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PCR을 이용한 국내시장에 유통중인 유전자재조합 콩 및 가공식품의 모니터링
김묘영,김재환,김현중,박선희,우건조,김해영 한국응용생명화학회 2003 Applied Biological Chemistry (Appl Biol Chem) Vol.46 No.4
본 연구에서는 PCR을 이용하여 국내시장에 유통중인 원료콩과 가공식품에 epsps 또는 pat 유전자가 삽입된 유전자재조합 콩(GMS)의 사용여부를 모니터링하였다. 이러한 GMS의 검출을 위해 3쌍의 primer set을 제작하였고, 각각의 primer들은 GMS에 삽입된 유전자와 특이적으로 반응하여 PCR산물을 생성하였다. 2001년 표시제가 시행되기 이전에 생산된 총 가공식품과 이후의 제품에 대해 각각 모니터링을 수행하였으며, 표시제 이전에 생산된 제품의 경우 대부분의 미국산 원료에서 esps가 삽입된 GMS가 검출되었으나, 표시제 이후에는 검출되지 않았다. A method using PCR was developed for the monitoring of genetically modified soybean (GMS) and GMS derived foods utilized in the market. We designed 3 pairs of specific oligonucleotide primers based on epsps and pat inserted in GMS and ferritin gene as internal standards. Template DNAs isolated from soybean and processed foods were used for multiplex PCR with 3 primer sets. PCR, used with specific primer sets for GMS detection, showed the amplified DNA fragments with GMS template DNA. In this study, GMS containing epsps was detected from soy processed foods manufactured before GM food labeling system, however, GMS containing epsps or pat was not detected from soy processed foods manufactured after GM food labeling system.
신경회로망에 의한 용접 결함 종류의 정량적인 자동인식 시스템 개발에 관한 연구
김재열,김영석,김병현,유신,김훈조,정진홍 한국공작기계학회 1997 한국생산제조학회지 Vol.6 No.1
A neural network approach has been developed to determine the depth of a surface breaking crack in a steel plate from ultrasonic backscattering data. The network is trained by the use of feedforward three-layered network together with a back-scattering algorithm for error correction. The signal used for crack insonification is a mode converted 70˚ transverse wave. A numerical analysis of back scattered field is carried out based on elastic wave theory, by the use of the boundary element method. The numerical data are calibrated by comparison with experimental data. The numerical analysis provides synthetic data for the training of the network. The training data have been calculated for cracks with specified increments of the crack depth. The performance of the network has been tested on other synthetic data and experimental data which are different form the training data.
Choi, Dukhyun,Choi, Min-Yeol,Choi, Won Mook,Shin, Hyeon-Jin,Park, Hyun-Kyu,Seo, Ju-Seok,Park, Jongbong,Yoon, Seon-Mi,Chae, Seung Jin,Lee, Young Hee,Kim, Sang-Woo,Choi, Jae-Young,Lee, Sang Yoon,Kim, Jo WILEY-VCH Verlag 2010 ADVANCED MATERIALS Vol.22 No.19
<B>Graphic Abstract</B> <P>The cover shows an image of fully rollable transparent nanogenerators synthesized using chemical vapor deposition grown large-scale graphene sheets as transparent electrodes and piezoelectric ZnO nanorod arrays. Sang-Woo Kim, Jae-Young Choi, and co-workers report on p. 2187 the electrical and structural stability of the nanogenerators, with excellent charge scavenging performance under external mechanical loads such as bending and rolling. This study shows that graphene-based nanogenerators are very promising for self-powered rollable transparent device applications. <img src='wiley_img_2010/09359648-2010-22-19-ADMA201090066-content.gif' alt='wiley_img_2010/09359648-2010-22-19-ADMA201090066-content'> </P>
김원,최유성,이태양,최재곤,홍완해,조병욱 조선대학교 생산기술연구소 2003 生産技術硏究 Vol.25 No.1
This study explores the potential of improving the physical properties of poly(trimethylene terephthalate) (PTT) by inducing thermotropic liquid crystalline polymers. The TLCP was synthesized by polycondensation reaction of terephthaloyl chloride with corresponding diols. The TLCP used was copolymers containing random PTT groups on the polymer backbone. The PTT moieties were used to increse compatibility between the PTT and the TLCP. The TLCP showed the nematic phase above those melting temperature(T_m) The TLCP seems to act as plasticizers and nucleating agents in the matrix polymers Liquid crystalline phase did not reaveal any significant macrophase separation and thermal degradaton under the temperature of processing condition.
고사리(Pteridium aquilinum)뿌리 전분의 이화학적 특성에 관한 연구 : 제2보 : 전분의 물리적 특성 Ⅱ. Physical Properties
曺哉銑,金成坤,李啓瑚,權泰完 경희대학교 부설 식량자원개발연구소 1981 硏究論文集 Vol.2 No.-
고사리뿌리 전분의 이화학적인 특성연구의 일환으로 실시된 물리적 특성시험 결과는 다음과 같다. 전분입자의 미구조의 결합력을 보기 위한 흡수도, 팽운력, 용해도 시험결과 감자전분이나 타피오카전분보다 강한 결합을 하고 있으며 곡류전분보다는 약하며 팽윤력이 single-stage pattern을 이루고 있었다. 가열에 의한 전분입자의 복굴절성 상실, α-아밀라아제에 의한 소화성 및 X-선 회절둥으로 전분의 호화과정을 살펴본 결과 55-60℃에서 호화가 시작되고 60~70℃에서는 95%이상 호화되었다. 또한 3~6%전분용액의 가열에 따른 점도변화를 amyl-ograph를 사용하여 실험한 결과 pasting temperature 62~68℃, 최고점도 80~840BU, 50℃로 냉각후의 점도는 110~555BU였다. 따라서 다른 전분에 비해서 점도가 낮으나 setback이 현저히 느렸다. 수분함량 50%의 전분겔의 경도는 팥 전분보다는 약하고 감자나 밀전분에 비해서는 현저히 높았다. 전분겔의 저장중 노화속도는 밀 전분보다는 느리고 파지오카 전분보다는 빨랐다. 고사리뿌리 전분은 팥 전분겔특성에 큰 변화를 주지않지만 노화를 억제하였고 밀 전분겔에 첨가시에는 겔의 경도가 저하되어 부드러워지는 경향을 보였다. The gelatinization phenomena of bracken root starch were examined by means of the loss of birefringence, degree of digestibility by amylase and X-ray diffraction. These results indicated that gelatinization temperature of the starch was 55∼60℃ and over 95% of starch were gelatinized at the temperature between 60 and 70℃. The swelling power of the bracken root starch was much less steeper than that of potato or tapioca starch. Amylograph data on the various starch concentrations showed the pasting temperature of 62∼68℃, peak height of 80∼840 Brabender unit (BU) and peak after cooling to 50℃ of 110∼555 BU. According to the information obtained from amylograph data, the bracken root starch showed low set back. The rate of retrogradation of the starch as tested by Texturometer was slower and faster than potato and tapioca starches, respectively.
50,000rpm급 초고속 주축계의 정적/동적/열적 특성 해석
김석일,조재완,이원재,이용희 한국공작기계학회 2003 한국공작기계학회 춘계학술대회논문집 Vol.2003 No.-
This paper concerns the static, dynamic and thermal characteristics analysis of a high-speed spindle system for horizontal machining centers with φ45mm × 50,000rpm. The spindle system is designed based on the angular contact ceramic ball bearings, built-in motor, oil-air lubrication method and oil jacket cooling method. The structural and thermal analysis models of spindle system are constructed by the finite element method. The static and dynamic characteristics are estimated based on the static deformation, modal parameter, mode shape and frequency response function, and the thermal characteristics are estimated based on the temperature rise, temperature distribution and thermal deformation. The analysis results illustrate that the designed spindle system has excellent structural and thermal stabilities.
Organoclay/TLCP nanocomposites의 제조 및 특성
김원,최재곤,조병욱 조선대학교 생산기술연구소 2003 生産技術硏究 Vol.25 No.2
The organoclay/TLCP(Thermotropic Liquid Crystal Polymer) nanocomposites were prepared by in-situ polymerization. To discuss the effect of the organoclay contents on the polymer properties, we prepared organoclay/TLCP nanocomposites of 2, 5, 10wt% in organoclay contents. The organoclay/TLCP nanocomposites were characterized by scanning electron microscopy(SEM), X-ray diffractometer(XRD), dynamic mechanical thermal analysis (DMTA), differential scanning calorimetry (DSC), and thermogravimetric analyzer(TGA). The bending moduli of organoclay/TLCP nanocomposites were improved with increasing organoclay contents. The TLCPs and organoclay/TLCP nanocomposites showed the nematic phase above those melting ternperature(T_(m)). The TLCPs and organo clay/TLCP nanocomposite seem to act as plasticizers and nucleating agents in the matrix polymers.
진공청소기 팬 모터의 진동 및 소음원인 분석을 위한 유동해석
김재열,곽이구,안재신,양동조,송경석,박기형 한국공작기계학회 2004 한국생산제조학회지 Vol.13 No.4
Recently technology resulted in highly efficient and multiple-functional electric appliances considering environmental problems. One of the environmental problems is noise of a product in respect to its function. A vacuum cleaner is an essential electric appliance in our daily lives. However, severe noise resulted from high motor speed for improving the function of the appliance is a nuisance for the user. This noise is caused by vibration from various parts of the appliance and fluid noise during a series of intake and exhaust processes while rotating the impeller connected to the axle at a high speed of the fan motor inside the vacuum cleaner rotating around 30,000-35,000rpm. Despite the fact that many researchers conducted studies on reducing the noise level of the fan motor in a vacuum cleaner, only few studies have been conducted considering both the theoretical and experimental aspects using fluid analysis by measuring vibration and noise. Moreover, there has not been a study that accurately compared major noise data obtained considering both of the aspects. In this study, both aspects were considered by considering the following experimental and theoretical methods to verify the major causes of noise from the fan motor in a vacuum cleaner.