Parallel Compression and Decompression of DNA Sequence Reads in FASTQ Format

Jingjing Zheng,Ting Wang 보안공학연구지원센터 2014 International Journal of Hybrid Information Techno Vol.7 No.4

Inactivation of Stat3 and crosstalk of miRNA155-5p and FOXO3a contribute to the induction of IGFBP1 expression by beta-elemene in human lung cancer

Fang Zheng,Qing Tang,Xiao-hua Zheng,JingJing Wu,HaiDing Huang,Haibo Zhang,Swei Sunny Hann 생화학분자생물학회 2018 Experimental and molecular medicine Vol.50 No.-

β-Elemene, an active component of natural plants, has been shown to exhibit anticancer properties. However, the detailed mechanism underlying these effects has yet to be determined. In this study, we show that β-elemene inhibits the growth of lung cancer cells. Mechanistically, we found that β-elemene decreased the phosphorylation of signal transducer and activator of transcription 3 (Stat3) and miRNA155-5p mRNA but induced the protein expression of human forkhead box class O (FOXO)3a; the latter two were abrogated in cells with overexpressed Stat3. Notably, miRNA155-5p mimics reduced FOXO3a luciferase reporter activity in the 3-UTR region and protein expression, whereas overexpressed FOXO3a countered the reduction of the miRNA155-5p levels by β-elemene. Moreover, β-elemene increased the mRNA and protein expression levels as well as promoter activity of insulin-like growth factor-binding protein 1 (IGFBP1); this finding was not observed in cells with a silenced FOXO3a gene and miRNA155-5p mimics. Finally, silencing of IGFBP1 blocked β-elemene-inhibited cell growth. Similar findings were observed in vivo. In summary, our results indicate that β-elemene increases IGFBP1 gene expression via inactivation of Stat3 followed by a reciprocal interaction between miRNA155-5p and FOXO3a. This effect leads to inhibition of human lung cancer cell growth. These findings reveal a novel molecular mechanism underlying the inhibitory effects of β-elemene on lung cancer cells.

High-fat-diet impaired mitochondrial function of cumulus cells but improved the efficiency of parthenogenetic embryonic quality in mice

Jingjing Li,Shuang Wang,Bo Wang,Hao Wei,Xin Liu,Jun Hao,Yanping Duan,Jinlian Hua,Xiaomin Zheng,Xiuliang Feng,Xingrong Yan 한국통합생물학회 2018 Animal cells and systems Vol.22 No.4

Global human health has been compromised by high-fat diets. This study aimed to investigate the relationship between a high-fat diet and parthenogenetic embryo quality. Mice fed a high-fat or a normal diet was used as treated or control groups, respectively. Estradiol (E2), total cholesterol (TC) and total triglyceride (TG) were detected by Enzyme-Linked ImmunoSorbent Assay (ELISA). Cumulus-oocyte complexes (COCs) were collected from the mice in the treated and control groups. The ultrastructure of COCs, the expression level of genes involved in mitochondrial and nuclear functions in cumulus cells and oocytes quality were evaluated with transmission electron microscopy, real-time quantitative polymerase chain reaction (RT-PCR) and artificial parthenogenesis, respectively. The results showed that the efficiency of parthenogenetic embryonic development in vitro was significantly higher in the treated group than in the control group (p < .05). The expression level of genes involved in mitochondrial function was lower in cumulus cells from the treated group than that from the control group (p < .05). The estradiol and cholesterol level in the serum and the expression level of P450 arom were higher in the treated group than the control group (p < .05). The reactive oxygen species (ROS) level was higher in culumus cells from the treated group than the control group, while the mitochondrial membrane potential was lower in cumulus cells from the treated group (p < .05). Accumulation of lipid droplets was only in cumulus but in oocyte, the results demonstrated that mitochondrial functions were impaired by a high-fat diet, but parthenogenetic embryonic development in vitro was improved, in controllable range of damage for the body.

Thermal Conductivity of Loess: Experimental Studies and Empirical Model

Jingjing Wang,Juntao Deng,Jianguo Zheng,Tiehang Wang,Yongtang Yu 대한토목학회 2024 KSCE Journal of Civil Engineering Vol.28 No.2

Soil thermal conductivity is an important indicator for developing and utilizing geothermal resources. In this study, the impact of underground environmental factors on the thermal conductivity of loess was explored by studying the thermal conductivity of saturated and unsaturated loess under varying water content, dry density, and temperature using the Hot Disk thermal constant analyzer. The results show that the thermal conductivity of unsaturated undisturbed and remolded loess presents different growth trends as the water content increases. When the water content is less than 9%, the thermal conductivity of undisturbed soil increases slowly. The thermal conductivity of unsaturated loess gradually increases with temperature. When the temperature rises above 30oC, the latent heat transfer of steam gradually strengthens, accelerating the increase in thermal conductivity, which is most noticeable at intermediate saturation. However, the thermal conductivity of saturated loess rises slowly as the temperature rises. A weighted geometric average model is proposed in this study to predict the thermal conductivity of loess under temperature conditions, considering the effects of soil water content, dry density, and mineral content. The model accuracy was corroborated by the measured soil thermal conductivity and the data collected from six regions.

Protective role of endothelial calpain knockout in lipopolysaccharide-induced acute kidney injury via attenuation of the p38-iNOS pathway and NO/ROS production

Zhifeng Liu,Jingjing Ji,Dong Zheng,Lei Su,Tianqing Peng,Jing Tang 생화학분자생물학회 2020 Experimental and molecular medicine Vol.52 No.-

To explore the role of calpain and its signaling pathway in lipopolysaccharide (LPS)-induced acute kidney injury (AKI), animal models of endotoxemia were established by administration of LPS to mice with endothelial-specific Capn4 knockout (TEK/Capn4−/−), mice with calpastatin (an endogenous calpain inhibitor) overexpression (TgCAST) and mice with myeloid-specific Capn4 knockout (LYZ/Capn4−/−). Mouse pulmonary microvascular endothelial cells (PMECs) were used as a model of the microvascular endothelium and were stimulated with LPS. Renal function, renal inducible nitric oxide synthase (iNOS) and endothelial NOS (eNOS) expression, cellular apoptosis, plasma and renal levels of NO and reactive oxygen species (ROS), and phosphorylation of mitogenactivated protein kinase (MAPK) family members (p38, ERK1/2, and JNK1/2) were examined. Moreover, a calpain inhibitor, calpastatin overexpression adenoviruses and MAPK inhibitors were used. Significant renal dysfunction was induced by LPS stimulation, and recovery was observed in TEK/Capn4−/− and Tg-CAST mice but not in LYZ/ Capn4−/− mice. Endothelial Capn4 knockout also abrogated the LPS-induced increases in renal iNOS expression, caspase-3 activity and apoptosis and plasma and renal NO and ROS levels but did not obviously affect renal eNOS expression. Moreover, LPS increased both calpain and caspase-3 activity, and only the expression of iNOS in PMECs was accompanied by increased phosphorylation of p38 and JNK. Inhibiting calpain activity or p38 phosphorylation alleviated the increased iNOS expression, NO/ROS production, and cellular apoptosis induced by LPS. These results suggest that endothelial calpain plays a protective role in LPS-induced AKI by inhibiting p38 phosphorylation, thus attenuating iNOS expression and further decreasing NO and ROS overproduction-induced endothelial apoptosis.

Preparation, Curing, and Properties of Boron-Containing Bisphenol-S Formaldehyde Resin/o-Cresol Formaldehyde Epoxy Resin/Nano-SiO2 Composites

Weihong Wu,Jingjing Leng,Zheng Wang,Hongqiang Qu,Jungang Gao 한국고분자학회 2016 Macromolecular Research Vol.24 No.3

Boron-containing bisphenol-S formaldehyde resin (BBPSFR) with different amounts of nano-SiO2 by in situ formation was used to cure o-cresol formaldehyde epoxy resin (o-CFER). The curing kinetics, dynamic mechanical properties, and thermal stability of BBPSFR/o-CFER/nano-SiO2 composites (BCS) were investigated by differential scanning calorimetry (DSC), dynamic mechanical analysis (DMA), thermogravimetry (TG), and thermogravimetrymass spectrometry (TGMS). Morphology of nano-SiO2-containing BBPSFR and glass fiber laminates of the BCS were characterized by transmission electron microscopy (TEM) and scanning electron microscopy (SEM). The mechanical properties and electrical properties were also determined. The results showed that nano-SiO2 accelerated the curing process and decreased the curing temperature; the non-isothermal curing kinetics of the BCS can be described by the two-parameter (m, n) Šesták-Berggren kinetic model, and the average value of m was 0.32 and n was 1.00. The thermal stability was enhanced by the addition of nano-SiO2, especially at higher temperatures, and the residual weight increased with increasing nano-SiO2 content. Incorporation of 6 wt% of nano-SiO2 increased the impact strength from 105 to 149 kJ/m2 and storage modulus at ambient from 6.85 to 12.7 GPa, and the TEM photograph of which showed that nano-SiO2 particles (about 50 nm) were dispersed in the matrix more uniformly. The volume resistance, Rv, and dielectric constant, ε, slightly increased when the nano-SiO2 content was 3 wt%.

In-process Measurement and Geometric Error Fusion Control of Discontinuous Surface Based on Bayesian Theory

Manyi Cao,Peng Zheng,Dongliang Liu,Jingjing Chang,Linna Zhang 한국정밀공학회 2021 International Journal of Precision Engineering and Vol.22 No.4

For workpieces with discontinuous surface characteristics such as shafts with keyways, obtaining continuous and valid measurement data is essential for assessing machining quality. In this paper, a Bayesian approach based on prior knowledge is proposed to evaluate the geometric characteristics of the discontinuous surface. Effective measurement data for discontinues surface is supplemented by accurate Bayesian parameter estimation. By using the supplemented data, the dimensional error and roundness error can be calculated. Using the in-process measurement method, the process progress can be adjusted in time according to the error value to realize the fusion control of dimension and roundness. Experimental investigations for machining multi-keyway shaft are carried out based on the proposed method. The results show that grinding workpieces with discontinuous surface by using the in-process measurement method based on Bayesian theory can significantly improve product quality and efficiency.

Internal Mammary Sentinel Lymph Node Biopsy after Neoadjuvant Chemotherapy in Breast Cancer

Zhao Bi,Peng Chen,Jingjing Liu,Yanbing Liu,Pengfei Qiu,Qifeng Yang,Weizhen Zheng,Yongsheng Wang 한국유방암학회 2018 Journal of breast cancer Vol.21 No.4

Purpose: The definition of nodal pathologic complete response (pCR) after a neoadjuvant chemotherapy (NAC) just included the evaluation of axillary lymph node (ALN) without internal mammary lymph node. This study aimed to evaluate the feasibility of internal mammary-sentinel lymph node biopsy (IM-SLNB) in patients with breast cancer who underwent NAC. Methods: From November 2011 to 2017, 179 patients with primary breast cancer who underwent operation after NAC were included in this study. All patients received radiotracer injection with modified injection technology. IM-SLNB would be performed on patients with internal mammary sentinel lymph node (IMSLN) visualization. Results: Among the 158 patients with cN+ disease, the rate of nodal pCR was 36.1% (57/158). Among the 179 patients, the visualization rate of IMSLN was 31.8% (57/179) and was 12.3% (7/57) and 87.7% (50/57) among those with cN0 and cN+ disease, respectively. Furthermore, the detection rate of IMSLN was 31.3% (56/179). The success rate of IM-SLNB was 98.2% (56/57). The IMSLN metastasis rate was 7.1% (4/56), and all of them were accompanied by ALN metastasis. The number of positive ALNs in patients with IMSLN metastasis was 3, 6, 8, and 9. The pathology nodal stage had been changed from pN1/ pN2 to pN3b. The pathology stage had been changed from IIA/ IIIA to IIIC. Conclusion: Patients with visualization of IMSLN should perform IM-SLNB after NAC, especially for patients with cN+ disease, in order to complete lymph nodal staging. IM-SLNB could further improve the definition of nodal pCR and guide the internal mammary node irradiation.

Refolding Behavior of Urea-Induced Denaturation Collagen

Xu Wei,Yanqiu Zhao,Jingjing Zheng,Qin Cao,Sheng Li,Lang He,Benmei Wei,Juntao Zhang,Chengzhi Xu,Haibo Wang 한국고분자학회 2021 Macromolecular Research Vol.29 No.6

Exploration of the denaturation and refolding of natural collagen is important for the application of collagen and its denatured products. In this study, using urea as a denaturant, we prepared a denatured natural collagen product and analyzed its structural changes. The denaturation treatment severely destroyed the triple helix conformation of collagen, but had no significant effect on the primary structure of its α chains or the covalent cross-linking between α chains. Next, we observed the refolding behavior of the denatured collagen by removing urea through dialysis. We found that the denatured collagen products from different sources (grass carp skin, bovine tendon) all showed a reconstruction of the triple helix conformation up to 60-75% of the value of natural collagen during the refolding process. The telopeptide did not significantly promote triple helix reconstruction. In conclusion, the reconstruction of the α chains did not perfectly occur in a “head-to-head, tail-to-tail” manner in refolded collagen, as each α chain was participating in the reconstruction of multiple triple helix domains. The refolded collagen still had weak self-assembly ability and formed a unique network-like structure containing small interlaced and closely combined fibers, which shows favorable cell compatibility and potential applications.

Characteristics of long-range transported PM2.5 at a coastal city using the single particle aerosol mass spectrometry

Qiuliang Cai,Lei Tong,Jingjing Zhang,Jie Zheng,Mengmeng He,Jiamei Lin,Xiaoqiu Chen,Hang Xiao 대한환경공학회 2019 Environmental Engineering Research Vol.24 No.4

Air pollution has attracted ever-increasing attention because of its substantial influence on air quality and human health. To better understand the characteristics of long-range transported pollution, the single particle chemical composition and size were investigated by the single particle aerosol mass spectrometry in Fuzhou, China from 17th to 22nd January, 2016. The results showed that the haze was mainly caused by the transport of cold air mass under higher wind speed (10 m·s<SUP>-1</SUP>) from the Yangtze River Delta region to Fuzhou. The number concentration elevated from 1,000 to 4,500 #·h<SUP>-1</SUP>, and the composition of mobile source and secondary aerosol increased from 24.3% to 30.9% and from 16.0% to 22.5%, respectively. Then, the haze was eliminated by the clean air mass from the sea as indicated by a sharp decrease of particle number concentration from 4,500 to 1,000 #·h<SUP>-1</SUP>. The composition of secondary aerosol and mobile sources decreased from 29.3% to 23.5% and from 30.9% to 23.1%, respectively. The particles with the size ranging from 0.5 to 1.5 ㎛ were mainly in the accumulation mode. The stationary source, mobile source, and secondary aerosol contributed to over 70% of the potential sources. These results will help to understand the physical and chemical characteristics of long- range transported pollutants.