Sanguinarine impairs lysosomal function and induces ROS-dependent mitophagy and apoptosis in human hepatocellular carcinoma cells

Jingjing Wang,Qi Su,Qing Wu,Kun Chen,Asmat Ullah,Mohsin Ahmad Ghauri,Yanmin Zhang 대한약학회 2021 Archives of Pharmacal Research Vol.44 No.11

Hepatocellular carcinoma (HCC) is one of themost common tumor types globally. Despite the progressmade in surgical procedures and therapeutic options, HCCremains a considerable cause of cancer-related mortality. In this study, we investigated the antitumor eff ects of sanguinarine(Sang) on HCC and its potential mechanisms. Our fi ndings showed that Sang impairs the acidic environmentof lysosomes by inhibiting cathepsin D maturation. Inaddition, Sang inhibited the formation of autolysosomes inRFP-GFP-LC3 transfected cells, subsequently suppressinglate mitophagy. Sang also induced reactive oxygen species(ROS)-dependent autophagy and apoptosis in HCC cells,which was signifi cantly attenuated following treatment witha ROS scavenger. Further investigation using autophagyinhibitors revealed that sanguinarine-induced mitochondrialdysfunction and mitophagy led to mitochondrial apoptosisin HCC cells. Immunohistochemical staining of sanguinarine-treated xenograft samples revealed that it initiated andblocked autophagy. In summary, our fi ndings suggest thatin HCC cells, Sang impairs lysosomal function and inducesROS-dependent mitophagy and apoptosis.

Phosphorylation of Silk Fibroin via Maillard Reaction and Its Behavior of Biomimetic Mineralization

Jingjing Yuan,Qian Zhou,Ping Wang,Chao Deng,Jiugang Yuan,Qiang Wang 한국섬유공학회 2019 Fibers and polymers Vol.20 No.8

Silk fibroin (SF) has the characteristics of non-toxicity, good biocompatibility and low immunogenicity, andexhibits many potential applications in biomaterial fields. In the present work, chemical phosphorylation of SF was carriedout via the Maillard reaction using D-glucose-6-phosphate (GP), aiming at increasing the number of binding-sites forcalcium ion and promoting the biomimetic mineralization of SF films. Changes in the molecular weight of SF before andafter GP grafting were analyzed by SDS-PAGE and SEC chromatogram. Structures, morphologies, and elementalcompositions for the fibroin-based films before and after mineralization were evaluated by the means of EDS, SEM, FTIR,and XRD. The results indicated that GP was successfully grafted onto SF surfaces, companying with a slight decrease in themolecular weight. Biomimetic mineralization led to a noticeable deposition of hydroxyapatite (HAp) on the film of SF-g-GP, and higher contents of calcium and phosphorous were detected on the mineralized material, revealing thatphosphorylation promoted the mineralization processing. Meanwhile, biocompatibility of the obtained SF-g-GP/HAp wasabove the acceptable level according to ISO 10993-5-2009. The present work provides an alternative to prepare the fibroinbasedbone repair materials.

Exploration on the Development and Characteristics of Composites Painting in the Contemporary Art Ecology

Wang Jingjing 국제문화기술진흥원 2022 International Journal of Advanced Culture Technolo Vol.10 No.4

The ecological expression of art in modern society takes the harmonious developmental relationship between man and nature as an element and combines it with the various manifestations of painting as an expression of ecological artistic development. The necessary relationship for the harmonious development of nature is accurately articulated, and the ecological changes in people are expressed in integrated materials that inspire human reflection. In the pursuit of material pleasures, mankind is neglectful and indifferent to the environment. The development of composite painting in art and ecology is a process that more reveres the harmony between man and nature as well as satisfying the creative value of the work. After systematic evaluation and research, people have engaged in various structural forms of composite art painting development in the long history of art development, focusing on the integration of environmental and ecological culture.In the process of nature education going through development, the comprehensive practical development of nature education is enhanced and efforts are made to feel new ecological art ideas and new ways of valuing environmental protection. In this paper, an observational study of eco-art will be carried out, starting from the theory of hierarchical division and analyzing the contradictory relationship between man and nature. Recognize nature, understand it and feel it through eco-art painting. The analysis of the contradictory relationship between man and nature is combined with the identification of various types of information that give value to the environmental protection public, and the combination of ecological painting and nature art to achieve an upgrade of the environmental protection idea of the value of painting.

In situ synthesis of MgWO4–GO nanocomposites and their catalytic effect on the thermal decomposition of HMX, RDX and AP

Wang Jingjing,Wang Weimin,Wang Jinghua,Xu Kangzhen 한국탄소학회 2020 Carbon Letters Vol.30 No.4

For solving phase separation of nanoparticles and graphene oxide (GO) in the application process, MgWO4–GO nanocom�posites were successfully synthesized using three diferent dispersants via a facile solvothermal-assisted in situ synthesis method. The structure and morphology of the prepared samples were characterized by X-ray difraction, Scanning electron microscopy, Transmission electron microscopy, Fourier transform infrared and Raman techniques. The experimental results show that MgWO4 nanoparticles are tightly anchored on the surfaces of GO sheets and the agglomeration of MgWO4 nano�particles is signifcantly weakened. Additionally, MgWO4–GO nanocomposites are more stable than self-assembly MgWO4/ GO, which there is no separation of MgWO4 nanoparticles and GO sheets by ultrasound after 10 min. The catalytic results show that, compared with bare MgWO4, MgWO4–GO nanocomposites present better catalytic activities on the thermal decomposition of cyclotetramethylenete tranitramine (HMX), cyclotrimethylene trinitramine (RDX) and ammonium per�chlorate (AP). The enhanced catalytic activity is mainly attributed to the synergistic efect of MgWO4 nanoparticles and GO. MgWO4–GO prepared using urea as the dispersant has the smallest diameter and possesses the best catalytic action among the three MgWO4–GO nanocomposites, which make the decomposition temperature of HMX, RDX and AP reduce by 10.71, 11.09 and 66.6 °C, respectively, and the apparent activation energy of RDX decrease by 68.6 kJ mol−1

A Novel Fingerprint Localization Algorithm Based on Modifed Channel State Information Using Kalman Filter

Jingjing Wang,박준구 대한전기학회 2020 Journal of Electrical Engineering & Technology Vol.15 No.4

With the increasing demand for location-based services, indoor fngerprinting localization based on received signal strength indicator or channel state information (CSI) has become an increasingly important technique due to its low hardware requirement and high accuracy. Due to robustness against the multipath efect, frequency domain CSI of orthogonal frequency division multiplexing systems is supposed to provide an excellent positioning measurement for indoor localization. In this paper, we propose a novel fngerprint localization method based on modifed CSI using the Kalman Filter. For the ofine stage, we use modifed CSI to build a fngerprint database. In the online stage, we employ the K-nearest neighbor method for location estimation. The proposed indoor fngerprint localization scheme is implemented and validated with experiments in a representative indoor environment with commercial IEEE 802.11 NICs. Compared with existing methods, the experimental results demonstrate that the proposed method can efectively reduce positioning error.

Thermal Conductivity of Loess: Experimental Studies and Empirical Model

Jingjing Wang,Juntao Deng,Jianguo Zheng,Tiehang Wang,Yongtang Yu 대한토목학회 2024 KSCE Journal of Civil Engineering Vol.28 No.2

Soil thermal conductivity is an important indicator for developing and utilizing geothermal resources. In this study, the impact of underground environmental factors on the thermal conductivity of loess was explored by studying the thermal conductivity of saturated and unsaturated loess under varying water content, dry density, and temperature using the Hot Disk thermal constant analyzer. The results show that the thermal conductivity of unsaturated undisturbed and remolded loess presents different growth trends as the water content increases. When the water content is less than 9%, the thermal conductivity of undisturbed soil increases slowly. The thermal conductivity of unsaturated loess gradually increases with temperature. When the temperature rises above 30oC, the latent heat transfer of steam gradually strengthens, accelerating the increase in thermal conductivity, which is most noticeable at intermediate saturation. However, the thermal conductivity of saturated loess rises slowly as the temperature rises. A weighted geometric average model is proposed in this study to predict the thermal conductivity of loess under temperature conditions, considering the effects of soil water content, dry density, and mineral content. The model accuracy was corroborated by the measured soil thermal conductivity and the data collected from six regions.

High-fat-diet impaired mitochondrial function of cumulus cells but improved the efficiency of parthenogenetic embryonic quality in mice

Jingjing Li,Shuang Wang,Bo Wang,Hao Wei,Xin Liu,Jun Hao,Yanping Duan,Jinlian Hua,Xiaomin Zheng,Xiuliang Feng,Xingrong Yan 한국통합생물학회 2018 Animal cells and systems Vol.22 No.4

Global human health has been compromised by high-fat diets. This study aimed to investigate the relationship between a high-fat diet and parthenogenetic embryo quality. Mice fed a high-fat or a normal diet was used as treated or control groups, respectively. Estradiol (E2), total cholesterol (TC) and total triglyceride (TG) were detected by Enzyme-Linked ImmunoSorbent Assay (ELISA). Cumulus-oocyte complexes (COCs) were collected from the mice in the treated and control groups. The ultrastructure of COCs, the expression level of genes involved in mitochondrial and nuclear functions in cumulus cells and oocytes quality were evaluated with transmission electron microscopy, real-time quantitative polymerase chain reaction (RT-PCR) and artificial parthenogenesis, respectively. The results showed that the efficiency of parthenogenetic embryonic development in vitro was significantly higher in the treated group than in the control group (p < .05). The expression level of genes involved in mitochondrial function was lower in cumulus cells from the treated group than that from the control group (p < .05). The estradiol and cholesterol level in the serum and the expression level of P450 arom were higher in the treated group than the control group (p < .05). The reactive oxygen species (ROS) level was higher in culumus cells from the treated group than the control group, while the mitochondrial membrane potential was lower in cumulus cells from the treated group (p < .05). Accumulation of lipid droplets was only in cumulus but in oocyte, the results demonstrated that mitochondrial functions were impaired by a high-fat diet, but parthenogenetic embryonic development in vitro was improved, in controllable range of damage for the body.

Multi-objective Stochastic Time-cost-quality Optimization for Construction Projects Based on the Reliability Theory

Jingjing Wang,Zongxi Wang,Huimin Liu 대한토목학회 2023 KSCE Journal of Civil Engineering Vol.27 No.11

The management problem of projects has received more attention in recent years due to the trend towards increasing complexity in the construction industry. Time, cost and quality are three important aspects of project management. Thus, achieving a balance among the minimum project time, minimum project cost and maximum project quality can determine the success of a construction project. Moreover, the time of a project not only affects the cost but also the quality of the project. However, most research usually ignores the influence of the stochastic environments and considers the time of the project as a constant. Thus, this paper explores a new method to measure the stochastic time based on system reliability theory. Two stochastic time-cost-quality tradeoff optimization models are formulated for two construction projects with considering crossover operations or not, respectively. The optimization of the time reliability-cost-quality tradeoff for the project is achieved by using the particle swarm optimization algorithm. Finally, two illustrative examples are used to demonstrate the effectiveness of the two proposed models for a construction project with four activities.

Comparative transcriptome analysis of three chrysanthemums provides insights into flavonoid and terpenoid biosynthesis

Wang Xu,Zhang Jingjing,Liu Zhige,Wang Shuaibin,Huang Bisheng,Hu Zhigang,Liu Yifei 한국식물학회 2021 Journal of Plant Biology Vol.64 No.5

Chrysanthemum indicum var. aromaticum is an important resource plant for breeding excellent aromatic varieties of ornamental and medicinal chrysanthemums. However, the biosynthesis and regulation mechanisms of typically aromatic ingredients in C. indicum var. aromaticum are poorly understood, which restricted the utilization of this plant. Here, the transcriptome of C. indicum var. aromaticum was investigated and comparative analysis was conducted with the other two closely related chrysanthemum taxa C. indicum and C. nankingense. The global transcriptome profiles showed clear difference of gene expression patterns between the three chrysanthemums, of which 52 flavonoid biosynthesis-associated genes, 157 transcription factors, and 24 flavonoid Uridine diphosphate (UDP) -glucuronosyl and -glucosyltransferase genes were significantly differentially expressed. Furthermore, 22 differential expression genes (DEGs) in relation to the terpenoid backbone biosynthesis and 183 DEGs related to terpenoid diversification were identified. Of which, 14 genes involved into TS/CYP gene cluster showed higher expression in CIA than those in CI or CN. These results increased our understanding of flavonoid diversification and aromatic ingredients accumulation in plants, and will also help improve resource conservation and sustainable utilization of the important resource plant C. indicum var. aromaticum.

Stochastic Project Scheduling Optimization for Multi-stage Prefabricated Building Construction with Reliability Application

Jingjing Wang,Huimin Liu,Zongxi Wang 대한토목학회 2023 KSCE Journal of Civil Engineering Vol.27 No.6

This study investigates a project scheduling problem of prefabricated building (PB) construction in an uncertain environment. Different from the traditional scheduling models in PB construction, we consider a complex multi-stage cooperation system including the production, transportation and assembly (PTA) phases. In this system, both activity durations and resource amounts are stochastic variables. By applying the reliability theory to the stochastic scheduling model innovatively, we formulate a duration reliability model to maximize the probability of non-delayed project completion, within the resource constraints. As the proposed model is a non-deterministic polynomial hard (NP-hard) problem, a hybrid meta-heuristic differential evolution particle swarm optimization (DEPSO) algorithm is developed, which is utilized the mutation factor of the differential evolution (DE) algorithm in the framework of the particle swarm optimization (PSO). Finally, a real-life example of a PB construction project is used to explore the performance of the proposed DEPSO algorithm. The result shows that the hybrid algorithm DEPSO can better find the global optimal solution in the multi-dimensional optimization problem.