Inhibition of SKP2 Sensitizes Bromocriptine-Induced Apoptosis in Human Prolactinoma Cells

Jinxiang Huang,Fenglin Zhang,Lei Jiang,Guohan Hu,Wei Sun,Chenran Zhang,Xuehua Ding 대한암학회 2017 Cancer Research and Treatment Vol.49 No.2

Purpose Prolactinoma (prolactin-secreting pituitary adenoma) is one of the most common estrogenrelated functional pituitary tumors. As an agonist of the dopamine D2 receptor, bromocriptine is used widely to inhibit prolactinoma progression. On the other hand, it is not always effective in clinical application. Although a dopamine D2 receptor deficiency contributes to the impaired efficiency of bromocriptine therapy to some extent, it is unknown whether there some other underlying mechanisms leading to bromocriptine resistance in prolactinoma treatment. That is the main point addressed in this project. Materials and Methods Human prolactinoma samples were used to analyze the S-phase kinase associated protein 2 (SKP2) expression level. Nutlin-3/adriamycin/cisplatin-treated GH3 and MMQ cells were used to analyze apoptosis in SKP2 overexpression or knockdown cells. SKP2 expression and the interaction partners of SKP2 were also detected after a bromocriptine treatment in 293T. Apoptosis was analyzed in C25 and bromocriptine-treated GH3 cells. Results Compared to normal pituitary samples, most prolactinoma samples exhibit higher levels of SKP2 expression, which could inhibit apoptosis in a p53-dependent manner. In addition, the bromocriptine treatment prolonged the half-life of SKP2 and resulted in SKP2 overexpression to a greater extent, which in turn compromised its pro-apoptotic effect. As a result, the bromocriptine treatment combined with C25 (a SKP2 inhibitor) led to the maximal apoptosis of human prolactinoma cells. Conclusion These findings indicated that SKP2 inhibition sensitized the prolactinoma cells to bromocriptine and helped promote apoptosis. Moreover, a combined treatment of bromocriptine and C25 may contribute to the maximal apoptosis of human prolactinoma cells.

CFD Numerical Simulation of Wind Field and Vehicle Aerodynamic Characteristics on Truss Bridge Deck under Crosswind

Mingjin Zhang,Jinxiang Zhang,Junting Long,Yongle Li,Yulin Zou,Dianguo Yin 대한토목학회 2022 KSCE Journal of Civil Engineering Vol.26 No.12

Due to the complexity and changeability of the wind field in deep-cut gorges, the vehicles on the bridge deck are easily affected by a strong crosswind. Thus, to accurately evaluate the wind field characteristics of a suspension bridge deck, the wind profiles of wind speed and angle of attack (AoA) and the vehicle aerodynamic parameters were investigated by the CFD. The results show that the shape of wind speed profile is mainly controlled by the AoA but less affected by the Reynolds number. The main girder's shielding effect can accelerate the local wind field, and the closer to the windward lane, the less the interference; thus, a suitable location of measuring points to represent the incoming flow is found. Furthermore, the equivalent wind speed based on the equivalent side force is generally larger than the value based on the rolling moment, and the responding value is greatly affected by the AoA. In addition, the vortex is a time-dependent phenomenon, the averaged flow field produced less force in the wake, but the high local wind speed variations may affect the traffic unfavorably. The results provide an essential reference significance for studying the local wind field characteristics of the bridge deck.

^(64)Zn and ^(67)Zn (n,α) Reactions in the MeV Neutron Energy Region

Guohui Zhang,Jiaguo Zhang,Hao Wu,Jiaming Liu,Jinxiang Chen,Yuri Gledenov,Milana Sedysheva,Gonchigdorj Khuukhenkhuu,Pavel Szalanski 한국물리학회 2011 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.59 No.23

Nuclear reactions of ^(64)Zn(n,α)^(61)Ni and ^(67)Zn(n,α)^(64)Ni are important because they are gas pro-duction reactions and zinc is a reactor constituent element with a signicant fraction. But the cross section data of these reactions are scanty because the residual nuclei ^(61)Ni and ^(64)Ni are stable and the commonly used activation method is not feasible. In the present work, differential cross sections and angleintegrated cross sections were measured for the ^(64)Zn(n,α)^(61)Ni reaction at neutron energies of 2.5, 4.0, 5.0, 5.5, and 6.0 MeV and cross sections of the ^(67)Zn(n,α)^(64)Ni reaction were measured at 4.0, 5.0, and 6.0 MeV. A twin gridded ionization chamber was employed as alpha particle detector. Experiments were performed at the 4.5 MV Van de Graaff accelerator of Peking University. Monoenergetic neutrons of 2.5 MeV were produced through the T(p, n)^3He reaction with a solid Ti-T target, and those of other energies were produced through the D(d, n)^3He reaction with a deuterium gas target. Absolute neutron fluxes were determined through the ^(238)U(n, f) reaction and a BF^3 long counter was used as the neutron flux monitor. Present results are compared with other measurements and evaluations.

Wind characteristics in the high-altitude difference at bridge site by wind tunnel tests

Mingjin Zhang,Jinxiang Zhang,Yong-Le Li,Jisheng Yu,Jingyu Zhang,Lianhuo Wu 한국풍공학회 2020 Wind and Structures, An International Journal (WAS Vol.30 No.6

With the development of economy and construction technology, more and more bridges are built in complex mountainous areas. Accurate assessment of wind parameters is important in bridge construction at complex terrain. In order to investigate the wind characteristics in the high-altitude difference area, a complex mountain terrain model with the scale of 1:2000 was built. By using the method of wind tunnel tests, the study of wind characteristics including mean wind characteristics and turbulence characteristics was carried out. The results show: The wind direction is affected significant by the topography, the dominant wind direction is usually parallel to the river. Due to the sheltering effect of the mountain near the bridge, the wind speed and wind attack angle along the bridge are both uneven which is different from that at flat terrain. In addition, different from flat terrain, the wind attack angle is mostly negative. The wind profiles obey exponential law and logarithmic law. And the fitting coefficient is consistent with the code which means that it is feasible to use the method of wind tunnel test to simulate complex terrain. As for turbulence characteristics, the turbulence intensity is also related to the topography. Increases sheltering effect of mountain increases the degree of breaking up the large-scale vortices, thereby increasing the turbulence intensity. Also, the value of turbulence intensity ratio is different from the recommended values in the code. The conclusions of this study can provide basis for further wind resistance design of the bridge.

EFFECT OF THIOPHENE IN BITHIAZOLE-BRIDGED SENSITIZERS ON THE PERFORMANCE OF DYE-SENSITIZED SOLAR CELLS

XIAOYU ZHANG,JIANLI HUA,JINXIANG HE,WENJUN WU,HANS AGREN,XIN LI,FULING GUO 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2014 NANO Vol.9 No.5

In this paper, we have designed and synthesized four bithiazole-bridged sensitizers (BT-T2,TBT-T2, BT-T3 and TBT-T3) with triphenylamine and indoline as the donor segment andapplied them to dye-sensitized solar cells (DSSCs). For triphenylamine-based sensitizers as BT-T2 and TBT-T2, adding one thiophene unit between triphenylamine donor and bithiazolemoiety not only led to bathochromic shift of the maximum absorption and increase of molarextinction coe±cient, but also enhanced the photovoltaic conversion e±ciency from 7.12% ofBT-T2 to 7.51% of TBT-T2. But for indoline-based sensitizers as BT-T3 and TBT-T3,adding one thiophene unit between indoline donor and bithiazole moiety resulted in hypochro-matic shift instead of bathochromic shift. We employed the density functional theory (DFT)calculations to further investigate the in°uence of the thiophene unit on their optical and elec-tronic properties and photovoltaic performance of corresponding DSSC devices. Given theresults, a reasonable explanation is the introduction of thiophene unit suppressed the intramo-lecular charge transfer and charge separation in the conjugation system of indoline-based sen-sitizer, which led to the hypochromatic shift of the maximum absorption wavelength and ¯nallythe low Jsc . Since the Jsc dropped sharply from 15.26 mAcm? 2 to 4.52 mAcm? 2 , the photovoltaicconversion e±ciency decreased dramatically from 7.86% to 1.93%.

Experimental study on solidification of uranium tailings by microbial grouting combined with electroosmosis

Deng Jinxiang,Li Mengjie,Tian Yakun,Wu Lingling,Hu Lin,Zhang Zhijun,Zheng Huaimiao 한국원자력학회 2023 Nuclear Engineering and Technology Vol.55 No.12

The present microbial reinforcement of rock and soil exhibits limitations, such as uneven reinforcement effectiveness and low calcium carbonate generation rate, resulting in limited solidification strength. This study introduces electroosmosis as a standard microbial grouting reinforcement technique and investigates its solidification effects on microbial-reinforced uranium tailings. The most effective electroosmosis effect on uranium tailings occurs under a potential gradient of 1.25 V/cm. The findings indicate that a weak electric field can effectively promote microbial growth and biological activity and accelerate bacterial metabolism. The largest calcium carbonate production occurred under the gradient of 0.5 V/cm, featuring a good crystal combination and the best cementation effect. Staged electroosmosis and electrode conversion efficiently drive the migration of anions and cations. Under electroosmosis, the cohesion of uranium tailings reinforced by microorganisms increased by 37.3% and 64.8% compared to those reinforced by common microorganisms and undisturbed uranium tailings, respectively. The internal friction angle is also improved, significantly enhancing the uniformity of reinforcement and a denser and stronger microscopic structure. This research demonstrates that MICP technology enhances the solidification effects and uniformity of uranium tailings, providing a novel approach to maintaining the safety and stability of uranium tailings dams

Response of Glucocorticoid Receptor Alpha and Histone Deacetylase 2 to Glucocorticoid Treatment Predicts the Prognosis of Sudden Sensorineural Hearing Loss

Xiuling Zhang,Jinxiang Chen,Ziwen Gao,Hui Qi,Yanhong Dai,Wandong She 대한이비인후과학회 2019 Clinical and Experimental Otorhinolaryngology Vol.12 No.4

Objectives. To investigate glucocorticoid receptor (GR) and histone deacetylase 2 (HDAC2) gene expression and protein levels in peripheral blood mononuclear cells (PBMCs) of patients with severe or profound sudden sensorineural hearing loss (SSNHL) and to explore the roles of GRs and HDAC2 in glucocorticoid (GC) insensitivity. Methods. Fifty-five severe or profound SSNHL patients were enrolled in the study. According to hearing improvement after GC treatment, patients were assigned into two groups: GC-sensitive and GC-resistant. A normal reference group included 20 healthy volunteers without hearing loss. Quantitative real-time polymerase chain reaction and Western blot analyses were used to detect the relative expression of GRα, GRβ, and HDAC2 in PBMCs at the mRNA and protein levels. Results. The protein levels of GRs and HDAC2 in PBMCs of SSNHL patients were lower than the normal reference values before GC treatment. Compared with the GC-resistant group, both the mRNA and protein levels of GRα and HDAC2 were significantly increased in the GC-sensitive group after GC treatment. Conclusion. A lack of GRα and HDAC2 induction following steroid treatment in GC-resistant SSNHL patients may play a fundamental mechanistic role in GC insensitivity. Response of GRα and HDAC2 to steroid treatment may, thus, predict the prognosis of hearing improvement in SSNHL patients.

Optimization of the Structural Parameters of the Vertical Trabeculae Beetle Elytron Plate Based on the Mechanical and Thermal Insulation Properties

Zhijie Zhang,Elbashiry Mohamed Adam Elsafi,Jinxiang Chen,Peixing Wei,Yaqin Fu 대한토목학회 2020 KSCE JOURNAL OF CIVIL ENGINEERING Vol.24 No.12

According to the excellent structure of the beetle elytron plate (BEP), a new type of bionic prefabricated wallboard with vertical trabeculae and honeycomb walls (the vertical trabeculae beetle elytron plate: VBEPsc) was proposed. The relationships between the main structural parameters, including the concrete frame thickness (T), honeycomb wall thickness (t), trabecula number (N) and trabecular radius (R), and the performance of the VBEPsc were analyzed. In addition, to improve the mechanical and insulation performance of the VBEPsc, an initial optimal design was determined. The results showed that 1) in addition to the mechanical properties of the VBEPsc being mainly affected by T, as is known, N had an apparent impact on the stiffness of the VBEPsc; the insulation performance was affected greatly by T, N, and t. 2) Under the given conditions, the mechanical properties of the optimized VBEPsc met the requirements for bearing capacities and serviceability; The insulation performance of the optimized VBEPsc met the requirements for self-insulating walls of category II buildings in areas that are cold in winter and hot in summer. In addition, a double-layer mold and complete production technology for VBEPsc were proposed. These works started a discussion for the application of such prefabricated wallboards.

A Study of Radio Voice Signal Based on the Time Delay Estimation

Xinrui Liu,Jinxiang Chen,Xianbo He,Wei Li,Gangyuan Zhang 보안공학연구지원센터 2016 International Journal of Hybrid Information Techno Vol.9 No.9

The wave of voice signal is narrow and unstable. Besides, it has a non-continuity in time domain and an inconsistent decline of the radio channel, especially the short wave channel, which makes estimating the time delay of the radio’s voice signal very difficult. To solve such problems, the authors creatively combine the voice activity detection with the short-segment processing technology. Meanwhile, by using Weighted Least Squares Phase Fitting method to work out the time delay, we conduct a research in the time delay estimation of radio’s voice signal. The laboratory finding finally shows that this method is far better than the typical Generalized Correlation Method for estimation of time delay.

Facile One-Pot Synthesis and Optical Properties of Quinary Wurtzite Cu3ZnInSnS6 Nanocrystals

Dehui Li,Chen Zhang,Yajie Zhang,Weichen Qi,Jinxiang Dong,Jing Yang,Zhidong Qiu 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2016 NANO Vol.11 No.11

Nearly monodisperse bullet-like Cu3ZnInSnS6 (CZITS) nanocrystals with wurtzite structure were successfully synthesized through optimized noninjection method. The structure, composition, morphology and optical properties of CZITS nanocrystals were characterized by X-ray diffraction, energy dispersive spectrometry, transmission electron microscopy and UV-Vis–NIR absorption. Their suitable bandgap and photoresponsive behavior indicate a high potential application in the field of solar cells. The growth mechanism of the as-synthesized CZITS nanocrystals was preliminarily discussed. It was found that the formation of CZITS could be separated into two steps: nucleation of Cu7S4 and growth of the CZITS main body.