들잔디 성숙종자로부터 캘러스배양 및 식물체 재분화에 미치는 몇 가지 요인의 영향

이상훈,김범수,원성혜,조진기,김기용,박근제,성병렬,이효신,이병현 Plant molecular biology and biotechnology research 2004 Plant molecular biology and biotechnology research Vol.2004 No.-

In an effort to optimize tissue culture responses of zoysiagrass (Zoysia japonica Steud.) for genetic transformation, factors affecting callus induction and plant regeneration were investigated. MS medium containing 3 ㎎/L 2,4-D was optimal for embryogenic callus induction from mature seed. The plant regeneration frequency of 73.3% was observed when embryogenic calli induced in this medium were transferred to N6 medium supplemented with 0.1 ㎎/L 2,4-D and 5 ㎎/L BA. Among several basic media, MS and N6 medium were optimal for callus induction and plant regeneration, respectively. Regenerated plants were grown normally when shoots transplanted to the soil. A rapid and efficient plant regeneration system established in this study will be useful for molecular breeding of turfgrass through genetic transformation.

돼지에서 정맥, 근육 그리고 경구 투여시의 enrofloxacin의 약물동태학

윤효인,김무열,박승춘,조준형,박병권,이내경,노상석,장범수,신광순,조명행 충남대학교 수의과대학 동물의과학연구소 1996 動物醫科學硏究誌 Vol.4 No.-

In order to characterize pharmacokinetic profiles according to route of a new enrofloxacin salt form (Enrotil®), it was given to 4 healthy pigs via oral (p.o.), intramuscular (i.m.) or intravenous (i.v.) administrations at a dose rate of 5 ㎎/㎏ body weight. Enrofloxacin (ENFX) in serum was detected by bioassay using E. coli BE1186 as a test organism. The biological elimination half-lives (t_1/2(β)) of ENFX were 6.76±0.99 h (i.v.), 7.16±2.30 h (i.m.) and 11.45±3.90 h (p.o.), Volume of distribution (Vd) of enrofloxacin was 2.20±0.31 L/㎏ (i.v.), 2.52±0.60 L/㎏ (i.m.) and 1.88±0.33 L/㎏ (i.m.). Mean residence time (MRT) was 8.77±1.26 h after i.v. injection and the maximal concentration time (Tmax) following p.o. and i.m. administration was 0.76±0.09 h and 0.60±0.12 h, indicating a rapid absorption from these routes. Bioavailibility (F) was calculated as 64.1% for p.o. administration and 59.71% for i.m. injections. In summary, the newly formulated enrofloxacin salt form has shown a high water solubility, rapid absorption and large tissue distribution, suggesting a potential antibacterials for oral application on a large scale in veterinary sectors.

간경변증 환자에서 Escherichia colli 균혈증 합병 시 간기능 장애에 따른 C-reactive protein 생성 능력에의 영향

박완범,강철인,김동민,이기덕,장희창,김흥빈,오명돈,이효석,최강원 대한감염학회 2003 감염과 화학요법 Vol.35 No.5

간경변증 환자에서 Escherichia coli 균혈증 합병 시 간기능 장애에 따른 C-reactive protein 생성 능력에의 영향

박완범,강철인,김동민,이기덕,장희창,김홍빈,오명돈,이효석,최강원 대한감염학회 2003 감염과 화학요법 Vol.35 No.5

목적 : C-reactive protein(CRP)은 간에서 생성되는 급성 반응물질이다. 하지만 간부전증 환자에서 CRP의 반응이 간기능에 따라 어느 정도 영향을 받는지는 별로 알려진 바가 없다. 본 연구에서는 간기능에 따른 CRP 생성 능력을 평가하고자 하였다. 방법 : E. coli 균혈증이 있는 간경변증 환자 30명을 대상으로 하였고 간기능은 균혈증이 발생하기 전 2개월 이내에 측정된 혈청 빌리루빈, 혈청 알부민, 프로트롬빈시간, Child-Pugh 점수로 평가하였다. 대조군 A는 간질환이 없으면서 E. coli 균혈증이 발생한 환자 30명으로 하였고 대조군 B는 간경변증이 있으면서 급성 감염의 증거가 없는 환자 30명으로 하였다. 환자군과 대조군 간에 CRP의 최대값을 비교하였다. 결과 : CRP의 최대값은 환자군에서 7.3±5.0㎎/dL, 대조군 A에서 17.9±8.3㎎/dL로 환자군에서 유의하게 낮았다.(P<0.001). 간경변증 환자에서 CRP의 생성은 Child-Pugh 점수에 비례하여 감소하였으나(P=0.004) Child=Pugh class C의 간기능을 가진 환자군에서도 대조군 B와 비교하여 의미있는 CRP의 생성을 보였다(5.3±3.2 vs. 0.5±0.4㎎/dL, P<0.001). 결론 : 간기능부전 환자에서 CRP 반응은 간기능 저하 정도에 따라 둔화되지만 심한 간기능 장애를 가진 환자에서도 CRP의 생성은 유지된다. Background : C-reactive protein (CRP) is an acute phase reactant produced in the liver. To assess the influence of liver dysfunction on the production of CRP, we evaluated CRP response to E. coli bacteremia in patients with or without liver cirrhosis (LC). Methods : 30 LC patients who developed spontaneous peritonitis with E. coli bacteremia were enrolled in the study. Baseline values of total bilirubin, serum albumin, and prothrombin time were obtained within 2 months prior to infection. Liver dysfunction was categorized according to the Child-Pugh score. 30 patients with E. coli bacteremia who had no underlying liver dysfunction were included as a control group. Matched-control of 30 LC patients without evidence of acute infection was also included. The peak CRP values were compared among the groups. Results : In the patients with E. coli bacteremia, the mean value of peak CRP was 7.3 (+/- 5.0) ㎎/dL in LC patients, 17.9 (+/- 8.3) mg/dL in patients without liver dysfunction (p<0.001). In the advanced LC patients with Child-Pugh class C, the level of CRP was 5.2 (+/- 3.3) ㎎/dL in patients with E. coli bacteremia, 0.5 (+/- 0.4) ㎎/dL in patients without acute infection (P<0.001). Child-Pugh score had correlation with decrease of CRP (linear regression test, P=0.004). Conclusion : CRP response during E. coli bacteremia was attenuated but maintained even in patients with advanced liver dysfunction.

토끼에서 근육주사시 입자 크기에 따른 amoxicillin의 비교 약물동태학

박승춘,윤효인,오태광,장범수,배순이,조준형,정상희,이내경,김민규 충남대학교 수의과대학 동물의과학연구소 1998 動物醫科學硏究誌 Vol.6 No.-

To investigate the pharmacokinetic difference between the two amoxicillin (AMX) particles in rabbits after intramuscular injection (i.m.), both of AMX-S (particle size: 10 ㎍) and AMX-L (particle size: 100 ㎍) were injected into New Zealand White rabbits (1.2±0.3 ㎏) at a dose rate of 10 ㎎/㎏ of body weight. In this study, serum AMX concentrations were detected by microbiological assay with E. coli BE 1186 which shows high antibiotic sensitivity. After i.m. administration, AMX-S and AMX-L were best fitted as 1-compartment model with the absorption and elimination phase. The biological half-life (T_1/2, _k10) of AMX-S is 4.06±1.09 h and that of AMX-L 4.76±0.69 h. The serum maximal concentration time (T_max) of AMX-S and AMX-L were 0.88±0.17 h and 0.77±0.11 h, respectively. Maximal AMX concentration (C_max) (AMX-S: 5.71±0.62 ㎍/㎖, AMX-L: 5.04±0.25 ㎍/㎖) in serum showed a significant difference (p<0.05). In terms of bioequivalance, however, there was no difference between the two AMX's after i.m. injection in the basis of C_max and AUC.

Vegetative Growth of Young Phalaenopsis ‘Hwasu 355’ and ‘Hwasu 3551’ Plants Treated with FourRelatively Low Temperature

Hyo Beom Lee,Ki Sun Kim 한국원예학회 2015 한국원예학회 학술발표요지 Vol.2015 No.5

Prediction of Treatment Response in Patients with Chronic Obstructive Pulmonary Disease by Determination of Airway Dimensions with Baseline Computed Tomography

Hyo Jung Park,Sang Min Lee,최주애,Sang Min Lee,Namkug Kim,Jae Seung Lee,Yeon-Mok Oh,Joon Beom Seo 대한영상의학회 2019 Korean Journal of Radiology Vol.20 No.2

Objective: To determine the predictive factors for treatment responsiveness in patients with chronic obstructive pulmonary disease (COPD) at 1-year follow-up by performing quantitative analyses of baseline CT scans. Materials and Methods: COPD patients (n = 226; 212 men, 14 women) were recruited from the Korean Obstructive Lung Disease cohort. Patients received a combination of inhaled long-acting beta-agonists and corticosteroids twice daily for 3 months and subsequently received medications according to the practicing clinician’s decision. The emphysema index, airtrapping indices, and airway parameter (Pi10), calculated using both full-width-half-maximum and integral-based half-band (IBHB) methods, were obtained with baseline CT scans. Clinically meaningful treatment response was defined as an absolute increase of ≥ 0.225 L in the forced expiratory volume in 1 second (FEV1) at the one-year follow-up. Multivariate logistic regression analysis was performed to investigate the predictors of an increase in FEV1, and receiver operating characteristic (ROC) analysis was performed to evaluate the performance of the suggested models. Results: Treatment response was noted in 47 patients (20.8%). The mean FEV1 increase in responders was 0.36 ± 0.10 L. On univariate analysis, the air-trapping index (ATI) obtained by the subtraction method, ATI of the emphysematous area, and IBHB-measured Pi10 parameter differed significantly between treatment responders and non-responders (p = 0.048, 0.042, and 0.002, respectively). Multivariate analysis revealed that the IBHB-measured Pi10 was the only independent variable predictive of an FEV1 increase (p = 0.003). The adjusted odds ratio was 1.787 (95% confidence interval: 1.220–2.619). The area under the ROC curve was 0.641. Conclusion: Measurement of standardized airway dimensions on baseline CT by using a recently validated quantification method can predict treatment responsiveness in COPD patients.

Growth and Inflorescence Initiation of Phalaenopsis Orchids by Paclobutrazol Treatment

Hyo Beom Lee,Ki Sun Kim 한국화훼학회 2020 화훼연구 Vol.28 No.4

본 연구는 paclobutrazol(PBZ) 처리에 따른 호접란의 생장 반응과 화경유도를 확인하기 위해 수행되었다. 20°C 생식생 장 환경에서 호접란 ‘Mantefon’과 ‘V3’ 실험묘를 0, 100, 200, 400ppm(유효성분 0, 1, 2, 4mg) PBZ 농도로 처리하였고, 28°C 영양생장 환경에서 ‘Mantefon’ 실험묘를 0, 100, 200ppm(유효성 분 0, 1, 2mg) PBZ 농도로 처리하였다. 화경유도 환경에서는 200, 400ppm 처리 식물들의 화경유도율이 감소하고 화경의 출 현이 늦어지는 것을 관찰하였다. 또한 ‘Mantefon’에서 처리 농도 에 상관없이 모든 PBZ 처리구에서 화경의 수가 감소하였다. PBZ 처리는 화경의 길이도 감소시켰으나 개화소요일수나 소화 의 수에는 차이가 없었다. 한편, 영양생장 환경에서는 처리 농도 가 증가할수록 줄기와 뿌리 직경, 잎의 두께는 증가하였고, 반면 에 신엽의 수, 잎의 길이와 폭은 감소하였다. 따라서 PBZ 처리를 받은 식물에서 ‘stunting’ 현상이 나타났다. 영양생장 환경에서 PBZ 처리를 받은 식물을 생식생장 환경으로 옮겼을 때도 앞선 실험에서와 마찬가지로 화경유도율이 감소하고 화경출현일수 가 증가하여서 영양생장 동안의 PBZ 처리 효과가 화경유도에까 지 이어질 수 있다는 것을 확인하였다. 이러한 결과를 통해 PBZ 처리는 호접란의 생장과 화경유도를 제한할 수 있다는 것을 알 수 있었다. The effects of paclobutrazol (PBZ) treatment on growth and inflorescence initiation were investigated in Phalaenopsis orchids. Clones of the Phalaenopsis cultivars Queen Beer ‘Mantefon’ and Sogo Yukidian ‘V3’ were treated with 0, 100, 200, and 400 ppm (a.i. 0, 1, 2, and 4 mg) PBZ at 20°C during the forcing period and 0, 100, and 200 ppm (a.i. 0, 1, and 2 mg) PBZ at 28°C during the vegetative period by drenching. Under forcing condition, the percent plants with visible inflorescence (VI, flower-stalk longer than 0.5 cm) was significantly reduced by 200 and 400 ppm PBZ treatments, and inflorescence emergence was delayed in all PBZ treatments. The number of inflorescences decreased in ‘Mantefon’ by PBZ treatment regardless of concentrations. The PBZ treatments shortened the inflorescence length, but there was no difference in the number of days to bloom and the number of flowers. Under vegetative condition, PBZ treatment significantly increased the stem and root diameters and leaf thickness with increasing PBZ concentration, whereas it decreased the number of new leaves and leaf length and width. Thus, the PBZ-treated plants seemed to be stunted. When the plants pre-treated with PBZ during the vegetative period were moved to forcing conditions, the percent plants with VI significantly decreased and the number of days to VI increased, implying that the effects of the PBZ treatment during the vegetative period could persist in inflorescence initiation. From these results, we conclude that PBZ treatment can inhibit the growth and inflorescence initiation in Phalaenopsis orchids.

Diagnostic performance of breast-specific gamma imaging in the assessment of residual tumor after neoadjuvant chemotherapy in breast cancer patients

Lee, Hyo Sang,Ko, Beom Seok,Ahn, Sei Hyun,Son, Byung Ho,Lee, Jong Won,Kim, Hee Jeong,Yu, Jong Han,Kim, Sung-Bae,Jung, Kyung Hae,Ahn, Jin-Hee,Cha, Joo Hee,Kim, Hak Hee,Lee, Hee Jin,Song, In-Hye,Gong, G Springer-Verlag 2014 Breast cancer research and treatment Vol.145 No.1

Structures and N→Si Bond Characters of 1-Fluorosilatrane and the Silatranyl Cation

Lee, Hyo-Sug,Bae, Cheol-Beom,Do, Young-Kyu,Lee, Yoon-Sup Korean Chemical Society 2002 Bulletin of the Korean Chemical Society Vol.23 No.2

The structures of 1-fluorosilatrane and the silatranyl cation were calculated by Hartree-Fock (HF), Mofller-Plesset second order (MP2), and various density functional theory (DFT) methods using many different basis sets, demonstrating that the Si-N bonds in two species are quite different. The N${\rightarrow}$Si bond distance of 1-fluorosilatrane from the hybrid DFT calculations $({\sim}2.32{\AA})$ using the Perdew-Wang correlation functional agrees with the gas phase experimental value $(2.324{\AA})$, while other functionals yield larger distances. The MP2 bond distance (2.287${\AA}$ with 6-311$G^{\ast}$) is shorter, and the HF one (2.544 ${\AA}$ with 6-311$G^{\ast}$) larger than those of DFT calculations. The MP2 bond distance is in good agreement with experiment indicating that the electron correlations are crucial for the correct description of the N${\rightarrow}$Si interaction. The silatranyl cation is a stable local minimum on the potential energy surface in all methods employed suggesting that the cation could be a reaction intermediate. The Si-N bond length for the cation is about 1.87 ${\AA}$ for all calculations tested implying that the Si-N bond is mainly conventional. Bonding characteristics of the Si-N bond in two species derived from the natural bond orbital analysis support the above argument based on calculated bond lengths.