Overexpression of Chalcone Isomerase (CHI) Increases Resistance Against Phytophthora sojae in Soybean

Jin-ming Zhao;Han Xing,Yang Zhou; Jian-li Huang; Xiao-li Zhang; Long-ming Zhu; Xin-fang Wang 한국식물학회 2018 Journal of Plant Biology Vol.61 No.5

Chalcone isomerase (CHI) is a key enzyme ofthe flavonoid pathway that plays crucial roles in plant responsesto various pathogens. Our previous study using comparativetranscriptome analysis of soybean inoculated with or withoutPhytophthora sojae revealed Glyma.20G241500, a homologof CHI1A in Arabidopsis thaliana, to be induced by P.sojaein soybean. In the present study, we isolated the gene fromsoybean cultivar Nannong 10-1, designating it GmCHI1A. GmCHI1A transcripts were detected in soybean roots, stemsand leaves, with the highest levels in roots. Interestingly,GmCHI1A localizes to the cortical endoplasmic reticulum(ER). GmCHI1A was overexpressed in hairy roots to furtheruncover the roles of CHIs in soybean. Compared to emptyvector-expressing hairy roots (EV) infected with P. sojae,hairy roots overexpressing GmCHI1A (OE) exhibited decreasedP. sojae biomass accumulation, shortened the lesion lengthsand reduced zoospores germination. These results demonstratethat GmCHI1A plays positive roles in the response ofsoybean to P. sojae.

Poster Session : PS 0344 ; Epidemiology and Public Health : Fatty Liver and Economic Development in China

( Jin Zhou Zhu ),( Yu Ming Wang ),( Chao Hui Yu ),( You Ming Li ) 대한내과학회 2014 대한내과학회 추계학술대회 Vol.2014 No.1

Background: Fatty liver is a growing health issue in China. However, the research concerning the infi uence of economic development on the prevalence of fatty liver was sparse. This study is to investigate the relation between economic status and the adult prevalence of fatty liver in the mainland of China. Methods: Literature searches on PubMed, EMBASE, Web of Science and Chinese National Knowledge Infrastructure databases were performed to identify eligible studies published before July 10, 2014. Gross domestic product (GDP) per capita was chosen to assess the economic status. The correlations were obtained by Pearson coeffi cient. Results: 27 studies, from 22 cities and 2 provinces from 1995 to 2013, were included in this study. In general, the prevalence of fatty liver presented a signifi cant correlation with GDP per capita (r = 0. 459, P = 0. 016). To be specifi c, the prevalence in north China correlated with GDP per capita (r = 0. 879, P = 0. 05) and survey year (r = 0. 909, P = 0. 032). In south China, on the contrary, the prevalence failed to correlate with the two indicators. According to the data from multiple years, the city of Shanghai witnessed an ascending prevalence. In terms of gender, the prevalence of female correlated with GDP per capita (r = 0. 572, P = 0. 004) and survey year (r = 0. 483, P = 0. 020). Meanwhile, there was no correlation between the prevalence of male and GDP per capita (P = 0. 129) or survey year (P = 0. 358). Conclusions: In the mainland of China, higher GDP per capita suggested higher adult prevalence of fatty liver, based on the data in the past 20 years. With the development of economy, public health interventions, thus, are essential to prevent the trend of obesity and alcohol abuse.

Two groups of S-Layer proteins, SLP1s and SLP2s, in Bacillus thuringiensis co-exist in the S-Layer and in parasporal inclusions

( Zhou Zhou ),( Dong Hai Peng ),( Jin Shui Zheng ),( Gang Guo ),( Long Jun Tian ),( Zi Niu Yu ),( Ming Sun ) 생화학분자생물학회(구 한국생화학분자생물학회) 2011 BMB Reports Vol.44 No.5

We screened four B. thuringiensis strains whose parasporal inclusions contained the S-layer protein (SLP), and cloned two slp genes from each strain. Phylogenetic analysis indicated these SLPs could be divided into two groups, SLP1s and SLP2s. To confirm whether SLPs were present in the S-layer or as a parasporal inclusion, strains CTC and BMB1152 were chosen for further study. Western blots with isolated S-layer proteins from strains CTC and BMB1152 in the vegetative phase showed that SLP1s and SLP2s were constituents of the S-layer. Immunofluorescence utilizing spore-inclusion mixtures of strains CTC and BMB1152 in the sporulation phase showed that SLP1s and SLP2s were also constituents of parasporal inclusions. When heterogeneously expressed in the crystal negative strain BMB171, four SLPs from strains CTC and BMB1152 could also form parasporal inclusions. This temporal and spatial expression is not an occasional phenomenon but ubiquitous in B. thuringiensis strains. [BMB reports 2011; 44(5): 323-328]

Schisandrin B Improves the Hypothermic Preservation of Celsior Solution in Human Umbilical Cord Mesenchymal Stem Cells

Zhang Ying,Wang Peng,Jin Mei-xian,Zhou Ying-qi,Ye Liang,Zhu Xiao-juan,Li Hui-fang,Zhou Ming,Li Yang,Li Shao,Liang Kang-yan,Wang Yi,Gao Yi,Pan Ming-xin,Zhou Shu-qin,Peng Qing 한국조직공학과 재생의학회 2023 조직공학과 재생의학 Vol.20 No.3

BACKGROUND: Human umbilical cord mesenchymal stem cells (hUCMSCs) have emerged as promising therapy for immune and inflammatory diseases. However, how to maintain the activity and unique properties during cold storage and transportation is one of the key factors affecting the therapeutic efficiency of hUCMSCs. Schisandrin B (SchB) has many functions in cell protection as a natural medicine. In this study, we investigated the protective effects of SchB on the hypothermic preservation of hUCMSCs. METHODS: hUCMSCs were isolated from Wharton’s jelly. Subsequently, hUCMSCs were exposed to cold storage (4 C) and 24-h re-warming. After that, cells viability, surface markers, immunomodulatory effects, reactive oxygen species (ROS), mitochondrial integrity, apoptosis-related and antioxidant proteins expression level were evaluated. RESULTS: SchB significantly alleviated the cells injury and maintained unique properties such as differentiation potential, level of surface markers and immunomodulatory effects of hUCMSCs. The protective effects of SchB on hUCMSCs after hypothermic storage seemed associated with its inhibition of apoptosis and the anti-oxidative stress effect mediated by nuclear factor erythroid 2–related factor 2 signaling. CONCLUSION: These results demonstrate SchB could be used as an agent for hypothermic preservation of hUCMSCs.

3-D Hydrogen-Bonded Frameworks of Two Metal Complexes with Chelidamic Acid: Syntheses, Structures and Magnetism

Zhou, Guo-Wei,Guo, Guo-Cong,Liu, Bin,Wang, Ming-Sheng,Cai, Li-Zhen,Huang, Jin-Shun Korean Chemical Society 2004 Bulletin of the Korean Chemical Society Vol.25 No.5

Complexes M($C_7H_2NO_5)3H_2O{\cdot}H_2O{\cdot}0.25MeCN$ (M=Ni, Co) were crystallized from the reactions of $Ni(CH_3COO)_2{\cdot}4H_2O\;or\;Co(CH_3COO)_2{\cdot}2H_2O$ with KSCN and 2,6-dicarboxy-4-hydroxypyridine (chelidamic acid). The structures were characterized by X-ray crystallography. The crystal structures of 1 and 2 show a distorted octahedral coordination geometry around the M(II) ions, which are chelated by one nitrogen atom and two oxygen atoms of the chelidamic acid and three water molecules. Complexes 1 and 2 display the hydrogen-bonded 3D framework. The magnetic behavior of 2 exhibits antiferromagnetic interaction.

miRNA-218 Inhibits Osteosarcoma Cell Migration and Invasion by Down-regulating of TIAM1, MMP2 and MMP9

Jin, Jie,Cai, Lin,Liu, Zhi-Ming,Zhou, Xue-Song Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.6

Deregulated miRNAs participate in osteosarcoma genesis. In this study, the expression of miRNA-218 in human osteosarcomas, adjacent normal tissues and Saos-2 human osteosarcoma cells was first assessed. Then the precise role of miRNA-218 in osteosarcoma cells was investigated. Upon transfection with a miR-218 expression vector, the proliferation of Saos-2 human osteosarcoma cells determined using the ATPlite assay was significantly suppressed, whilw migration of Saos-2 cells detected by wound healing and invasion determined using transwells were dramatically inhibited. Potential target genes of miR-218 were predicted and T-cell lymphoma invasion and metastasis 1 (TIAM1) and matrix metalloproteinase 2 (MMP2) and 9 (MMP9) were identified. This was confirmed by western blotting, which showed that miR-218 expression inhibited TIAM1, MMP2 and MMP9 protein expression. Collectively, these data suggest that miR-218 acts as a tumor suppressor in osteosarcomas by down-regulating TIAM1, MMP2 and MMP9 expression.

Fluorine-doped porous carbon-decorated Fe₃O₄-FeF₂ composite versus LiNi_0.5Mn_1.5O₄ towards a full battery with robust capability

Ming, Hai,Ming, Jun,Kwak, Won-Jin,Yang, Wenjing,Zhou, Qun,Zheng, Junwei,Sun, Yang-Kook Elsevier 2015 ELECTROCHIMICA ACTA Vol.169 No.-

<P><B>Abstract</B></P> <P>A new fluorine-doped porous carbon-decorated Fe<SUB>3</SUB>O<SUB>4</SUB>-FeF<SUB>2</SUB> composite, referred to as Fe<SUB>3</SUB>O<SUB>4</SUB>-FeF<SUB>2</SUB>@CF<SUB>x</SUB>, was prepared for the first time. The formation mechanism is discussed, and a new concept of introducing double layers of FeF<SUB>2</SUB> and CF<SUB>x</SUB> into the oxide-based anode is presented for lithium ion batteries. Varying the amount of fluorine precursor, derivatives of Fe<SUB>3</SUB>O<SUB>4</SUB>@CF<SUB>x</SUB> and FeF<SUB>2</SUB>@CF<SUB>x</SUB> were further obtained, allowing an original analysis of their electrochemical behaviors. As-prepared Fe<SUB>3</SUB>O<SUB>4</SUB>-FeF<SUB>2</SUB>@CF<SUB>x</SUB> can deliver a high capacity of 718mAhg<SUP>−1</SUP> at 50mAg<SUP>−1</SUP>. Under a hash rate of 1600mAg<SUP>−1</SUP>, the capacity of Fe<SUB>3</SUB>O<SUB>4</SUB>-FeF<SUB>2</SUB>@CF<SUB>x</SUB> (around 338mAhg<SUP>−1</SUP>) is higher than that (200mAhg<SUP>−1</SUP>) of FeF<SUB>2</SUB>@CF<SUB>x</SUB>. Further, its capacity retention of 97% over 100 cycles is much better than the 59.4% observed for Fe<SUB>3</SUB>O<SUB>4</SUB>@CF<SUB>x</SUB>. The positive effect of the CF<SUB>x</SUB> layer on the electronic conductivity and ionic diffusion ability was confirmed. The role of FeF<SUB>2</SUB> in the stabilization of the structure of CF<SUB>x</SUB> and Fe<SUB>3</SUB>O<SUB>4</SUB> is also discussed. Further, a new battery composed of Fe<SUB>3</SUB>O<SUB>4</SUB>-FeF<SUB>2</SUB>@CF<SUB>x</SUB>/LiNi<SUB>0.5</SUB>Mn<SUB>1.5</SUB>O<SUB>4</SUB> with a robust rate capability was assembled and delivered a reversible capacity of 565mAhg<SUP>−1</SUP> (<I>vs.</I> anode) at 100mAg<SUP>−1</SUP> with a high potential of 3.3V and a capacity retention of 81.5% over 50 cycles.</P> <P><B>Highlights</B></P> <P> <UL> <LI> A new anode of fluorine-doped porous Fe<SUB>3</SUB>O<SUB>4</SUB>-FeF<SUB>2</SUB>@CF<SUB>x</SUB> composite is readily prepared. </LI> <LI> The CF<SUB>x</SUB> layer enhances the conductivity of Fe<SUB>3</SUB>O<SUB>4</SUB> and ensures a fast Li<SUP>+</SUP> diffusion. </LI> <LI> The FeF<SUB>2</SUB> can stabilize the structure of Fe<SUB>3</SUB>O<SUB>4</SUB> during the (dis) charge process. </LI> <LI> The Fe<SUB>3</SUB>O<SUB>4</SUB>-FeF<SUB>2</SUB>@CF<SUB>x</SUB> can deliver a high capacity with a robust rate capability. </LI> <LI> A full cell of Fe<SUB>3</SUB>O<SUB>4</SUB>-FeF<SUB>2</SUB>@CF<SUB>x</SUB>/LiNi<SUB>0.5</SUB>Mn<SUB>1.5</SUB>O<SUB>4</SUB> with high performance is assembled. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>A new anode of fluorine doped porous Fe<SUB>3</SUB>O<SUB>4</SUB>-FeF<SUB>2</SUB>@CF<SUB>x</SUB> composite with double layers of FeF<SUB>2</SUB> and CF<SUB>x</SUB> was presented for the first time, and a high rate capability was obtained in lithium ion battery. Besides, a new full battery of Fe<SUB>3</SUB>O<SUB>4</SUB>-FeF<SUB>2</SUB>@CF<SUB>x</SUB>/LiNi<SUB>0.5</SUB>Mn<SUB>1.5</SUB>O<SUB>4</SUB> with a high capacity of 565mAhg<SUP>−1</SUP> (<I>vs</I>. anode) at the current density of 100mAg<SUP>−1</SUP> was successfully introduced. It demonstrated a robust rate capability, high operating potential of 3.3V and fine cycle ability over 50 cycles with capacity retention of 81.5%.</P> <P>[DISPLAY OMISSION]</P>

Clinical Significance of Expression and Amplification of the DcR3 Gene in Pancreatic Carcinomas

Zhou, Jian,Song, Shi-Duo,Li, De-Chun,Zhou, Jin,Zhu, Dong-Ming,Zheng, Shi-Ying Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.2

This study aimed to investigate the clinical significance of expression and amplification of decoy receptor 3 (DcR3) in pancreatic carcinomas (PC). mRNA expression was detected by PQ-PCR, and amplification was determined. DcR3 protein expression was detected by immunohistochemistry and ELISA. Correlations between DcR3 expression and clinical pathological factors were analyzed. The relative amount of DcR3 in PC tissues and non-cancerous tissues showed a statistically significant difference, 21 cases displaying more than two fold DcR3 amplification, while no such amplification was found in normal pancreatic tissues. DcR3 positive cell staining was located in the cytoplasm. The positive rate of DcR3 in PC and non-cancerous tissues showed a significant difference. DcR3 mRNA expression was correlated with clinical staging, size of the tumor, lymph node metastasis and histological staging, while protein expression was correlated with clinical data like tumor size. DcR3 gene amplification only correlated with tumor size. The level of DcR3 in serum of the PC resectable group before operation was $72.2{\pm}10.2$ pg/ml, showing a significant difference compared to gallbladder carcinoma group (GC) or pancreatic benign tumor (PBT) group (P < 0.01). In conclusion, DcR3 amplification is correlated with DcR3 expression in PC tissues, especially those clinical pathological factors which reflect tumor progression. Assessment of DcR3 level in sera of PC patients may be helpful for the early diagnosis and prognostic judgement.

Synthesis and Characterization of Folic Acid Labeled Upconversion Fluorescent Nanoprobes for in vitro Cancer Cells Targeted Imaging

Ming Huang,Lijun Wang,Xiaojuan Zhang,Jin Zhou,Lihua Liu,Yuefang Pan,Bin Yu,ZHANGSEN YU 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2017 NANO Vol.12 No.5

Upconversion nanoparticles (UCNPs) are widely used in the field of biomedicine, such as biosensing, cell labeling and medical multimodal imaging because of their unique optical properties. In this paper, we demonstrated the synthesis of polyethylenimine-modified NaLuF4:Yb,Er (RE = Lu 0.78, Yb 0.18, Er 0.02) UCNPs in three different solvents, such as water, ethylene glycol and diethylene glycol. The as-prepared UCNPs were characterized and the experimental results showed that the UCNPs synthesized in ethylene glycol had excellent properties. The obtained UCNPs in ethylene glycol had the smallest particle size and uniform size distribution, and the pure cubic phase of crystallization and Dynamic light scattering and particle dispersion index (DLS/Pdi) were the smallest. What's more, the upconversion fluorescence intensity was 7 and 52 times greater than that of UCNPs synthesized in diethylene glycol and water, respectively. In addition, the factors of reaction solvent that had an impact on the particle size, morphology, crystalline phase, DLS and upconversion fluorescence intensity of the synthesized UCNPs were discussed. Moreover, in order to obtain the targeted nanoprobe, we used an EDC/NHS covalent coupling method to modify folic acid to the NaLuF4:Yb,Er/PEI UCNP surface. The NaLuF4:Yb, Er/PEI–FA upconversion fluorescent nanoprobes had low cytotoxicity and were suitable for the application in HeLa cells targeted fluorescent imaging.

Extraction of hyper-elastic material parameters using BLSTM neural network from instrumented indentation

Jing Jin Shen,Jia Ming Zhou,Shan Lu,Yue Yang Hou,Rong Qing Xu 대한기계학회 2023 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.37 No.12

Instrumented indentation is a versatile method of extracting hyper-elastic material parameters, particularly useful for applications where stress-strain data are difficult to be insitu measured. Because the analytical force-displacement relation is still unavailable for the indentation of hyper-elastic materials, identifying hyper-elastic parameters often requires an iterative optimization strategy that fits finite element simulations with experimental data. However, the optimization strategy is burdened by heavy computation and its prediction accuracy is greatly influenced by the choice of optimization algorithm. To address these challenges in this study, a bidirectional long short-term memory (BLSTM) neural network is presented that directly predicts hyper-elastic material parameters from indentation load-displacement data, focusing on Mooney-Rivlin hyper-elasticity as an example. To improve the predication accuracy, the condition numbers for the inverse identification of the hyper-elastic parameters are investigated. And, a normalization procedure is proposed to treat the input data, which can guarantee the BLSTM network is well-conditioned. During evaluation, the trained BLSTM network significantly outperforms the iterative optimization strategy using a genetic algorithm. Furthermore, the effect of the normalization procedure is demonstrated.