심근경색 모델 흰쥐에서 스트레스가 B-type Natriuretic Peptide 발현 및 심박변이율 변화에 미치는 영향

이삼윤(Sam Youn Lee),이미경(Mi Kyoung Lee),김남호(Nam-ho Kim),주민철(Min Cheol Joo),조항정(Hyang Jeong Jo),강지숙(Ji Sook Kang),김병숙(Byung Sook Kim),윤선식(Sun Sik Yoon),최을식(Eul Sig Choi),이문영(Moon Young Lee) 대한스트레스학회 2010 스트레스硏究 Vol.18 No.3

심박변이율의 감소는 심근 경색 환자에서 예후의 악화와 관련되어 있다. 심박변이율 중 very low frequency가 심부전환자의 예후를 판단할 수 있는 독립적 인자로서 사용이 가능하다고 보고된 바 있으며, 심실의 압력 증가에 반응해서 생성되는 B-type natriuretic peptide가 심근 경색의 예후 인자로서 사용할 수 있음 역시 보고된 바 있다. 본 연구에서는 심근경색 모델을 제작하여 일정의 회복 기간을 거친 다음 다시 일정 기간의 스트레스를 겪게 한 후 심박변이율의 변화 및 심실 조직에서의 BNP 발현 정도를 비교하여 스트레스에 의한 영향을 관찰하고자 하였다. Sprague-Dawley계 수컷 흰쥐 15마리를 대상으로 하였다. 실험동물은 인위적 스트레스 및 수술을 받지 않은 대조군(CON, n=5), 심근경색 수술을 받은 후 restraint stress를 받지 않은 군(MI+No Stress, n=5), 심근경색 수술을 받은 후 1주일 동안 restraint stress를 받은 군(MI+Stress, n=5)으로 분류하였다. 심근경색 모델 제작 직후 15분 이상 심전도의 변화를 기록하였으며, 2개월 정도 후 심근경색 수술을 받은 동물을 두 군으로 나누어 그 중 한 군에는 1주일 동안 restraint stress를 가한 후 restraint stress를 가하지 않은 동물과 심박변이율을 비교 분석하였다. 심실 조직은 좌심실 전벽 부위의 위축을 관찰할 수 있었고, 대식세포에 의하여 응고, 괴사된 심근세포의 탐식과 혈관이 풍부한 육아조직 및 섬유 변화를 볼 수 있었다. 심박변이율은 심근경색 수술을 받은 직후 심박수는 유의한 증가를 보였고, standard deviation of the normal to normal intervals (SDNN), very low frequency (VLF) 및 low frequency (LF)의 유의한 감소를 보였다. 심근경색 수술을 받았던 동물에서 두 달 정도의 회복 기간을 거친 후 스트레스를 받지 않은 군은 심박수 및 기타 심박변이율 분석에서 SDNN 값을 제외하고는 정상군과 유의한 차이를 관찰할 수 없었던 반면 1주일 동안 스트레스를 받은 군에서는 심박수가 다시 유의하게 증가했을 뿐만 아니라 SDNN, VLF 및 LF 역시 정상군과 유의한 차이를 보였다. 이상의 결과를 종합하면 심근경색 동물에 대해 1주일 동안 스트레스를 가한 결과 심박변이율이 감소하고 심실에서의 BNP 발현은 더욱 증가하여 악화함을 보여주고 있다. Diminished heart rate variability (HRV) is associated with less favorable prognosis after myocardial infarction (MI). It has been reported that very low frequency (VLF) power in HRV analysis is an independent risk predictor in patients with congestive heart failure and B-type natriuretic peptide (BNP) can be used as a prognostic factor of MI. The purpose of this study was to investigate whether stress has an effect to the changes of BNP expression and/or heart rate variability in MI model in rats. Male Sprague-Dawley rats were divided into 3 groups: control group (CON), MI group (MI+No Stress), and MI followed by stress group (MI+Stress). MI+Stress group rats were raised for a two month recovery period after the operation, followed by being exposed to restraint stress for 2 hours per day for 1 week. Electrocardiogram was recorded after the operation and the last day after 1 week of stress. The frequency components of HRV were calculated in the frequency domain such as VLF, low frequency (LF), high frequency (HF) and so on. In HRV analysis, standard deviation of the normal to normal intervals (SDNN) was significantly reduced in both groups compared to the control group. VLF and LF also were significantly reduced in MI+Stress group compared to the control groups. In addition, BNP expression in western blotting was shown the strongest bands in MI+ Stress group among experimental groups. These results suggest that BNP and HRV were aggravated by stress in MI rat model. (Korean J Str Res 2010;18:275∼285)

동진강 수계 농업용수 수질평가

한수곤,최정식,문영훈,엄미정,김갑철 한국환경농학회 2000 한국환경농학회지 Vol.19 No.2

동진강 수계의 수질현황을 파악하기 1994∼1998년 까지 4∼9월에 월 1회에 걸쳐 5개년간 조사한 결과를 요약하면 다음과 같다. 월별로는 빗물에 의한 희석효과로 7, 8월에 수질이 가장 양호하였으며, 4월이 다소 불량하였으나 수도생육 피해 한계농도에는 미치지 못하는 수준이었다. 지점별 수질현황은 생활하수 유입지인 정읍천과 지천의 폭이 좁은 원평천의 무기성분 함량이 높았으며 동진천 상류지역에서 가장 양호하였다. 연도별 현황은 '94년 이후 '95년까지는 수질이 악화되는 경향을 보였으나 '95년 이후로는 각 성분 함량이 낮았지는 등 점점 양호해지고 있으며 COD함량으로 볼 때 특히, 정읍천의 수질이 개선되고 있음을 알 수 있었다. 양이온과 음이온의 총당량온 4월에 가장 높았고 7, 8월에 가장 낮았으며 양이온/음이온의 당량비는 5월에 높았고 7월이 가장 낮았으며 지점에 따라서는 총당량은 정읍천, 원평천, 동진천 하류, 중류, 상류의 순위였으며 양이온/음이온 총당량 비율은 원평천이 상대적으로 작은 수치를 보였지만 지역간 큰 차이는 보이지 않았다. 수질 중 각 성분 상호간의 상관관계를 보면, 대개의 성분 상호간에 유의성이 높게 나타났으나, NO₄^--N는 대개의 성분과 유의성이 없었고, PO₄^(3-)-P도 COD, NH₄^+-N, NO₃^--N, SO₄^(2-)과는 유의성을 보이지 않았다. This study was conducted to monitor the irrigation water qualities along Dong-Jin river watershed. The water quality was surveyed at 6 sites from April to september during 1994∼1998. And the results were as follows : In July and August, water quality was better than that of any other months due to dilution with rainwater. Whereas, it became worse in April but it involved lower contents than limitted contents affected to the crop damage. Content of inorganic components was higher at Jeong-up and Won-pyeong stream. The reason for it that Jeong-up stream was deteriorated with sewage water from Jeong-up city, and Won-pyeong stream has narrow width. Water quality in upstream of Dong-Jin river, was evaluated best conditions in all sampling sites. For investigated period, water quality got worse from 1994 to 1995 but it was getting better to 1998 after 1995, especially at Jeong-up stream. The total equivalent of cation and anion was the highest at April through all months and at Jeong-up stream in sampling sites. Equivalent ratio of cation to anions(∑C/∑A) was higher at May than any other months and lower at Won-pyeong streams than any other sites. The value of most inorganic components was highly correlated with those of other components. But the value of NO₃^--N was not correlated with that of most components, and PO₄^(3-)-P was not correlated with COD, NH₄^+-N, NO₃^--N, SO₄^(2-_.

응급실에 내원한 환자에서 어지럼증의 원인과 임상양상의 분석

문원식,정경운,위준선,문정미,전병조,김용권,소정일,류진호,허탁,민용일 대한응급의학회 2001 대한응급의학회지 Vol.12 No.3

Background: Recently, The number of patients who have been complaining of the vertigo or dizziness has been increasing due to rapid growth of elderly population and senile disease. The differentiation of dizzy patients is not familiar to most emergency physicians. This study was designed to differentiate true vertigo and to investigate the clinical difference among central vertigo, peripheral vertigo, and other causes of dizziness. Methods: The authors analyzed the cases of 237 dizzy patients who visited the emergency department of Chonnam University Hospital during the recent 2 years. For the base of dizziness, associated past illnesses, severity, nystagmus type, and the causes of central and peripheral vertigo. Results: Female patients were 142(59.9%) and male patients were 95(40.1%). The most common age group was the 6th decade. The origin of dizziness was classified as peripheral cause(25.3%), central cause(32.9%), and others(41.8%). As to nature of the dizziness, the rotatory sense was dominant in peripheral vertigo and the floating sense and blurred vision in central vertigo. Peripheral vertigo was triggered by position change of head and body, and central vertigo by the eye movement. Audiograms I showed that most patients with central vertigo had normal hearing, but 46.7% of thoswith peripheral vertigo had an abnormal hearing disturbance. Nystagmus was more prevalent in peripheral vertigo Conclusion: History taking and physical examination played an important role in the diagnosis of dizzy patients. An important part of the diagnosis of a dizzy patient could be to evaluate the peripheral origin, the central origin and others causes. Because central vertigo may be associated with a significant neurological pathology, spectral radiological studies, including MRI and CT, are mandatory to rule out a devastating brain lesion.

남성화를 보이는 여성에서 발견된 난소의 Steroid Cell Tumor 1예

조인호,정대훈,박영미,서영진,손영실,정철회,강영미,정수전,김영남,이경복,성문수,김기태 인제대학교 2006 仁濟醫學 Vol.27 No.-

Steroid cell tumor is a rare ovarian sex cord-stromal tumor which accounts for 0.1% of all ovarian tumors. Until now, only 4 cases have been reported in domestic literatures. Steroid cell tumor often secrets testosterone and presents virilization in adult women or precocious puberty in children. Treatment is often performed by surgical removal, adjuvant chemotherapy and radiation, but completely accepted treatment was not existed. We experienced a case of steroid cell tumor, which was manifested by typical virilization in a 43-year old patient, who was previously performed hysterectomy and unilateral oophorectomy. So, we present with a brief review of the literatures.

유방암종에서 p53, cyclin D1 및 cathersin D 단백발현의 의의

문경래,김환정,이미자,기근홍,서재홍,양정원,조현진 조선대학교 2001 The Medical Journal of Chosun University Vol.26 No.2

Background and Objectives: Breast carcinoma is a heterogenous disease. The prognosis of this carcinoma correlates with various prognostic factors. The aim of this study was to assess the prognostic significance of expression of p53, cyclin Dl and cathepsin D protiens in association with various prognostic factors in invasive breast carcinoma. Materials and Methods: The relationship is presented between expression of p53, cyclin D1 and cathepsin D by immunohistochemistry and various established prognostic factors such as tumor size, histologic grade, lymph node status and extensive intraductal component in 55 cases of invasive breast carcinoma. Results: No significant correlations were observed between expression of p53, cyclin Dl and cathepsin D and tumor size. The expression of p53 correlated with high histologic grade. The expression of p53 and cyclin Dl correlated with lymph node metastasis. Conclusion: These results indicate that expression of p53 and cyclin Dl in invasive ductal carcinoma may be an important prognostic factor, closely related to the high histologic grade and lymph node metastasis.

벼 생육 및 수집에 대한 대체농자재의 영향

문영훈,최정식,엄미정,한성수 한국환경농학회 2003 한국환경농학회지 Vol.22 No.4

본 시험은 환경농업기술을 직접 농가에 파급시킬 목적으로 3년간('96~'98)수행하였던 연구결과를 토대로 1999년에 환경농업 시범마을인 남원시 대강면 방산리의 일반농가포장에서 수행한 결과이다. 계분 발효퇴비와 깻묵을 시용한 환경농업과 절충형농업 시험구의 벼 생육은 화학비료를 시용한 일반관행농업 시험구에 비하여 저조하였으며, 왕우렁이(Ampullarius insularus)를 이용한 잡초방제 효과는 제초제 2회(초·중기)살포효과보다 우수하였다. 목초액의 병해충 방제효과는 저조하였고 또한 환경농업과 절충형농업 시험구의 정조수량은 일반관행농업 시험구에 비하여 3~5% 감소 되였다. 수질은 환경농업과 절충형농업 시험구가 일반관행농업 시험구에 비하여 COD, NO₃-N, SO₄^(2-), Cl^(-)이 낮았으며, 물벼룩의 밀도는 높았다. The aim of this study was to apply the new agricultural technique developed for three years(96-98) by demonstration at an model farm of environmental agriculture located in Bangsan-ri, Dagang-myun, Namwon-city. Rice growth of environmental farming plot, which supplied with oil cake and compost fermented with chicken manure was reduced compared to that of conventional farming plot, which supplied with chemical fertilizers. Weed control by using of Ampullarius insularus in the rice paddy field was effective compared to that of control, which treated with chemical herbicide twice. The yield of rice was decreased of 3-5% in both environmental and eclectic farming good compared to that of conventional farming plot. The water quality of environmental not and eclectic farming plot were good compared to conventional farming plot. Density of water flea was high in environmental and eclectic farming plot compared to that of the conventional farming plot.

외상 후 뇌 지방색전증 1예

문정미,소정일,김용권,류진호,허탁,서정진,민용일 대한응급의학회 2001 대한응급의학회지 Vol.12 No.2

Post-traumatic fat embolism was first reported by Zenker in 1862, Von Bergmann reported the first clinical diagnosis of the fat embolism syndrome in 1873. Fat embolism has been associated with traumatic or non-traumatic disorders. Fat embolization after long bone trauma is probably common as a subclinical event. The diagnosis of fat embolism syndrome is based on the patient's history, is supported by clinical signs of pulmonary, cerebral, and cutaneous dysfunction, and is confirmed by the demonstration of arterial hypoxemia in the abscence of other disorders. Two different mechanisms cause fat to embolize: direct entry of deposit fat into the blood stream and agglutination of endogenous or exogenous plasma fat. MRI can detect a cerebral fat embolism with a higher sensitivity than cerebral CT. We report a case of post-traumatic cerebral fat embolism without pulmonary involvement, and we present a review of the literature. A 16-year girl had a traffic accident and pelvic bone fracture. Forty eight hours later severe trauma become stuporous without a focal neurological deficit. The patient received supportive therapy, and her condition improved throughout her hospital course. She was discharged with good condition after a 30-day hospital stay.

유방초음파 검사의 유용성에 대한 연구

문양인,김병학,명화련,도윤수,김계환,손정훈,김미회 한국건강관리협회 2000 건협 Report Vol.3 No.-

Helicobacter pylori가 만성위염과 위종양에 미치는 영향에 관한 면역조직화학적 밑 초미세구조적 연구

문경래,정순봉,이미숙,이미자,서재홍,김만우,박찬국 조선대학교 2001 The Medical Journal of Chosun University Vol.26 No.2

Background and Objectives : Helicobacter pylori (H. pylori) is accepted as a principal cause of chronic gastritis and an important factor in the pathogenesis of peptic ulcer and gastric malignancy. The aim of our studies is to investigate relationships between H. pylori and gastric epithelial cells. Materials ðods : One hundred and eighty one H. pylori positive cases of gastric biopsy specimens were studied immunohistochemically and ultrastructurally. Results : p53 positive staining exhibited foveolar epithelial hyperplasia in 1 out of 32 cases, regeneration in 2 out of 12 cases, incomplete intestinal metaplasia in 5 out of 41 cases, complete intestinal metaplasia in 3 out of 18 cases, chronic ulcer in 1 out of 9 cases, tubular adenoma in 2 out of 14 cases and intestinal type of adenocarcinoma in 16 out of 21 cases. Ultrastructurally, H. pylori was observed to be in direct contact with the microvilli of the target cells and was demolishing the surface microvilli. The organisms were in close contact with the uncoated cell membrance. Penetration into the gastric cells by a few organisms was associated with marked cell damage and ultimately to cell disintegration. Conclusion : Our results suggest that H. pylori plays a role in the pathogenesis of gastric cancer, leading a relatively slow, long lasting process that induces chronic active gastritis, intestinal metaplasia, dysplasia, and adenocarcinoma of intestinal type.

중학교 8학년 과학 「지구의 역사와 지각변동」 단원에서 정보통신기술(ICT) 활용 수업의 효과

정진우,문병찬,정재구,이미영 한국과학교육학회 2004 한국과학교육학회지 Vol.24 No.6

이 연구는 중학교 8학년 과학 '지구의 역사와 지각 변동' 단원에서 ICT 활용 수업 과정안을 개발ㆍ적용하여 ICT 활용 수업이 학생의 과학 탐구 능력과 과학에 관련된 정의적 특성에 미치는 효과를 알아보는 데 그 목적이 있다. 이 연구를 위해 충청남도에 소재한 D중학교 2학년 2개 학급을 선정하고 교수-학습 방법을 달리하여 약 6주간 수업하였다. 1개 학급은 실험집단으로 ICT수업 과정 안을 개발하여 ICT를 활용한 수업을 실시하였으며, 1개 학급은 통제집단으로 교과서 위주의 전통적 방법으로 수업 하였다. 학습을 하기 1주일 전에 두 집단을 대상으로 과학 탐구 능력과 과학에 관련된 정의적 특성에 대한 사전 검사를 실시하였으며, 수업이 끝난 뒤에 사후 검사를 실시하였다. 그 결과, ICT를 활용한 실험집단과 전통적 학습을 실시한 통제집단의 과학 탐구 능력 검사에서는 실험 집단에서 유의미한 차이가 나타났다. 또한, 과학에 관련된 정의적 특성에 대한 검사에서 실험집단에서 통계적으로 유의미한 차이가 나타나지는 않았지만, 3개의 과학에 관련된 특성 범위 중에서 흥미 범위에서는 통계적으로 유의미한 차이가 나타났다. 이는 ICT 활용 수업이 학생들의 과학에 관련된 정의적 특성에도 긍정적인 효과가 있음을 시사하는 것이다. The effects of learning using Information Communication Technology(ICT) on the students' scientific inquiry ability and science-related affective characteristics were analyzed in Earth History and Crust Movement units of science textbook for eighth graders. For this study, two classes of middle school second students in Chung-nam were selected and taught for six week under different teaching methods. One class is the test class taught with ICT applied teaching materials and the other class is the control class instructed by a conventional method. One week before the experiment, both classes were given tests on scientific inquiry ability and science-related affective characteristics, and they were tested again after the experiment. The results of scientific inquiry test showed statistically meaningful differences in the experimental class and the control class. The experimental class showed statistically meaningful difference in scientific inquiry test. Science-related affective characteristics test showed no statistically meaningful difference in the experimental class. Among three scientific science-related affective characteristics, especially the one fields of interests showed statistically meaningful differences which suggest that learning using ICT has a positive effect on the expanding of students' science-related affective characteristics.