Expansin으로 형질전환 된 담배의 생장 특성

신하정, 성현정, 백승준, 김보미, 엄유리, 조은정, 정찬문, 이이 충북대학교 연초연구소 2008 煙草硏究 Vol.21 No.-

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고주파수 근사 이론을 이용한 결함으로부터의 초음파 산란장 해석

정현조,김진호 한국비파괴검사학회 2000 한국비파괴검사학회지 Vol.20 No.2

본 논문에서는 체적형 결함과 균열형 결함에 대한 초음파 산란 현상을 모델링하기 위한 두 가지 이론을 설명하였다. 동탄성 Kirchhoff 근사 (EKA)와 기하학적 회절이론 (GTD)이 각각 원주형 기공과 반무한 균열에 적용되었다. 이 두 이론은 고주파수 근사법으로 알려져 있다. 모델 결함들에 평면파가 입사하는 경우의 2차원 동탄성 산란 문제를 고려하였으며 산란장을 반사계수와 회절계수의 항으로 구하였다. 원거리에서 산란파의 변위에 대한 입사파 변위의 비를 관찰 방향의 함수로 구했으며 그 결과를 경계요소법과 비교하였다. This paper describes two different theories used to model the scattering of ultrasound by a volumetric flaw and a crack-like flaw. The elastodynamic Kirchhoff approximation (EKA) and the geometrical theory of diffraction(GTD) are applied respectively to a cylindrical cavity and a semi-infinite crack. These methods are known as high frequency approximations. The 2-D elastodynamic scattering problems of a plane wave incident on these model defects are considered and the scattered fields are expressed in terms of the reflection and diffraction coefficients. The ratio of the scattered far field amplitude to the incident wave amplitude is computed as a function of the angular location and compared with the boundary element solutions.

간세포암종에 대한 간동맥 화학색전술 후 발생한 급성호흡곤란증후군

조윤원,이정미,최자윤,유동훈,차라리,오혜원,김홍준,민현주,김현진,정운태,이옥재,하창윤,이선영 이화여자대학교 의과학연구소 2013 EMJ (Ewha medical journal) Vol.36 No.1

Transcatheter arterial chemoembolization (TACE) has become an effective alternative treatment strategy for patients with inoperable hepatocellular carcinoma (HCC). Although TACE is relatively safe, acute respiratory distress syndrome associated with pulmonary lipiodol embolism is a rare and potentially fatal complication. We report a rare case of acute respiratory distress syndrome after TACE for inoperable HCC. A 75-year-old man, with huge HCC in right lobe, was treated by TACE for the first time. Seven hours after uneventful TACE procedure, he felt dyspneic and his oxygen saturation recorded by pulse oximetry (SpO2) fell to 80% despite of applying non-rebreathing mask. He underwent mechanical ventilation with a protective ventilatory strategy. We experienced a case of acute respiratory distress syndrome after TACE for HCC.

비선형 음향효과에 의한 고온 재료의 파괴인성 열화도 평가

정현조,남승훈,장경영,남영현 한국비파괴검사학회 2000 한국비파괴검사학회지 Vol.20 No.5

고온 장시간 사용재의 열화로 인한 파괴인성의 저하를 초음파 방법으로 평가하기 위하여 Cr-Mo-V 강을 실험실에서 인공 열화처리하여 열화로 인한 손상 기구를 규명하고, 열화도에 따른 초음파 파라미터의 변화를 체계적으로 관찰하였다. 초음파 파라미터로서 속도, 감쇠와 보다 최근에 개발된 비선형 음향 파라미터를 사용하였는데, 비선형 음향 파라미터가 가장 민감한 변화를 보였다. 또한 전기비저항 측정 결과와 비교할 때 두 방법 모두 전체적으로 유사한 거동을 보였다. Charpy V-notch 충격시험으로부터 구한 파면양상 천이온도(FATT)와 비선형 파라미터 사이에는 비교적 좋은 상관관계가 성립하였으며, FATT와 파괴인성(KIC) 사이의 관계식에 근거하여 열화된 발전설비 재료의 파괴인성을 초음파 비선형 파라미터의 측정을 통하여 간접적으로 구할 수 있음을 보였다. In order to develop an ultrasonic evaluation method for properties degradation of high temperature materials, a number of Cr-Mo-V steel samples were heat-treated, and their damage mechanism was examined. Ultrasonic parameters such as velocity, attenuation, and more recently developed nonlinear acoustic parameter were measured. The nonlinear acoustic parameter was found to be most sensitive to material degradation mainly attributed to the precipitation of impurities in grain boundaries. When compared to the electrical resistivity results, the nonlinear parameters showed similar behavior. There existed a relatively good correlation between the nonlinear parameter and the fracture appearance transition temperature (FATT) obtained by Charpy V-notch impact test. Based on the relationship between the FATT and the fracture toughness (KIC), correlation between the nonlinear parameter and KIC was established.

결핵성 수막염 환자에서 결핵균 30 kDa 및 32 kDa 단백항원에 대한 항체반응의 비교

백태현,김선영,이건수,김화중,김성호,조은경,박정규 충남대학교 의과대학 지역사회의학연구소 1996 충남의대잡지 Vol.23 No.2

The 30 and 32 kDa antigens from Mycobacterium tuberculosis H37Rv culture filtrates, identified as biologically important proteins in the immune responses against mycobacterial infection, were purified and used in enzyme linked ummunosorbent assay (ELISA) for the determination in specific IgG and IgM levels in cerebrospinal fluids of 15 patients with tuberculous meningitis and 17 controls with nontuberculous diseases. High reactivity to both antigens was observed in tuberculous meningitis. Mean IgG and IgM antibody levels differed significantly (P < 0.001) between patients and con trols. Mean IgG antibody levels were also higher than IgM levels in tuberculous meningitis. A comparison of the antibody levels against the 30 and 32 kDa antigen within the tuberculous meningitis patients showed higher IgG reactivity to the 30 kDa than to the 32 kDa antigen, suggesting that the antibody response of these patients is directed predominantly against the 30 kDa protein. However, the patterns of reactivity to 30/32 kDa, 30 kDa and 32 kDa antigen in individual subjects were similar. By the cut-off value adding 2 stsndard deviation to the mean absorbance of controls, the sensitivity and specificity of the IgG antibody to the 30 kDa antigen were 100% and 94.4%, respectively. These values were higher than those obtained by 30/ 32 kDa and 32 kDa antigen. From the above results, it is suggested that the 30 kDa antigen may be dominant antigen and more valuable in the diagnosis of tuberculous meningitis than 32 kDa antigen.

결핵균 30 kDa항원과 Interleukin-2 혹은 Anti-CD28의 병합이 건강인 말초혈액 림프구와 단핵구 증식에 미치는 영향

박정규,윤상호,조은경,김화중,백태현 충남대학교 의과대학 지역사회의학연구소 1994 충남의대잡지 Vol.21 No.2

Cell-mediated immunity is necessary for protection against Mycobacterium tuberculosis. T lymphocytes are thought to play a central role in cell-mediated immune response. And in vitro stimulation of leukocytes with mycobactria or their products induces synthesis and release of several cytokines, including IL-2. To study the T lymphocyte proliferative response to purified 30 kDa antigen from Mycobacterium tuberculosis H37Rv, IL-2 or their combination, peripheral blood lymphocytes and mononuclear cells isolated from healthy subjects were stimulated with the 30 kDa antigen, IL-2 or both. The proliferations of lymphocytes and molnonuclear cells to 30 kDa or 30/32 kDa antigen were significantly increased in PPD(+) group when compared to those in PPD(-) group. It is confirmed here that have shown synergy between 30 kDa antigen and IL-2(250U) in the induction of lymphocytes and mononuclear cells proliferation in PPD(+) group. The proliferative response reflects synergy between two separate activation stimuli. And macrophage-like cells existed in peripheral blood mononuclear cells were shown to help the synergy of the proliferative response to mycobacterial antigen and IL-2. The proliferative responses of lymphocytes and mononuclear cells to 30 kDa antigen were more increased than those to 30/32 kDa antigen. So it was suggested that 30 kDa antigen was the more immunogenic antigen than 30/32 kDa antigen.

HL-60 세포주를 이용한 결핵균항원의 세포성면역반응의 분석

박정규,강윤중,김운옥,임재현,송창화,조은경,김화중 충남대학교 의학연구소 2001 충남의대잡지 Vol.28 No.2

Most persons who become infected with M. tuberculosis mount a protective immune response and remain clinically well, the only evidence of infection being development of a positive tuberculin skin test. Five to 10% develop tuberculosis disease within the first 2 years after infection (primary tuberculosis) or thereafter (reactivation tuberculosis). Acquired resistance against tuberculosis paradigmatically rests on cell-mediated immunity, with the major factors being mononuclear phagocytes and T Lymphocytes. While the former cells act as the principal effectors, the latter ones serve as the predominant inducers of protection. The usefulness of the single dose of BCG routinely given in childhood in many developing countries in preventing far more commonly occurring tuberculosis in adults is in doubt. An effective and safe vaccine against tuberculosis is sorely needed. A subunit vaccine are capable of inducing protective immunity and could have substantial advantages over BCG or other whole-bacterium vaccines. The human promyelocytic cell line HL-60 adopted characteristic macrophage-like properties, including adherence and CD14 expression after a period of continuous culture at high ambient CO_(2) concentration. When HL-60 cells were cultured with 1,25-dihydroxyvitamin D_(3) for 4 days, the cells acquired the activity to potentiate T cell proliferation by the 30 kDa or 38 kDa antigen of Mycobacterium tuberculosis H37Rv Therefore, vitamin D-treated HL-60 cells showed the function of the antigen presenting cells.

MTT비색법에 의한 결핵균 30-kDa항원이 임파구 증식에 미치는 영향

백태현,김민경,박정규,김화중,조은경,최대경 충남대학교 의과대학 지역사회의학연구소 1991 충남의대잡지 Vol.18 No.1

The cell mediated immune response appears to result from the specific recognition of an antigen. by T lymphocytes. It has been well recognized that T cell play an important roles in the induction of tuberculin hypersensitivity and immunity to tuberculosis. In order to evaluate effect on T lymphocyte proliferative response to purified 30-kDa antigen from Mycobacterium tuberculosis, stimulation effects of peripheral blood lymphocytes with 30-kDa antigen, crude antigen and PHA were measured by MTT colorimetric assay. Three groups of healthy subjects, representing PPD(+), PPD() and PPD(-) persons, were investigated. The proliferative response to 30-kDa antigen were elicited the plateau at concentration of 20 to 0.1㎍/ml, crude antigen showed rapid reduction as decreasing the concentration of antigen, for the PPD(+) person. Both antigens, at concentration of <1㎍/ml, failed to stimulate lymphocytes of the PPD(-) person. Therefore the concentration of 30-kDa antigen to induce optimal stimulation was 1㎍/ml. The lymphocyte proliferation to 30-kDa antigen and crude antigen were significantly increased in PPD(+) group when compared to those in PPD(-) group, but PHA response was no significant difference. These results suggest that 30-kDa antigen could stimulate lymphocyte from PPD(+) population and MTT colorimetric assay could be applied to assess proliferative response of lymphocyte.

결핵균 30-kDa와 32-kDa 단백항원의 분리정제와 마우스 비장세포 증식에 미치는 영향

백태현,오명주,김화중,조은경,박정규,최대경 충남대학교 의과대학 지역사회의학연구소 1993 충남의대잡지 Vol.20 No.1

Identification and characterization of individual components of M.tuberculosis have long been a focus of research on tuberculosis. The 30-kDa and 32-kDa antigens are major constituents of M bovis BCG and M. tuberculosis culture fluids. Because 30-kDa and 32-kDa antigens are partially identical, these antigens are difficult to purify in large amounts by biochemical techniques. This study was performed to purify the 30-kDa and 32-kDa antigens complexes primarily purified by 50% ammonium sulfate precipitation, hydroxylapatite chromatography and Sephadex G-75 gel filtration. And then further purification for separation of the two antigens was accomplished on preparative isoelectric focusing. Recovery of 30-kDa and 32-kDa antigens during above the purification procedures were 28% and 14%, respectively, and 147.0 and 59.8-fold purification were showed, respectively. On silver stained SDS-PAGE gels, the purified 32-kDa antigen gave a single band at 32-kDa molecule, while 30-kDa antigen gave one major band at 30-kDa molecule and faint additional band at 32-kDa. The pI of 30kDa-and 32-kDa antigens were 4.3 and 4.6, respectively. The partial identity between these two antigens was observed through the same pattern of reactivity of antigens in the ELISA and precipitation pattern of immunodiffusion. We also examined the immunological activities of both purified antigens by splenocyte proliferation of BCG-infected mice. Proliferative response to the 30-kDa and 32-kDa antigens did not show significant difference. However, macrophage-like cells existed in spleen cells from BCG-infected mice were involved in the suppression of the proliferative response to mycobacterial antigen. These results suggest that the 30-kDa and 32-kDa antigens could be effectively purified by the IEF and reactions of partial identity between the two antigens were found. However, 30-kDa antigen was the more immunogenic antigen than 32-kilodalton.

전기로 더스트의 환경안정적 재활용을 위한 CI 제거에 관한 실험 고찰

박병현,조영민,유정근 경희대학교 환경연구소 2001 環境硏究 論文集 Vol.10 No.-

Steel slag is a kind of industrial waste generating from the steel refining process in an Electric Arc Furnace. The annual production of this waste is more than 200.000 tonnes, and has recently been considered handful. Thus, in order to enhance the reuse of the steel slag, the present work attempted to reduce the chlorine element in the slag by using chemical treatment. Chlorine elements were cleansed by addition of a few solvents such as acetone, methanol and ethanol through dilution with water. Mohr titration was applied to measure the chlorine concentration in terms of reaction time, dilution ratio and temperature. As a result, methyl alcohol was found the most effective at high reaction temperature with a lengthened reaction.