Carbon monoxide prevents TNF-α-induced eNOS downregulation by inhibiting NF-κB-responsive miR-155-5p biogenesis

Choi, Seunghwan,Kim, Joohwan,Kim, Ji-Hee,Lee, Dong-Keon,Park, Wonjin,Park, Minsik,Kim, Suji,Hwang, Jong Yun,Won, Moo-Ho,Choi, Yoon Kyung,Ryoo, Sungwoo,Ha, Kwon-Soo,Kwon, Young-Guen,Kim, Young-Myeong Nature Publishing Group 2017 Experimental and molecular medicine Vol.49 No.11

<P>Heme oxygenase-1-derived carbon monoxide prevents inflammatory vascular disorders. To date, there is no clear evidence that HO-1/CO prevents endothelial dysfunction associated with the downregulation of endothelial NO synthesis in human endothelial cells stimulated with TNF-α. Here, we found that the CO-releasing compound CORM-2 prevented TNF-α-mediated decreases in eNOS expression and NO/cGMP production, without affecting eNOS promoter activity, by maintaining the functional activity of the <I>eNOS</I> mRNA 3′-untranslated region. By contrast, CORM-2 inhibited MIR155HG expression and miR-155-5p biogenesis in TNF-α-stimulated endothelial cells, resulting in recovery of the 3′-UTR activity of <I>eNOS</I> mRNA, a target of miR-155-5p. The beneficial effect of CORM-2 was blocked by an NF-κB inhibitor, a miR-155-5p mimic, a HO-1 inhibitor and siRNA against HO-1, indicating that CO rescues TNF-α-induced eNOS downregulation through NF-κB-responsive miR-155-5p expression via HO-1 induction; similar protective effects of ectopic HO-1 expression and bilirubin were observed in endothelial cells treated with TNF-α. Moreover, heme degradation products, except iron and <I>N</I>-acetylcysteine prevented H<SUB>2</SUB>O<SUB>2</SUB>-mediated miR-155-5p biogenesis and eNOS downregulation. These data demonstrate that CO prevents TNF-α-mediated eNOS downregulation by inhibiting redox-sensitive miR-155-5p biogenesis through a positive forward circuit between CO and HO-1 induction. This circuit may play an important preventive role in inflammatory endothelial dysfunction associated with human vascular diseases.</P>

원위 대퇴골의 고립성 골연골종에서 발생한 속발성 연골 육종 1례 보고

정필현,황장수,강석,김용민,오형호,채동주,김종필 동국대학교 경주대학 1996 東國論集 Vol.15 No.-

연골육종은 비교적 서서히 자라며 늦게 전이하는 악성 연골 종양으로 알려져 있다. 골 연골종에서 발생한 속발성 연골육종은 발생빈도는 드물며 타부위로의 전이가 적고 악성도가 낮은 경우가 많아 적합한 수술적 치료로서 좋은 예후를 보이는 것으로 보고되고 있다. 본 동국대학교 정형외과학 교실에서는 우측 대퇴골 원위부 골간단부에 생긴 고립성 골 연골종에서 이차적으로 발생한 연골육종을 치험한 바 문헌고찰과 함께 보고하는 바이다. A case of Secondary Chondrosarcoma Arising from Solitary Osteochondroma of the Distal Femur Department of Orthopaedic Surgery, College of Medicine, DongGuk University Phil Hyun Chung M.D., Jung Su Hwang M.D., Suk Kang M.D., Yang Mln Kim M.D., Hyung Ho Oh M.D., Dong Ju Chae M.D., Jong Pil Kim M.D. Chondrpsarcoma is a malignant cartilage-forming tumor that grows slowly and metastasis of this tumor occurs in late stage. Secondary chondrosarcomas arising from a solitary osteocartilaginous exostosis are rare and those have a better prognosis than other chondrosarcomas, and they rarely metastasize. We present our experience with a case of secondary chondrpsarcoma arising in solitary osteochondroma of distal metaphysis of right femur with clinical details.

서울의 Penicillinase Producing Neisseria gonorrhoeae 발생빈도(1998)

김재홍,김준호,반재용,이정우,황성주,정준규,정성태,강진문,조흔정,홍창의,정혜신,이한승,김이선,이봉길,이종호,선영우,한기덕,윤성필,이성훈,안종성,박석범,문승현,조항래,김형섭,류지호,황재영,박준홍,손상욱 한양대학교 의과대학 2001 한양의대 학술지 Vol.21 No.1

In recent years, gonorrhea has been pandemic and remains one of the most common STDs in the world, especially in developing countries. For the detection of a more effective therapeutic regimen and assessing the prevalence of Penicillinase Producing Neisseria gonorrhoeae(PPNG), we have been trying to study the patients who have visited the Venereal Disease Clinic of Choong-Ku Public Health Center in Seoul since 1980 by menas of the chromogenic cephalosporin method. In 1998, 93 strians of N. genorrhoeae were isolated, among which 60(64.5%) were PPNG. The prevalence of PPNG in Seoul, which had been decreased to 39% in 1996 after a peak of 74.3% in 1993, is increased to 64.5% in 1998.

초 두랄루민(Al 2024)의 정적인 크리프 거동

황경충,윤종호 한국공작기계학회 2004 한국공작기계학회 추계학술대회논문집 Vol.2004 No.-

Super-duralumin has widely been used as the part materials of aerospace and automobile industry because it has high specific strength and also is light. But, we have little design data about the creep behaviors of the alloy. Therefore, in this study, every creep test under four constant stress conditions have been conducted for four temperature conditions. A series of creep tests had been performed to get the basic design data and life prediction of super-duralumin products and we have gotten the following results. First, the stress exponents showed the descending trend as the test temperatures increase. Secondly, the creep activation energy gradually decreased as the stresses become bigger. Thirdly, the constant of Larson-Miller parameters on this alloy was estimated about 6. And last, the fractographs at the creep rupture showed both the brittle fracture due to the transgranular rupture.

포괄수가지불제도 적용에 따른 산부인과 진료수입의 변화

황태연,유병철,정귀언,정수진,김성준,배기택,손혜숙,이종태,전진호,엄상화 인제대학교 2001 仁濟醫學 Vol.22 No.1

Objectives: Under the fee-for-service system, Korean hospitals have been suffering from the worsening profitability caused by law medical care fees. To overcome the difficulties, they have maximized the quantity of medical services and made relatively large investment in developing new medical services using high-priced medical equipment, medicines and medical supplies which were not covered by medical insurance system rather than reduced running costs. Materials and Methods: This study was made to analyze and forecast the revenue change of the department of Obstetrics and gynecology of an university hospital in Seoul, expecting the implementation of DRG system in 2000. Results: The results were as follows : 1.During the first two days of hospitalization. 41% of total medical service fee was occurred, which was thought that most of the check-ups and operations were made during this period. After two days of hospitalization, the new occurrence of medical service fee tended to reduce. 2.Out of total medical service fee, 67% of admission fee was occurred after 5 days of hospitalization. This was because medical service fees in obstetrics and gynecology department occurred during the first 5 days of hospitalization. 3.Out of fees for operation, treatment, medical supplies and blood transfusion. 71% was occurred during the first two days. 4. In case of fees for examination, 50% was occurred during the first two days. 5.A total of 53% of fees for medication and injection was occurred during the first 5 days. Conclusions: By the implementation of the DRG system, the income is forecast to increase by 800 won to 310,000 won by the disease group of obstetrics and gynecology department. To increase hospital income with the implementation of DRG system, the results of this study suggest that examinations should be done at out-patient departments before the hospitalization of patient, the discharge of patient should be noticed in advance to reduce the period of hospitalization, and admission appointment system should be implemented for the immediate operation and delivery.

사회복귀시설의 현황과 전망

황태연,이종국,고영,이주호,지성학 용인정신의학연구소 1997 용인정신의학보 Vol.4 No.1

Interleukin-2가 Mouse 폐장의 폐포간 중격의 미세구조에 미치는 영향

황세진,김종우,정호삼,이규식 한양대학교 의과대학 1990 한양의대 학술지 Vol.10 No.1

Interleukin-2, a kind of lymphokine which is produced by activated T-lymphocytes during an immune response, is known to act not only as a growth factor for many cells of immune system but stimulates differentiation of cells of immune system. Although interleukin-2 is a useful agent for cancer immunotherapy and inhibiting agent of tumor cell metastasis, administration of interleukin-2 to cancer patient may cause various side effects such as pulmonary edema due to capillary leakage. In this experiment the author pursued the effects of interleukin-2 on ultrastructure of interalveolar septum in lung of mouse. Healthy male mice weighing about 20gm were used as experimental animals and divided into control group and experimental group. During the experiment water and foods were given ad libitum. Animals of control group were injected 0.2 ml of distilled water and animals of experimental grouup were injected 2 million units per kg body weight of interleukin-2 intraperitoneally Experimental animals were sacrified at 3 hours, 6 hours, 12 hours, 24 hours, 48 hours and 72 hours after administration and lung specimens were obtained. All specimens, which were prefixed in 2% glutaradehyde-2.5% paraformaldehyde and postfixed in osmium tetraoxide were dehydrated and embedded in Epon Araldite 502. Ultrathinsection about 600-800 in thickness, and uranyl acetate and lead citrate double stain were performed and all electron microscopic specimens were observed with JEM 100cx-II electron microscope. The results obtained were as follows: 1. The type I pneumocyte of interalveolar septum of lungs in interleukin-2 treated mice were swollen, separated from basal lamina. 2. Capillary endothelial cell of interalveolar septum of lungs in interleukin-2 treated mice were swollen, and pinocytic vesicles in endothelial cell were enlarged and increased in number. 3. Lamellar bodies of type II pneumocytes of interalveolar septum of lungs in interleukin-2 treated mice were enlarged, and vacuolized. 4. Mitochondria and microvilli of type II pneumocytes of interalveolar septum of lungs in interleukin-2 treated mice were decreased in number. It is consequently suggested that interleukin-2 induces intracellular edema of both the type I pneumocyte and the endothelial cell and damages to type II pneumocyte of interalveolar septum of lung in mice but alveolar epithelial cells and endothelial cells are recover as time goes by.

신경세포 시냅스에서 Shank2, Homer 1b와 PLC-β3의 신호전달 복합체 형성

황종익,신금주,류성호,서판길 한국뇌학회 2001 한국뇌학회지 Vol.1 No.2

포스포리파아제 C-베타(phospholipase C-β: PLC-β) 동위효소는 G-proteins-coupled receptors(GPCR)와 그의 신호전달 매개체인 heterotrimeric G proteins에 의해 활성화된다. PLC-β는 효소적 활성에 필요한 영역 외에 카르복시 말단에 PSD-95/Dlg/ZO-1 (PDZ)-binding motif를 가진다. 이 motif는 PLC-β가 PDZ domain을 가진 단백질들과 결합함으로써 GPCR 매개 신호전달에서 특이적인 역할을 수행할 가능성을 제시한다. 이에 본 연구자들은 효모 two-hybrid assay를 통하여 Shank2가 PLC-β3와 결합함을 이전에 확인하였다. 본 연구에서는, 면역침전반응을 통해 두 단백질의 결합은 PLC-β3의 카르복시 말단의 PDZ-binding motif를 통하여 이루어짐을 확인하였고 신경세포의 시냅스에서 mGluR1 및 IP3 수용체와 결합하는 Homer 1b 와 Shank2가 PLC-β3와 함께 위치하는 것을 발견하였다. 이러한 결과는 전자현미경을 이용하여 Homer 1b와 Shank2가 위치하는 PSD 영역에서 PLC-β3가 존재함을 확인하고 mGluR1과 Shank2, Homer 1b, PLC-β3가 해마와 소뇌의 많은 부분에서 동시에 발현되는 것을 확인함으로써 다시 한번 입증되었다. 결과를 종합해 볼 때, 이들 세 단백질은 같은 신경세포의 시냅스에서 발현되어 복합체를 형성함으로써 mGluR1에 의해 매개되는 신호전달을 보다 효율적으로 수행할 수 있을 것으로 예상된다. Phospholipase C-β isotypes activated by G protein-coupled receptors (GPCR) and heterotrimeric G proteins carry a PSD-95/Dlg/ZO-1 (PDZ)-binding motif in their carboxyl terminus. This motif may enable PLC-β isotypes to play specific roles in GPCR signaling through interacting with PDZ-containing proteins. We identified Shank2 as a PLC-β3 binding protein, using yeast two-hybrid system in the previous study, and here we characterized the binding of PLC-β3 with Shank2. Immunoprecipitation study showed that Shank2 interacts with PDZ-binding motif of PLC-β3. Moreover, PLC-β3 was colocalized with Shank2 and Homer 1b that interacts with mGluR1 and IP3 receptor on the synapse of primary-cultured neuronal cells. Immunogold EM also showed that PLC-β3 immunoreactivity was detected at the PSD of the CA1 pyramidal neurons. In several regions of hippocampus and cerebellum, the expression pattern of Shank2 was similar to that of mGluR1, Homer 1b, and PLC-β3. These results suggest that PLC-β3, Shank2, and Homer 1b may act as active intermediates in mGluR1-mediated signal transduction by forming signaling complex in neuronal synapses.

절삭공구 팁의 TiCN코팅 특성

황경층,윤종호,최재하,김성청 충북대학교 산업과학기술연구소 2002 산업과학기술연구 논문집 Vol.16 No.2

As the machine tool industry progresses, the performance of cutting tools also needs to be of good quality and specialized. If the existing metal cutting tool tip is coated properly, its life would be longer and the machining of a difficult-to-cut material also could be possible. For the development of the high quality cutting tool, the applicabilities of TiCN coating deposited by PVD on the cutting insert were experimentally investigated. The various analyses such as SEM micrograph, XRD pattern, AFM surface morphology, and hardness were performed to cross-check the possibility and availability of TiCN coated tool in the field.

인코넬 690 합금의 크리프거동

황경충,윤종호,최재하,김성청 한국공작기계학회 2002 한국생산제조학회지 Vol.11 No.4

Inconel 690 alloy has widely been used in power plant and high temperature facilities because it has high thermal resistance and toughness. But, we have little design data about the creep behaviors of the alloy. Therefore, in this study, an apparatus has been designed and built for conduction creep tests under constant load conditions. A series of creep tests on Inconel 690 alloy have been performed to get the basic design data and life prediction of inconel products and we have gotten the following results. First, the stress exponents decrease as the test temperatures increase. Secondly, the creep activation energy gradually decreases as the stresses becomes bigger. Thirdly, the constant of Larson-Moller parameters on this alloy is estimated about 10. And last, the fractographs at the creep rupture show both the ductile and the brittle fracture according to the creep conditions.