청가시덩굴 추출물의 기능성 원료 표준화를 위한 지표성분 Resveratrol, trans-Scirpusin A의 분석법 개발 및 검증

권진관(Jin Gwan Kwon),정연우(Yeon Woo Jung),최윤혁(Yun-Hyeok Choi),이지은(Ji Eun Lee),정원식(Wonsik Jeong),이정아(Jung A Lee),최춘환(Chun Whan Choi),안은경(Eun-Kyung Ahn),최용문(Yongmun Choi),홍성수(Seong Su Hong) 한국식품영양과학회 2022 한국식품영양과학회지 Vol.51 No.11

본 연구는 HPLC를 이용하여 청가시덩굴 추출물을 개별인정형 건강기능식품의 기능성 원료로 개발하기 위한 원료 표준화의 일환으로, 청가시덩굴 추출물의 지표성분을 resveratrol과 trans-scirpusin A로 설정하고 이에 대한 HPLC 분석법을 확립하여 유효성의 검증을 실시하였다. 분석법 유효성 검증은 특이성, 직선성, 정확도, 정밀도, 검출한계 및 정량한계 등을 통해 분석법의 신뢰성을 검증하였으며, 그 결과 표준용액과 청가시덩굴 추출물 간의 HPLC 크로마토그램 및 UV spectrum의 일치 여부 등의 비교를 통해 다른 물질과 간섭 없이 피크가 분리된 것으로 특이성을 확인하였다. 또한 표준용액 검량선의 상관계수(R²)는 0.9999로 매우 우수한 직선성으로 관찰되어 분석에 적합한 것으로 확인되었으며, 검량선의 기울기 및 표준편차를 이용한 검출한계는 resveratrol이 0.98 μg/mL, trans-scirpusin A는 0.49 μg/mL였고 정량한계는 resveratrol이 2.98 μg/mL, trans-scirpusin A는 1.48 μg/mL로 각각 확인되었다. 청가시덩굴 추출물에 표준물질을 3개 농도 첨가하고 분석한 회수율은 resveratrol이 98.77~99.24%, trans-scirpusin A는 98.45~99.45%로 나타나 정확성이 있는 것을 확인할 수 있었다. 청가시덩굴 추출물의 조제 농도 2.2, 4.4 및 6.6 mg/mL에서 반복성은 resveratrol이 0.99~1.22%, trans-scirpusin A는 1.12~1.32%를, 실험실 내 정밀성에서는 일내 정밀성은 resveratrol이 0.67~0.87%, trans-scirpusin A는 1.18~1.33%로 나타났고 일간 정밀성은 resveratrol이 0.93~1.22%, trans-scirpusin A는 1.33~2.27%로 확인되어 본 분석법은 정밀성이 있음을 확인할 수 있었다. 이상의 분석결과를 통해 확립된 청가시덩굴 추출물의 지표성분인 resveratrol과 trans-scirpusin A의 HPLC 분석법은 적합한 시험법으로 검증되었으며, 본 시험법은 향후 청가시덩굴 추출물의 건강기능식품 기능성 원료 개발과 표준화를 위한 기초자료로 활용될 것으로 사료된다. This study was undertaken to establish an analytical method using high-performance liquid chromatography (HPLC). HPLC for the standard determination of resveratrol and trans-scirpusin A as functional ingredients in Smilax sieboldii extract. We evaluated the specificity, linearity, accuracy, precision, limit of detection (LOD), and limit of quantitation (LOQ) of various analytical methods for detecting resveratrol and trans-scirpusin A using HPLC. The specificity was confirmed by the chromatogram obtained using the HPLC analytical method. Also, the results of UV and the coefficient of correlation (R²) obtained was 0.999, which confirmed that this was a suitable analysis with high linearity. The LOD was 0.98, 0.49 μg/mL, and LOQ was 2.98, 1.48 μg/mL, which was confirmed as a suitable limit level for the analysis of resveratrol and trans-scirpusin A content in the S. sieboldii extract. The recovery of resveratrol and trans-scirpusin A content was determined to be 98.77±0.73∼99.24±1.47% and 98.45±1.18∼99.45±1.66%, respectively, indicating high accuracy. The intra-day repeatability and the intra-laboratory precision of the daily repetition were confirmed to be 0.67∼0.87%, 1.18∼1.33% and 0.93∼1.22%, 1.33∼2.27%, respectively, for trans-scirpusin A, for the relative standard deviation. These results indicate that the reported HPLC method is simple, reliable, and reproducible for the detection of resveratrol and trans-scirpusin A in S. sieboldii extract.

청소년 우울증 측정 및 관리를 위한 스마트폰 어플리케이션 콘텐츠 개발

박정선(Park, Jung-Sun),조재숙(Cho, Jae-Suk),함선희(Ham, Sun-Hee),박지수(Park, Ji-Soo),권정아(Gwon, Jung-A) 한국웰니스학회 2016 한국웰니스학회지 Vol.11 No.2

본 연구는 청소년 정신건강의 핵심문제 중 하나인 우울증의 측정과 관리를 위한 스마트폰 어플리케이션 콘텐츠 개발을 목적으로 하며, 이는 충남 소재 A대학의 청소년상담 관련학과, 컴퓨터공학 관련학과의 교수와 학생들의 협업으로 이루어졌다. 사용대상은 우울증의 예방 및 관리를 위해 집단상담에 참여하는 청소년들이며, 개발된 어플리케이션은 집단상담을 돕기 위해 사용될 예정이다. 콘텐츠 구성을 위해 먼저, 청소년 우울증의 발생원인과 특성, 수준측정방안, 심리치료이론을 고찰하였다. 다음으로 기존에 개발, 발표된 국내·외 어플리케이션과 선행연구를 통해 콘텐츠 내용과 실용성 등을 비교분석하였다. 이를 통해 청소년 우울증수준 측정방법으로 BDI-Ⅱ(우울척도), 심리치료 기법으로 인지행동치료, 치료 촉진방략으로 집단상담을 선정했다. 따라서 본 연구는 이에 초점을 맞춰 콘텐츠를 구성 및 개발하였으며, 개발 목적 및 실용화의 적절성 파악을 위해 청소년 5인을 대상으로 예비조사를 실시하였다. 예비조사 결과에서 제시된 제안점은 전문가와 실제 사용대상의 의견을 수렴하여 수정 보완할 예정이다. 결론적으로 본 콘텐츠는 일상성을 바탕으로 한 능동적 정서관리를 통해 효율적으로 청소년의 정신건강 웰니스를 지원할 것이다. The present study aims to develop the digital content of a smart-phone app for the measurement and management of depression – a key issue in adolescents’ mental health. This app has been developed by a psychology laboratory and a computer software engineering laboratory for an interdisciplinary convergence study. The target users of the app are adolescents who participate in group counseling for prevention and management for depression. The app is planned to be used as a tool for the group counseling. To develop the app content, we conducted a literature review. We then comparatively analyzed the key aspects of existing domestic and foreign apps designed to help for depression. The results of the analysis suggested the Beck Depression Inventory-II for the assessment method, the cognitive behavioral approach for the psychotherapeutic approach, and the group counseling that allows mutual support. Therefore, in this study, we organized and developed the app content by focusing on the identified aspects and conducted a pilot test with five adolescents to determine if the app met the development objectives. Modifications and improvements will be made based on the suggestions obtained from the pilot test and expert reviews. In conclusion, this app content is expected to enhance the wellness of adolescents effectively by improving proactive emotional management on a daily basis.

한국인에서 5,10-Methylenetetrahydrofolate Reductase 유전자 다형성 분석 및 임신의 결과에 미치는 영향

김아리 ( Ari Kim ),강은지 ( Eun Ji Kang ),이경아 ( Kyung A Lee ),박세나 ( Se Na Park ),박종순 ( Jong Soon Park ),박보현 ( Bo Hyun Park ),박혜숙 ( Hyesook Park ),박미혜 ( Mi Hye Park ),전선희 ( Sun Hee Chun ),안정자 ( Jung Ja Ahn ) 대한주산의학회 2006 大韓周産醫學會雜誌 Vol.17 No.3

목적: 본 연구는 한국인 임신 여성에서 MTHFR 유전자형 변이와 혈청내 호모시스테인 및 엽산의 농도와 이들이 임신에 미치는 영향을 알아보고자 하였다. 방법: 임신부 600명에게서 혈액을 채취하여 얻어진 DNA로 MTHFR C677T와 A1298C 유전자의 다형성을 PCR-RELP로 분석하였고 혈청내 호모시스테인은 HPLC 방법으로, 엽산은 RIA 방법으로 측정하였다. 결과: 혈청내 호모시스테인은 MTHFR C677T 유전자형이 C/C형이나 C/T인 군에 비해 T/T인 군에서 유의하게 높았으며 MTHFR A1298C 유전자형이 A/C형이나 C/C인 군에 비해 A/A인 군에서 역시 유의하게 높았다( p<0.05). 혈청 호모시스테인은 모든 MTHFR 유전자형에서 혈청 엽산농도와 역상관관계를 보였으나, 변이형 유전자형(T/T와 A/A유전자형)에서 더 큰 역 상관관계를 보였다. 15?mol/L 이상의 고호모시스테인혈증의 임신부에서 임신 주수와 출생체중이 36.1주, 3053.8 g으로 15 ?mol/L미만인 군의 38.3 주, 3215.3 g 보다 유의하게 낮았다( p<0.05). 결론: 혈청 호모시스테인의 농도는 MTHFR C677T와 A1298T 유전자의 다형성에 의해 영향을 받으며, MTHFR C677T와 A1298T 유전자의 다형성과 혈청 호모시스테인은 모두 임신주수와 신생아의 체중에 영향을 미친다. Objective: The purpose of this study was to analyze MTHFR polymorphism among the Korean population and to evaluate the relationship between serum levels of homocysteine and MTHFR polymorphism and also to investigate the effect on pregnancy outcomes. Methods: DNA was extracted from whole blood of 600 pregnant women. All samples were genotyped for the C677T and A1298C polymorphisms in MTHFR gene by PCR-RELP assay. Serum levels of homocysteine and folate were measured by high performance liquid chromatography for homocysteine and radioassay for folate. Pregnancy outcomes were estimated by gestational weeks and birth weights of newborns. Results: Serum homocysteine was higher in women with the T/T genotype than those with the C/T or C/C genotype of the MTHFR C677T polymorphism (p<0.05). And also serum homocysteine was higher in women with the A/A genotype than those with the A/C or C/C genotype of the MTHFR A1298C polymorphism (p<0.05). Serum homocysteine was negatively correlated with serum folate in all MTHFR genotypes, especially prominent in T/T genotype of MTHFR C677T polymorphism and A/A genotype of MTHFR A1298C polymorphism. Gestational age and the birth weight of infant from hyperhomocysteinemic mothers whose homocysteine levels higher than 15 μmol/L were 36.1 weeks, 3053.8 g, respectively, which were significant lower than those from normohomocysteinemic mothers (38.3 weeks, 3,215.3 g) (p<0.05). Conclusion: Serum homocysteine was influenced significantly by MTHFR C677T polymorphism and MTHFR A1298C polymorphism. MTHFR C677T and A1298C polymorphism and serum homocysteine levels affect pregnancy outcomes, although not mainly by serum folate level.

기억력 저하를 호소하는 노인들을 대상으로 INM176를 3개월간 임상시험한 후의 치료성적 및 부작용 평가 보고

김지혜,고선규,고효정,권영아,김성환,김재경,김태은,박재우,서민영,송영란,이인수,김도관 大韓神經精神醫學會 2003 신경정신의학 Vol.42 No.2

Objectives : We examined the effects of INM 176 (K-l107) compared with placebo on the cognitive functions of 92 old aged subjects with cognitive impairment. Methods : This was a prospective, 12 week, double-blind, Placebo-controlled clinical trial. The elderly who achieved a score of less than 25 points on the K-MMSE or showed a high risk of Alzheimer's disease from the 7-Minute Neurocognitive Screening Battery were considered to have objective impairment and were selected as subjects for this study. The subjects were randomized to placebo or INM 176 group. The outcome measures were from the Alzheimer's Disease Assessment Scale-cognitive subscale (ADAS-Cog), the Instrumental Activities ofDaily Living (IADL) and the Korean Geriatric Depression Scale (KGDS) and two kinds of computerized priming tests. After setting the total error score in the Alzheimer's Disease Assessment Scale-Cognitive section (ADAS-cog) as the repeated measurement factor, an analysis of variance of the combined factor design was done between the placebo and INM 176 group. Results : The interaction effect of time (pre- and post-trial) and group (placebo and INM 176 group) was significant in the analysis of the ADAS-cog's total error score. The INM 176 group's total error score in the ADAS-cog decreased significantly (p<0.01), whereas the placebo group showed a slight increase. The mean changes in IADL and GDS from baseline scores favored in the INM 176 group than in the placebo group. Outcome changes ofADAS-cog, IADL, KGDS scores during the 12 week clinical trail ofINM 176 and placebo demonstrated favorable responses in the INM176 administered group. Conclusions : This is a preliminary clinical trial result of INM176 as a memory pill. Based on these results, INM176 may be a candidate molecule for the improvement of cognitive functions, including memory, Further clinical trial should demonstrate its efficacy.

식품영양학 전공자를 위한 인턴십 교육 프로그램 개선방안

주나미,윤지영,김옥선,정현아,박상현,고영주,조기원 한국식생활문화학회 2005 韓國食生活文化學會誌 Vol.20 No.1

This study was conducted to evaluate the effect of current dietetic internship program. The survey sample was dietetic practitioners who were charged with a training for dietetic students. The questionnaire requested information about demographic information, experience of guidance for the dietetic student, self evaluation as trainer, fortification items in university education, and difficulties of the internship program. Data was analyzed using SAS for windows(version 6.12), Descriptive statistics were used such as means. standard deviations. frequency distribution and percentages. Comparison of mean differences was analyzed using t-test and ANOVA. Participants identified how effective the current internship program was. Result indicated that the effectiveness was not high(2.81), and under 29 years old age group, under 6 years work experience group. and contracted foodservice group had positive opinion of the program significantly. Under 29 years old age group, evaluated themselves as a good trainer. The results revealed that respondents wanted to be fortified the practical training courses in school curriculum.

석회유담즙증 1예

이지아,김도영,정혜경,문일환,신수연,곽재진 梨花女子大學校 醫科大學 醫科學硏究所 2003 EMJ (Ewha medical journal) Vol.26 No.1

Milk of calcium bile or limy bile is a rare disorder in which the gallbladder lumen is filled with a thick, paste-like, radio-opaque material. Churchman first reported a case of curious deposition of calcium salts within the gallbladder in 1911. Volkmann first used the term Kalkmilchgalle or "milk of calcium bile" in 1926. The radio-opaque material is almost entirely composed of calcium carbonate by weight. The mechanisms by which bile turns limy and radio-opaque or the time required for this biochemical alteration to occur are not well understood. The limy bile is found mostly in a gallbladder complicated by cholecystitis. Recently, we experienced a 33 year old woman who had a milk of calcium bile in the gallbladder lumen. We report this case with a review of literature.

Quality Characteristics of Jeju Beets Treated by Various Drying Conditions

A-Ra Ko,Hee-Jung Jin,Ji-Hyun Im,Jung-Hyun Nam,Ji-Yeon Chun 한국식품영양과학회 2021 한국식품영양과학회 학술대회발표집 Vol.2021 No.10

Beets (Beta vulgaris) contain a lot of betalains, a type of natural pigment, so they are known to be red and have strong antioxidant abilities. This study was conducted to compare the physicochemical properties of Jeju beets processed by hot air drying and combined drying. Beets were sliced to 3 mm and dried by hot air drying (13 hours at 50°C, 6 hours at 60°C, or 4.5 hours at 70°C) or combined drying (hot air and infrared radiation). Appearance, water activity, moisture content, pH, color, rehydration ratio (RR), total polyphenol content (TPC), FRAP value, DPPH radical scavenging ability, and ABTS radical scavenging ability were determined. In hot air drying, as the drying temperature increased, water activity, moisture content, and b* value were decreased while L*, a* value, RR, TPC, FRAP value, and ABTS radical scavenging ability were increased. The combined drying had lower water activity and pH value; moreover, it had a higher L*, a* value, RR, TPC, FRAP value, DPPH radical scavenging ability, and ABTS radical scavenging ability. In this study, it was found that the combined drying was more suitable than hot air drying to maintain the antioxidant ability of beet.

음식물 폐기물류로 제조된 퇴비의 고형연료 가치 평가 연구

이경섭,장한별,정은지,도아름,하진욱 순천향대학교 부설 산업기술연구소 2021 순천향 산업기술연구소논문집 Vol.27 No.2

In 2019, the daily generation of food waste in Korea was 14,314 tons, which is a steady increase compared to the past. To efficiently use food waste as an effective resource, composting and feeding are carried out. In Korea, attention is gradually focused on a sustainable resource circulation society. Compost made of food waste has problems with foreign substances, heavy metals, and salt content, and solid fuelization research is being actively conducted to compensate for this. In this study, infrared spectroscopy(FT-IR), organic content measurement(TGA), and chemical composition were measured to compare the fuel characteristics with wood pellets, which are major solid fuels, in order to examine the physical and chemical properties of food waste compost.

유방암의 위와 대장전이

유현아,김은영,서민지,정은,조민정,오현진,장지혜,박지찬,이정의,박석영 이화여자대학교 의과학연구소 2014 EMJ (Ewha medical journal) Vol.37 No.2

Gastric metastasis from breast cancer is rare and only six cases have been reported in Korea. Colon metastasis is more rare than gastric metastasis. We report a 63-year-old woman with gastric and colon metastases of invasive lobular carcinoma of breast. She was diagnosed as right breast cancer, received right modified radical mastectomy 10 years ago and has been treated with chemotherapy and hormone therapy. Investigating for melena and a small caliber of stool, we found gastric and colon metastases. The diagnosis of metastatic breast cancer was made through gross pathologic and immunohistochemistry staining. We report a case with gastric and colon metastases from breast cancer and a review of the associated six case reports in Korea.

열풍 및 복합건조된 제주산 비트의 이화학적 특성과 항산화 활성

고아라(A-Ra Ko),남정현(Jung-Hyun Nam),진희정(Hee-Jung Jin),임지현(Ji-Hyun Im),김한상(Han-Sang Kim),천지연(Ji-Yeon Chun) 한국식품영양과학회 2022 한국식품영양과학회지 Vol.51 No.6

본 연구에서는 기존 비트의 한계점을 극복하고 고품질의 건조 비트를 제조하기 위한 기초 자료를 제시하고자 제주산 비트를 다양한 온도 조건에서 열풍 및 적외선 복합건조 하였고, 이에 따른 품질변화를 비교하여 건조 조건이 비트의 이화학적 특성과 항산화 활성에 미치는 영향에 대해서 조사하였다. 3 ㎜ 두께로 비트를 절단한 후 70℃에서 4.5시간, 60℃에서 6시간, 50℃에서 13시간 열풍건조 하거나 100℃에서 1시간 복합건조 하였다. 열풍건조한 비트는 건조 온도가 높아질수록 수분함량, 수분활성도, b<SUP>*</SUP>값(황색도), betanin 함량이 감소했고 건조수율과 pH에는 변화가 없었으며, L<SUP>*</SUP> 값(명도), a<SUP>*</SUP> 값(적색도), ΔE 값(건조 전 원물과의 색차), 재복원율, vulgaxanthin-Ⅰ 함량, 총 폴리페놀 함량, DPPH 라디칼 소거능, ABTS⁺ 라디칼 소거능, FRAP 활성은 증가하는 경향을 보였다. 그중에서 수분활성도, a<SUP>*</SUP>값, ΔE 값, FRAP 활성은 건조 온도가 높아짐에 따라 유의적으로 변화하였다. 복합건조한 비트는 열풍건조한 비트보다 L<SUP>*</SUP>값, a<SUP>*</SUP> 값, ΔE 값, 재복원율, 총 폴리페놀 함량, DPPH 라디칼 소거능, ABTS⁺ 라디칼 소거능, FRAP 활성이 유의적으로 높았고 pH는 유의적으로 낮았으며, 수분함량, 수분활성도, b<SUP>*</SUP>값, betanin 함량, vulgaxanthin-Ⅰ 함량에서는 열풍건조한 비트와 유의적인 차이를 나타내지 않았다. 이상의 결과에 따르면 복합건조한 비트가 다른 조건에서 건조를 진행한 비트에 비해 소요되는 시간이 매우 짧고 pH가 낮으며, 재복원율, 총 폴리페놀 함량, 항산화 활성이 높아 고품질의 건조 비트를 제조하기 위해서는 열풍뿐만이 아니라 적외선도 적절히 병용하는 것이 적합할 것으로 보인다. Beet (Beta vulgaris L.) is a healthy food that has recently attracted the consumer’s attention. However, beet has low storage stability due to the high moisture content. Therefore, a drying process is necessary to improve the beet quality. In this study, Jeju beet was dried by applying two processes: hot air drying (4.5 hours at 70℃, 6 hours at 60℃, 13 hours at 50℃) and combined drying (hot air and infrared radiation, 1 hour at 100℃). The effect of both processes was investigated on the physicochemical properties and antioxidant activity was investigated. The dried beets were compared for their appearance, drying yield, moisture content, water activity, pH value, color, rehydration ratio, betalain pigment content, total polyphenol content, DPPH radical scavenging activity, ABTS⁺ radical scavenging activity, and FRAP assay. The beet subjected to combined drying was determined to have high storage properties due to its low moisture content and pH value. In addition, the combined dried beet showed the highest rehydration ratio and antioxidant activity. Taken together, our results indicate that an appropriate combination of hot air and infrared radiation is suitable to produce high-quality dried beet.