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Oh, Jae‐,Hyeon,Ha, In Jin,Lee, Min Young,Kim, Eun‐,Ok,Park, Dain,Lee, Jun‐,Hee,Lee, Seok‐,Geun,Kim, Do‐,Wan,Lee, Tae‐,Ho,Lee, Eui‐,Ju,Kim, Chang‐,Kug John Wiley and Sons Inc. 2018 Journal of Separation Science Vol.41 No.12
<P><B>Abstract</B></P><P><I>Papaver</I> plants can produce diverse bioactive alkaloids. <I>Papaver rhoeas</I> Linnaeus (common poppy or corn poppy) is an annual flowering medicinal plant used for treating cough, sleep disorder, and as a sedative, pain reliever, and food. It contains various powerful alkaloids like rhoeadine, benzylisoquinoline, and proaporphine. To investigate and identify alkaloids in the aerial parts of <I>P. rhoeas</I>, samples were collected at different growth stages and analyzed using liquid chromatography coupled with quadrupole time‐of‐flight tandem mass spectrometry. A liquid chromatography with mass spectrometry method was developed for the identification and metabolite profiling of alkaloids for <I>P. rhoeas</I> by comparing with <I>Papaver somniferum</I>. Eighteen alkaloids involved in benzylisoquinoline alkaloid biosynthesis were used to optimize the liquid chromatography gradient and mass spectrometry conditions. Fifty‐five alkaloids, including protoberberine, benzylisoquinoline, aporphine, benzophenanthridine, and rhoeadine‐type alkaloids, were identified authentically or tentatively by liquid chromatography coupled with quadrupole time‐of‐flight tandem mass spectrometry in samples taken during various growth stages. Rhoeadine alkaloids were observed only in <I>P. rhoeas</I> samples, and codeine and morphine were tentatively identified in <I>P. somniferum</I>. The liquid chromatography coupled with quadrupole time‐of‐flight tandem mass spectrometry method can be a powerful tool for the identification of diverse metabolites in the genus <I>Papaver</I>. These results may help understand the biosynthesis of alkaloids in <I>P. rhoeas</I> and evaluate the quality of this plant for possible medicinal applications.</P>
Lee Dain,Kwon Sungmoon,Kim Hyoungkwang,Lee Ju Young,Ryu Byung-Han 대한감염학회 2023 Infection and Chemotherapy Vol.55 No.1
Multisystem inflammatory syndrome in adults (MIS-A) is a rare sequelae after coronavirus disease 2019 (COVID-19) that is characterized by fever as well as cardiovascular and gastrointestinal disorders. We present the case of an 80-year-old Korean woman with MIS-A who experienced febrile sensations, dyspnea, and whole body pain for 7 weeks after being diagnosed with COVID-19. Initial evaluation revealed heart failure, left pleurisy, and sensory neuropathy, but no evidence of infectious diseases was found. Her symptoms improved quickly after starting systemic glucocorticoid therapy, and inflammatory marker levels decreased. When treating patients with fever after COVID-19, it is critical to suspect MIS-A as one of the differential diagnoses for timely diagnosis and treatment.
Lee, Dain,Lee, Chaeyoung Springer 2015 Age Vol.37 No.2
<P>Age-related effects are often included as covariates in the analytical model for genome-wide association analysis of quantitative traits reflecting human health. Nevertheless, previous studies have hardly examined the effects of age on the proportion of variation explained by single nucleotide polymorphisms (PVSNP) in these traits. In this study, the PVSNP estimates of body mass index (BMI), waist-to-hip ratio, pulse pressure, high-density lipoprotein cholesterol level, triglyceride level (TG), low-density lipoprotein cholesterol level, and glucose level were obtained from Korean consortium metadata partitioned by gender or by age. Restricted maximum likelihood estimates of the PVSNP were obtained in a mixed model framework. Previous studies using pedigree data suggested possible differential heritability of certain traits with regard to gender, which we observed in our current study (BMI and TG; P?<?0.05). However, the PVSNP analysis based on age revealed that, with respect to every trait tested, individuals aged 40 to 49 exhibited significantly lower PVSNP estimates than individuals aged 50 to 59 or 60 to 69 (P?<?0.05). The consistent heterogeneous PVSNP with respect to age may be due to degenerated genetic functions in individuals between the ages of 50 and 69. Our results suggest the genetic mechanism of age- and gender-dependent PVSNP of quantitative traits related to human health should be further examined.</P>
Dain KIM,Jiyoung LEE,Taeeun LEE,Eunjung KIM 한국생물공학회 2021 한국생물공학회 학술대회 Vol.2021 No.10
MicroRNAs (miRNAs) are single-stranded molecules that play an important role in regulating gene expression in biological processes. The change in their expression is associated with various diseases, making them promising as a diagnostic biomarker. However, miRNAs are challenging to detect without amplification due to their short length and low abundance in biological fluids. Therefore, we developed an isothermal rolling circle amplification method to detect cancer-associated miRNAs, here miR-21. The dumbbell template was prepared by treating an annealed linear template with T4 DNA ligase and exonuclease I/III. Once the target recognized their toehold sequences, the dumbbell structure was changed through the strand displacement process, leading to the formation of circular template DNA. Isothermal amplification selectively occurred by a phi29 DNA polymerase, and the resulting amplicon was detected in real-time using an SYBR green dye. Our detection system showed a picomolar level of sensitivity at 30 minutes under optimized conditions, and its sensitivity can be further enhanced by generating short DNA strands continuously using a nicking enzyme. Thus, our platform presents a promising molecular diagnostic system for rapid and sensitive detection of specific miRNA.
Lee, Dain,Kim, Seongchan,Kim, Yeontae,Cho, Jeong Ho American Chemical Society 2017 ACS APPLIED MATERIALS & INTERFACES Vol.9 No.31
<P>Taking advantage of the superlative optoelectronic properties of single-layer MoS2, we developed a one-transistor one-transistor (1T1T)-type MoS2 optoelectronic nonvolatile memory cell. The 1T1T memory cell consisted of a control transistor (CT) and a memory transistor (MT), in which the drain electrode of the MT was connected electrically to the gate electrode of the CT, whereas the source electrode of the CT was connected electrically to the gate electrode of the MT. Single-layer MoS2 films were utilized as the channel materials in both transistors, and gold nanoparticles acted as the floating gates in the MT. This 1T1T device architecture allowed for a nondestructive read-out operation in the memory because the writing (programming or erasing) and read-out processes were operated separately. The switching of the CT could be controlled by light illumination as well as the applied gate voltage due to the strong light absorption induced by the direct band gap of single-layer MoS2 (similar to 1.8 eV). The resulting MoS2 1T1T memory cell exhibited excellent memory performance, including a large programming/erasing current ratio (over 10(6)), multilevel data storage (over 6 levels), cyclic endurance (200 cycles), and stable retention (10(3) s).</P>
Black phosphorus nonvolatile transistor memory
Lee, Dain,Choi, Yongsuk,Hwang, Euyheon,Kang, Moon Sung,Lee, Seungwoo,Cho, Jeong Ho The Royal Society of Chemistry 2016 Nanoscale Vol.8 No.17
<P>We demonstrated nanofloating gate transistor memory devices (NFGTMs) using mechanically-exfoliated few-layered black phosphorus (BP) channels and gold nanoparticle (AuNPs) charge trapping layers. The resulting BP-NFGTMs exhibited excellent memory performances, including the five-level data storage, large memory window (58.2 V), stable retention (10(4) s), and cyclic endurance (1000 cycles).</P>