planting densities in southern plan area(Iksan) and in alpine area(Unbong). In the alpine area, extremely early variety and early varieties headed early compared to the plain area, but medium early varieties headed about 4 days late unlike the alpine area. The yield difference of most early varieties including Kilara 397 of extremely early flowering variety were greater in plain area than in alpine. The different of yield between plain and alpine area was less in varieties with Sinseogjosaeng, Odaebyeo, Akidagomachi, and Yeungdeuk34. The rate of protein was lower in plain area than alpine and more lately flowering than early, but palatability of milled rice was higher at the alpine area and more lately flowering varieties. Correlation coefficients between yield and meterological data during maturity, yield shower the significant correlation with integrated solar radiation and sunshine hours at cultured in southern plan area, but it showed only cumulative temperature in alpine area. Palatability of miller rice was positive correlation at growth duration but negative correlation at cumulative temperature.

유육종증 1례

전수영,정운태,황성보,류종철,최수전,고일향 인제대학교 1993 仁濟醫學 Vol.14 No.4

저자들은 폐결핵 추정하에 치료를 받았던 Sarcoidosis 환자 1례를 경험했기에 문헌적 고찰과 함께 보고하는 바이다. Sarcoidosis is a granulomatous disease of unknown etiology In which pulmonary manifestations typically predominate with protean extrapulmonary and systemic manifestations. In sarcoidosis, pulmonary symptoms include dyspnea on exertion, non-productive cough and wheezing. Typical radiologic finding is a diffuse infiltration with or without bilateral symmetric hilar lymphadenopathy. Sarcoidosis should be differentiated with pulmonary tuberculosis because of its clincal and radiological slmilarity in Korea, an endemic area of pulmonary tuberculosis. We report a case of sarcoidosis patient whose symptoms were not improved by treatment with anti-tuberculous medication, but by steroid therapy following the diagnosis of sarcoidosis made by biopsy.

A New Rice Cultivar with High Grain Quality, Adaptability to Reclaimed Saline Soil and Multi-Disease Resistance, "Cheongho"

Jong Cheol Ko,Ki Yong Ha,Ki Young Kim,Kyu Seong Lee,Mun Sik Shin,Jeong Kwon Nam,Jin Il Choung,Jae Kil Lee,Jae Kwon Ko,Bo Kyeong Kim,Weon Young Choi,Nam Hyun Back,Sang Soo Kim 한국육종학회 2006 한국육종학회지 Vol.38 No.4

Research Institute (HARI), NTCS, RDA, in 2004. This cultivar has a short grain shape and about 123 days growth duration from trans-planting to harvesting under the reclaimed saline area of the south-western and the mid-western coastal plain and Honam plai

전력소비자의 단기수요예측을 위한 전력소비패턴과 환경요인과의 관계 분석

고종민(Jong-Min Ko),송재주(Jae-Ju Song),김영일(Young-Il Kim),양일권(Il-Kwon Yang) 대한전기학회 2010 전기학회논문지 Vol.59 No.11

Studies on the development of various energy management programs and real-time bidirectional information infrastructures have been actively conducted to promote the reduction of power demands and CO2 emissions effectively. In the conventional energy management programs, the demand response program that can transition or transfer the power use spontaneously for power prices and other signals has been largely used throughout the inside and outside of the country. For measuring the effect of such demand response program, it is necessary to exactly estimate short-term loads. In this study, the power consumption patterns in both individual and group consumers were analyzed to estimate the exact short-term loads, and the relationship between the actual power consumption and seasonal factors was also analyzed.

상시수요응답(Day Ahead Dernand Response) 운영에서의 CBL 활용방안 연구

高鍾旻(Jong-Min Ko),梁日權(Il-Kwon Yang),宋在周(Jae-Ju Song),陳成日(Sung-Il Jin) 대한전기학회 2009 전기학회논문지 Vol.58 No.1

In this study firstly we survey the calculation method and the characteristics of the way of estimating CBL(Customer BaseLine Load) that is important calculation tool for DRP internationally. Also we analyze the power consumption pattern using the 15 minutes load profiles of about 120,000 customers in domestic. Based on this pattern, we provide the CBL calculation method that can be utilized in DRP to save the cost, and analyze the accuracy of the CBL calculation proposed in this paper through the simulation.