Optimization of Pasteurization Process of Beer for Avoiding Off-Flavor

Ji Hye Kim,Jung Hwa Yang,Do Yeob Kim,Dong youl Kim,Sang Bong Lee,Meng jingjing,Seung Won Jung,Seung Ju Lee 한국산업식품공학회 2016 학술대회 및 심포지엄 Vol.2016 No.10

The pasteurization is employed for extending shelf-life and keeping the quality of products constant. However the pasteurization undesirably accelerates the oxidation of beer which renders volatile compounds concerning off-flavor. The pasteurization conditions was optimized to avoid off-flavor of beer during pasteurization. Under the isothermal condition, thermal destruction kinetics such as D and Z value of Lactobacillus brevis which is reported the most common beer spoilage, was determined. The binomial data (detected or non-detected of off-flavor) was treated with logistic regression to estimate off-flavor development (OFD) times. The temperature dependence of OFD times was established in terms of Arrhenius relationships. Optimized pasteurization temperature and time were found at which OFD times was not detected. The constraint for optimization was that the pasteurization degree should be larger than 5 decimal reduction time. The optimization was conducted through mathematical simulation using kinetics and temperature-dependent models for microbial death and OFD times. The optimized results were validated by the corresponding experimentations, which met the requirements that the concentration of Lactobacillus brevis was 5-Log reduction and the OFD times not detected.

LincR-PPP2R5C Promotes Th2 Cell Differentiation Through PPP2R5C/PP2A by Forming an RNA–DNA Triplex in Allergic Asthma

Ji Ningfei,Chen Zhongqi,Wang Zhengxia,Sun Wei,Yuan Qi,Zhang Xijie,Jia Xinyu,Wu Jingjing,Jiang Jingxian,Song Meijuan,Xu Tingting,Liu Yanan,Ma Qiyun,Sun Zhixiao,Bao Yanmin,Zhang Mingshun,Huang Mao 대한천식알레르기학회 2024 Allergy, Asthma & Immunology Research Vol.16 No.1

Purpose: The roles and mechanisms of long noncoding RNAs (lncRNAs) in T helper 2 (Th2) differentiation from allergic asthma are poorly understood. We aimed to explore a novel lncRNA, LincR-protein phosphatase 2 regulatory subunit B' gamma (PPP2R5C), in Th2 differentiation in a mouse model of asthma. Methods: LincR-PPP2R5C from RNA-seq data of CD4+ T cells of asthma-like mice were validated and confirmed by quantitative reverse transcription polymerase chain reaction, northern blotting, nuclear and cytoplasmic separation, and fluorescence in situ hybridization (FISH). Lentiviruses encoding LincR-PPP2R5C or shRNA were used to overexpress or silence LincR-PPP2R5C in CD4+ T cells. The interactions between LincR-PPP2R5C and PPP2R5C were explored with western blotting, chromatin isolation by RNA purification assay, and fluorescence resonance energy transfer. An ovalbumin-induced acute asthma model in knockout (KO) mice (LincR-PPP2R5C KO, CD4 conditional LincR-PPP2R5C KO) was established to explore the roles of LincR-PPP2R5C in Th2 differentiation. Results: LncR-PPP2R5C was significantly higher in CD4+ T cells from asthmatic mice ex vivo and Th2 cells in vitro. The lentivirus encoding LincR-PPP2R5C suppressed Th1 differentiation; in contrast, the short hairpin RNA (shRNA) lentivirus decreased LincR-PPP2R5C and Th2 differentiation. Mechanistically, LincR-PPP2R5C deficiency suppressed the phosphatase activity of the protein phosphatase 2A (PP2A) holocomplex, resulting in a decline in Th2 differentiation. The formation of an RNA-DNA triplex between LincR-PPP2R5C and the PPP2R5C promoter enhanced PPP2R5C expression and activated PP2A. LincR-PPP2R5C KO and CD4 conditional KO decreased Th2 differentiation, airway hyperresponsiveness and inflammatory responses. Conclusions: LincR-PPP2R5C regulated PPP2R5C expression and PP2A activity by forming an RNA-DNA triplex with the PPP2R5C promoter, leading to Th2 polarization in a mouse model of acute asthma. Our data presented the first definitive evidence of lncRNAs in the regulation of Th2 cells in asthma.

Intracerebral Hemorrhage Progression Score: A Novel Risk Score to Predict Neurological Deterioration after Intracerebral Hemorrhage

Ruijun Ji,Linlin Wang,Feifei Ma,Wenjuan Wang,Yanfang Liu,Runhua Zhang,Dandan Wang,Jiaokun Jia,Hao Feng,Gaifen Liu,Yi Ju,Jingjing Lu,Xingquan Zhao 대한뇌졸중학회 2022 Journal of stroke Vol.24 No.2

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Influence of temperature, time and air flow for radical scavenging activity and total polyphenol content in coffee roasting

Seokgeun Ji,Han Sub Kwak,Younggi Choi,Jingjing Ti,Yougnseung Lee,Geun Pyo Choi,Ok Hyun Yoon,Yoonhwa Jeong 한국식품영양과학회 2014 한국식품영양과학회 학술대회발표집 Vol.2014 No.10

Impact of Various Charge States of Hydrogen on Passivation of Dislocation in Silicon

Lihui Song,Jingjing Lou,Jiayi Fu,Zhenguo Ji 대한금속·재료학회 2018 ELECTRONIC MATERIALS LETTERS Vol.14 No.5

Dislocation, one of typical crystallographic defects in silicon, is detrimental to the minority carrier lifetime of silicon wafer. Hydrogen passivation is able to reduce the recombination activity of dislocation, however, the passivation efficacy is stronglydependent on the experimental conditions. In this paper, a model based on the theory of hydrogen charge state control isproposed to explain the passivation efficacy of dislocation correlated to the peak temperature of thermal annealing and illuminationintensity. Experimental results support the prediction of the model that a mix of positively charged hydrogen andnegatively charged hydrogen at certain ratio can maximise the passivation efficacy of dislocation, leading to a better powerconversion efficiency of silicon solar cell with dislocation in it.

Mechanical Property and Microstructure of Fly Ash-Based Geopolymer Activated by Sodium Silicate

Qingfeng Lv,Jingjing Yu,Fengling Ji,Liuyang Gu,Yi Chen,Xiaokang Shan 대한토목학회 2021 KSCE JOURNAL OF CIVIL ENGINEERING Vol.25 No.5

Sodium silicate is one of the common alkali activators of geopolymers. The modulus, concentration and dosage of sodium silicate have significant effects on the activation of fly ash, the strength and microstructure of geopolymer. In this paper, unconfined compressive strength test, X-ray diffraction analysis (XRD), Fourier transform infrared light (FTIR), 29Si nuclear magnetic resonance (29Si NMR), scanning electron microscope and energy disperse spectroscopy (SEM-EDS), and physisorption experiment (BET) were carried out to study the effects of the sodium silicate modulus and dosage on the mechanical property and microstructure of fly ash-based geopolymer. The results indicated that the main product of the geopolymer activated by sodium silicate was hydrated sodium aluminosilicate (N-A-S-H). When modulus value decreased and meanwhile dosage of sodium silicate increased, the reorganization and polymerization of gel products were accelerated so that the integrity and continuity of the microstructure of geopolymer were improved, and then the strength increased. When the modulus of sodium silicate was 3.28, the maximum value of the strength was at the dosage of 10%. According to this study, it was investigated that modulus value and dosage of sodium silicate had obvious influence on the alkali- activated reaction of fly ash, which can provide an engineering reference for the special soil solidification with geopolymers.

Protective role of endothelial calpain knockout in lipopolysaccharide-induced acute kidney injury via attenuation of the p38-iNOS pathway and NO/ROS production

Zhifeng Liu,Jingjing Ji,Dong Zheng,Lei Su,Tianqing Peng,Jing Tang 생화학분자생물학회 2020 Experimental and molecular medicine Vol.52 No.-

To explore the role of calpain and its signaling pathway in lipopolysaccharide (LPS)-induced acute kidney injury (AKI), animal models of endotoxemia were established by administration of LPS to mice with endothelial-specific Capn4 knockout (TEK/Capn4−/−), mice with calpastatin (an endogenous calpain inhibitor) overexpression (TgCAST) and mice with myeloid-specific Capn4 knockout (LYZ/Capn4−/−). Mouse pulmonary microvascular endothelial cells (PMECs) were used as a model of the microvascular endothelium and were stimulated with LPS. Renal function, renal inducible nitric oxide synthase (iNOS) and endothelial NOS (eNOS) expression, cellular apoptosis, plasma and renal levels of NO and reactive oxygen species (ROS), and phosphorylation of mitogenactivated protein kinase (MAPK) family members (p38, ERK1/2, and JNK1/2) were examined. Moreover, a calpain inhibitor, calpastatin overexpression adenoviruses and MAPK inhibitors were used. Significant renal dysfunction was induced by LPS stimulation, and recovery was observed in TEK/Capn4−/− and Tg-CAST mice but not in LYZ/ Capn4−/− mice. Endothelial Capn4 knockout also abrogated the LPS-induced increases in renal iNOS expression, caspase-3 activity and apoptosis and plasma and renal NO and ROS levels but did not obviously affect renal eNOS expression. Moreover, LPS increased both calpain and caspase-3 activity, and only the expression of iNOS in PMECs was accompanied by increased phosphorylation of p38 and JNK. Inhibiting calpain activity or p38 phosphorylation alleviated the increased iNOS expression, NO/ROS production, and cellular apoptosis induced by LPS. These results suggest that endothelial calpain plays a protective role in LPS-induced AKI by inhibiting p38 phosphorylation, thus attenuating iNOS expression and further decreasing NO and ROS overproduction-induced endothelial apoptosis.

RACK1 interaction with c-Src is essential for osteoclast function

박진희,Eutteum Jeong,Jingjing Lin,고려진,Ji Hee Kim,Sol Yi,Youngjin Choi,In-Cheol Kang,Daekee Lee,SooYoungLee 생화학분자생물학회 2019 Experimental and molecular medicine Vol.51 No.-

The scaffolding protein receptor for activated C-kinase 1 (RACK1) mediates receptor activator of nuclear factor κΒ ligand (RANKL)-dependent activation of p38 MAPK in osteoclast precursors; however, the role of RACK1 in mature osteoclasts is unclear. The aim of our study was to identify the interaction between RACK1 and c-Src that is critical for osteoclast function. A RACK1 mutant protein (mutations of tyrosine 228 and 246 residues to phenylalanine; RACK1 Y228F/Y246F) did not interact with c-Src. The mutant retained its ability to differentiate into osteoclasts; however, the integrity of the RANKL-mediated cytoskeleton, bone resorption activity, and phosphorylation of c-Src was significantly decreased. Importantly, lysine 152 (K152) within the Src homology 2 (SH2) domain of c-Src is involved in RACK1 binding. The c-Src K152R mutant (mutation of lysine 152 into arginine) impaired the resorption of bone by osteoclasts. These findings not only clarify the role of the RACK1-c-Src axis as a key regulator of osteoclast function but will also help to develop new antiresorption therapies to prevent bone loss-related diseases.

Analysis of Emergency Demand Response Levels of Central Air-Conditioning

Xianjun Qi,Zongshuo Ji,Hongbin Wu,Jingjing Zhang,Hejun Yang 대한전기학회 2020 Journal of Electrical Engineering & Technology Vol.15 No.6

Central air-conditioning (CAC) is a fl exible load which can be dispatched during an emergency demand response (EDR) program. However, consumers’ trade-off between thermal comfort levels (TCLs) and profi ts will aff ect the potential of CAC. Therefore, this paper proposes a method to evaluate CAC’S response levels. An EDR optimization model simulating consumers’ trade-off between TCLs and profi ts is established and it is solved by a particle swarm optimization (PSO) algorithm. Then the defi nition of EDR levels is presented to quantitatively analyze the capability of CAC providing the EDR. Furthermore, uncertainty of EDR levels caused by stochastic initial indoor temperature is analyzed through a Monte Carlo (MC) simulation. A case study shows the rationality of the presented method, and the eff ects of weight coeffi cients, compensation prices and penalty prices on EDR levels are also analyzed.

Optimization of Self-Powered Time Temperature Indicator for the Operation and Performance

Do Yeob Kim,Dong Youl Kim,Ji Hye Kim,Meng Jingjing,Sang Bong Lee,Jung Hwa Yang,Seung Won Jung,Seung Ju Lee 한국산업식품공학회 2016 학술대회 및 심포지엄 Vol.2016 No.10

A self-powered time-temperature indicator (TTI) was optimized to enhance the performance of the TTI by modifying a biofuel cell using different immobilization, redox mediators, and status of electrode. The performance of the TTI was measured by output voltage of the TTI. The enzymes and combinations of lacasse mediators (HBT (1-hydroxybenzotriazole), Rupy (Bis-(bipyridine)-(5-aminophenanthroline) ruthenium bis (hexafluorophosphate)), MB (Methylene blue), SDP (4,4- sulfonyldiphenol)) and glucose oxidase mediators (FA (Ferroceneboxaldehyde), DBQ(2,5-dihydroxybenzoquinone), HQS (8-hydroxyquinoline-5sulfonic acid hydrate), FHFP (Ferrocenium hexafluorophosphate)) were immobilized with stabilizers (pyrrole) on a glassy carbon electrode by electrodeposition by applying a square wave, cross-linking and physical immobilization. MWCNTs were used to modify glassy carbon electrodes. MWCNTs coated electrodes produced higher output voltage than uncoated electrodes. The optimum and stable performance of the self-powered TTI was that the output voltage of 64 mV and duration time was 3hr at 25°C, when the combination of Rupy, MB for laccase mediators and HQS, FHFP for glucose oxidase mediators were immobilized on MWCNTs coated electrodes by applying a square wave method. In the application, the concentrations of enzyme and glucose were adjusted to prolong the shelf-life of TTI at much lower output voltage.