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Ju Ri Ham,Yongjin Lee,Young-Jin Son,Ra-Yeong Choi,Mi-Kyung Lee 한국식품영양과학회 2021 한국식품영양과학회 학술대회발표집 Vol.2021 No.10
Edible insects are attracting attention as a sustainable food resource in future food. Recently, researches on the relationship between bone health and chronic diseases are increasing. Therefore, this study attempted to determine the anti-osteoporosis effect of Tenebrio Molitor larva fermentation extract (TM). The TRAP staining of bone marrow macrophage cells indicated that TM dose-dependently inhibited osteoclastogenesis compared with the vehicle. TM treatment down-regulated NFATc1, DC-STAMP, OSCAR, Ctsk, and Trap mRNA expression. NFATc1, a major protein in osteoporosis, level was decreased by the TM treatment. The ALP staining of C2C12 cells indicated that TM dose-dependently increased osteoblastogenesis. In high-fat fed mice, TM supplementation significantly increased femoral and tibial bone mineral density. These results demonstrate the anti-osteoporotic effects of TM by regulating osteocalstogenesis and osteoblastogenesis, which provided useful information for its application and development against obesity-induced osteoporosis.
Anti-obesity and anti-hepatosteatosis effects of dietary scopoletin in high-fat diet fed mice
Ham, Ju Ri,Lee, Hae-In,Choi, Ra-Yeong,Sim, Mi-Ok,Choi, Myung-Sook,Kwon, Eun-Young,Yun, Kyeong Won,Kim, Myung-Joo,Lee, Mi-Kyung Elsevier 2016 Journal of Functional Foods Vol.25 No.-
<P><B>Abstract</B></P> <P>The effects of scopoletin on non-alcoholic fatty liver in obese mice were investigated. Mice were fed high-fat diet (HF) with or without two doses of scopoletin (0.01 and 0.05%, w/w) for 16 weeks. Both doses of scopoletin led to similar reductions in body weight, visceral fat, serum levels of leptin, lipid, TNFα, IL-6, IFNγ and MCP-1, insulin resistance and hepatic lipid accumulation, whereas they increased serum adiponectin and faecal lipid levels. Ingenuity pathway analysis revealed that hepatic gene networks related to lipid concentrations, inflammation of organs, quantity of adipose tissue, proliferation of cell and necrosis were down-regulated in the scopoletin group. The top up- or down-regulated genes were <I>Cidea</I>, <I>Apoa4</I>, <I>Cyp7a1</I>, <I>Errfi1</I>, <I>Col1a1</I>, <I>Mmp13</I>, <I>Cdkn1a</I>, <I>Gdf15</I> and <I>Saa1</I>, which emerged as associated genes related to hepatic steatosis and inflammation. These results indicate that scopoletin may ameliorate HF-induced hepatic dysfunction via regulation of lipid metabolic and inflammatory genes.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Scopoletin attenuates non-alcoholic fatty liver disease in high-fat diet fed mice. </LI> <LI> Scopoletin identified 3 gene networks related to lipid metabolism and inflammation. </LI> <LI> Target genes of lipid metabolism are <I>Cidea</I>, <I>Apoa4</I>, <I>Cyp7a1</I> and <I>Errfi1</I>. </LI> <LI> Target genes of inflammation are <I>Col1a1</I>, <I>Mmp13</I>, <I>Cdkn1a</I> and <I>Saa1.</I> </LI> </UL> </P>
Heshouwu (Polygonum multiflorum Thunb.) Extract Attenuates Bone Loss in Diabetic Mice
Ju Ri Ham,Hae-In Lee,Ra-Yeong Choi,Hyo-Seon Ryu,Sung-Tae Yee,Kyung-Yun Kang,Mi-Kyung Lee 한국식품영양과학회 2019 Preventive Nutrition and Food Science Vol.24 No.2
This study investigated the effects and mechanism of Heshouwu (Polygonum multiflorum Thunb.) water extract (HSW) on diabetes-related bone loss in mice. HSW was orally administered (300 ㎎/㎏ body weight) to high-fat diet and streptozotocin-induced diabetic mice for 10 weeks. HSW significantly alleviated mouse body weight loss and hyperglycemia compared with the control group, and elevated serum levels of insulin, osteocalcin, and bone-alkaline phosphatase. HSW supplementation also significantly increased the bone volume/tissue volume ratio and trabecular thickness and number, and decreased the bone surface/bone volume ratio and trabecular structure model index in the femur and tibia. Moreover, HSW significantly increased femoral bone mineral density. In addition, HSW down-regulated osteoclastogenic genes, such as nuclear factor of activated T-cells, cytoplasmic 1 and tartrate-resistant acid phosphatase 5 (TRAP), in both the femur and tibia tissue, and reduced serum TRAP level compare to those of control mice. These results indicate that HSW might relieve diabetes-related bone disorders through regulating osteoclast-related genes, suggesting HSW may be used as a preventive agent for diabetes-induced bone loss.
Methoxsalen supplementation attenuates bone loss and inflammatory response in ovariectomized mice
Ham, Ju Ri,Choi, Ra-Yeong,Yee, Sung-Tae,Hwang, Yun-Ho,Kim, Myung-Joo,Lee, Mi-Kyung Elsevier 2017 Chemico-biological interactions Vol.278 No.-
<P><B>Abstract</B></P> <P>Methoxsalen (MTS) is a natural bioactive compound found in a variety of plants that has many known biofunctions; however, its effects on osteoporosis and related mechanisms are not clear. This study examined whether MTS exhibited preventive effects against postmenopausal osteoporosis. Female C3H/HeN mice were divided into four groups: Sham, ovariectomy (OVX), OVX with MTS (0.02% in diet), and OVX with estradiol (0.03 μg/day, s.c). After 6 weeks, MTS supplementation significantly increased femur bone mineral density and bone surface along with bone surface/total volume. MTS significantly elevated the levels of serum formation markers (estradiol, osteocalcin and bone-alkaline phosphatase) such as estradiol in OVX mice. Tartrate resistant acid phosphatase staining revealed that MTS suppressed osteoclast numbers and formation in femur tissues compared with the OVX group. Supplementation of MTS slightly up-regulated osteoblastogenesis-related genes (<I>Runx-2</I>, <I>osterix</I>, <I>osteocalcin</I>, and <I>Alp</I>) expression, whereas it significantly down-regulated inflammatory genes (<I>Nfκb</I> and <I>Il6</I>) expression in femur tissue compared with the OVX group. These results indicate that MTS supplementation effectively prevented OVX-induced osteoporosis via enhancement of bone formation and suppression of inflammatory response in OVX mice. Our study provides valid scientific information regarding the development and application of MTS as a food ingredient, a food supplement or an alternative agent for preventing postmenopausal osteoporosis.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Methoxsalen improves bone mineral density in ovariectomized mice. </LI> <LI> Methoxsalen increased serum bone-ALP, osteocalcin and estradiol levels. </LI> <LI> Methoxsalen down-regulated <I>Il6</I> and <I>Nfκb</I> gene expression in femur. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>
Anti-Inflammatory and Antioxidant in Vitro Activities of Magnoliae Flos Ethanol Extract
Ju Ri Ham,Kyeong Won Yun,Mi-Kyung Lee 한국식품영양과학회 2021 Preventive Nutrition and Food Science Vol.26 No.4
This study evaluated Magnoliea Flos ethanol extract (MFE) as a potential natural anti-inflammatory and antioxidant in lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophages and in vitro antioxidant assays. MFE (10, 30, and 50 μg/mL) dose-dependently inhibited LSP-induced nitric oxide production, which is mediated by down-regulating gene and protein expression of inducible nitric oxide synthase and cyclooxygenase-2. MFE also down-regulated both gene and protein expression of nuclear factor-kappa B and its downstream genes, such as tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6), compared with vehicle-treated cells. As a result, MFE treatment of LPS-stimulated macrophages significantly suppressed release of pro-inflammatory cytokines, such as TNF-α and IL-6. The antioxidant in vitro test revealed 2,2-diphenyl-1-picrylhydrazyl and 2,2-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) radical scavenging activities of MFE (0.25∼5 mg/mL) of 16.62% to 75.17% and 38.54% to 92.91%, respectively. The ferric reducing antioxidant ability of MFE was 0.54 mM to 2.14 mM. Overall, MFE exhibited antioxidant activity and an effective anti-inflammatory response in LPS-stimulated macrophages, which is potentially valuable for application as a natural functional material.
Ju Ri Ham,Mi Ja Lee,Hyun-Jin Lee,Young-Jin Son,Hae-In Lee,Mi-Kyung Lee 한국식품영양과학회 2021 한국식품영양과학회 학술대회발표집 Vol.2021 No.10
The antidiabetic property of β-glucan is well known. This study investigated the hypoglycemic effects of β-glucan (BG, 500 mg/kg, oral) concentrated from betaone barley and its underlying mechanism by comparing with metformin (Met, 400 mg/kg, oral) in a high-fat diet and streptozotocin-induced diabetic mice. After six weeks, BG and Met significantly lowered fasting blood glucose, hepatic triglyceride and free fatty acid level, while they increased pancreas insulin expression compared to the control group. BG improved glucose and insulin intolerances in diabetic mice. BG and Met down-regulated hepatic genes expression of gluconeogenesis-related (PEPCK and G6Pase), lipid synthesis-related (PAP and DGAT2), and inflammation-related (NFκB, TNFα, and IL6), whereas they up-regulated fatty acid β-oxidation-related genes (PGC1α and Acsl1) levels compared to the control group. Thus, β-glucan of betaone barley effectively lowered blood glucose in diabetic mice and alleviated fatty liver and inflammatory response by mechanism similar to metformin. These results suggest beta-glucan of barlery could be used as a potential preventive agent against diabetes.