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Yoonhwa Jeong,Eul-Sang Kim,Mi-Hyoun Park,Sung-Joo Hwang,김미숙 한국식품영양과학회 2004 Journal of medicinal food Vol.7 No.2
Saengshik is a Korean “non-cooked food” that is commercially produced and marketed. Ingredients in com- mercial Saengshik include grains, vegetables, fruits, mushrooms, sea plants, and various functional botanicals. This study in- vestigated the effects of Saengshik on the survival rate of streptozotocin (STZ)-induced diabetic rats. Male Sprague-Dawley rats weighing around 190 g were randomly assigned to one of the three experimental groups: a healthy normal group (NC) and two groups with STZ-induced diabetes and fed either control diet (DC) or Saengshik diet (DS). Rats in all groups were supplied with a diet of equal energy. The animals were maintained on an experimental diet for 168 days in experiment I and for 42 days in experiment II. The body weight in the DS rats decreased less than in the DC rats in both experiments I and II. There was a trend for blood glucose level in the DS group to decrease during the experimental period in both experiments I and II. A survival rate of 50% was reached on day 49 in the DC group and on day 118 in the DS group. All rats in the DC group died by day 140, while 50% of the rats in the DS group were still alive on day 168, when experiment I was terminated. In experiment II, 50% of the DC group and 90% of the DS group survived at day 42. Saengshik did not have any influence on cholesterol levels, thiobarbituric acid-reactive substances, and activities of superoxide dismutase and glutathione peroxi- dase. These results suggest that blood glucose concentrations and the survival rate are positively affected by Saengshik feed- ing in diabetic rats.
Yoonhwa Jeong 한국식품영양과학회 2011 Preventive Nutrition and Food Science Vol.16 No.2
The purpose of this study was to elucidate the physicochemical and microbiological properties of Korean traditional rice wine, Makgeolli, supplemented with black garlic extract during fermentation. Black garlic extract was diluted with distilled water to produce 0.5% and 1.0% black garlic solutions. Those solutions were then used to make rice solutions which included 2 ㎏ rice, 40 g Nuruk (a fermentation starter), and 14 g yeast. After being mixed, the rice solutions were fermented for 7 days in a water bath at 28℃. The alcohol contents of the control, 0.5% and 1.0% black garlic Makgeolli were 16.9, 16.0, and 16.2%, respectively. Total acidity, total soluble solids, and color increased throughout the fermentation process. There was an increase of microorganisms throughout the fermentation period in all the samples. Glucose was the highest free sugar, and succinic acid was the highest organic acid detected in all the samples. Thirty nine volatile compounds were detected in black garlic Makgeolli.
Jeong Wonyoung,Kim Chulmin,Kim An-Na,Choi Younggi,Kim Jong Rae,Jeong Myeongsoo,Jeong Yoonhwa,Kim Joong-Hark 건강기능식품미래포럼 2021 건강기능식품미래포럼 학술지 Vol.1 No.4
Rapid increase of human population together with environmental changes to reduce crop yields are threatening human survival since these problems are expected to cause food shortage soon. Edible insects are getting attention as a new food resource that can solve forthcoming food shortages since they are particularly rich in protein and unsaturated fatty acids. Thus, when they are used as food, it is important to prepare the extract formulation with high yield of proteins and other nutritional components with minimal damage to them. In this study, 5 extraction methods were used, which were hot water (HW), autoclave (AT), high pressure treatment for 5 minutes (HP5), high pressure treatment for 10 minutes (HP10) and enzymatic hydrolysis (ENZ), and ten extracts were prepared by using one of these methods or combinations thereof. The extracts prepared were HW, AT, AT/ENZ, AT/ENZ/HW HP5/HW, HP5/ENZ, HP5/ENZ/HW, HP10/HW, HP10/ENZ, HP10/ENZ/HW, which were investigated for yields, molecular sizes of the components, amino acid (AA) composition and antioxidant activities. The extracts prepared using ENZ method were observed to contain high contents of total AA, high ratio of essential/non-essential AA and high antioxidant activity. Thus, ENZ method is recommended to prepare the high quality-extract of this insect.
화분에서의 조단백질 및 환원당 추출시 단백질 분해효소가 미치는 영향
최수정(Su-Jeong Choi),정윤화(Yoonhwa Jeong) 한국식품영양과학회 2004 한국식품영양과학회지 Vol.33 No.8
국내산 충매 화분인 도토리 화분과 다래 화분에 단백질 분해효소를 이용하여 화분 추출물을 제조하고, 추출물의 조단백질 함량과 환원당 함량의 변화를 알아보았다. 도토리 화분의 일반성분 분석결과 수분 5.2%, 회분 2.7%, 조지방 6.2%, 조단백질 함량은 22.3%였고, 다래화분은 수분 5.4%, 회분 2.8%, 조지방 1.8%, 조단백질 함량은 27.8%였다. Casein을 기질로 사용하여 측정한 효소의 비활성(specific activity U/mg)은 Protease S>Flavozyme>Alcalase 2.4L>Protamex> Protease P>Protease A의 순이었다. 단백질 분해효소 처리에 의한 화분 추출물의 조단백질 함량은 효소 첨가군이 대조군보다 증가했고, Alcalase 2.4L을 0.2 U, 0.5 U 첨가시에 도토리 화분 추출물은 대조군보다 각각 35.8%, 45.8%, 다래 화분 추출물은 각각 68.8%, 101.5% 증가하였다. 단백질 분해효소 처리에 의한 화분 추출물의 환원당 함량은 효소 첨가군이 대조군보다 증가했고, Protease A를 0.2 U, 0.5 U 첨가 시 도토리 화분 추출물은 대조군보다 각각 11.4%, 18.4%, 다래 화분 추출물은 대조군보다 각각 12.2%, 14.2% 증가하였다. 단백질 분해 효소 처리에 의해 화분 추출물의 조단백질과 환원당의 함량이 증가되었고, 단백질 분해 효소는 화분 추출물의 조단백질 및 환원당 함량을 높이는 방법 중의 하나로 이용될 수 있다고 사료된다. This study was conducted to increase crude protein and reducing sugar contents in pollen extracts by proteases. Four commercial neutral proteases (Alcalase 2.4L, Protamex, Flavozyme and Protease A) and two alkaline proteases (Protease S and Protease P) were used to prepare acorn and Darae pollen extracts. Contents of moisture, ash, crude protein and crude fat of acorn pollen were 5.2%, 2.7%, 6.2% and 22.3%, respectively, while those of Darae pollen were 5.4%, 2.8%, 1.8% and 27.8%, respectively. Contents of crude protein and reducing sugar in pollen extracts were increased by proteases. Alcalase 2.4L was the most effective in increasing protein contents while Protease A in increasing reducing sugar contents. It is suggested the use of proteases is one of the potential methods for increasing the contents of crude protein and reducing sugar in preparation of pollen extracts.
Involvement of PI3K and MMP1 in PDGF-induced Migration of Human Adipose-derived Stem Cells
Yoonhwa Lim,Minji Lee,Hyeju Jeong,Haekwon Kim 한국발생생물학회 2017 발생과 생식 Vol.21 No.2
Human adult stem cells have widely been examined for their clinical application including their wound healing effect in vivo. To function as therapeutic cells, however, cells must represent the ability of directed migration in response to signals. This study aimed to investigate the mechanism of platelet-derived growth factor (PDGF)-induced migration of the human abdominal adipose-derived stem cells (hADSCs) in vitro. A general matrix metalloproteinase (MMP) inhibitor or a MMP2 inhibitor significantly inhibited the PDGF-induced migration. PDGF treatment exhibited greater mRNA level and denser protein level of MMP1. The conditioned medium of PDGF-treated cells showed a caseinolytic activity of MMP1. Transfection of cells with siRNA against MMP1 significantly inhibited MMP1 expression, its caseinolytic activity, and cell migration following PDGF treatment. Phosphatidylinositol 3-kinase (PI3K) inhibitor reduced the migration by about 50% without affecting ERK and MLC proteins. Rho-associated protein kinase inhibitor mostly abolished the migration and MLC proteins. The results suggest that PDGF might signal hADSCs through PI3K, and MMP1 activity could play an important role in this PDGF-induced migration in vitro.
Involvement of PI3K and MMP1 in PDGF-induced Migration of Human Adipose-derived Stem Cells
Lim, Yoonhwa,Lee, Minji,Jeong, Hyeju,Kim, Haekwon The Korean Society of Developmental Biology 2017 발생과 생식 Vol.21 No.2
Human adult stem cells have widely been examined for their clinical application including their wound healing effect in vivo. To function as therapeutic cells, however, cells must represent the ability of directed migration in response to signals. This study aimed to investigate the mechanism of platelet-derived growth factor (PDGF)-induced migration of the human abdominal adipose-derived stem cells (hADSCs) in vitro. A general matrix metalloproteinase (MMP) inhibitor or a MMP2 inhibitor significantly inhibited the PDGF-induced migration. PDGF treatment exhibited greater mRNA level and denser protein level of MMP1. The conditioned medium of PDGF-treated cells showed a caseinolytic activity of MMP1. Transfection of cells with siRNA against MMP1 significantly inhibited MMP1 expression, its caseinolytic activity, and cell migration following PDGF treatment. Phosphatidylinositol 3-kinase (PI3K) inhibitor reduced the migration by about 50% without affecting ERK and MLC proteins. Rho-associated protein kinase inhibitor mostly abolished the migration and MLC proteins. The results suggest that PDGF might signal hADSCs through PI3K, and MMP1 activity could play an important role in this PDGF-induced migration in vitro.