인체 대장암 및 자궁경부암에서 PCR-SSCP법을 이용한 Ki-ras 암유전자의 점돌연변이에 관한 연구

박영홍,백낙환,김현찬,김상효,홍관희,김기태,이기영 인제대학교 1994 仁濟醫學 Vol.15 No.2

인체 대장암 20례와 자궁경부암 10례의 암조직을 대상으로 c-Ki-ras 유전자 codon 12와 13에서의 점돌연변이를 알아보고자 polymerase chain reaction(PCR)과 single-strand conformation polymorphism(SSCP) 검사법을 시행하였다. 대장암의 경우 20례중 9례(45%)에서, 자궁경부암의 경우 10례중 1례(10%)에서 양성으로 나왔다. 대장암에서는 codon 12에서 GGT가 TGT로의 치환이 4례로서 가장 많았으며, AGT, CGT로의 치환이 각 1례였고 codon 13에서는 GGC가 GAC로의 치환이 2례, TGC로의 치환이 1례인 것으로 나타났다. 자궁경부암의 경우 codon 12에서 GGT가 AGT로의 치환이 1례인 것으로 나타났다. In an attempt to clarify the role of genetic alteration in the genesis of human colorectal and cervical cancers, tissue specimens from 20 patients with colorectal cancer and 10 patients with cervical cancer were examined for the presence of point mutation in K-ras2 exon 1 by single strand conformation polymorphism analysis of PCR product. Exon I of c-Ki-ras2 was amplified by polymerase chain reaction(PCR) and comparison was made between the normal and mutated genes by nondenaturing polyacrylamide gel electrophoresis(PAGE) of PCR product and nucleotide sequence analysis using asymmetric PCR with direct sequencing. Genomic DNA from white blood cells were used as normal control and those from A427 cell line were used as mutated control. 1.PCR product from A427 cell line showed a distinct migration shift pattern compared to the normal control in PAGE and the direct sequencing indicated that nucleotide sequence of codon 12 was mutated from GGT to GAT. In addition, there was a loss of normal allele in A427 cell line. 2.PCR product from 9 cases(45%) out of a 20 colon cancer patients showed migration shifts in PAGE, and all of these 9 patients invariably demonstrated mutation of ras gene, either in codon 12(6 cases:30%) or 13(3 cases:15%). The base substitutions in codon 12 were: from GGT to AGT(1), CGT(1), or TGT(4). Base changes in codon 13 were from GGC to TGC(1) or GAC(2). 3.PCR product from 1 cases(10%) out of a 10 cervical cancer patients showed migration shrifts in PAGE, Invariably demonstrated mutation of ras gene in codon 12. The base substitutions was from GGT to AGT(1) TQE incidence of point mutation of c-Ki-ras in colon cancer was high, however, it was low in cervical cancer, which showed the relation of ruts gene mutation with colon cancer. PCR -SSCP analysis is a simple, rapid and efficient method of detection of point mutation, especially when dealing with multiple samples.

대장암 주변에서 관찰되는 백색반점의 병리조직학적 특성과 임상적 의의

김효종,조진만,장린,동석호,김윤화,장영운,박일랑,김병호,이정일 대한소화기내시경학회 1999 Clinical Endoscopy Vol.19 No.4

Background/Aims: To investigate the difference between colorectal adenocarcinomas with white spots (foamy cells) and those without white spots, clinically and histopathologically, were examined 37 cases of colorectal adenocarcinomas were classified in this study. Methods: Two groups: those with white spots and those without. In each case, evidence of lymph node and liver metastasis was sought. Immunoreactive staining for macrophage was performed. Results: Increased incidence of exophytic tumors was found (73%, p=0.028) in the group with white spots. The incidences of lymph node metastasis were 33% vs. 45% between the group with white spots and the group of without, but the difference was not statistically significant. Conclusions: These results suggested that foamy cells might have a beneficial role in colorectal cancer, although they were not statistically significant. Therefore, further prospective study be warranted.

구조화된 환자교육에 대한 연구논문 분석 : 고혈압 환자를 중심으로 forcused on the patients with hypertension

박청자,이경희,고효정,권영숙,김정남,박영숙 대한보건협회 2003 대한보건연구 Vol.29 No.2

This study was conducted to analyze the nursing research methodology and the key concepts used in articles related hypertension published in the RICH. The purpose of this study was for reflecting the trends of the research on the structured patient education focused the hypertensives. The results were as following. 1. There were 119 research studies related hypertension in RICH from 1994 to 2002. The number increased in 1997. Research studies of 98 have done since 1997 and it is 82% of the total research studies. 2. The most of the research studies are non-degree research studies. Research studies with fund have been rapidly increased in 2000 year. 3. The prevailing research design were the non-experimental design, quasi-experimental and pre-experimental designs in order. 4. The data collection method used most often physiologic measures(32.8%). 5. The domain of the key concepts that prevailed was health domain(51.8%), nursing domain(22.9%), and human being domain(20.5%) in order. And environmental domain (4.8%) were relatively low.

Comparison of accuracy of breeding value for cow from three methods in Hanwoo (Korean cattle) population

Hyo Sang Lee,Yeong Kuk Kim,Doo Ho Lee,Dongwon Seo,Dong Jae Lee,ChangHee Do,Phuong Thanh N. Dinh,Waruni Ekanayake,Kil Hwan Lee,Du-Hak Yoon,Seung Hwan Lee,Yang Mo Koo 한국축산학회 2023 한국축산학회지 Vol.65 No.4

In Korea, Korea Proven Bulls (KPN) program has been well-developed. Breeding and evaluation of cows are also an essential factor to increase earnings and genetic gain. This study aimed to evaluate the accuracy of cow breeding value by using three methods (pedigree index [PI], pedigree-based best linear unbiased prediction [PBLUP], and genomic-BLUP [GBLUP]). The reference population (n = 16,971) was used to estimate breeding values for 481 females as a test population. The accuracy of GBLUP was 0.63, 0.66, 0.62 and 0.63 for carcass weight (CWT), eye muscle area (EMA), back-fat thickness (BFT), and marbling score (MS), respectively. As for the PBLUP method, accuracy of prediction was 0.43 for CWT, 0.45 for EMA, 0.43 for MS, and 0.44 for BFT. Accuracy of PI method was the lowest (0.28 to 0.29 for carcass traits). The increase by approximate 20% in accuracy of GBLUP method than other methods could be because genomic information may explain Mendelian sampling error that pedigree information cannot detect. Bias can cause reducing accuracy of estimated breeding value (EBV) for selected animals. Regression coefficient between true breeding value (TBV) and GBLUP EBV, PBLUP EBV, and PI EBV were 0.78, 0.625, and 0.35, respectively for CWT. This showed that genomic EBV (GEBV) is less biased than PBLUP and PI EBV in this study. In addition, number of effective chromosome segments (Me) statistic that indicates the independent loci is one of the important factors affecting the accuracy of BLUP. The correlation between Me and the accuracy of GBLUP is related to the genetic relationship between reference and test population. The correlations between Me and accuracy were −0.74 in CWT, −0.75 in EMA, −0.73 in MS, and −0.75 in BF, which were strongly negative. These results proved that the estimation of genetic ability using genomic data is the most effective, and the smaller the Me, the higher the accuracy of EBV.

S-363 Chemical pleurodesis using mistletoe extracts via spray catheter during medical thoracoscopy

( Sun Hack Lee ),( Jung Seop Eom ),( Tae Hwa Kim ),( Kyu Min Lee ),( Ji Yeon Shin ),( Han Na Lee ),( Bo Hye Song ),( Yong Ki Sim ),( Geewon Lee ),( Jeong Ha Mok ),( Hyo Yeong Ahn ),( Min Ki Lee ) 대한내과학회 2016 대한내과학회 추계학술대회 Vol.2016 No.1

We present three cases of successful chemical pleurodesis with a liquid solution of mistletoe extract using a spray catheter during medical thoracoscopy. The medical thoracoscopy was performed in all presented cases to remove pleural effusion and conduct chemical pleurodesis to manage symptomatic malignant pleural effusion. A spray catheter was used to instill the mistletoe extract evenly into the pleural cavity, and there were no pleurodesis-related complications. Respiratory symptoms caused by pleural effusion improved after pleurodesis, and successful pleurodesis was maintained for more than 3 months after medical thoracoscopy in all three patients. Lee and Colt reported that instilling lidocaine via a spray catheter is effective for pain control before chemical pleurodesis during medical thoracoscopy. Using a spray catheter in the same way, we performed pleurodesis with a mistletoe extract via a spray catheter in three patients with MPE. A complete response, defined as no pleural effusion within 4 weeks after the last pleurodesis6, was achieved in all three patients. Several studies have used liquid mistletoe extract via a chest tube for chemical pleurodesis. Stumpf et al. reported that the overall response to mistletoe extract pleurodesis was 72% in 20 patients with MPE. Mistletoe extract, which was previously verified as an effective sclerosant for pleurodesis via a chest tube, could be evenly sprayed into the pleural cavity during thoracoscopy using a spray catheter. Distributing the sclerosant evenly into the pleural cavity with a spray catheter may lead to successful pleurodesis, particularly in patients who underwent medical thoracoscopy. In conclusion, We found that instilling the mistletoe extract using a spray catheter was an effective pleurodesis method and could possibly be performed as an alternative to talc poudrage.

Construction of a Steel Composite Cable-Stayed Bridge with Curved Pylon : The Handuri Bridge

Yeong-Dae Lee,Seo-Kyung Cho,Jun-Ho Lee,Hyo-Chan Kwon,Jae-Moon Kim,Do-Hyun Baek 한국강구조학회 2013 Steel Construction Vol.- No.5

Perception of Long-Term Oxygen Therapy for Chronic Lung Diseases May Affect Poor Adherence in Korea

( Hyo Jin Kim ),( Hongyeul Lee ),( Ji Young Yang ),( Jae Ha Lee ),( Seung Won Ra ),( Sungmin Hong ),( Ho Young Lee ),( Sung Hyun Kim ),( Mi-yeong Kim ),( Hyun-kyung Lee ) 대한결핵 및 호흡기학회 2024 Tuberculosis and Respiratory Diseases Vol.87 No.1

Background: Long-term oxygen therapy (LTOT) improves the survival of patients with hypoxemia due to chronic respiratory diseases. The clinical outcomes of LTOT are strongly associated with patient adherence. To improve the adherence of patients, physicians have focused on the efficacy of LTOT. However, poor adherence may stem from patients’ perceptions of LTOT. Herein we evaluated patients’ perceptions of LTOT affecting adherence. Methods: We conducted a cross-sectional survey study using descriptive, open, and closed-ended questionnaire. Patients using oxygen therapy (OT) or requiring it but avoiding OT responded to the questionnaires at three university hospitals. Results: Seventy-nine patients responded to the questionnaires. The number of patients using home and portable OT was 69 (93%) and 37 (46.3%), respectively. Patients with good adherence were 22 (30.1%). Among patients with good adherence, 90.9% used oxygen according to physicians’ prescriptions whereas only 37.3% of those with poor adherence followed physicians’ prescriptions (p<0.01). The reasons for avoiding using home OT were fear of permanent use (50%), unwanted attention (40%), and lack of symptoms (40%). They avoided portable OT because of unwanted attention (39%), heaviness (31.7%), and lack of symptoms (21.6%). Conclusion: Patients on LTOT had the perception of the misunderstanding the effects of OT and of psychosocial barriers to initiate or use LTOT. Considering these findings, health professionals need to provide effective education on the purpose of LTOT to improve patient adherence to OT and provide sufficient support for the management of psychosocial barriers in patients using LTOT.

Incidence and clinical characteristics of clonal cytogenetic abnormalities of acquired aplastic anemia in adults

Lee, Ji Hyun,Kwon, Kyung A,Lee, Suee,Oh, Sung Yong,Kim, Sung-Hyun,Kwon, Hyuk-Chan,Han, Jin-Yeong,Song, Moo-Kon,Chung, Joo-Seop,Lee, Ho Sup,Kim, Yang Soo,Lee, Sang-Min,Joo, Young-Don,Kim, Hyo-Jin Korean Society of Hematology; Korean Society of Bl 2010 Blood Research Vol.45 No.4

<P><B>Background</B></P><P>Cytogenetic abnormalities (CAs) have been reported frequently in patients with otherwise typical aplastic anemia (AA), but their implications in the prognosis and in the evolution to hematologic malignancies are controversial.</P><P><B>Methods</B></P><P>We retrospectively analyzed 127 adult AA patients who had successful cytogenetic analysis at initial diagnosis.</P><P><B>Results</B></P><P>The patients were classified into 3 groups according to the initial and follow-up results of cytogenetic profiles. Group 1 included patients who had persistent AA with normal cytogenetic profiles (N=117); Group 2, those who had a normal cytogenetic profile at initial diagnosis but later acquired CA (N=4, 3.1%); and Group 3, those who had CA at the initial diagnosis, regardless of follow-up cytogenetic status (N=6,4.7%). In Group 2, 2 patients later developed CA without progression to acute myeloid leukemia (AML) or myelodysplastic syndrome (MDS); the other 2 patients later progressed to AML. None of the patients in Group 3 progressed to AML or MDS. There was no significant difference in overall survival between Groups 1 and 3.</P><P><B>Conclusion</B></P><P>AA patients with CA at initial diagnosis or follow-up may not be at greater risk for evolution to AML or MDS, or show shorter survival periods. Prospective studies and a larger patient samples are needed to establish the clinical relevance of CA.</P>

Screening for α-Glucosidase and Adipocyte Differentiation Inhibition in Barleys

Hyo-Seon Ryu,Yongjin Lee,Mi Ja Lee,Ju Ri Ham,Ra-Yeong Choi,Kwang-Jin Kim,Young-Jin Son,Mi-Kyung Lee 한국식품영양과학회 2018 한국식품영양과학회 학술대회발표집 Vol.2018 No.10

Agreement of two ELISAs for Mycobacterium avium subspecies paratuberculosis in cattle in Korea

Lee, Kyung Woo,Jung, Byeong Yeal,Hwang, In Yeong,Lee, Su Hwa,Kim, Ji Yeon,Kim, Young Hoan,Lee, Seong Hyo,Moon, Oun Kyoung,Lee, O Soo The Korean Society of Veterinary Science 2009 大韓獸醫學會誌 Vol.49 No.2

Paratuberculosis caused by Mycobacterium avium subspecies paratuberculosis (Mpt) is a chronic infectious enteric disease with deleterious impact on the performance in ruminants. In Korea, ELISA has been introduced to detect antibodies to Mpt in individual cattle. However, comparison study with ELISA has not been studied until now. In total, a panel of 899 serum samples obtained from dairy cattle was analyzed with two commercial ELISAs for Mpt to assess the performance. Two ELISAs employed in this study were both licensed worldwide. Two ELISAs applied onto same serum samples showed the moderate agreement (kappa value = 0.60). There was non-significant McNemar test (p = 0.0614) between two ELISA results indicating that each proportion detected by two kits did not differ. In addition, the percent agreement between two ELISA results was turned out to be 96.8% which interpreted excellent reproducibility. It was shown from this study that two ELISAs revealed moderate kappa agreement performance. The implication raised is that when ELISAs as diagnostics are used to detect Mpt in individual cattle, positive reaction by either ELISA should be interpreted as serologically Mpt positive due to presumed low sensitivity of ELISAs and their test agreement being less than 100%.