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α1,3-Gal Knock out Pigs Increases N-Glycolylneuraminic Acids
Jong-Yi Park,Mi-Ryung Park,Hong-Thuy Bui,Deug-Nam Kwon,Min-Hui Kang,Mihye Oh,Jae-Woong Han,Ssang-Goo Cho1,Chankyu Park,Hosup Shim,Hye-Min Kim,Man-Jong Kang,Jin-Ki Park,Jeong-Woong Lee,Kyung-Kwang Lee 한국동물번식학회 2012 Reproductive & Developmental Biology(Supplement) Vol.36 No.2s
In this study, we examined whether Hanganutziu-Deicher (H-D) antigens are important as an immunogenic non-a1,3-galactose (Gal) epitope in pigs with a disrupted a1,3- galactosyltransferase gene. The targeting efficiency of the AO blood genotype was achieved (2.2%) in pig fibroblast cells. A total of 1800 somatic cell nuclear transfer (SCNT) embryos were transferred to 10 recipients. One recipient developed to term and naturally delivered two piglets. The a1,3-galactosyltransferase activity in lung, liver, spleen, and testis of heterozygote a1,3-galactosyltransferase gene knockout (GalT-KO) pigs was significantly decreased, whereas brain and heart showed very low decreasing levels of a1,3- galactosyltransferase activity when compared to those of control. Enzyme-linked lectinosorbent assay showed that the heterozygote GalT-KO pig had more sialyla2,6- and sialyla2,3- linked glycan than the control. Furthermore, the heart, liver, and kidney of the heterozygote GalT-KO pig had a higher N-glycolylneuraminic acid (Neu5Gc) content than the control, whereas the lung of the heterozygote GalT-KO pig had Neu5Gc content similar to the control. Collectively, the data strongly indicated that Neu5Gc is a more critical xenoantigen to overcoming the next acute immune rejection in pig to human xenotransplantation.
α1,3‐Gal Knock out Pigs Increases N‐Glycolylneuraminic Acids
Jong‐Yi Park,Mi‐Ryung Park,Hong‐Thuy Bui,Deug‐Nam Kwon,Min‐Hui Kang,Mihye Oh,Jae‐Woong Han,Ssang‐Goo Cho,Chankyu Park,Hosup Shim,Hye‐Min Kim,Man‐Jong Kang,Jin‐Ki Park,Jeong‐Woong Lee,Kyung‐Kwang Lee,J 한국동물번식학회 2012 Reproductive & developmental biology Vol.36 No.2
( Seok Won Jung ),( Neung Hwa Park ),( Jung Woo Shin ),( Bo Ryung Park ),( Chang Jae Kim ),( Jong Eun Lee ),( Eun Soon Shim ),( Jeong A Kim ),( Young Hwa Chung ) 대한내과학회 2014 대한내과학회 추계학술대회 Vol.2014 No.1
Background: Based on the current knowledge of cancer molecular pathogenesis, polymorphic variations are considered to be important for hepatocellular carcinoma (HCC) development. Serial Sanger sequencing of suspected HCC genes, while the current molecular diagnostic method of choice, can be slow and expensive. A comprehensive panel test for simultaneous screening of mutations in all known HCC-associated genes would be a more effective diagnostic strategy. Methods: Using a highly multiplexed PCR-based target enrichment method in conjunction with next-generation sequencing (NGS), we performed mutation detection in 10 hepatitis B virus (HBV)-associated HCC cases and validated in 285 HCC patients by Sanger sequencing. Results: Variants (rs3812603 and rs9411206) of NOTCH1 gene were signifi cantly associated with the postoperative recurrence of 10 HBV-associated HCC patients by NGS, which were validated in 285 independent HBV-associated HCC cases. Three variants were located in close. In addition, NOTCH1 rs9411206 was signifi cantly associated with distant metastasis of HCC. Conclusions: The novel NOTCH1 gene variants are associated with a signifi cantly decreased risk of postoperative recurrence of HBV-associated HCC. Although the variant accounts for a small fraction of all HCCs, this fi nding has implications for postoperative recurrence risk assessment and may provide new mechanistic insights into HCC.
단클론성 유무가 저등급 위점막연관림프조직형 림프종의 임상양상 및 제균요법 후 초기 조직학적 관해에 미치는 영향
김진호,홍원선,민영일,정훈용,명승재,강경훈,김기락,허주령,최승목,심용희,이숭한,양석군 대한소화기학회 2001 대한소화기학회지 Vol.37 No.4
Background/Aims: There has been little description about the clinical significance of B-cell monoclonality in primary low-grade gastric mucosa-associated lymphoid tissue (MALT) lymphoma. We investigated the effects of B-cell monoclonality on early response to Helicobacter pylori (H. pylori) eradication therapy in patients with H. pylori-positive low-grade gastric MALT lymphoma. Methods: Twenty-nine patients with H. pylori-positive low-grade gastric MALT lymphoma were consecutively enrolled. We performed diagnostic tests including esophagogastroduodenoscopy (EGD), endoscopic ultrasonography and CT scan for the patients. PCR with PAGE was used to analyse the patterns of immunoglobulin heavy chain gene rearrangement. All 29 patients received H. pylori-eradication therapy. They were then followed up at regular intervals by EGD with biopsy. Results: Fifty-five percent (16/29) showed a single band in PAGE after PCR, suggesting a monoclonal proliferation of B-cell lineage. Regardless of B-cell monoclonality, there was no difference in histologic regression rates after 3 months of eradication therapy. However, the MALT lymphomas with B-cell monoclonality showed more ulcerous lesions (p$lt;0.01) and deeper infiltration of tumor into gastric wall (p$lt;0.05) than those without B-cell monoclonality. Conclusions: These results suggest that PCR-based B-cell monoclonality may not affect early therapeutic response to H. pylori-eradication therapy in gastric MALT lymphoma in spite of the more aggressive histologic morphology.