Draft Genome Assembly and Annotation for Cutaneotrichosporon dermatis NICC30027, an Oleaginous Yeast Capable of Simultaneous Glucose and Xylose Assimilation

Wang Laiyou,Guo Shuxian,Zeng Bo,Wang Shanshan,Chen Yan,Cheng Shuang,Liu Bingbing,Wang Chunyan,Wang Yu,Meng Qingshan 한국균학회 2022 Mycobiology Vol.50 No.1

The identification of oleaginous yeast species capable of simultaneously utilizing xylose and glucose as substrates to generate value-added biological products is an area of key eco- nomic interest. We have previously demonstrated that the Cutaneotrichosporon dermatis NICC30027 yeast strain is capable of simultaneously assimilating both xylose and glucose, resulting in considerable lipid accumulation. However, as no high-quality genome sequenc- ing data or associated annotations for this strain are available at present, it remains chal- lenging to study the metabolic mechanisms underlying this phenotype. Herein, we report a 39,305,439 bp draft genome assembly for C. dermatis NICC30027 comprised of 37 scaffolds, with 60.15% GC content. Within this genome, we identified 524 tRNAs, 142 sRNAs, 53 miRNAs, 28 snRNAs, and eight rRNA clusters. Moreover, repeat sequences totaling 1,032,129 bp in length were identified (2.63% of the genome), as were 14,238 unigenes that were 1,789.35 bp in length on average (64.82% of the genome). The NCBI non-redundant protein sequences (NR) database was employed to successfully annotate 11,795 of these unigenes, while 3,621 and 11,902 were annotated with the Swiss-Prot and TrEMBL databases, respectively. Unigenes were additionally subjected to pathway enrichment analyses using the Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG), Cluster of Orthologous Groups of proteins (COG), Clusters of orthologous groups for eukaryotic com- plete genomes (KOG), and Non-supervised Orthologous Groups (eggNOG) databases. Together, these results provide a foundation for future studies aimed at clarifying the mech- anistic basis for the ability of C. dermatis NICC30027 to simultaneously utilize glucose and xylose to synthesize lipids.

Association between a p73 Gene Polymorphism and Genetic Susceptibility to Non-small Cell Lung Cancer in the South of China

Wang, Shuang-Shuang,Guo, Hai-Yan,Dong, Lin-Li,Zhu, Xiang-Qian,Ma, Liang,Li, Wen,Tang, Jian-Xin Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.23

Background: This study aimed to identify any association between the p73 gene G4C14-to-A4T14 polymorphism and risk of non-small cell lung cancer (NSCLC) in the south of China. Materials and Methods: We genotyped the p73 gene polymorphism of peripheral blood DNA from 168 patients with NSCLC and 195 normal controls using HRM (high resolution melting) and PCR-CTPP (polymerase chain reaction with confronting two-pair primers). Results: The results of genotyping by HRM and PCR-CTPP were consistent with direct sequencing, the p73 genotype distribution in 168 lung cancer patients being as follows: GC/GC 101 cases (60.1%), GC/AT 59 cases (35.1%), AT/AT 8 cases (4.8%). The carriers of AT/AT genotype had a significantly reduced risk of NSCLC (OR=0.370; 95%CI: 0.170-0.806; p=0.010) as compared with non-carriers. However, we found no relations between p73 genotypes and histological type (p=0.798, $x^2=0.452$), tumor stage (p=0.806, $x^2=0.806$), or lymph node metastasis (p=0.578, $x^2=1.098$). Conclusions: Our findings suggest that the p73 G4C14-to-A4T14 polymorphism may be a modifier of NSCLC susceptibility in the Chinese population.

Right-to-Left Displacement of an Airgun Lead Bullet after Transorbital Entry into the Skull Complicated by Posttraumatic Epilepsy : A Case Report

Chao-bin Wang,Hui Wang,Jun-shuang Zhao,Ze-jun Wu,Hao-dong Liu,Chao-jia Wang,An-rong Li,Dawei Wang,Juntao Hu 대한신경외과학회 2023 Journal of Korean neurosurgical society Vol.66 No.5

Penetrating head injury is a serious open cranial injury. In civilians, it is often caused by non-missile, low velocity flying objects that penetrate the skull through a weak cranial structure, forming intracranial foreign bodies. The intracranial foreign body can be displaced due to its special quality, shape, and location. In this paper, we report a rare case of right-to-left displacement of an airgun lead bullet after transorbital entry into the skull complicated by posttraumatic epilepsy, as a reminder to colleagues that intracranial metal foreign bodies maybe displaced intraoperatively. In addition, we have found that the presence of intracranial metallic foreign bodies may be a factor for the posttraumatic epilepsy, and their timely removal appears to be beneficial for epilepsy control.

ATM Signaling Pathway Is Implicated in the SMYD3-mediated Proliferation and Migration of Gastric Cancer Cells

Wang, Lei,Wang, Qiu-Tong,Liu, Yu-Peng,Dong, Qing-Qing,Hu, Hai-Jie,Miao, Zhi,Li, Shuang,Liu, Yong,Zhou, Hao,Zhang, Tong-Cun,Ma, Wen-Jian,Luo, Xue-Gang The Korean Gastric Cancer Association 2017 Journal of gastric cancer Vol.17 No.4

Purpose: We previously found that the histone methyltransferase suppressor of variegation, enhancer of zeste, trithorax and myeloid-nervy-deformed epidermal autoregulatory factor-1 domain-containing protein 3 (SMYD3) is a potential independent predictive factor or prognostic factor for overall survival in gastric cancer patients, but its roles seem to differ from those in other cancers. Therefore, in this study, the detailed functions of SMYD3 in cell proliferation and migration in gastric cancer were examined. Materials and Methods: SMYD3 was overexpressed or suppressed by transfection with an expression plasmid or siRNA, and a wound healing migration assay and Transwell assay were performed to detect the migration and invasion ability of gastric cancer cells. Additionally, an MTT assay and clonogenic assay were performed to evaluate cell proliferation, and a cell cycle analysis was performed by propidium iodide staining. Furthermore, the expression of genes implicated in the ataxia telangiectasia mutated (ATM) pathway and proteins involved in cell cycle regulation were detected by polymerase chain reaction and western blot analyses. Results: Compared with control cells, gastric cancer cells transfected with si-SMYD3 showed lower migration and invasion abilities (P<0.05), and the absence of SMYD3 halted cells in G2/M phase and activated the ATM pathway. Furthermore, the opposite patterns were observed when SMYD3 was elevated in normal gastric cells. Conclusions: To the best of our knowledge, this study provides the first evidence that the absence of SMYD3 could inhibit the migration, invasion, and proliferation of gastric cancer cells and halt cells in G2/M phase via the ATM-CHK2/p53-Cdc25C pathway. These findings indicated that SMYD3 plays crucial roles in the proliferation, migration, and invasion of gastric cancer cells and may be a useful therapeutic target in human gastric carcinomas.

Indoor CO₂ Adsorption Using KOH-Modified Activated Carbon in a Tubular Apparatus: The Effect of Water Content within Adsorbent

Shuang Wang,Yu-Ri Lee,Yooseob Won,Se-Eun Jeong,Jae-Yong Kim,Dong-Ho Lee,Young Cheol Park,Sung-Ho Jo,Hyungseok Nam 한국신재생에너지학회 2022 AFORE Vol.2022 No.9

Laboratory Investigation of Stress State and Grain Composition Affecting Internal Erosion in Soils Containing a Suspended Cut-off Wall

Shuang Wang,Jiansheng Chen,Jinchang Sheng,Yulong Luo 대한토목학회 2016 KSCE JOURNAL OF CIVIL ENGINEERING Vol.20 No.4

Cut-off walls have been widely used to control seepage in dams and their foundations for over 100 years. The soil at the bottom of a cut-off wall is usually under complex stress and composed of various particles with different contents. In this study, the effects of the stress state and grain composition on the evolution of internal erosion were investigated in sandy gravel foundations containing a suspended cut-off wall. The following observations were made based on the experimental results: (1) The fine-particle content of the soil sample considerably influences the evolution of internal erosion. For high fine-particle content, particles are likely to clog under the downward flow; however, for low fine-particle content, the downward flow assists the migration of fine particles. (2) Deviatoric stress considerably influences the soil structure. The soil sample is compressed under low deviatoric stress; however, it undergoes dilatancy under high deviatoric stress. (3) Dilatant behavior is also controlled by confining stress. The higher the confining stress, the higher is the deviatoric stress applied on the soil sample, which produces dilatancy, and the smaller is the volumetric expansion. The obtained results will enhance the understanding of the progression mechanism of internal erosion around suspended cut-off walls.

An Improved Predictive Functional Control with Minimum-Order Observer for Speed Control of Permanent Magnet Synchronous Motor

Wang, Shuang,Fu, Junyong,Yang, Ying,Shi, Jian The Korean Institute of Electrical Engineers 2017 Journal of Electrical Engineering & Technology Vol.12 No.1

In this paper, an improved predictive functional control (PFC) scheme for permanent magnet synchronous motor (PMSM) control system is proposed, on account of the standard PFC method cannot provides a satisfying disturbance rejection performance in the case of strong disturbances. The PFC-based method is first introduced in the control design of speed loop, since the good tracking and robustness properties of the PFC heavily depend on the accuracy of the internal model of the plant. However, in orthodox design of prediction model based control method, disturbances are not considered in the prediction model as well as the control design. A minimum-order observer (MOO) is introduced to estimate the disturbances, which structure is simple and can be realized at a low computational load. This paper adopted the MOO to observe the load torque, and the observations are then fed back into PFC model to rebuild it when considering the influence of perturbation. Therefore, an improved PFC strategy with torque compensation, called the PFC+MOO method, is presented. The validity of the proposed method was tested via simulation and experiments. Excellent results were obtained with respect to the speed trajectory tracking, stability, and disturbance rejection.

Amino acid, fatty acid, and carbohydrate metabolomic profi les with ginsenoside-induced insecticidal effi cacy against Ostrinia furnacalis (Guenee)

Shuangli Liu,Xiaohui Wang,Rui Zhang,Mingjie Song,Nanqi Zhang,Wanying Li,Yingping Wang,Yonghua Xu,Lianxue Zhang 고려인삼학회 2020 Journal of Ginseng Research Vol.44 No.4

Background: Previous studies have shown the insecticidal efficacy of ginsenosides. In the present study,we aimed to investigate the metabolic mechanism related to the inhibitory effect of panaxadiol saponins(PDSs) against the Asian corn borer Ostrinia furnacalis (Guenee). Methods: Third instar larvae of O. furnacalis were fed normal diets with different concentrations of PDSsfor 4 days. The consumption index, relative growth rate, approximate digestibility, and conversion ofingested and digested food were recorded. A targeted gas chromatographyemass spectrometry assaywas performed to detect the profiles of amino acids, fatty acids, and carbohydrates in larvae ofO. furnacalis. In addition, the activity of detoxification-related enzymes was determined. Results and Conclusions: PDSs decreased the consumption index, relative growth rate, approximate digestibility,and conversion of ingested and digested food in the 3rd instar larvae of O. furnacalis in a dosedependentmanner. PDSs decreased 15 free amino acids, 16 free fatty acids, and 5 carbohydrates andincreased the levels of palmitoleic acid, palmitic acid, and 9-octadecenoic acid in the 3rd instar larvae. The activity of detoxification-related enzymes, such as acetylcholinesterase, glutathione S-transferase,cytochrome P450, carboxylesterase, trehalase, acid phosphatase, and alkaline phosphatase, was reducedin a dose-dependent manner in the 3rd instar larvae exposed to PDSs. These data confirmed theinhibitory effect of PDSs against growth, food utilization, and detoxification in the 3rd instar larvae ofO. furnacalis and the potential for using PDSs as an efficient tool for insect pest management forO. furnacalis larvae.

Effect of packing structure on anisotropic effective thermal conductivity of thin ceramic pebble bed

Wang, Shuang,Wang, Shuai,Wu, Bowen,Lu, Yuelin,Zhang, Kefan,Chen, Hongli Korean Nuclear Society 2021 Nuclear Engineering and Technology Vol.53 No.7

Helium cooled solid breeder blanket as an important blanket candidate of the Tokamak fusion reactor uses ceramic pebble bed for tritium breeding. Considering the poor effective thermal conductivity of the ceramic breeder pebble bed, thin structure of tritium breeder pebble bed is usually adopted in the blanket design. The container wall has a great influence on the thin pebble bed packing structure, especially for the assembly of mono-sized particles, and thin pebble bed will appear anisotropic effective thermal conductivity phenomenon. In this paper, thin ceramic pebble beds composed of 1 mm diameter Li₄SiO₄ particles are generated by the EDEM 2.7. The effective thermal conductivity of different thickness pebble beds in the three-dimensional directions are analyzed by three-dimensional thermal network method. It is observed that thin Li₄SiO₄ pebble bed showing anisotropic effective thermal conductivity under the practical design size. Normally, the effective thermal conductivity along the bed vertical direction is higher than the horizontal direction due to the gravity effect. As the thickness increases from 10 mm to 40 mm, the effective thermal conductivity of the pebble bed gradually increases.

Expression and Significance of Twist and E-cadherin in Ovarian Cancer Tissues

Wang, Wen-Shuang,Yu, Shou-Li,Yang, Xing-Sheng,Chang, Shu-De,Hou, Jian-Qing Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.2

Objective: To investigate the expression of Twist and E-cadherin in ovarian cancer tissues as well as the role of epithelial-mesenchymal transformation (EMT) in ovarian cancer metastasis. Method: The expressions of Twist and E-cadherin in 54 cases of ovarian cancer and paracancerous tissues were detected by Western blottin g and reverse transcriptase polymerase chain reaction. We used RNA interference to silence Twist expression in human ovarian cancer cell line, and detected E-cadherin expression using Western blotting. Results: There was an increase in the relative abundance of Twist proteins and a decrease in E-cadherin in ovarian cancer compared with normal ovary tissues (P < 0.05). The expression levels of Twist and E-cadherin mRNA were $1.49{\pm}0.53$ and $0.82{\pm}0.24$ in ovarian cancer, and $1.14{\pm}0.38$ and $1.08{\pm}0.19$ in paracancerous tissues, respectively. The difference between the indicators in ovarian cancer and in paracancerous tissues was statistically significant (P < 0.05). When the Twist expression was silenced in an ovarian cancer cell line, the expression of the E-cadherin protein increased (P<0.05). Conclusion: The expression of Twist is upregulated, whereas that of E-cadherin is downregulated in ovarian cancer. EMT, mediated by Twist, may be correlated with ovarian cancer metastasis.