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Transcriptome profiling of olive flounder responses under acute and chronic heat stress
Woo‑Jin Kim,이규빈,Dain Lee,Hyun‑Chul Kim,Bo‑Hye Nam,Hyungtaek Jung,이선주,Kyunghwan Kim 한국유전학회 2021 Genes & Genomics Vol.43 No.2
Background The olive founder (Paralichthys olivaceus) is a saltwater fsh, which is valuable to the economy. The olive founder strives to adapt to environmental stressors through physiological, biochemical, and transcriptional responses. The rise in water temperature threatens the growth, development, reproduction, and survival of olive founder. Each organ in the olive founder can diferentially respond to heat stress. Objectives The purpose of this study is to investigate organ-specifc transcriptional changes in olive founder tissues during heat stress. Methods In this study, transcriptome dynamics of the gill, liver, and muscle of olive founder to acute or chronic heat stress were investigated. Results Principal component analysis plotting revealed that the transcriptome of each organ is quite separated. K-means clustering, gene ontology, and Kyoto Encyclopedia of Genes and Genomes pathway analysis showed the diferential transcriptome responses of each organ to heat stress. Heat stress commonly afects the pathways involved in the correct protein folding, DNA repair, and cell cycle. Conclusion Our results may provide a valuable molecular basis of heat acclimation in fshes.
이탈리안 라이그래스의 성숙종자 유래 캘러스로부터 효율적인 식물체 재분화
우현숙,이상훈,이동기,김진수,원성혜,이병현 Plant molecular biology and biotechnology research 2004 Plant molecular biology and biotechnology research Vol.2004 No.-
As an initial step for future genetic manipulations to improve forage characteristics of Italian ryegrass (Lolium multiflorum Lam.), an efficient tissue culture system was established and the factors affecting plant regeneration were evaluated. MS medium containing 5 ㎎/L 2,4-D was optimal for embryogenic callus induction from mature seed and had a strong effect on successive plant regeneration. The plant regeneration frequency was observed at above 70% when embryogenic calli induced were transferred to N6 medium supplemented with 1 ㎎/L 2,4-D and 5 ㎎/L BA. Among several basic media tested, MS and M6 medium were optimal for callus induction and plant regeneration, respectively. Genotype was an important factor in plant regenerability. 'Jeanne' showed the highest regeneration frequency of 73%. A short tissue culture period and high-frequency regeneration system established in this study will be useful for molecular breeding of Italian ryegrass through genetic transformation.
새로운 Baculoviurs 전이벡터를 이용한 Escherichia coli β-galactosidase 유전자의 발현
우수동,김우진,김혜성,진병래,강석권 한국미생물생명공학회 ( 구 한국산업미생물학회 ) 1996 한국미생물·생명공학회지 Vol.24 No.1
국내에서 분리된 BmNPV를 이용하여 제작된 새로운 전이벡터 pBmKSK1에 외래 유전자로서 E. coli lacZ 유전자를 클로닝하고 재조합 바이러스를 제작하였다. 재조합 바이러스에 대하여 Southern blotting 분석을 실시하여 lacZ 유전자의 존재를 확인하였으며, 재조합 바이러스가 접종된 세포의 SDS-PAGE 및 Western blotting 분석을 통하여 β-galactosidase의 발현을 확인하였다. 재조합 바이러스의 발현효율을 누에 세포주 및 유충에서 비교 조사한 결과, 유충에서 더욱 높은 발현량을 보임을 확인하였다. To investigate the expression efficiency of new transfer vector of Bombyx mori nuclear polyhedrosis virus (BmNPV), Escherichia coli lacZ gene was inserted into new transfer vector pBmKSK1, under the control of polyhedrin promoter and expressed in BmN-4 cells coninfected with transfer vector pBmKSK1-LacZ and vild type BmNPV genome, and analysed by Southern blotting. The expression of β-galactosidase was characterized by SDS-PAGE, Western blotting and β-galactosidase activity assay. The results showed that the level of expression in silkworn larvae was higher than that of BmN-4 cells.
내부 응력이 .06 taper Profile의 피로 파절에 미치는 영향
정혜림,김진우,조경모,박세희 大韓齒科保存學會 2012 Restorative Dentistry & Endodontics Vol.37 No.2
Objectives: The purpose of this study was to evaluatie the reation between intentionally induced internal stress and cyclic fatigue failure of .06 taper ProFile. Materials and Methods: Length 25 mm, .06 taper ProFile (Dentsply Maillefer), and size 20, 25, 30, 35, and 40 were used in this study. to give the internal stress, the rotary NiTi files were put into the .02 taper, Endo-Training Bloc (Dentsply Maillefer) until auto-stop by torque controlled motor. Rotary NiTi files were grouped by the number of induced internal stress and randomly distributed among one control group and three experimental groups (n = 10, Stress 0 [control], Stress 1, Stress 2 and Stress 3). For cyclic fatigue measurement, time for separation of the rotary NiTi files was recorded. the fractured surfaces were observed by field emission scanning electron microscope (FE-SEM, SU-70, Hitachi). The tiem for separation was statistically analyzed using two-way ANOVA and post-hoc Scheffe test at 95% level. Results: In .06 taper ProFile size 20, 25, 30, 35, and 40, there were statistically significant difference on time for separation between control group and the other groups (p < 0.05). Conclustion: In the limitation of this study, cyclic fatigue failure of .06 taper ProFile is influenced by internal stress accumulated in the files. (Restor Dent Endod 2012;37(2):79-83)
국내 의료종사자에서 HHV-8의 항체양성률에 대한 연구
주우철,최용준,박재은,이혜명,이진수,정문현,김수미,문지혜 대한감염학회 2008 감염과 화학요법 Vol.40 No.4
HHV-8 virus is known to be associated with Kaposi's sarcoma (KS) in HIV seropositive patients and its seroprevalence has geographic difference, In South Korea, incidence of KS is low not only in general population but also in HIV patients compared to other countries, It is speculated that low seroprevalence of HHV-8 has contributed to the low incidence of KS in South Korea. We examined the seroprevalence of anti HHV-8 IgG of HIV seronegative health care workers in one University hospital. Enzyme-linked immunosorbent assay(ELISA) was used for diagnosis. One person (0.6% 1/164) was seropositive, six were equivocal (3.7%,6/164) and 157 people were seronegative (95.73%, 157/164). This study revealed that seroprevalence of HHV-8 in general population was. low in South Korea. Further studies are needed to be carried out to evaluate the low HHV-8 seroprevalence in Korea.
경유돌공 부위에서 전기신경자극기를 이용한 안면경련 환자의 치료
정창우,민진혜,이규탁 관동대학교 의과학연구소 2000 關東醫大學術誌 Vol.4 No.1
Facial spasm is the most common in middle aged women, which is a distressing condition characterized by involuntary, intermittent, unilateral twitching of the muscles innervated by the facial nerve. There are various treatments for facial spam, such as neurovascular decompression, local injection of botulinum, facial nerve compression at stylomastoid foramen and facial nerve block with O'Brien method. There are the same clinical problems associated with the reported procedure in pain clinic, such as severe pain and recurrence of spasm. In this paper, we report the modified facial nerve block using electric nerve stimulator at the stylomastoid foramen. This new technique will be able to reduce the pain and the discomfort during facial nerve block, and reduced the recurrence of facial spasm.
신혜진,김진우,조경모 大韓齒科保存學會 2003 Restorative Dentistry & Endodontics Vol.28 No.2
The purpose of this study is to evaluate the polymerization ability of three different light sources by microhardness test. Stainless steel molds of 1, 2, 3, 4 and 5 mm in thickness of 7 mm in diameter were prepared. The hybrid composite Z100 was packed into the hole of the mold and curing light was activated for designated time. Three different light sources, conventional halogen, light emitting diode, and plasma arc, were used for curing of composite. Two different curing times applied; one is to follow the manufacturers recommendation and the other is to extend the curing time of LED and plasma arc for balancing the light energy with halogen. Immediately after curing, the Vickers hardness was measured at the bottom of specimen. The results were as follows. 1. The composite cured with LED showed equal to higher microhardness than halogen. 2. The composite was cured with plasma arc by manufacturers recommendation showed lowest microhardness at all thickness. However, when curing time was extended, microhardness was higher than the others. In conclusion, this study suggested that plasma arc needs properly extended curing time.
원예치료를 통한 정신분열증 환자와 일반인들의 우울성 비교
이혜진,안창연,심우경 한국식물인간환경학회 2000 인간식물환경학회지 Vol.3 No.4
The purpose of this study was to investigate the change in depression for schizophrenia patient and people between pre- and post- horticultural therapy program. The result was as the follows ; Depression of schizophrenia patients averaged 61.83 to 51.33 and general people averaged 41.05 to 38.95(p〈.05). The result indicated that horticultural therapy program was effective in depression treatment not only schizophrenia patient but also general people.