애기마름(Trapa pseudoincisa S. et. Z.)으로부터 sterol 화합물의 분리 및 구조동정

양혜정,송명종,노영덕,김대근,백남인 경희대학교식량자원개발연구소 2007 硏究論文集 Vol.26 No.1

애기마름을 80% MeOH로 추출하고, 얻어진 추출물을 EtOAc, n-BuOH 및 H_(2)O로 용매 분획 하였다. EtOAc 분획으로부터 silica gel과 octadecyl silica gel(ODS) column chromatography를 반복 실시하여 2종의 화합물을 분리하였다. 각 화합물의 화학 구조는 NMR, MS 및 IR 등의 스펙트럼을 데이터를 해석하여, stigmasterol과 stigmasterol 3-O-β-D-glucopyranosyl로 구조 동정하였다. 이화합물은 애기마름에서는 처음 분리되었다. Trapa pseudoincisa was extracted with 80% aqueous MeOH, and the concentrated extract was partitioned with EtOAc, n-BuOH and H_(2)O. Two compounds were isolated from the EtOAc fraction through repeated silica gel and ODS column chromatographies. From the result of spectral data including NMR, MS, IR and GC/MS the chemical structures of the compounds were determined as stigmasterol (compound Ⅰ), and stigmasterol 3-O-β-D-glucopyranosyl (compound Ⅱ). They were isolated from Trapa pseudoincisa for the first time.

NOD/SCID 마우스 모델을 이용한 인간 제대혈 혼합이식에서의 생착 양상 분석

정양조,김동욱,조빈,강영주,박보배,김혜정,김태규,오일환 대한조혈모세포이식학회 2002 대한조혈모세포이식학회지 Vol.7 No.2

연구배경: 제대혈은 골수에 비해 많은 장점을 가지고 있음에도, 이식 후 성적에 있어 총세포수에 의해 좌우되며, 한 개의 태반으로부터 얻을 수 있는 총세포수의 한계가 있어 주로 소아에 국한되고 있다. 따라서 제대혈 응용의 확대를 위해 생착량을 늘이기 위한 방법으로 다중공여자에 의한 제대혈 혼합이식을 늘일 수 있는지 연구하기 위하여, 본 연구에서는 주조직적합성 형별로 조합된 제대혈을 NOD/ SCID 생쥐에 이식하는 방법으로, 혼합생착의 가능성을 연구하였다. 방법: 조합된 2개 공여자로부터 얻어진 제대혈들을 단일이식 및 혼합이식하여, 혼합이식된 숙주에 생착된 세포를 PCR-SSOP 방법을 사용하여 공여자 별로 추적하였다. 또한 각 공여자세포의 상대적 생착비율을 대조군인 단일제대혈 이식에서 얻어진 생착량과 비교하는 정량적 비교도 함께 하였다. 결과: 총단핵구를 혼합이식한 실험군에서는 주조직적합성의 일치도에 관계없이 한쪽공여자의 세포가 다른쪽에 비해 우세한 편향생착을 보였다. 그러나 임파구를 비롯한 Lineage 양성세포를 제거한 실험군에서는 주조직적합성의 불일치에도 불구하고 뚜렸한 생착의 공존이 관찰되었다. 결론: 본 연구는 제대혈의 생착량을 증가를 목적으로 총단핵구를 혼합이식할 경우 한쪽공여자의 세포에 의해 편중되며, 이것을 이식 후 생착과정에서의 두 종류의 세포의 경쟁에 의해 초래된다는 것을 보이고 있다. 또한 이러한 경쟁은 제대혈에 포함되어 있던 임파구들에 의한 것이며, 생착공존을 위하여는 공여자간 주조직적합성의 일치도 보다, 임파구의 제거가 더 중요하다는 것을 보여주고 있다. Background: Although umbilical cord blood (UCB) has been an attractive alternatives for bone marrow cells, application is limited to pediatric case due to limitation in total number of nucleated cells, that is a single most important factor for outcomes of UCB transplantation. Therefore, increasing overall engraftment by mixed transplantation of UCB derived from multiple donor should comprise one strategy to circumvent the posed limitation of UCB transplantation. In order to investigate the feasibility of establishing co-engraftment by multi-donor UCB cells, we carried out a xenotransaplantation study using NOD/SCID mice for systemic analysis of results on the input-based control of single unit transplantation. Methods: UCB units with various extent of HLA-matchings were co-transplanted into NOD/SCID mice along with single unit transplantation control. The relative contributions of engraftment by cells from each donor-derived were analyzed by HLA polymorphism using PCR-SSOP. Results: In all HLA-based mixed transplantation of total nucleated UCB cells, engraftment of one donor predominated over the other despite that equivalent amount of engraftments were achieved by single donor transplantation. When lineage depleted UCB units were co-transplanted, significant degree of co-engraftments were observed regardless of HLA disparity. Conclusion: Our result show that one donor cells dominate over the other in mixed UCB transplantation and that it is due to competition between donor cells during post-transplantation process. Our results suggest that immune cells contained in UCB unit mediate such competitioin and that 6 locus HLA matching would not be sufficient to prevent the competition.

피칸(Carya illinoensis C. KocH)으로부터 지질대사물질의 분리 및 동정

송명종,한민우,양혜정,이대영,노영덕,백남인 경희대학교식량자원개발연구소 2007 硏究論文集 Vol.26 No.1

피칸(Carya illinoensis C. Koch) 열매를 80% MeOH로 추출하고, 추출물을 극성에 따라 n-hexane, CHCl_(3), EtOAc, n-BuOH 및 물로 분배, 추출하였다. 이 중 EtOAc분획으로부터 silica gel column chromatography를 반복하여 4종의 지질화합물을 분리하였다. 분리된 화합물 화학구조는, NMR 및 MS 등의 스펙트럼을 해석하여 oleic acid methyl ester, linoleic acid methyl ester, β-sitosterol, daucosterol(3-O-β-D-glucopyaranosyl β-sitosterol)으로 동정하였다. Carya illinoensis C. Koch was extracted with 80% aqueous MeOH, and the concentrated extract was partitioned with n-hexane, CHCl_(3), EtOAc, n-BuOH and H_(2)O, successively. From the EtOAc fraction, four compounds were isolated through the repeated silica gel column chromatographies. From the results of physico-chemical data including NMR, MS and IR, the chemical structures of the compounds were determined as oleic aicd methyl ester(1), linoleic aicd methyl ester(2), β-sitosterol(3), and daucosterol(4). They were the first to be isolated from C. illinoensis.

식용식물자원으로부터 활성물질의 탐색-12. 꽃마리(Trigonotis peduncularis Benth.)로부터 Flavonol 배당체의 분리 및 hACAT1 저해활성

양혜정 ( Hye Joung Yang ),송명종 ( Myoung Chong Song ),방면호 ( Myun Ho Bang ),이진희 ( Jin Hee Lee ),정인식 ( In Sik Chung ),이윤형 ( Youn Hyung Lee ),정태숙 ( Tae Sook Jeong ),권병목 ( Byoung Mog Kwon ),김성훈 ( Sung Hoon Kim 한국응용생명화학회 2005 Applied Biological Chemistry (Appl Biol Chem) Vol.48 No.1

Cryopreservation of Embryo by Concentration of Ethylene Glycol and Day 6, 7, 8, 9 Embryo in Korean Native Cattle (Hanwoo)

Joung-Jun Park,Han-Jun Yoo,Hye-Won Choi,Ki-Soo Cheong,Ji-Tae Kim,박춘근,Boh-Suk Yang 사단법인 한국동물생명공학회 2012 한국동물생명공학회지 Vol.27 No.4

This study was carried out to effects of ethylene glycol concentration, sucrose and culture day of in vitro production embryo on slow-down freezing in Hanwoo. 6, 7, 8 and 9 day embryos produced in vitro were frozen using 1.8M EG+0.1M sucrose, 1.8M EG+0.5% BSA and 1.5M EG+0.1M sucrose media. Survivability was confirmed after frozenthawed 24 and 48h and ICM, TE cell number were counted by Hoechst 33342 and PI staining after frozen-thawed 24h. As a result, 1.8M EG+0.1M sucrose group was most significantly (p<0.05) higher compared with the other treatment groups on survivability, TE and total cell number after frozen-thawed 24h (94.2 ± 2.6%, 94.67 ± 3.4 and 129.67 ± 5.5). ICM number did not found significant (p<0.05) differences between the three treatment groups. in 6, 7, 8 and 9 day of embryos using three types of freezing media, frozen-thawed, 1.8M EG+0.1M sucrose groups with embryos cultured 8 day was significantly (p<0.05) highest survivability to 98.3 ± 1.7% after frozen-thawed 24h. 1.5M EG+0.1 sucrose group with embryos cultured 9 day was significantly higher survivability than group of embryos cultured 8 day after frozen-thawed 24 and 48h. In conclusion, 1.8M EG+0.1M sucrose media is considered to be effective to cryopreservation of embryos cultured 8 and 9 day.

Tumor Heterogeneity Predicts Metastatic Potential in Colorectal Cancer

Joung, Je-Gun,Oh, Bo Young,Hong, Hye Kyung,Al-Khalidi, Hisham,Al-Alem, Faisal,Lee, Hae-Ock,Bae, Joon Seol,Kim, Jinho,Cha, Hong-Ui,Alotaibi, Maram,Cho, Yong Beom,Hassanain, Mazen,Park, Woong-Yang,Lee, American Association for Cancer Research 2017 Clinical Cancer research Vol.23 No.23

<P><B>Purpose:</B> Tumors continuously evolve to maintain growth; secondary mutations facilitate this process, resulting in high tumor heterogeneity. In this study, we compared mutations in paired primary and metastatic colorectal cancer tumor samples to determine whether tumor heterogeneity can predict tumor metastasis.</P><P><B>Experimental Design:</B> Somatic variations in 46 pairs of matched primary-liver metastatic tumors and 42 primary tumors without metastasis were analyzed by whole-exome sequencing. Tumor clonality was estimated from single-nucleotide and copy-number variations. The correlation between clinical parameters of patients and clonal heterogeneity in liver metastasis was evaluated.</P><P><B>Results:</B> Tumor heterogeneity across colorectal cancer samples was highly variable; however, a high degree of tumor heterogeneity was associated with a worse disease-free survival. Highly heterogeneous primary colorectal cancer was correlated with a higher rate of liver metastasis. Recurrent somatic mutations in <I>APC, TP53</I>, and <I>KRAS</I> were frequently detected in highly heterogeneous colorectal cancer. The variant allele frequency of these mutations was high, while somatic mutations in other genes such as <I>PIK3CA</I> and <I>NOTCH1</I> were low. The number and distribution of primary colorectal cancer subclones were preserved in metastatic tumors.</P><P><B>Conclusions:</B> Heterogeneity of primary colorectal cancer tumors can predict the potential for liver metastasis and thus, clinical outcome of patients. <I>Clin Cancer Res; 23(23); 7209–16. ©2017 AACR</I>.</P>

Genetic Study on the Gene Cluster for Validamycin Biosynthesis

Joung-Hoon Kim(김정훈),Jong-Hee Kim(김종희),Yoon-Hee Kim(김윤희),Hye-Young Yang(양혜영),Yong-Keun Chang(장용근),Soon-Kwang Hong(홍순광) 한국생물공학회 2005 한국생물공학회 학술대회 Vol.2005 No.10

Glycosyldiglycerides from the Aerial Parts of Garland (Chrysanthemum coronarium)

송명종,Hye-Joung Yang,Do-Gyeong Lee,김대근,안은미,Young-Min Woo,Nam-In Baek 한국응용생명화학회 2009 Journal of Applied Biological Chemistry (J. Appl. Vol.52 No.1

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Cryopreservation of Embryo by Concentration of Ethylene Glycol and Day 6, 7, 8, 9 Embryo in Korean Native Cattle (Hanwoo)

Park, Joung-Jun,Yoo, Han-Jun,Choi, Hye-Won,Cheong, Ki-Soo,Kim, Ji-Tae,Park, Choon-Keun,Yang, Boh-Suk 韓國受精卵移植學會 2012 한국동물생명공학회지 Vol.27 No.4

This study was carried out to effects of ethylene glycol concentration, sucrose and culture day of in vitro production embryo on slow-down freezing in Hanwoo. 6, 7, 8 and 9 day embryos produced in vitro were frozen using 1.8M EG+0.1M sucrose, 1.8M EG+0.5% BSA and 1.5M EG+0.1M sucrose media. Survivability was confirmed after frozen-thawed 24 and 48h and ICM, TE cell number were counted by Hoechst 33342 and PI staining after frozen-thawed 24h. As a result, 1.8M EG+0.1M sucrose group was most significantly (p<0.05) higher compared with the other treatment groups on survivability, TE and total cell number after frozen-thawed 24h (, and ). ICM number did not found significant (p<0.05) differences between the three treatment groups. in 6, 7, 8 and 9 day of embryos using three types of freezing media, frozen-thawed, 1.8M EG+0.1M sucrose groups with embryos cultured 8 day was significantly (p<0.05) highest survivability to after frozen-thawed 24h. 1.5M EG+0.1 sucrose group with embryos cultured 9 day was significantly higher survivability than group of embryos cultured 8 day after frozen-thawed 24 and 48h. In conclusion, 1.8M EG+0.1M sucrose media is considered to be effective to cryopreservation of embryos cultured 8 and 9 day.

중학교 학습부진아의 수학 기피성향 치유방안

박혜숙,박기양,김영국,박규홍,박윤범,권혁천,박노경,백은정,황정연 한국수학교육학회 2004 수학교육 Vol.43 No.2

In this paper, we verified the effect and appropriateness of the scheme to cure the math. disliking disposition which is the cause of underachievement in learning. We choose 3 schools as the subject of experiment for this research. Each experiment experiment class consists of 27~30 students(underachievers) whose final test results of 1st school year in the middle school are 30~60 points. In this case, we also select some middle level students whose test results are more than 60 points for the normal experimental condition. For this research, we developed the suitable test materials to cure the mathematics disliking disposition of underachievers. We applied those test materials to the experiment schools during 2.5 months and we analysed the variation of disliking disposition, the variation of math. dislike students' number and the cure rate of the math. disliking disposition. From the results of this experimental study, we find that the factors of teacher and math recognition environment have only the significant difference of math. disliking disposition between experiment class and comparison class under the 5% significance level in one middle school. We understand that this results caused by teachers' careful advice and guidance in that middle school. We also find that the number of student who dislike mathematics decreased in two middle schools. Furthermore 50% of math. disliking dispositions are cured for 9 disliking factors in the lower grade group(the group of underachievers) and as a whole, we can see that 50% of cure rate for the 7 factors of math. disliking in two middle schools. So we can understand that the experiment of our research was performed successfully in two middle schools. In this research, we find out that the scheme to cure the disliking dispositions for the factors of math. disliking depends on the factors of teacher who take charge of cure. So teachers must take interest in and must have careful concem to students and their math. disliking.