Novel Methylation Biomarker for Non-invasive Diagnostics in Lung Cancer

오태정,( Chang Hun Lee2 ),( Min Ki Lee ),( Yeul Hong Kim ),( Sang Yull Lee ),( Hyo Sung Jeon ),( Shin Yup Lee ),( Seung Soo Yoo ),( Jae Yong Park ),( Sung Whan An ) 대한결핵 및 호흡기학회 2012 대한결핵 및 호흡기학회 추계학술대회 초록집 Vol.114 No.-

To identify aberrantly hypermethylated DNA in lung cancer cells we established a genome-wide analysis for hypermethylation sites, namely Methyl DNA Isolation and Amplification (MeDIA) coupled-CpG microarray analysis. In the comprehensive methyaltion profiling analysis between human lung cancer, A549 cells and normal NHBE cells, we observed that several clusters of genes show a significant level of aberrancy in CpG island methylation pattern in cancer cells compared to normal cells. We further identified PCDHGA12 gene as a new marker of non-invasive diagnostics for lung cancer based on followings. 1) Transcription of PCDHGA12 gene is reactivated after treatment of A549 cells with demethylating agent. 2) Bisulfide clonal-sequencing reveals that CpG island of PCDHGA12 shows a distinctive differential methylation between two cell lines. 3) Pyrosequencing-based quantitative methylation assay for such region in tumor and non-tumorous tissues from lung cancer patients shows aberrant hypermethylation in 37 (92%) of the 40 tumor tissues. In clinical validation by pyrosequencingin induced-sputum of lung cancer patients (n=87) and healthy controls (n=51), we observed aberrant hypermethylation incident at significantly elevated level in samples derived from lung cancer patients. According to the optimal threshold calculated by ROC curve analysis, sensitivity and specificity of PCDHGA12 was 86.2% and 82.4%, respectively. PCDHGA12 methylation status could be a potential methylation biomarker alone or combined with others for the screen and the detection of relapse of lung cancer.

矢狀分割骨切斷術에 依한 顎矯正術의 統計學的 硏究

辛曉根,陳宇政,李俊禮,金晤煥,李炫尙 大韓顎顔面成形再建外科學會 1997 Maxillofacial Plastic Reconstructive Surgery Vol.19 No.2

Recently, combined with social complexity, increment of medical demand and supply and the change of esthetic category, admission and operation of the patients of facial deformity have been changed with annual change. This study was conducted in the concept of helping the overall character of orthognathic surgery in future understood and being an important index in the establishment of better treatment course, through the patient, on whom was operated orthognathic surgery via sagittal split ramal osteotomy in our Dept. of Oral & Maxillofacial Surgery, Chonbuk National University Hospital from 1991. 1. 1 to 1995. 12. 31. The results were obtained as follows. 1. The ratio of male to female was 1 : 1.33 and the ages between 16 and 25 year was 73.6%, and the mean age was 20.4 years. 2. The ratio of setback amount between 6 to 15mm was 84.6% and the advancement amount between 1 to 10mm was 89% and the mean amount of movement was 9.0mm in setback, and 3.6mm in advancement. 3. After removal of maxillomandibular fixation(MMF), the distinction of sex was not statistically significant in ROM. 4. The ROM following methods of fixation was statistically significant in 3rd(P<0.05), 8th, and 12th week(each P<0.01), with faster rehabilitation in rigid fixation which had shorter MMF period. 5. The rehabilitation of ROM following the operation methods was statistically significant in 1st(P<0.05), 2nd, 3rd, 4th, 6th, 8th, and 12th week(each P<0.01) with faster rehabilitation in the case of SSRO than Lefort I / SSRO (Two jaw surgery). 6. The rehabilitation of ROM following directions of mandibular movement did not manifest a statistically significant difference in both setback and advancement.

고등학생의 성폭력 경험과 인식

신효식,이경주 한국 가정과 교육 학회 2003 한국가정과교육학회지 Vol.15 No.1

The purpose of this study is to investigate the cognition and experience of sexual violence and to present the influential factors of the cognition of sexual violence. The subjects were 835 high school students living in Kwangju and Chonnam. The data analysis used Mean, Standard Deviation, T-test, F-test, and Multiple Regression Analysis. The major findings is as follows; 1. There are meaningful differences in the experience of sexual violence between male and female adolescents. 2. There are meaningful differences in the cognition of sexual violence in sex, sex-role attitude, experience of obscene materials and sex education. 3. The variables which affect cognition of sexual violence is sex-role attitude, sex and experience of obscene materials that were explained about 10%.

Lidocaine의 산성도가 경막외 마취에 미치는 영향

이원형,신효철,신용섭 충남대학교 의과대학 지역사회의학연구소 1990 충남의대잡지 Vol.17 No.1

The affective factors on regional anesthesia are lipophilic-hydrophilic balance of local anesthetics, protein binding, hydrogen concentration, structure of intermediate chain, and etc. Local anesthetics in solution exit in a chemical equilibrium between the basic uncharged form(B) and the charged cationic form(BH^+) by Henderson-Hasselbalch equation(PH_a=PK+log[B]/(BH^+)). The increasement of uncharged form brig about rapid onset, good quality, and long duration of anesthesia. Eight patients in group Ⅰ were given 2% lidocaine 21.7ml with 1: 200,000 epinephrine(pH 5.1), and eight patients in group Ⅱ were given 2% lidocaine 20ml with 1: 200,000 epinephrine added NaHCO_3 1.7ml(pH 7.2) to determine the epidural analgesic effect of the pH-adjusted solution. The results are summerized as follow 1. The onsets of analgesia and surgical anesthesia were significantly rapid in group Ⅱ. 2. The levels of analgesia were raised more rapidly in group.

Quick Response 기반의 Mass-Customization구현을 위한 점포유형에 따른 직물감성에 관한 소비자 태도 연구

신상무,이효정 한국의류학회 2002 한국의류학회지 Vol.26 No.11

Quick Response based Mass-Customization can be produced and distributed customized goods and services on mass basis in apparel e-business. Because consumers can't touch and feel the apparel products in e-business, they tend to have the negative buying behavior. The purpose of this study is to investigate the differences of consumer's texture sensibility of apparel products based on marketing channels(on-line/off-line). Two types of questionnaires for on-line and off-line were used to assess consumer sensibility on apparel fabric. The 8 swatches were selected in regard to the previous literatures. 205 questionnaires for each type (on-line/off-line) were distributed. Statistical devices were t-test, mean and standard deviation with SPSS_(10.0.) The result of this study was showed that there were partially significant differences on consumers' texture sensibility on apparel products between on-line and off-line. Under on-line environment, consumers perceived corduroy as warm, strong, and sandy. taffeta as warm, sandy, and glossy, denim as sandy, and warm, organza as sandy, and thin, satin as sandy, dense, and modern, chiffon as sandy, and fiat, velvet as warm, and soft, single jersey as warm, soft, and comfortable. Therefore, apparel firms cooperating QR based Mass-Customization in e-business have to pay attention to the differences on consumers' texture sensibility of on-line apparel products from those of off-line.

Sol-Gel Process를 利用한 Y-SrTiO_3 微分體 合成

申孝順,이병교 경북대학교 산업기술연구소 1992 産業技術硏究誌 Vol.20 No.-

Y-SrTiO_3 powders are synthesized by pechini process as a kind of sol-gel process and compared with powders by solid state reaction with respect to powder properties. As a result, calcination temperature is lowered about at 500℃. Furthermore, obtained powders are fine and homogeneous. And these powders are agglomerated and the size of primary particles is about 30㎚.

한국전통생활문화에 관한 고찰

이숙,홍윤호,신윤숙,신효식,한경미,최정미,김은정 全南大學校家政科學硏究所 2002 生活科學硏究 Vol.12 No.-

This study is objected on preliminary research for more scientific and wider studies on the Traditional Living Culture of Korea. For this purpose, studies about clothing, food, home management, Traditional Living Culture were reviewed and analysed. The results were as follows : First, Clothing culture studies concentrated on ritual clothings, Second, Food Culture studies included history of traditional food development and recipes. Third, in the field of home management value system, attitudes and parent-child relations were studied,

Ascorbate Peroxidase 유전자의 도입에 의한 식물의 형질전환

이인애,이효신,배은경,김기용,이병헌,손대영,조진기 Plant molecular biology and biotechnology research 2002 Plant molecular biology and biotechnology research Vol.2002 No.-

This study was conducted to obtain the transformed tobacco (Nicotiana tubacum) plants with cytosolic ascorbate peroxidase gene(ApxSC7) using Agrobacterium tumefaciens LBA4404. A cDNA encoding the cytosolic ascorbate peroxidase of strawberry, ApxSC7, was introduced into tobacco plants via Agrobacterium-mediated gene transfer system. The expression vector, pIG-AP8, harboring ApxSC7 gene was used for production of transgenic tobacco plants. A large number of transgenic plants were regenerated on a medium containing hygromycin. Integration of ApxSC7 gene was confirmed by PCR and Southern blot analyses with genomic DNA. Northern blot analyses revealed that the pIGap8 gene was constitutively expressed.

枸杞 Lycium chinense Miller의 果實이 實驗的 糖尿에 미치는 影響

李珩九,姜孝信,鄭址昌 中央醫學社 1975 中央醫學 Vol.28 No.6

The influence of fruit of Lycium chinense Miller on the blood sugar levels and serum transaminase (SGP-T, SGO-T) activities from alloxan-diabetic rabbits were observed. The Lycii Fructus were extracted with methanol, and the extracted sample was orally administrated to normal and alloxan diabetic rabbits. As for the blood sugar levels and serum transaminase activities of normal rabbits no significant variation were detected by administration of the extracted sample. The blood sugar levels and serum transaminase activity of normal rabbits were significantly increased by administration of the alloxan. The increased blood sugar levels of 'the alloxan-diabetic rabbits were gradually decreased by administration of the extracted samples, and were returned to the control levels after 12 day. The increased transaminase activities in serum of the alloxan-diabetic rabbits were significantly decreased after 96 hours. According to the above results, it would be concluded that the extract of Lycii Fructus influences for the recovery of the increased amount of blood sugar levels and serum transaminase activities by alloxan.

노부모의 성인자녀와의 갈등 및 우울에 관한 연구

이윤정,이신숙,신효식 全南大學校家政科學硏究所 1994 生活科學硏究 Vol.4 No.-

This study was to investigate the depression of the aged parents perceived through the conflicts between the aged parents and their adult children. The subjects of the study were 469 old parents, consisted of 234 male and 235 female over 60 years old lives in Kwangju. The datas were analysed by frequency, percentage, mean, standard deviation, oneway ANOVA, t test, Duncan's test and multiple regression using SAS program and the results are as follows. 1) In the conflict between the aged parents and their adult children which the aged parents perceive, the mean values are 32.54 in the case of old father and 34.26 in old mother from the average point. The average values of the depression the aged parents perceive are 26.03 and 28.35 each in old father and old mother and show the level of depression over the middle value. 2) The background variables which show the significant differences of the conflict between the aged parents and their adult children are marital status, age, education, pocket money, health and activity of the aged parents. 3) The background variables which show the significant difference in the depression the aged parents perceive are marital atatus, education, pocket money, health, activity, support type and occupation of the aged parents. 4) The depression and conflict between the aged parents and their adult children show high correlations and the greatest variable as the effect of conflict on the depression is emotional conflict. In addition, significant difference is shown depending on health, value and economic conflict and its explanatory power is 50%.